Reviewed, UniProtKB/Swiss-Prot Q8HUH0 (RPC2B_CHLRE)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit beta' C-terminal section
    EC=2.7.7.6
Alternative name(s):
    PEP
    Plastid-encoded RNA polymerase beta' C-terminal section
      Short name=RNA polymerase beta' C-terminal section

Gene names

Name:

rpoC1B

Encoded on

Plastid; Chloroplast

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01323
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01323
Subunit structure	In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity. HAMAP MF_01323
Subcellular location	Plastid › chloroplast HAMAP MF_01323.
Miscellaneous	In C.reinhardtii the gene for this protein is split in two. HAMAP MF_01323
Sequence similarities	Belongs to the RNA polymerase beta' chain family. RpoC1 subfamily.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Chloroplast DNA-directed RNA polymerase Plastid
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	DNA-directed RNA polymerase subunit beta' C-terminal section HAMAP MF_01323		PRO_0000067867

Sequences

	Sequence		Length	Mass (Da)
	Q8HUH0-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1912E0FB97448C47	FASTA	489	55,630
	10 20 30 40 50 60 MSGQFTISDL NRLYQRIIYR NERLKKFLKD PALSSSFEMK YAQRLLQEAV DNLIQNGKSG 70 80 90 100 110 120 VVPEKDARGR LLKSLSDILK GKQGRFRQYL LGKRVDYSGR SVIVVGPRLR LHECGIPKEM 130 140 150 160 170 180 ALVLYSPFLI KRILNEKLAD TYLSAKKLIR TNPLLVSQLL REIMKSCPVL LNRAPTLHRL 190 200 210 220 230 240 GFQAFQPKLV DGKAILLHPL VCPAFNADFD GDQMAVHVPI TFEARAEAWK LMLARNNLLS 250 260 270 280 290 300 PATGEPLILP SQDMVLGCYY LTTNCAEKWS KLKKGSGMYF HNIHEVLKAY NQHLIHLHAV 310 320 330 340 350 360 IWVNIQGHLE TANNIEQPLE IRIPLNQKYF YSQKGSYTAL AGRPWQLLLR EDAYSSNGLI 370 380 390 400 410 420 AEANAGNLPQ INVAEGEVNK NILDLRYLNL NYMEIYSKTH NLLNVNHSTL VAQRGQIKDN 430 440 450 460 470 480 IHLPQLTTQI IRTTPGKILF NIIVKNAIEK RPKLLSKSNF KGGFIKNKLV NAIDQEIDNY FIRKNTALS

References

[1]	"The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats." Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B. Plant Cell 14:2659-2679(2002) [PubMed: 12417694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION, COMPLETE PLASTID GENOME.
[2]	"Nucleotide diversity in the chloroplast genome of Chlamydomonas reinhardtii." United States Department of Energy Joint Genome Institute Smith D.R. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CC-503.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF541870 Genomic DNA. Translation: AAN17821.1. FJ423446 Genomic DNA. Translation: ACJ50156.1. BK000554 Genomic DNA. Translation: DAA00969.1.
RefSeq	NP_958425.1.
3D structure databases
SMR	Q8HUH0. Positions 7-264.
ModBase	Search...
Genome annotation databases
GeneID	2717049.
KEGG	cre:ChreCp069.
Enzyme and pathway databases
BRENDA	2.7.7.6. 144.
Family and domain databases
HAMAP	MF_01323. RNApol_bact_RpoC1. Divergent sequence. [Tree]
InterPro	IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 2 hits. PF04983. RNA_pol_Rpb1_3. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPC2B_CHLRE

Accession

Primary (citable) accession number: Q8HUH0
Secondary accession number(s): B7U1K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)