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Q8H4L3 (CHS2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chalcone synthase 2

Short name=OsCHS2
EC=2.3.1.74
Alternative name(s):
Naregenin-chalcone synthase
Gene names
Name:CHS2
Ordered Locus Names:Os07g0214900, LOC_Os07g11440
ORF Names:OJ1116_C08.125
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin By similarity.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
   Biological processFlavonoid biosynthesis
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processflavonoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionnaringenin-chalcone synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403Chalcone synthase 2
PRO_0000407327

Regions

Region59 – 668Coenzyme A binding By similarity
Region220 – 2212Substrate binding By similarity

Sites

Active site1681Acyl-thioester intermediate By similarity
Binding site3131Coenzyme A; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8H4L3 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 1E2622C4769F7B62

FASTA40343,925
        10         20         30         40         50         60 
MVTSTVKLEE VRRMQRAEGM AAVLAIGTAT PANCVYQTDY PDYYFRVTNS EHLTNLKERF 

        70         80         90        100        110        120 
QRMCESSQIR KRYTHLTEEI LQENPSMCVF TAPSLDARQD MVVAEVPKLG KAAAEEAIKE 

       130        140        150        160        170        180 
WGQPMSRITH LVFCTTNGVD MPGADYQVAK MLGLPTSVKR LMMYQQGCFA GGTVLRVAKD 

       190        200        210        220        230        240 
LAENNRGARV LVVCSEIMAM AFRGPSESHL DSLVGHALFG DGAAAVIVGS DPDEAADERP 

       250        260        270        280        290        300 
LFQIVSASQT ILPGTEDAIV GHLREVGLTF HLPKDVPEFI SDSVEGALTD AFMPLGVHDW 

       310        320        330        340        350        360 
NSIFWVVHPG GPAILDQVEE KVALHKARMR ASRNVLSEYG NMASATVLFV LDEMRKLSAD 

       370        380        390        400 
DGHATTGEGM DWGVLFGFGP GLTVETIVLH SVPITAAAPL IMQ 

« Hide

References

« Hide 'large scale' references
[1]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP004002 Genomic DNA. Translation: BAC20662.1.
AP008213 Genomic DNA. Translation: BAF21101.1.
RefSeqNP_001059187.1. NM_001065722.1.
UniGeneOs.78839.

3D structure databases

ProteinModelPortalQ8H4L3.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ8H4L3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS07T0214900-00; OS07T0214900-00; OS07G0214900.
GeneID4342725.
KEGGosa:4342725.

Organism-specific databases

GrameneQ8H4L3.

Phylogenomic databases

eggNOGCOG3424.

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHS2_ORYSJ
AccessionPrimary (citable) accession number: Q8H4L3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: March 1, 2003
Last modified: December 11, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways