Reviewed, UniProtKB/Swiss-Prot Q8HUG9 (RPC1A_CHLRE)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit beta' N-terminal section
    EC=2.7.7.6
Alternative name(s):
    PEP
    Plastid-encoded RNA polymerase beta' N-terminal section
      Short name=RNA polymerase beta' N-terminal section

Gene names

Name:

rpoC1A

Encoded on

Plastid; Chloroplast

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01323
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01323
Subunit structure	In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Subcellular location	Plastid › chloroplast. HAMAP MF_01323
Miscellaneous	In C.reinhardtii the gene for this protein is split in two. HAMAP MF_01323
Sequence similarities	Belongs to the RNA polymerase beta' chain family. RpoC1 subfamily.
Sequence caution	The sequence ACJ50157.1 differs from that shown. Reason: Frameshift at position 586.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Chloroplast DNA-directed RNA polymerase Plastid
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 603	603	DNA-directed RNA polymerase subunit beta' N-terminal section HAMAP MF_01323		PRO_0000067866

Sequences

	Sequence		Length	Mass (Da)
	Q8HUG9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1E070C22CA86F373	FASTA	603	68,613
	10 20 30 40 50 60 MHYKKYAELL KNGTYFSHIH SPTLLYPKGK VMYAQYGNSP LQGLNSQVTN LNLIRKVVAA 70 80 90 100 110 120 SAEMDSFKKL PNLQNNKLPL NNLKARLNTK AIYISNEEAQ LTVSKIGTPN AIETDVPGTI 130 140 150 160 170 180 KNAPSNTLLT HRSFKFFVNK IYAKPLISLK KTKETKPLLL GKTGTTLTQK GLNPFQSLFL 190 200 210 220 230 240 NTKSSSPSTA RSFGTKNIVN TLQIKKIVHK FENSYSKLTE INLITINLAS ANRIRQWAEK 250 260 270 280 290 300 TLPNGKVVGE VINPETIHYK TLKPIKGGLF CERIFGPLKD HECACGKKFN IKNYLTKTVN 310 320 330 340 350 360 NSSIQPIAES RQTQPNTQLS LNLQKRYFCR ICDVEYTYSI IRRTQLGYIQ LASPTTHVWF 370 380 390 400 410 420 VKGIPSYISI LLDMKKKHLQ GITYNTETLT LENSFRGRQL LPVSPSSIFE SWQKIMKKQY 430 440 450 460 470 480 PEKYNLTNTM IKIKSTNTLP LRVSQPNSNT SYSYMPNIYI PEGEGEEKTK TKLKKTTPLN 490 500 510 520 530 540 AIAQKGVKYK KPKTKKALYK QYLKNYYARK PKNSNQAEAV SFGVNKVQLP LTAFQQRNKV 550 560 570 580 590 600 YFYKSEKRNW PVLTTVAKMQ YIVSKKGWFK LIQYVIKSAE SYGAATPTKM DGLQKMSLLT KQA

References

[1]	"The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats." Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B. Plant Cell 14:2659-2679(2002) [PubMed: 12417694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION, COMPLETE PLASTID GENOME.
[2]	"Nucleotide diversity in the chloroplast genome of Chlamydomonas reinhardtii." United States Department of Energy Joint Genome Institute Smith D.R. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CC-503.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF541870 Genomic DNA. Translation: AAN17822.1. FJ423446 Genomic DNA. Translation: ACJ50157.1. Frameshift. BK000554 Genomic DNA. Translation: DAA00970.1.
RefSeq	NP_958426.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q8HUG9.
Genome annotation databases
GeneID	2717050.
KEGG	cre:ChreCp070.
Enzyme and pathway databases
BRENDA	2.7.7.6. 144.
Family and domain databases
HAMAP	MF_01323. Divergent sequence. [Tree]
InterPro	IPR007080. RNA_pol_Rpb1_1. [Graphical view]
Gene3D	G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPC1A_CHLRE

Accession

Primary (citable) accession number: Q8HUG9
Secondary accession number(s): B7U1L0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)