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Q8HXW0 (GGLO_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-gulonolactone oxidase
Short name=LGO
EC=1.1.3.8
Alternative name(s):
L-gulono-gamma-lactone oxidase
Short name=GLO
Gene names
Name:GULO
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate By similarity. UniProtKB P10867

Catalytic activity

L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2.

Cofactor

FAD By similarity. UniProtKB P10867

Pathway

Cofactor biosynthesis; L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway; L-ascorbate from UDP-alpha-D-glucuronate: step 4/4.

Subcellular location

Microsome membrane; Single-pass membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.

Sequence similarities

Belongs to the oxygen-dependent FAD-linked oxidoreductase family.

Contains 1 FAD-binding PCMH-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity UniProtKB P10867
Chain2 – 440439L-gulonolactone oxidase
PRO_0000128160

Regions

Transmembrane253 – 27321Helical; Potential
Domain17 – 187171FAD-binding PCMH-type

Amino acid modifications

Modified residue541Pros-8alpha-FAD histidine By similarity UniProtKB P08159

Sequences

Sequence LengthMass (Da)Tools
Q8HXW0 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 81BC3E0873627201

FASTA44050,352
        10         20         30         40         50         60 
MVHGHKGVKF QNWAKTYGCC PEMYYQPTSV EEIREVLALA RQQNKRVKVV GGGHSPSDIA 

        70         80         90        100        110        120 
CTDGFMIHMG KMNRVLKVDM EKKQVTVEAG ILLADLHPQL DKHGLALSNL GAVSDVTAGG 

       130        140        150        160        170        180 
VIGSGTHNTG IKHGILATQV VELTLLTPDG TVLVCSESSN AEVFQAARVH LGCLGVILTV 

       190        200        210        220        230        240 
TLQCVPQFHL QETTFPSTLK EVLDNLDSHL KKSEYFRFLW FPHSENVSVI YQDHTNKPPS 

       250        260        270        280        290        300 
SSANWFWDYA IGFYLLEFLL WISTFVPGLV GWINRFFFWL LFNGKKENCN LSHKIFTYEC 

       310        320        330        340        350        360 
RFKQHVQDWA IPREKTKEAL LELKAMLEAH PKVVAHYPVE VRFTRADDIL LSPCFQRDSC 

       370        380        390        400        410        420 
YMNIIMYRPY GKDVPRLDYW LAYETIMKKV GGRPHWAKAH NCTRKDFEKM YPAFRKFCAI 

       430        440 
REKLDPTGMF LNAYLEKVFY

« Hide

References

[1]"Intragenic deletion in the gene encoding L-gulonolactone oxidase causes vitamin C deficiency in pigs."
Hasan L., Voegeli P., Stoll P., Kramer S.S., Stranzinger G., Neuenschwander S.
Mamm. Genome 15:323-333(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF440259 mRNA. Translation: AAN63634.1.
AF136938 mRNA. Translation: AAF61429.1.
RefSeqNP_001123420.1. NM_001129948.1.
UniGeneSsc.16369.

3D structure databases

ProteinModelPortalQ8HXW0.
ModBaseSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000010324.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000010600; ENSSSCP00000010324; ENSSSCG00000009667.
GeneID396759.
KEGGssc:396759.

Organism-specific databases

CTD268756.

Phylogenomic databases

eggNOGCOG0277.
GeneTreeENSGT00510000049722.
HOGENOMHOG000252847.
HOVERGENHBG005834.
KOK00103.
OMATYGKLQN.
OrthoDBEOG4RNB8D.

Enzyme and pathway databases

UniPathwayUPA00991; UER00939.

Family and domain databases

Gene3D3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProIPR007173. ALO.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR010031. FAD_lactone_oxidase.
IPR010032. FAD_linked_OxRdtase_bac.
IPR023595. LGO_GLO.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
[Graphical view]
PfamPF04030. ALO. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
PIRSFPIRSF000136. LGO_GLO. 1 hit.
SUPFAMSSF56176. FAD-binding_2. 1 hit.
TIGRFAMsTIGR01679. bact_FAD_ox. 1 hit.
TIGR01678. FAD_lactone_ox. 1 hit.
PROSITEPS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGGLO_PIG
AccessionPrimary (citable) accession number: Q8HXW0
Secondary accession number(s): Q9N1U4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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