Q8HX86 (TPMT_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:

TPMT

Organism

Canis familiaris (Dog) (Canis lupus familiaris)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine By similarity.
Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Thiopurine S-methyltransferase		PRO_0000220097
Sites
	Binding site	33	1	S-adenosyl-L-methionine By similarity
	Binding site	69	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	90	1	S-adenosyl-L-methionine By similarity
	Binding site	152	1	S-adenosyl-L-methionine By similarity
Amino acid modifications
	Modified residue	58	1	N6-acetyllysine By similarity
Natural variations
	Natural variant	97	1	R → G. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8HX86 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: FF248133D3F8EF5A	FASTA	245	28,428
	10 20 30 40 50 60 MDKTRTFLDV KEYPDTEVQK NRVLTLEEWQ EKWVSRRIGF HQEQGHKLLK KHLDTFLKGE 70 80 90 100 110 120 NGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIREFF AEQNLSYTEE PIVEIPGGKI 130 140 150 160 170 180 FKSSSGNISL YCCSLFDLPR ANIGKFDRIW DRGALVAINP GDRERYADIM LSLTRKGFHY 190 200 210 220 230 240 LLAVLCYDPT KHAGPPFYVP EAEIKKLFGS ICNIHCLEKV DVFEEQHKSW GIDYIIEKLY LFTEK

References

[1]

"Canine red blood cell thiopurine S-methyltransferase: companion animal pharmacogenetics." Salavaggione O.E., Kidd L., Prondzinski J.L., Szumlanski C.L., Pankratz V.S., Wang L., Trepanier L., Weinshilboum R.M. Pharmacogenetics 12:713-724(2002) [PubMed: 12464800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT GLY-97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY057077 mRNA. Translation: AAL18009.1. AY057087 , AY057080, AY057081, AY057082, AY057083, AY057084, AY057085, AY057086 Genomic DNA. Translation: AAL18006.1.
RefSeq	NP_001003015.1. NM_001003015.1.
UniGene	Cfa.33.
3D structure databases
ProteinModelPortal	Q8HX86.
SMR	Q8HX86. Positions 14-245.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8HX86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000016138; ENSCAFP00000014924; ENSCAFG00000010160.
GeneID	403536.
KEGG	cfa:403536.
Organism-specific databases
CTD	7172.
Phylogenomic databases
eggNOG	maNOG10315.
GeneTree	ENSGT00390000016823.
HOVERGEN	HBG003037.
InParanoid	Q8HX86.
OMA	QGDIFTL.
OrthoDB	EOG4C5CKF.
PhylomeDB	Q8HX86.
Family and domain databases
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
KO	K00569.
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_CANFA

Accession

Primary (citable) accession number: Q8HX86

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	March 1, 2003
Last modified:	November 16, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program