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Q8HXX5

- GSHB_MACFA

UniProt

Q8HXX5 - GSHB_MACFA

Protein

Glutathione synthetase

Gene

GSS

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251SubstrateBy similarity
    Metal bindingi144 – 1441MagnesiumBy similarity
    Binding sitei144 – 1441ATPBy similarity
    Metal bindingi146 – 1461MagnesiumBy similarity
    Binding sitei220 – 2201SubstrateBy similarity
    Binding sitei305 – 3051ATPBy similarity
    Metal bindingi368 – 3681MagnesiumBy similarity
    Binding sitei375 – 3751ATPBy similarity
    Binding sitei425 – 4251ATPBy similarity
    Binding sitei450 – 4501SubstrateBy similarity
    Binding sitei452 – 4521ATPBy similarity
    Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi364 – 37310ATPBy similarity
    Nucleotide bindingi398 – 4014ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. glutathione binding Source: UniProtKB
    3. glutathione synthase activity Source: UniProtKB-EC
    4. magnesium ion binding Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase (EC:6.3.2.3)
    Short name:
    GSH synthetase
    Short name:
    GSH-S
    Alternative name(s):
    Glutathione synthase
    Gene namesi
    Name:GSS
    ORF Names:QccE-14611
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 474473Glutathione synthetasePRO_0000211261Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ8HXX5.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8HXX5.
    SMRiQ8HXX5. Positions 3-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni148 – 1514Substrate bindingBy similarity
    Regioni214 – 2163Substrate bindingBy similarity
    Regioni267 – 2704Substrate bindingBy similarity
    Regioni461 – 4622Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the eukaryotic GSH synthase family.Curated

    Phylogenomic databases

    HOVERGENiHBG002458.
    KOiK01920.

    Family and domain databases

    Gene3Di1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProiIPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR11130. PTHR11130. 1 hit.
    PfamiPF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001558. GSHase. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8HXX5-1 [UniParc]FASTAAdd to Basket

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    MATNWGSLLQ NEQQLEELAR QAVDRALAEG VLLRTSQEPT SSEVVSYAPF    50
    TLFPSLVPSA LLEQAYAVQM DFNLLVDAVN QNAAFLEQTL SSTIEQDDFT 100
    ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QRSTDGSPAL KQIEINTISA 150
    SFGGLASRTP AVHRHVLSVL SKTKEAGKIL SNNPSKGLAL GIAKAWELYG 200
    STNALVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRTFE DISEKGSLDQ 250
    DRRLFVDGQE IAVVYFRDGY MPRQYSLQNW EARLLLERSC AAKCPDIATQ 300
    LAGTKKVQQE LSRPGMLEML LPGQPEAVAR LRATFAGLYS LDMGEEGDQA 350
    IAKALAAPSR FVLKPQREGG GNNLYGEEMV QALKQLKDSE ERASYILMEK 400
    TEPEPFENCL LRPGSPAQVV QCISELGIFG VYVRHEKTLV MNKHVGHLLR 450
    TKAIEHADGG VAAGVAVLDN PYPV 474
    Length:474
    Mass (Da):52,413
    Last modified:March 1, 2003 - v1
    Checksum:iECFB2A446B007C4D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB083314 mRNA. Translation: BAC20593.1.
    RefSeqiNP_001270338.1. NM_001283409.1.
    UniGeneiMfa.8796.

    Genome annotation databases

    GeneIDi101866287.
    KEGGimcf:101866287.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB083314 mRNA. Translation: BAC20593.1 .
    RefSeqi NP_001270338.1. NM_001283409.1.
    UniGenei Mfa.8796.

    3D structure databases

    ProteinModelPortali Q8HXX5.
    SMRi Q8HXX5. Positions 3-474.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q8HXX5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101866287.
    KEGGi mcf:101866287.

    Organism-specific databases

    CTDi 2937.

    Phylogenomic databases

    HOVERGENi HBG002458.
    KOi K01920.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .

    Family and domain databases

    Gene3Di 1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProi IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR11130. PTHR11130. 1 hit.
    Pfami PF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001558. GSHase. 1 hit.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of cDNA for macaque neurological disease genes."
      Kusuda J., Osada N., Hida M., Sugano S., Hashimoto K.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiGSHB_MACFA
    AccessioniPrimary (citable) accession number: Q8HXX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3