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Protein

Cleavage stimulation factor subunit 2

Gene

CSTF2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage stimulation factor subunit 2
Alternative name(s):
CF-1 64 kDa subunit
Cleavage stimulation factor 64 kDa subunit
Short name:
CSTF 64 kDa subunit
Short name:
CstF-64
Gene namesi
Name:CSTF2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Nucleus By similarity

  • Note: Localized with DDX1 in cleavage bodies.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815301 – 572Cleavage stimulation factor subunit 2Add BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Cross-linki189Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei308Omega-N-methylarginineBy similarity1
Modified residuei463Omega-N-methylarginineBy similarity1
Modified residuei470Omega-N-methylarginineBy similarity1
Modified residuei519PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8HXM1.
PeptideAtlasiQ8HXM1.
PRIDEiQ8HXM1.

Expressioni

Gene expression databases

BgeeiENSBTAG00000003547.

Interactioni

Subunit structurei

The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells (By similarity). Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004619.

Structurei

3D structure databases

ProteinModelPortaliQ8HXM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 94RRMPROSITE-ProRule annotationAdd BLAST79
Repeati410 – 4141; approximate5
Repeati415 – 41925
Repeati420 – 4243; approximate5
Repeati425 – 4294; approximate5
Repeati430 – 43455
Repeati435 – 43965
Repeati440 – 44475
Repeati445 – 44985
Repeati450 – 4549; approximate5
Repeati455 – 459105
Repeati460 – 46411; approximate5

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 248Interactions with CSTF3 and SYMPKBy similarityAdd BLAST141
Regioni410 – 46411 X 5 AA tandem repeats of M-E-A-R-[AG]Add BLAST55
Regioni509 – 572Interaction with RPO2TC1By similarityAdd BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi198 – 409Gly/Pro-richAdd BLAST212
Compositional biasi465 – 521Gly/Pro-richAdd BLAST57

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XQBV. LUCA.
HOGENOMiHOG000214373.
HOVERGENiHBG051145.
InParanoidiQ8HXM1.
KOiK14407.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033105. CSTF2.
IPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF57. PTHR23139:SF57. 1 hit.
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8HXM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLTVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYD
60 70 80 90 100
RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE
110 120 130 140 150
LKSLGTGAPV IESPYGETIS PEDAPESISK AVASLPPEQM FELMKQMKLC
160 170 180 190 200
VQNSPQEARN MLLQNPQLAY ALLQAQVVMR IVDPEIALKI LHRQTNIPTL
210 220 230 240 250
IAGNPQTVHS AGPGSGSSVS MNQQNPQTPQ AQTLSGMHVN GAPPLMQASL
260 270 280 290 300
QAGVAAPGQI PATVTGPGPG SLAPAGGMQA QVGMPGSGPV SMERGQVPMQ
310 320 330 340 350
DPRAAMQRGP LPANVPTPRG LLGDAPNDPR GGTLLSVTGE VEPRGYLGPP
360 370 380 390 400
HQGPPMHHVP GHDSRGPPPH EMRGGPLTEP RPLMAEPRGP MIDQRGPPLD
410 420 430 440 450
GRGGRDPRGI DARAMEARGL DARGLEARAM EARAMEARAM EARAMEARAM
460 470 480 490 500
EVRGMEARSM DTRGPVPGPR GPMPSGIQGP SPINMGAVGP QGSRQVPVMQ
510 520 530 540 550
GAGMQGASIQ GGGQPGGFSP GQNQVTPQDH EKAALIMQVL QLTADQIAML
560 570
PPEQRQSILI LKEQIQKSTG AP
Length:572
Mass (Da):60,438
Last modified:March 1, 2003 - v1
Checksum:i809140409D380209
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY130297 mRNA. Translation: AAN05427.1.
RefSeqiNP_777110.1. NM_174685.2.
UniGeneiBt.78724.

Genome annotation databases

GeneIDi282588.
KEGGibta:282588.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY130297 mRNA. Translation: AAN05427.1.
RefSeqiNP_777110.1. NM_174685.2.
UniGeneiBt.78724.

3D structure databases

ProteinModelPortaliQ8HXM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004619.

Proteomic databases

PaxDbiQ8HXM1.
PeptideAtlasiQ8HXM1.
PRIDEiQ8HXM1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282588.
KEGGibta:282588.

Organism-specific databases

CTDi1478.

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XQBV. LUCA.
HOGENOMiHOG000214373.
HOVERGENiHBG051145.
InParanoidiQ8HXM1.
KOiK14407.

Gene expression databases

BgeeiENSBTAG00000003547.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033105. CSTF2.
IPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF57. PTHR23139:SF57. 1 hit.
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSTF2_BOVIN
AccessioniPrimary (citable) accession number: Q8HXM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.