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Q8H7F6

- GRS16_ARATH

UniProt

Q8H7F6 - GRS16_ARATH

Protein

Monothiol glutaredoxin-S16, chloroplastic

Gene

GRXS16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    May only reduce GSH-thiol disulfides, but not protein disulfides.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei211 – 2111GlutathioneBy similarity
    Metal bindingi219 – 2191Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity
    Binding sitei251 – 2511GlutathioneBy similarity
    Binding sitei263 – 2631Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. protein disulfide oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. cation transport Source: TAIR
    2. cell redox homeostasis Source: InterPro

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monothiol glutaredoxin-S16, chloroplastic
    Short name:
    AtGrxS16
    Alternative name(s):
    CAXIP1-like protein
    Gene namesi
    Name:GRXS16
    Ordered Locus Names:At2g38270
    ORF Names:F16M14.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G38270.

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6262ChloroplastSequence AnalysisAdd
    BLAST
    Chaini63 – 293231Monothiol glutaredoxin-S16, chloroplasticPRO_0000268736Add
    BLAST

    Proteomic databases

    PaxDbiQ8H7F6.
    PRIDEiQ8H7F6.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8H7F6.

    Interactioni

    Protein-protein interaction databases

    BioGridi3749. 12 interactions.
    IntActiQ8H7F6. 7 interactions.
    STRINGi3702.AT2G38270.1-P.

    Structurei

    Secondary structure

    1
    293
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi69 – 713
    Beta strandi73 – 775
    Helixi80 – 823
    Beta strandi86 – 938
    Beta strandi99 – 1068
    Helixi108 – 11811
    Helixi120 – 1223
    Beta strandi124 – 1307
    Helixi136 – 15318
    Turni159 – 1635
    Turni167 – 1693

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LWFNMR-A63-170[»]
    ProteinModelPortaliQ8H7F6.
    SMRiQ8H7F6. Positions 62-172, 195-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini194 – 293100GlutaredoxinPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni276 – 2772Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the glutaredoxin family. CGFS subfamily.Curated
    Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0278.
    HOGENOMiHOG000243994.
    InParanoidiQ8H7F6.
    OMAiYNYGLRE.
    PhylomeDBiQ8H7F6.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR002109. Glutaredoxin.
    IPR004480. Monothiol_GRX-rel.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR10293. PTHR10293. 1 hit.
    PfamiPF00462. Glutaredoxin. 1 hit.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
    PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8H7F6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAITISSSL HASASPRVVR PHVSRNTPVI TLYSRFTPSF SFPSLSFTLR    50
    DTAPSRRRSF FIASAVKSLT ETELLPITEA DSIPSASGVY AVYDKSDELQ 100
    FVGISRNIAA SVSAHLKSVP ELCGSVKVGI VEEPDKAVLT QAWKLWIEEH 150
    IKVTGKVPPG NKSGNNTFVK QTPRKKSDIR LTPGRHVELT VPLEELIDRL 200
    VKESKVVAFI KGSRSAPQCG FSQRVVGILE SQGVDYETVD VLDDEYNHGL 250
    RETLKNYSNW PTFPQIFVKG ELVGGCDILT SMYENGELAN ILN 293
    Length:293
    Mass (Da):32,206
    Last modified:December 12, 2006 - v2
    Checksum:i6B2E6337F8C5F050
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti194 – 1941E → G in AAN60257. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY157989 mRNA. Translation: AAO19648.1.
    AF083698 mRNA. Translation: AAN60257.1.
    AC003028 Genomic DNA. Translation: AAC27175.1.
    CP002685 Genomic DNA. Translation: AEC09515.1.
    AK117441 mRNA. Translation: BAC42106.1.
    BT004974 mRNA. Translation: AAO50507.1.
    AY086273 mRNA. Translation: AAM64346.1.
    PIRiT01258.
    RefSeqiNP_565885.1. NM_129383.2.
    UniGeneiAt.25559.
    At.71438.

    Genome annotation databases

    EnsemblPlantsiAT2G38270.1; AT2G38270.1; AT2G38270.
    GeneIDi818407.
    KEGGiath:AT2G38270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY157989 mRNA. Translation: AAO19648.1 .
    AF083698 mRNA. Translation: AAN60257.1 .
    AC003028 Genomic DNA. Translation: AAC27175.1 .
    CP002685 Genomic DNA. Translation: AEC09515.1 .
    AK117441 mRNA. Translation: BAC42106.1 .
    BT004974 mRNA. Translation: AAO50507.1 .
    AY086273 mRNA. Translation: AAM64346.1 .
    PIRi T01258.
    RefSeqi NP_565885.1. NM_129383.2.
    UniGenei At.25559.
    At.71438.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LWF NMR - A 63-170 [» ]
    ProteinModelPortali Q8H7F6.
    SMRi Q8H7F6. Positions 62-172, 195-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 3749. 12 interactions.
    IntActi Q8H7F6. 7 interactions.
    STRINGi 3702.AT2G38270.1-P.

    Proteomic databases

    PaxDbi Q8H7F6.
    PRIDEi Q8H7F6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G38270.1 ; AT2G38270.1 ; AT2G38270 .
    GeneIDi 818407.
    KEGGi ath:AT2G38270.

    Organism-specific databases

    TAIRi AT2G38270.

    Phylogenomic databases

    eggNOGi COG0278.
    HOGENOMi HOG000243994.
    InParanoidi Q8H7F6.
    OMAi YNYGLRE.
    PhylomeDBi Q8H7F6.

    Gene expression databases

    Genevestigatori Q8H7F6.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR002109. Glutaredoxin.
    IPR004480. Monothiol_GRX-rel.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    PANTHERi PTHR10293. PTHR10293. 1 hit.
    Pfami PF00462. Glutaredoxin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR00365. TIGR00365. 1 hit.
    PROSITEi PS51354. GLUTAREDOXIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of CXIP1 A novel PICOT domain-containing Arabidopsis protein that associates with CAX1."
      Cheng N.-H., Hirschi K.D.
      J. Biol. Chem. 278:6503-6509(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells."
      Stracke R., Palme K.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. "Plant glutaredoxins: still mysterious reducing systems."
      Rouhier N., Gelhaye E., Jacquot J.-P.
      Cell. Mol. Life Sci. 61:1266-1277(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    9. "Genome-wide analysis of plant glutaredoxin systems."
      Rouhier N., Couturier J., Jacquot J.-P.
      J. Exp. Bot. 57:1685-1696(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.

    Entry informationi

    Entry nameiGRS16_ARATH
    AccessioniPrimary (citable) accession number: Q8H7F6
    Secondary accession number(s): O80451, Q8LD13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3