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Protein

Chalcone synthase 2

Gene

CHS2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.By similarity

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Acyl-thioester intermediatePROSITE-ProRule annotation
Binding sitei313 – 3131Coenzyme A; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase 2 (EC:2.3.1.74)
Short name:
OsCHS2
Alternative name(s):
Naregenin-chalcone synthase
Gene namesi
Name:CHS2
Ordered Locus Names:Os07g0214900, LOC_Os07g11440
ORF Names:OJ1116_C08.125
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 7

Organism-specific databases

GrameneiQ8H4L3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Chalcone synthase 2PRO_0000407327Add
BLAST

Proteomic databases

PRIDEiQ8H4L3.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8H4L3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni59 – 668Coenzyme A bindingBy similarity
Regioni220 – 2212Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Phylogenomic databases

eggNOGiCOG3424.
InParanoidiQ8H4L3.
OMAiLCVELTS.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8H4L3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTSTVKLEE VRRMQRAEGM AAVLAIGTAT PANCVYQTDY PDYYFRVTNS
60 70 80 90 100
EHLTNLKERF QRMCESSQIR KRYTHLTEEI LQENPSMCVF TAPSLDARQD
110 120 130 140 150
MVVAEVPKLG KAAAEEAIKE WGQPMSRITH LVFCTTNGVD MPGADYQVAK
160 170 180 190 200
MLGLPTSVKR LMMYQQGCFA GGTVLRVAKD LAENNRGARV LVVCSEIMAM
210 220 230 240 250
AFRGPSESHL DSLVGHALFG DGAAAVIVGS DPDEAADERP LFQIVSASQT
260 270 280 290 300
ILPGTEDAIV GHLREVGLTF HLPKDVPEFI SDSVEGALTD AFMPLGVHDW
310 320 330 340 350
NSIFWVVHPG GPAILDQVEE KVALHKARMR ASRNVLSEYG NMASATVLFV
360 370 380 390 400
LDEMRKLSAD DGHATTGEGM DWGVLFGFGP GLTVETIVLH SVPITAAAPL

IMQ
Length:403
Mass (Da):43,925
Last modified:March 1, 2003 - v1
Checksum:i1E2622C4769F7B62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004002 Genomic DNA. Translation: BAC20662.1.
AP008213 Genomic DNA. Translation: BAF21101.1.
RefSeqiNP_001059187.1. NM_001065722.1.
UniGeneiOs.78839.

Genome annotation databases

EnsemblPlantsiOS07T0214900-00; OS07T0214900-00; OS07G0214900.
GeneIDi4342725.
KEGGiosa:4342725.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004002 Genomic DNA. Translation: BAC20662.1.
AP008213 Genomic DNA. Translation: BAF21101.1.
RefSeqiNP_001059187.1. NM_001065722.1.
UniGeneiOs.78839.

3D structure databases

ProteinModelPortaliQ8H4L3.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ8H4L3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS07T0214900-00; OS07T0214900-00; OS07G0214900.
GeneIDi4342725.
KEGGiosa:4342725.

Organism-specific databases

GrameneiQ8H4L3.

Phylogenomic databases

eggNOGiCOG3424.
InParanoidiQ8H4L3.
OMAiLCVELTS.

Enzyme and pathway databases

UniPathwayiUPA00154.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiCHS2_ORYSJ
AccessioniPrimary (citable) accession number: Q8H4L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.