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Protein

Thiopurine S-methyltransferase

Gene

TPMT

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40SubstrateBy similarity1
Binding sitei69S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei90S-adenosyl-L-methionineBy similarity1
Binding sitei152S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-CFA-156581. Methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiopurine S-methyltransferase (EC:2.1.1.67)
Alternative name(s):
Thiopurine methyltransferase
Gene namesi
Name:TPMT
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 35

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002200971 – 245Thiopurine S-methyltransferaseAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8HX86.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000014924.

Structurei

3D structure databases

ProteinModelPortaliQ8HX86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 40S-adenosyl-L-methionine bindingBy similarityAdd BLAST12

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IGTR. Eukaryota.
ENOG4111GNF. LUCA.
GeneTreeiENSGT00390000016823.
HOGENOMiHOG000276919.
HOVERGENiHBG003037.
InParanoidiQ8HX86.
KOiK00569.
OMAiKWVNGKT.
OrthoDBiEOG091G0VS2.
TreeFamiTF328951.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00812. Thiopur_methtran. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025835. Thiopurine_S-MeTrfase.
IPR008854. TPMT.
[Graphical view]
PfamiPF05724. TPMT. 1 hit.
[Graphical view]
PIRSFiPIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51585. SAM_MT_TPMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8HX86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKTRTFLDV KEYPDTEVQK NRVLTLEEWQ EKWVSRRIGF HQEQGHKLLK
60 70 80 90 100
KHLDTFLKGE NGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIREFF
110 120 130 140 150
AEQNLSYTEE PIVEIPGGKI FKSSSGNISL YCCSLFDLPR ANIGKFDRIW
160 170 180 190 200
DRGALVAINP GDRERYADIM LSLTRKGFHY LLAVLCYDPT KHAGPPFYVP
210 220 230 240
EAEIKKLFGS ICNIHCLEKV DVFEEQHKSW GIDYIIEKLY LFTEK
Length:245
Mass (Da):28,428
Last modified:March 1, 2003 - v1
Checksum:iFF248133D3F8EF5A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti97R → G.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057077 mRNA. Translation: AAL18009.1.
AY057087
, AY057080, AY057081, AY057082, AY057083, AY057084, AY057085, AY057086 Genomic DNA. Translation: AAL18006.1.
RefSeqiNP_001003015.1. NM_001003015.1.
XP_005640012.1. XM_005639955.2.
XP_005640013.1. XM_005639956.2.
XP_013965954.1. XM_014110479.1.
UniGeneiCfa.33.

Genome annotation databases

EnsembliENSCAFT00000016138; ENSCAFP00000014924; ENSCAFG00000010160.
ENSCAFT00000016139; ENSCAFP00000014925; ENSCAFG00000010160.
GeneIDi403536.
KEGGicfa:403536.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057077 mRNA. Translation: AAL18009.1.
AY057087
, AY057080, AY057081, AY057082, AY057083, AY057084, AY057085, AY057086 Genomic DNA. Translation: AAL18006.1.
RefSeqiNP_001003015.1. NM_001003015.1.
XP_005640012.1. XM_005639955.2.
XP_005640013.1. XM_005639956.2.
XP_013965954.1. XM_014110479.1.
UniGeneiCfa.33.

3D structure databases

ProteinModelPortaliQ8HX86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000014924.

Proteomic databases

PaxDbiQ8HX86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000016138; ENSCAFP00000014924; ENSCAFG00000010160.
ENSCAFT00000016139; ENSCAFP00000014925; ENSCAFG00000010160.
GeneIDi403536.
KEGGicfa:403536.

Organism-specific databases

CTDi7172.

Phylogenomic databases

eggNOGiENOG410IGTR. Eukaryota.
ENOG4111GNF. LUCA.
GeneTreeiENSGT00390000016823.
HOGENOMiHOG000276919.
HOVERGENiHBG003037.
InParanoidiQ8HX86.
KOiK00569.
OMAiKWVNGKT.
OrthoDBiEOG091G0VS2.
TreeFamiTF328951.

Enzyme and pathway databases

ReactomeiR-CFA-156581. Methylation.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00812. Thiopur_methtran. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025835. Thiopurine_S-MeTrfase.
IPR008854. TPMT.
[Graphical view]
PfamiPF05724. TPMT. 1 hit.
[Graphical view]
PIRSFiPIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51585. SAM_MT_TPMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPMT_CANLF
AccessioniPrimary (citable) accession number: Q8HX86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: March 1, 2003
Last modified: October 5, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.