Reviewed, UniProtKB/Swiss-Prot Q8HXX4 (ECHB_MACFA)

Names and origin

Protein names

Recommended name:
    Trifunctional enzyme subunit beta, mitochondrial
Alternative name(s):
    TP-beta
Including the following 1 domains:
    1- Recommended name:
            3-ketoacyl-CoA thiolase
              EC=2.3.1.16
        Alternative name(s):
            Acetyl-CoA acyltransferase
            Beta-ketothiolase

Gene names

Name:	HADHB
ORF Names:	QtrA-13435

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit structure	Octamer of 4 alpha (HADHA) and 4 beta (HADHB) subunits By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion By similarity
	Chain	35 – 475	441	Trifunctional enzyme subunit beta, mitochondrial		PRO_0000034081
Sites
	Active site	139	1	Acyl-thioester intermediate By similarity
	Active site	429	1	Proton acceptor By similarity
	Active site	459	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	73	1	N6-acetyllysine By similarity
	Modified residue	189	1	N6-acetyllysine By similarity
	Modified residue	202	1	N6-acetyllysine By similarity
	Modified residue	349	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8HXX4-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 411A85F52B2502DA	FASTA	475	51,356
	10 20 30 40 50 60 MTTILTCPFK KLPTTSKWAL RFAIRPLSCS SQLRAAPAVQ TKTKKTLAKP NIRNVVVVDG 70 80 90 100 110 120 VRTPFLLSGT SYKDLMPHDL ARAALTGLLH RTSVPKEVVD YIIFGTVIQE VKTSNVAREA 130 140 150 160 170 180 ALGAGFSDKT PAHTVTMACI SANQAMTTGV GLIASGQCDV IVAGGVELMS DIPIRHSRKM 190 200 210 220 230 240 RKLMLDLNKA KSMGQRLSLI SKFRLNFLAP ELPAVAEFST SETMGHSADR LAAAFAVSRL 250 260 270 280 290 300 EQDEYALRSH SLAKKAQDEG LLSDVVPFRV PGKDTVTKDN GIRPSSLEQM AKLKPAFIKP 310 320 330 340 350 360 YGTVTAANSS FLTDGASAML IMAEEKALAM GYKPKAYLRD FMYVSQDPKD QLLLGPTYAT 370 380 390 400 410 420 PKVLEKAGLT MNDIDAFEFH EAFSGQILAN FKAMDSDWFA ENYMGRKTKV GLPPLEKFNN 430 440 450 460 470 WGGSLSLGHP FGATGCRLVM AAANRLRKEG GQYGLVAACA AGGQGHAMIV EAYPK

References

[1]	"Isolation and characterization of cDNA for macaque neurological disease genes." Kusuda J., Osada N., Hida M., Sugano S., Hashimoto K. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Temporal cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB083315 mRNA. Translation: BAC20594.1.
3D structure databases
HSSP	HSSP built from PDB template 1AFW based on UniProtKB P27796.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q8HXX4.
Enzyme and pathway databases
BRENDA	2.3.1.16. 3438.
Family and domain databases
InterPro	IPR002155. Thiolase. [Graphical view]
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECHB_MACFA

Accession

Primary (citable) accession number: Q8HXX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	March 1, 2003
Last modified:	October 13, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)