Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8H3I4

- GLO4_ORYSJ

UniProt

Q8H3I4 - GLO4_ORYSJ

Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO4

Gene

GLO4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (01 Oct 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Photorespiratory enzyme that can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase. Not required for oxalate accumulation By similarity.By similarity

    Catalytic activityi

    (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

    Cofactori

    FMN.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei27 – 271SubstratePROSITE-ProRule annotation
    Binding sitei109 – 1091FMNPROSITE-ProRule annotation
    Binding sitei130 – 1301FMNPROSITE-ProRule annotation
    Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
    Binding sitei158 – 1581FMNPROSITE-ProRule annotation
    Binding sitei167 – 1671SubstratePROSITE-ProRule annotation
    Binding sitei231 – 2311FMNPROSITE-ProRule annotation
    Active sitei255 – 2551Proton acceptorPROSITE-ProRule annotation
    Binding sitei258 – 2581SubstratePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi286 – 31025FMNPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. glycolate oxidase activity Source: UniProtKB
    3. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
    4. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
    5. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response signaling pathway, resistance gene-independent Source: EnsemblPlants/Gramene
    2. defense response to bacterium Source: EnsemblPlants/Gramene
    3. hydrogen peroxide biosynthetic process Source: EnsemblPlants/Gramene
    4. modulation by virus of host morphology or physiology Source: UniProtKB
    5. oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
    6. photorespiration Source: UniProtKB
    7. regulation of photosynthesis Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolate pathway, Host-virus interaction, Photorespiration

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    UniPathwayiUPA00951; UER00912.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (EC:1.1.3.15)
    Alternative name(s):
    Glycolate oxidase 4
    Short name:
    GOX 4
    Short name:
    OsGLO4
    Short chain alpha-hydroxy acid oxidase GLO4
    Gene namesi
    Name:GLO4
    Ordered Locus Names:Os07g0616500
    ORF Names:B1056G08.112, OsJ_25131
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 7

    Organism-specific databases

    GrameneiQ8H3I4.

    Subcellular locationi

    Peroxisome By similarity

    GO - Cellular componenti

    1. peroxisome Source: UniProtKB

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366Peroxisomal (S)-2-hydroxy-acid oxidase GLO4PRO_0000403415Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer or homooctamer. Interacts with rice dwarf virus (RDV) P8. This interaction promotes viral P8 relocation to virus factories peripheral to peroxisomes By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H3I4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 360360FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi364 – 3663Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
    Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1304.
    KOiK11517.
    OMAiAKAVMIG.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view]
    PfamiPF01070. FMN_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8H3I4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDNLPVNVR EYQELAKKAL PKMAYDYING GAEDEHTLRE NIAAYTRIIL    50
    RPRVLVDVSK IDMSTTLLGY TMRSPIIVAP TGGHKLAHPE GEKATARAAA 100
    SCNAIMVLSF SSSCKIEDVA SSCNAIRFYQ LYVYKNRNVS ATLVRRAESC 150
    GFKALLLTVD TPMLGRREAD IRNKMVFPRS GNLEGLMTTD DHDTTNGSQL 200
    ERFARATLDP SLSWKDIEWL KSITSMPIFL KGIVTAEDAR RAVEAGVAGV 250
    IVSNHGARQL DYAPATIAAL EEVVRAVAGA VPVLVDGGIR RGTDVFKALA 300
    LGARAVMVGR PVFFGLAARG EAGARHVIEM LNGELEVAMA LCGCRSVGEI 350
    TRSHVMTEGD RIRSLL 366
    Length:366
    Mass (Da):39,764
    Last modified:October 1, 2003 - v2
    Checksum:i73FCBAD72CC9B9F5
    GO

    Sequence cautioni

    The sequence EEE67592.1 differs from that shown. Reason: Frameshift at position 321.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti313 – 3131F → L in EEE67592. (PubMed:15685292)Curated
    Sequence conflicti321 – 3211E → D in EEE67592. (PubMed:15685292)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP004988 Genomic DNA. Translation: BAC79990.1.
    AP008213 Genomic DNA. Translation: BAF22190.1.
    CM000144 Genomic DNA. Translation: EEE67592.1. Frameshift.
    AK071738 mRNA. Translation: BAG92663.1.
    RefSeqiNP_001060276.1. NM_001066811.1.
    UniGeneiOs.8126.

    Genome annotation databases

    EnsemblPlantsiOS07T0616500-01; OS07T0616500-01; OS07G0616500.
    GeneIDi4343908.
    KEGGiosa:4343908.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP004988 Genomic DNA. Translation: BAC79990.1 .
    AP008213 Genomic DNA. Translation: BAF22190.1 .
    CM000144 Genomic DNA. Translation: EEE67592.1 . Frameshift.
    AK071738 mRNA. Translation: BAG92663.1 .
    RefSeqi NP_001060276.1. NM_001066811.1.
    UniGenei Os.8126.

    3D structure databases

    ProteinModelPortali Q8H3I4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS07T0616500-01 ; OS07T0616500-01 ; OS07G0616500 .
    GeneIDi 4343908.
    KEGGi osa:4343908.

    Organism-specific databases

    Gramenei Q8H3I4.

    Phylogenomic databases

    eggNOGi COG1304.
    KOi K11517.
    OMAi AKAVMIG.

    Enzyme and pathway databases

    UniPathwayi UPA00951 ; UER00912 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view ]
    Pfami PF01070. FMN_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    3. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    5. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
      Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
      J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiGLO4_ORYSJ
    AccessioniPrimary (citable) accession number: Q8H3I4
    Secondary accession number(s): B9FU85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3