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Q8HXX5 (GSHB_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

Short name=GSH synthetase
Short name=GSH-S
EC=6.3.2.3
Alternative name(s):
Glutathione synthase
Gene names
Name:GSS
ORF Names:QccE-14611
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the eukaryotic GSH synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 474473Glutathione synthetase
PRO_0000211261

Regions

Nucleotide binding364 – 37310ATP By similarity
Nucleotide binding398 – 4014ATP By similarity
Region148 – 1514Substrate binding By similarity
Region214 – 2163Substrate binding By similarity
Region267 – 2704Substrate binding By similarity
Region461 – 4622Substrate binding By similarity

Sites

Metal binding1441Magnesium By similarity
Metal binding1461Magnesium By similarity
Metal binding3681Magnesium By similarity
Binding site1251Substrate By similarity
Binding site1441ATP By similarity
Binding site2201Substrate By similarity
Binding site3051ATP By similarity
Binding site3751ATP By similarity
Binding site4251ATP By similarity
Binding site4501Substrate By similarity
Binding site4521ATP By similarity
Binding site4581ATP; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8HXX5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: ECFB2A446B007C4D

FASTA47452,413
        10         20         30         40         50         60 
MATNWGSLLQ NEQQLEELAR QAVDRALAEG VLLRTSQEPT SSEVVSYAPF TLFPSLVPSA 

        70         80         90        100        110        120 
LLEQAYAVQM DFNLLVDAVN QNAAFLEQTL SSTIEQDDFT ARLFDIHKQV LKEGIAQTVF 

       130        140        150        160        170        180 
LGLNRSDYMF QRSTDGSPAL KQIEINTISA SFGGLASRTP AVHRHVLSVL SKTKEAGKIL 

       190        200        210        220        230        240 
SNNPSKGLAL GIAKAWELYG STNALVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRTFE 

       250        260        270        280        290        300 
DISEKGSLDQ DRRLFVDGQE IAVVYFRDGY MPRQYSLQNW EARLLLERSC AAKCPDIATQ 

       310        320        330        340        350        360 
LAGTKKVQQE LSRPGMLEML LPGQPEAVAR LRATFAGLYS LDMGEEGDQA IAKALAAPSR 

       370        380        390        400        410        420 
FVLKPQREGG GNNLYGEEMV QALKQLKDSE ERASYILMEK TEPEPFENCL LRPGSPAQVV 

       430        440        450        460        470 
QCISELGIFG VYVRHEKTLV MNKHVGHLLR TKAIEHADGG VAAGVAVLDN PYPV 

« Hide

References

[1]"Isolation and characterization of cDNA for macaque neurological disease genes."
Kusuda J., Osada N., Hida M., Sugano S., Hashimoto K.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB083314 mRNA. Translation: BAC20593.1.
RefSeqNP_001270338.1. NM_001283409.1.
UniGeneMfa.8796.

3D structure databases

ProteinModelPortalQ8HXX5.
SMRQ8HXX5. Positions 3-474.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ8HXX5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101866287.
KEGGmcf:101866287.

Organism-specific databases

CTD2937.

Phylogenomic databases

HOVERGENHBG002458.
KOK01920.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR11130. PTHR11130. 1 hit.
PfamPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001558. GSHase. 1 hit.
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01986. glut_syn_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGSHB_MACFA
AccessionPrimary (citable) accession number: Q8HXX5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways