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Protein

UDP-arabinopyranose mutase 1

Gene

UAM1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf). Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 90:10. Is probably active as heteromer in vivo.4 Publications

Catalytic activityi

UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose.3 Publications

Cofactori

Mn2+1 Publication, Mg2+1 Publication

Kineticsi

  1. KM=22.8 µM for UDP-L-arabinofuranose1 Publication
  2. KM=45.4 µM for UDP-L-arabinopyranose1 Publication
  1. Vmax=0.688 µmol/min/mg enzyme with UDP-L-arabinofuranose as substrate1 Publication
  2. Vmax=0.269 µmol/min/mg enzyme with UDP-L-arabinofuranose as substrate1 Publication

pH dependencei

Optimum pH is 5.5-6.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei158 – 1581Required for activity
Sitei165 – 1651Required for activity

GO - Molecular functioni

  • intramolecular transferase activity Source: UniProtKB
  • UDP-arabinopyranose mutase activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15774.
BRENDAi5.4.99.30. 4460.

Protein family/group databases

CAZyiGT75. Glycosyltransferase Family 75.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-arabinopyranose mutase 1 (EC:5.4.99.30)
Short name:
OsUAM1
Alternative name(s):
Reversibly glycosylated polypeptide 1
UDP-L-arabinose mutase 1
Gene namesi
Name:UAM1
Synonyms:RGP1
Ordered Locus Names:Os03g0599800, LOC_Os03g40270
ORF Names:OJ1523_A02.1, OsJ_11657
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi112 – 1121D → N: Loss of activity. 1 Publication
Mutagenesisi151 – 1511R → A: No effect on the activity. 1 Publication
Mutagenesisi158 – 1581R → A: Loss of activity. 1 Publication
Mutagenesisi165 – 1651R → A or K: Decreases activity 20-fold. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364UDP-arabinopyranose mutase 1PRO_0000410989Add
BLAST

Post-translational modificationi

Reversibly glycosylated in vitro at Arg-158 by UDP-glucose. Reversibly glycosylated by UDP-xylose and UDP-galactose.3 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8H8T0.
PRIDEiQ8H8T0.

Expressioni

Gene expression databases

ExpressionAtlasiQ8H8T0. baseline and differential.
GenevisibleiQ8H8T0. OS.

Interactioni

Subunit structurei

Heteromers with UAM2 and UAM3.2 Publications

Protein-protein interaction databases

STRINGi39947.LOC_Os03g40270.1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi110 – 1123DXD motif

Domaini

The conserved DXD motif is involved in enzyme activity.1 Publication

Sequence similaritiesi

Belongs to the RGP family.Curated

Phylogenomic databases

eggNOGiENOG410IHTP. Eukaryota.
ENOG411144T. LUCA.
HOGENOMiHOG000234443.
InParanoidiQ8H8T0.
OMAiIDPYFAK.
OrthoDBiEOG09360C9G.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR004901. RGP_fam.
[Graphical view]
PfamiPF03214. RGP. 1 hit.
[Graphical view]
PIRSFiPIRSF016429. UPTG. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8H8T0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTVTVPSA SVPSTPLLKD ELDIVIPTIR NLDFLEMWRP FFQPYHLIIV
60 70 80 90 100
QDGDPTKTIR VPEGFDYELY NRNDINRILG PKASCISFKD SACRCFGYMV
110 120 130 140 150
SKKKYVFTID DDCFVAKDPS GKDINALEQH IKNLLSPSTP FFFNTLYDPY
160 170 180 190 200
REGADFVRGY PFSLREGAKT AVSHGLWLNI PDYDAPTQMV KPRERNSRYV
210 220 230 240 250
DAVMTVPKGT LFPMCGMNLA FDRDLIGPAM YFGLMGDGQP IGRYDDMWAG
260 270 280 290 300
WCMKVICDHL SLGVKTGLPY IWHSKASNPF VNLKKEYKGI FWQEDIIPFF
310 320 330 340 350
QNATIPKECD TVQKCYLSLA EQVREKLGKI DPYFVKLADA MVTWIEAWDE
360
LNPSTAAVEN GKAK
Length:364
Mass (Da):41,349
Last modified:March 1, 2003 - v1
Checksum:i8CB8E232A780934C
GO

Sequence cautioni

The sequence ABF97477 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA09469 differs from that shown. Reason: Frameshift at position 315. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831A → G in CAA09469 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294725 mRNA. Translation: AAG17438.1.
AJ011078 mRNA. Translation: CAA09469.1. Frameshift.
EU267966 mRNA. Translation: ACA50488.1.
GQ848047 mRNA. Translation: ADM86860.1.
AC090874 Genomic DNA. Translation: AAN08217.1.
DP000009 Genomic DNA. Translation: ABF97476.1.
DP000009 Genomic DNA. Translation: ABF97477.1. Different initiation.
AP008209 Genomic DNA. Translation: BAF12531.1.
AP014959 Genomic DNA. Translation: BAS85190.1.
CM000140 Genomic DNA. Translation: EEE59464.1.
AK061813 mRNA. Translation: BAG88123.1.
AK098933 mRNA. Translation: BAG93819.1.
RefSeqiXP_015631326.1. XM_015775840.1.
UniGeneiOs.5039.

Genome annotation databases

EnsemblPlantsiOS03T0599800-01; OS03T0599800-01; OS03G0599800.
GeneIDi4333393.
GrameneiOS03T0599800-01; OS03T0599800-01; OS03G0599800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294725 mRNA. Translation: AAG17438.1.
AJ011078 mRNA. Translation: CAA09469.1. Frameshift.
EU267966 mRNA. Translation: ACA50488.1.
GQ848047 mRNA. Translation: ADM86860.1.
AC090874 Genomic DNA. Translation: AAN08217.1.
DP000009 Genomic DNA. Translation: ABF97476.1.
DP000009 Genomic DNA. Translation: ABF97477.1. Different initiation.
AP008209 Genomic DNA. Translation: BAF12531.1.
AP014959 Genomic DNA. Translation: BAS85190.1.
CM000140 Genomic DNA. Translation: EEE59464.1.
AK061813 mRNA. Translation: BAG88123.1.
AK098933 mRNA. Translation: BAG93819.1.
RefSeqiXP_015631326.1. XM_015775840.1.
UniGeneiOs.5039.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os03g40270.1.

Protein family/group databases

CAZyiGT75. Glycosyltransferase Family 75.

Proteomic databases

PaxDbiQ8H8T0.
PRIDEiQ8H8T0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0599800-01; OS03T0599800-01; OS03G0599800.
GeneIDi4333393.
GrameneiOS03T0599800-01; OS03T0599800-01; OS03G0599800.

Phylogenomic databases

eggNOGiENOG410IHTP. Eukaryota.
ENOG411144T. LUCA.
HOGENOMiHOG000234443.
InParanoidiQ8H8T0.
OMAiIDPYFAK.
OrthoDBiEOG09360C9G.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15774.
BRENDAi5.4.99.30. 4460.

Gene expression databases

ExpressionAtlasiQ8H8T0. baseline and differential.
GenevisibleiQ8H8T0. OS.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR004901. RGP_fam.
[Graphical view]
PfamiPF03214. RGP. 1 hit.
[Graphical view]
PIRSFiPIRSF016429. UPTG. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRGP1_ORYSJ
AccessioniPrimary (citable) accession number: Q8H8T0
Secondary accession number(s): A0A0P0W0V3
, O82705, Q10H67, Q9FUN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.