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Protein

Bifunctional monothiol glutaredoxin-S16, chloroplastic

Gene

GRXS16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May only reduce GSH-thiol disulfides, but not protein disulfides (Probable). Participates probably to the maturation of iron-sulfur proteins and to the regulation of the redox state of the BOLA proteins (Probable). The GRXS16-BOLA1 heterodimer binds a labile, oxygen sensitive iron-sulfur cluster (PubMed:24714563). Able to cleave linearized DNA in vitro (PubMed:23690600).Curated2 Publications

Enzyme regulationi

The formation of an intramolecular disulfide bond negatively regulates both the N-terminal endonuclease and the C-terminal glutaredoxin activities.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei211GlutathioneBy similarity1
Metal bindingi219Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Binding sitei251GlutathioneBy similarity1
Binding sitei263Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cation transport Source: TAIR
  • cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional monothiol glutaredoxin-S16, chloroplastic1 Publication
Short name:
AtGrxS161 Publication
Alternative name(s):
Atypical GIY-YIG endonuclease1 Publication (EC:3.1.-.-)
CAX-interacting protein 21 Publication
CAXIP1-like protein1 Publication
Gene namesi
Name:GRXS161 Publication
Synonyms:CXIP21 Publication
Ordered Locus Names:At2g38270Imported
ORF Names:F16M14.20Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G38270.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi90Y → F: Strongly reduced nuclease activity. 1 Publication1
Mutagenesisi101F → Y: Enhanced nuclease activity. 1 Publication1
Mutagenesisi111S → A: Strongly reduced nuclease activity. 1 Publication1
Mutagenesisi111S → R: Slightly reduced nuclease activity. 1 Publication1
Mutagenesisi115H → F: Strongly reduced nuclease activity. 1 Publication1
Mutagenesisi123C → S: Increased glutaredoxin activity. 1 Publication1
Mutagenesisi143W → A or E: Slightly reduced nuclease activity. 1 Publication1
Mutagenesisi161N → L: Slightly reduced nuclease activity. 1 Publication1
Mutagenesisi219C → S: Decreased interactions with BOLA proteins and loss of interaction with SUFE1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 62ChloroplastSequence analysisAdd BLAST62
ChainiPRO_000026873663 – 293Bifunctional monothiol glutaredoxin-S16, chloroplasticAdd BLAST231

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi123 ↔ 2191 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8H7F6.
PRIDEiQ8H7F6.

Expressioni

Gene expression databases

GenevisibleiQ8H7F6. AT.

Interactioni

Subunit structurei

[2Fe-2S]-bridged holo-homodimer (PubMed:23690600). Interacts in vitro with SUFE1, BOLA1, BOLA2 and BOLA4 (PubMed:24203231). Interacts in vivo only with SUFE1, BOLA1 and BOLA4 (PubMed:24203231, PubMed:24714563).3 Publications

Protein-protein interaction databases

BioGridi3749. 12 interactors.
IntActiQ8H7F6. 7 interactors.
STRINGi3702.AT2G38270.1.

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi69 – 71Combined sources3
Beta strandi73 – 77Combined sources5
Helixi80 – 82Combined sources3
Beta strandi86 – 93Combined sources8
Beta strandi99 – 106Combined sources8
Helixi108 – 118Combined sources11
Helixi120 – 122Combined sources3
Beta strandi124 – 130Combined sources7
Helixi136 – 153Combined sources18
Turni159 – 163Combined sources5
Turni167 – 169Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LWFNMR-A63-170[»]
ProteinModelPortaliQ8H7F6.
SMRiQ8H7F6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini194 – 293GlutaredoxinPROSITE-ProRule annotationAdd BLAST100

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni276 – 277Glutathione bindingBy similarity2

Domaini

The N-terminal domain (NTD) (63-170) has an intrinsic Mg2+-dependent endonuclease activity in vitro (PubMed:23690600). The glutaredoxin (GRX) domain (194-293) alone is able to complement yeast grx5 cells in vitro, but not the full length GRXS16 protein.1 Publication

