Q8HY56 (GGTA1_FELCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyllactosaminide alpha-1,3-galactosyltransferase
EC=2.4.1.87

Alternative name(s):
UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase
Short name=Galactosyltransferase

Gene names

Name:

GGTA1

Organism

Felis catus (Cat) (Felis silvestris catus)

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transfer of galactose from UDP-galactose to an acceptor molecule (R).
Catalytic activity	UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis By similarity.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetyllactosaminide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	N-acetyllactosaminide alpha-1,3-galactosyltransferase		PRO_0000333700
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 25	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	26 – 370	345	Lumenal Potential
Sites
	Metal binding	227	1	Manganese By similarity
	Metal binding	229	1	Manganese By similarity
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8HY56 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E0D908D1D61B5849	FASTA	370	43,569
	10 20 30 40 50 60 MNVKGRVVLS MLVVSTVIVV FWEYINSPEG SFLWIYHSKN PEVGDSSTQK GWWFPSWFNN 70 80 90 100 110 120 RTHSYPEEEA VDEGDEQRKE NSEELQLSDW FNPQKRPDVV TVTEWKAPVV WEGTYNKAIL 130 140 150 160 170 180 ENYYARQKIT VGLTVFAVGR YIEHYLEEFL ISANRYFMVG HKVIFYIMVD DVSKMPLIEL 190 200 210 220 230 240 GPLRSFKVFE IKPEKRWQDI SMMRMKIIGE HIVAHIQHEV DFLFCMDVDQ VFQDSFGVET 250 260 270 280 290 300 LGQSVAQLQA WWYKADPDEF TYERRKESAA YIPFGEGDFY YHAAIFGGTP TQVLNITQEC 310 320 330 340 350 360 FKGILQDKKN DIEAEWHDES HLNKYFLLNK PTKILSPEYC WDYHIGLPSD IKIVKISWQT 370 KEYNLVRNNI

References

[1]	"Isolation and phylogenetic analysis of alpha 1,3-galactosyltransferase and expression in transduced human cells." Roy B.B., Oue A., Shinagawa M., Tanaka A., Tamura K., Shimizu N., Hoshino H. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY167024 mRNA. Translation: AAN86555.1.
RefSeq	NP_001009308.1. NM_001009308.1.
3D structure databases
HSSP	HSSP built from PDB template 1G93 based on UniProtKB P14769.
ProteinModelPortal	Q8HY56.
SMR	Q8HY56. Positions 84-370.
ModBase	Search...
Protein family/group databases
CAZy	GT6. Glycosyltransferase Family 6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	493877.
Organism-specific databases
CTD	14594.
Phylogenomic databases
eggNOG	maNOG16717.
HOVERGEN	HBG003563.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. Glyco_trans_6. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGTA1_FELCA

Accession

Primary (citable) accession number: Q8HY56

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 1, 2003
Last modified:	September 21, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program