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Protein

Floral defensin-like protein 1

Gene

D1

Organism
Petunia hybrida (Petunia)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea.

pH dependencei

Stable under extremes of pH.

Temperature dependencei

Stable under extremes of temperature.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Floral defensin-like protein 1
Alternative name(s):
PhD1
Gene namesi
Name:D1
OrganismiPetunia hybrida (Petunia)
Taxonomic identifieri4102 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000000704726 – 72Floral defensin-like protein 1Add BLAST47
PropeptideiPRO_000000704873 – 103Removed in mature formAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 72
Disulfide bondi32 ↔ 48
Disulfide bondi39 ↔ 59
Disulfide bondi45 ↔ 66
Disulfide bondi49 ↔ 68

Post-translational modificationi

When compared to other plant defensins, the petunia defensins have an additional fifth disulfide bond.

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Petals.

Structurei

Secondary structure

1103
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 31Combined sources4
Helixi42 – 51Combined sources10
Beta strandi55 – 59Combined sources5
Beta strandi61 – 63Combined sources3
Beta strandi66 – 70Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N4NNMR-A26-72[»]
ProteinModelPortaliQ8H6Q1.
SMRiQ8H6Q1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8H6Q1.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the DEFL family.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8H6Q1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSICFFAV AILALMLFAA YDAEAATCKA ECPTWDSVCI NKKPCVACCK
60 70 80 90 100
KAKFSDGHCS KILRRCLCTK ECVFEKTEAT QTETFTKDVN TLAEALLEAD

MMV
Length:103
Mass (Da):11,361
Last modified:March 1, 2003 - v1
Checksum:iA8B629A3E06A0D01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF507975 mRNA. Translation: AAN64750.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF507975 mRNA. Translation: AAN64750.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N4NNMR-A26-72[»]
ProteinModelPortaliQ8H6Q1.
SMRiQ8H6Q1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ8H6Q1.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDEF1_PETHY
AccessioniPrimary (citable) accession number: Q8H6Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.