Reviewed, UniProtKB/Swiss-Prot Q8HYB2 (GGTA1_LEMCA)

Names and origin

Protein names

Recommended name:
    N-acetyllactosaminide alpha-1,3-galactosyltransferase
    EC=2.4.1.87
Alternative name(s):
    UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase
      Short name=Galactosyltransferase

Gene names

Name:

GGTA1

Organism

Lemur catta (Ring-tailed lemur)

Taxonomic identifier

9447 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Strepsirrhini › Lemuriformes › Lemuridae › Lemur

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfer of galactose from UDP-galactose to an acceptor molecule (R).
Catalytic activity	UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis By similarity.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetyllactosaminide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	N-acetyllactosaminide alpha-1,3-galactosyltransferase		PRO_0000333702
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 25	19	Signal-anchor for type II membrane protein Potential
	Topological domain	26 – 375	350	Lumenal Potential
Sites
	Metal binding	232	1	Manganese By similarity
	Metal binding	234	1	Manganese By similarity
Amino acid modifications
	Glycosylation	300	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8HYB2-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 211F95DCB9F2BABB	FASTA	375	44,402
	10 20 30 40 50 60 MNVKGKVILS MLVVSTVMVV FWEYINSPEG SFLWIYHSKN PEVGDSSTQK GWWFPSWFHD 70 80 90 100 110 120 GTPSNQEEED IDKEKRREKE QRKEDDEEEL QLWDWFNPKK RPEVVTVTSW KAPVVWEGTY 130 140 150 160 170 180 NKAILENYYA KQKITVGLTV FAVGRYIEHY LEEFITSANR YFMVGHKVIF YIMLDDISKM 190 200 210 220 230 240 PLIELGPLRS FKVFEIKPEK RWQDISMMRM KTIGEHILAH IQHEVDFLFC MDVDQVFQDN 250 260 270 280 290 300 FGVETLGQSV AQLQAWWYKA DPDEFTYERR KESAAYIPFG EGDFYYHAAI FGGTPIQVLN 310 320 330 340 350 360 ITRECFKGIL QDKKNDIEAE WHDESHLNKY FLLNKPSKIL SPEYCWDFHI GLPSDIKIVK 370 ISWQTKHYNL VRNNV

References

[1]	"Functionally important glycosyltransferase gain and loss during catarrhine primate emergence." Koike C., Uddin M., Wildman D.E., Gray E.A., Trucco M., Starzl T.E., Goodman M. Proc. Natl. Acad. Sci. U.S.A. 104:559-564(2007) [PubMed: 17194757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY126667 mRNA. Translation: AAM95595.2.
3D structure databases
SMR	Q8HYB2. Positions 89-375.
ModBase	Search...
Protein family/group databases
CAZy	GT6. Glycosyltransferase Family 6.
Phylogenomic databases
HOVERGEN	Q8HYB2.
Enzyme and pathway databases
BRENDA	2.4.1.87. 289129.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. Glyco_trans_6. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGTA1_LEMCA

Accession

Primary (citable) accession number: Q8HYB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)