Sequence similaritiesi

Belongs to the glutaredoxin family. CGFS subfamily.Curated
Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0911. Eukaryota.
COG0278. LUCA.
HOGENOMiHOG000243994.
InParanoidiQ8H7F6.
OMAiTSMHEKG.
OrthoDBiEOG09360HE3.
PhylomeDBiQ8H7F6.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT-like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8H7F6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAITISSSL HASASPRVVR PHVSRNTPVI TLYSRFTPSF SFPSLSFTLR
60 70 80 90 100
DTAPSRRRSF FIASAVKSLT ETELLPITEA DSIPSASGVY AVYDKSDELQ
110 120 130 140 150
FVGISRNIAA SVSAHLKSVP ELCGSVKVGI VEEPDKAVLT QAWKLWIEEH
160 170 180 190 200
IKVTGKVPPG NKSGNNTFVK QTPRKKSDIR LTPGRHVELT VPLEELIDRL
210 220 230 240 250
VKESKVVAFI KGSRSAPQCG FSQRVVGILE SQGVDYETVD VLDDEYNHGL
260 270 280 290
RETLKNYSNW PTFPQIFVKG ELVGGCDILT SMYENGELAN ILN
Length:293
Mass (Da):32,206
Last modified:December 12, 2006 - v2
Checksum:i6B2E6337F8C5F050
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194E → G in AAN60257 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157989 mRNA. Translation: AAO19648.1.
AF083698 mRNA. Translation: AAN60257.1.
AC003028 Genomic DNA. Translation: AAC27175.1.
CP002685 Genomic DNA. Translation: AEC09515.1.
AK117441 mRNA. Translation: BAC42106.1.
BT004974 mRNA. Translation: AAO50507.1.
AY086273 mRNA. Translation: AAM64346.1.
PIRiT01258.
RefSeqiNP_565885.1. NM_129383.3.
UniGeneiAt.25559.
At.71438.

Genome annotation databases

EnsemblPlantsiAT2G38270.1; AT2G38270.1; AT2G38270.
GeneIDi818407.
GrameneiAT2G38270.1; AT2G38270.1; AT2G38270.
KEGGiath:AT2G38270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157989 mRNA. Translation: AAO19648.1.
AF083698 mRNA. Translation: AAN60257.1.
AC003028 Genomic DNA. Translation: AAC27175.1.
CP002685 Genomic DNA. Translation: AEC09515.1.
AK117441 mRNA. Translation: BAC42106.1.
BT004974 mRNA. Translation: AAO50507.1.
AY086273 mRNA. Translation: AAM64346.1.
PIRiT01258.
RefSeqiNP_565885.1. NM_129383.3.
UniGeneiAt.25559.
At.71438.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LWFNMR-A63-170[»]
ProteinModelPortaliQ8H7F6.
SMRiQ8H7F6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3749. 12 interactors.
IntActiQ8H7F6. 7 interactors.
STRINGi3702.AT2G38270.1.

Proteomic databases

PaxDbiQ8H7F6.
PRIDEiQ8H7F6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G38270.1; AT2G38270.1; AT2G38270.
GeneIDi818407.
GrameneiAT2G38270.1; AT2G38270.1; AT2G38270.
KEGGiath:AT2G38270.

Organism-specific databases

TAIRiAT2G38270.

Phylogenomic databases

eggNOGiKOG0911. Eukaryota.
COG0278. LUCA.
HOGENOMiHOG000243994.
InParanoidiQ8H7F6.
OMAiTSMHEKG.
OrthoDBiEOG09360HE3.
PhylomeDBiQ8H7F6.

Miscellaneous databases

PROiQ8H7F6.

Gene expression databases

GenevisibleiQ8H7F6. AT.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT-like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRS16_ARATH
AccessioniPrimary (citable) accession number: Q8H7F6
Secondary accession number(s): O80451, Q8LD13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: November 30, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.