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Protein

UDP-arabinopyranose mutase 1

Gene

UAM1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf). Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 90:10. Is probably active as heteromer in vivo.4 Publications

Catalytic activityi

UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose.3 Publications

Cofactori

Mn2+1 Publication, Mg2+1 Publication

Kineticsi

  1. KM=22.8 µM for UDP-L-arabinofuranose1 Publication
  2. KM=45.4 µM for UDP-L-arabinopyranose1 Publication
  1. Vmax=0.688 µmol/min/mg enzyme with UDP-L-arabinofuranose as substrate1 Publication
  2. Vmax=0.269 µmol/min/mg enzyme with UDP-L-arabinofuranose as substrate1 Publication

pH dependencei

Optimum pH is 5.5-6.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei158Required for activity1 Publication1
Sitei165Required for activity1 Publication1

GO - Molecular functioni

  • intramolecular transferase activity Source: UniProtKB
  • UDP-arabinopyranose mutase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15774
BRENDAi5.4.99.30 4460

Protein family/group databases

CAZyiGT75 Glycosyltransferase Family 75

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-arabinopyranose mutase 11 Publication (EC:5.4.99.303 Publications)
Short name:
OsUAM11 Publication
Alternative name(s):
Reversibly glycosylated polypeptide 11 Publication
UDP-L-arabinose mutase 1Curated
Gene namesi
Name:UAM11 Publication
Synonyms:RGP11 Publication
Ordered Locus Names:Os03g0599800Imported, LOC_Os03g40270Imported
ORF Names:OJ1523_A02.1Imported, OsJ_11657Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi112D → N: Loss of activity. 1 Publication1
Mutagenesisi151R → A: No effect on the activity. 1 Publication1
Mutagenesisi158R → A: Loss of activity. 1 Publication1
Mutagenesisi165R → A or K: Decreases activity 20-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004109891 – 364UDP-arabinopyranose mutase 1Add BLAST364

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi158N-linked (Glc...) arginine1 Publication1

Post-translational modificationi

Reversibly glycosylated in vitro at Arg-158 by UDP-glucose. Reversibly glycosylated by UDP-xylose and UDP-galactose.3 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8H8T0
PRIDEiQ8H8T0

Expressioni

Gene expression databases

ExpressionAtlasiQ8H8T0 differential
GenevisibleiQ8H8T0 OS

Interactioni

Subunit structurei

Heteromers with UAM2 and UAM3.2 Publications

Protein-protein interaction databases

STRINGi39947.LOC_Os03g40270.1

Structurei

3D structure databases

SMRiQ8H8T0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi110 – 112DXD motif1 Publication3

Domaini

The conserved DXD motif is involved in enzyme activity.1 Publication

Sequence similaritiesi

Belongs to the RGP family.Curated

Phylogenomic databases

eggNOGiENOG410IHTP Eukaryota
ENOG411144T LUCA
HOGENOMiHOG000234443
InParanoidiQ8H8T0
KOiK13379
OMAiTGMVTWI
OrthoDBiEOG09360C9G

Family and domain databases

InterProiView protein in InterPro
IPR029044 Nucleotide-diphossugar_trans
IPR004901 RGP
IPR037595 RGP_fam
PANTHERiPTHR31682 PTHR31682, 1 hit
PfamiView protein in Pfam
PF03214 RGP, 1 hit
PIRSFiPIRSF016429 UPTG, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q8H8T0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTVTVPSA SVPSTPLLKD ELDIVIPTIR NLDFLEMWRP FFQPYHLIIV
60 70 80 90 100
QDGDPTKTIR VPEGFDYELY NRNDINRILG PKASCISFKD SACRCFGYMV
110 120 130 140 150
SKKKYVFTID DDCFVAKDPS GKDINALEQH IKNLLSPSTP FFFNTLYDPY
160 170 180 190 200
REGADFVRGY PFSLREGAKT AVSHGLWLNI PDYDAPTQMV KPRERNSRYV
210 220 230 240 250
DAVMTVPKGT LFPMCGMNLA FDRDLIGPAM YFGLMGDGQP IGRYDDMWAG
260 270 280 290 300
WCMKVICDHL SLGVKTGLPY IWHSKASNPF VNLKKEYKGI FWQEDIIPFF
310 320 330 340 350
QNATIPKECD TVQKCYLSLA EQVREKLGKI DPYFVKLADA MVTWIEAWDE
360
LNPSTAAVEN GKAK
Length:364
Mass (Da):41,349
Last modified:March 1, 2003 - v1
Checksum:i8CB8E232A780934C
GO

Sequence cautioni

The sequence ABF97477 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA09469 differs from that shown. Reason: Frameshift at position 315.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83A → G in CAA09469 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294725 mRNA Translation: AAG17438.1
AJ011078 mRNA Translation: CAA09469.1 Frameshift.
EU267966 mRNA Translation: ACA50488.1
GQ848047 mRNA Translation: ADM86860.1
AC090874 Genomic DNA Translation: AAN08217.1
DP000009 Genomic DNA Translation: ABF97476.1
DP000009 Genomic DNA Translation: ABF97477.1 Different initiation.
AP008209 Genomic DNA Translation: BAF12531.1
AP014959 Genomic DNA Translation: BAS85190.1
CM000140 Genomic DNA Translation: EEE59464.1
AK061813 mRNA Translation: BAG88123.1
AK098933 mRNA Translation: BAG93819.1
RefSeqiXP_015631326.1, XM_015775840.1
UniGeneiOs.5039

Genome annotation databases

EnsemblPlantsiOs03t0599800-01; Os03t0599800-01; Os03g0599800
GeneIDi4333393
GrameneiOs03t0599800-01; Os03t0599800-01; Os03g0599800
KEGGiosa:4333393

Similar proteinsi

Entry informationi

Entry nameiRGP1_ORYSJ
AccessioniPrimary (citable) accession number: Q8H8T0
Secondary accession number(s): A0A0P0W0V3
, O82705, Q10H67, Q9FUN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

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