Q8HXW6 (PPT1_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyl-protein thioesterase 1
Short name=PPT-1
EC=3.1.2.22

Alternative name(s):
Palmitoyl-protein hydrolase 1

Gene names

Name:	PPT1
ORF Names:	QnpA-18851

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons By similarity.
Catalytic activity	Palmitoyl-protein + H2O = palmitate + protein.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the palmitoyl-protein thioesterase family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	DNA fragmentation involved in apoptotic nuclear change Inferred from sequence or structural similarity. Source: UniProtKB cofactor metabolic process Inferred from sequence or structural similarity. Source: UniProtKB cofactor transport Inferred from sequence or structural similarity. Source: UniProtKB lipid catabolic process Inferred from sequence or structural similarity. Source: UniProtKB lysosomal lumen acidification Inferred from sequence or structural similarity. Source: UniProtKB membrane raft organization Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of neuron apoptosis Inferred from sequence or structural similarity. Source: UniProtKB nervous system development Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of pinocytosis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of receptor-mediated endocytosis Inferred from sequence or structural similarity. Source: UniProtKB protein depalmitoylation Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB axon Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from sequence or structural similarity. Source: UniProtKB extracellular region Inferred from sequence or structural similarity. Source: UniProtKB lysosome Inferred from sequence or structural similarity. Source: UniProtKB membrane fraction Inferred from sequence or structural similarity. Source: UniProtKB membrane raft Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB synaptic vesicle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	palmitoyl-(protein) hydrolase activity Inferred from sequence or structural similarity. Source: UniProtKB palmitoyl-CoA hydrolase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Chain	28 – 306	279	Palmitoyl-protein thioesterase 1		PRO_0000025551
Sites
	Active site	115	1	By similarity
	Active site	233	1	By similarity
	Active site	289	1	By similarity
Amino acid modifications
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	232	1	N-linked (GlcNAc...) Potential
	Disulfide bond	45 ↔ 46		By similarity
	Disulfide bond	96 ↔ 128		By similarity
	Disulfide bond	152 ↔ 160		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8HXW6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1E0C5EE0EF15AB78	FASTA	306	34,247
	10 20 30 40 50 60 MASPSCLWLL AVALLPWTCA ARALHHLDPP APLPLVIWHG MGDSCCNPLS MGAIKKMVEK 70 80 90 100 110 120 KIPGIYVLSL EIGKTLMEDV ENSFFLNVNS QVTTVCQTLA KDPKLQQGYN AMGFSQGGQF 130 140 150 160 170 180 LRAVAQRCPS PPMINLISVG GQHQGVFGLP RCPGESSHIC DFIRKTLNAG AYSKVVQERL 190 200 210 220 230 240 VQAEYWHDPI KEDVYRNHSI FLADINQERG INESYKKNLM ALKKFVMVKF LNDSIVDPVD 250 260 270 280 290 300 SEWFGFYRSG QAKETIPLQE TSLYTQDRLG LKEMDNAGQL VFLATEGDHL QLSEEWFYAH IIPFLG

References

[1]	"Isolation and characterization of cDNA for macaque neurological disease genes." Kusuda J., Osada N., Hida M., Sugano S., Hashimoto K. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Parietal cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB083325 mRNA. Translation: BAC20604.1.
3D structure databases
ProteinModelPortal	Q8HXW6.
SMR	Q8HXW6. Positions 28-305.
ModBase	Search...
Proteomic databases
PRIDE	Q8HXW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG018186.
OrthoDB	EOG4TB4C0.
Family and domain databases
InterPro	IPR002472. Palm_thioest. [Graphical view]
Pfam	PF02089. Palm_thioest. 1 hit. [Graphical view]
PRINTS	PR00414. PPTHIESTRASE.
ProtoNet	Search...

Entry information

Entry name

PPT1_MACFA

Accession

Primary (citable) accession number: Q8HXW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	March 1, 2003
Last modified:	October 19, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program