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Q8H3I4

- GLO4_ORYSJ

UniProt

Q8H3I4 - GLO4_ORYSJ

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Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO4

Gene

GLO4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Photorespiratory enzyme that can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase. Not required for oxalate accumulation (By similarity).By similarity

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMN.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271SubstratePROSITE-ProRule annotation
Binding sitei109 – 1091FMNPROSITE-ProRule annotation
Binding sitei130 – 1301FMNPROSITE-ProRule annotation
Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
Binding sitei158 – 1581FMNPROSITE-ProRule annotation
Binding sitei167 – 1671SubstratePROSITE-ProRule annotation
Binding sitei231 – 2311FMNPROSITE-ProRule annotation
Active sitei255 – 2551Proton acceptorPROSITE-ProRule annotation
Binding sitei258 – 2581SubstratePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi286 – 31025FMNPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. glycolate oxidase activity Source: UniProtKB
  3. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
  4. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
  5. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. modulation by virus of host morphology or physiology Source: UniProtKB
  2. oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
  3. photorespiration Source: UniProtKB
  4. regulation of photosynthesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolate pathway, Host-virus interaction, Photorespiration

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA00951; UER00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 4
Short name:
GOX 4
Short name:
OsGLO4
Short chain alpha-hydroxy acid oxidase GLO4
Gene namesi
Name:GLO4
Ordered Locus Names:Os07g0616500
ORF Names:B1056G08.112, OsJ_25131
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 7

Organism-specific databases

GrameneiQ8H3I4.

Subcellular locationi

Peroxisome By similarity

GO - Cellular componenti

  1. peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Peroxisomal (S)-2-hydroxy-acid oxidase GLO4PRO_0000403415Add
BLAST

Interactioni

Subunit structurei

Homotetramer or homooctamer. Interacts with rice dwarf virus (RDV) P8. This interaction promotes viral P8 relocation to virus factories peripheral to peroxisomes (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8H3I4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 360360FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi364 – 3663Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1304.
InParanoidiQ8H3I4.
KOiK11517.
OMAiAKAVMIG.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8H3I4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDNLPVNVR EYQELAKKAL PKMAYDYING GAEDEHTLRE NIAAYTRIIL
60 70 80 90 100
RPRVLVDVSK IDMSTTLLGY TMRSPIIVAP TGGHKLAHPE GEKATARAAA
110 120 130 140 150
SCNAIMVLSF SSSCKIEDVA SSCNAIRFYQ LYVYKNRNVS ATLVRRAESC
160 170 180 190 200
GFKALLLTVD TPMLGRREAD IRNKMVFPRS GNLEGLMTTD DHDTTNGSQL
210 220 230 240 250
ERFARATLDP SLSWKDIEWL KSITSMPIFL KGIVTAEDAR RAVEAGVAGV
260 270 280 290 300
IVSNHGARQL DYAPATIAAL EEVVRAVAGA VPVLVDGGIR RGTDVFKALA
310 320 330 340 350
LGARAVMVGR PVFFGLAARG EAGARHVIEM LNGELEVAMA LCGCRSVGEI
360
TRSHVMTEGD RIRSLL
Length:366
Mass (Da):39,764
Last modified:October 1, 2003 - v2
Checksum:i73FCBAD72CC9B9F5
GO

Sequence cautioni

The sequence EEE67592.1 differs from that shown. Reason: Frameshift at position 321.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti313 – 3131F → L in EEE67592. (PubMed:15685292)Curated
Sequence conflicti321 – 3211E → D in EEE67592. (PubMed:15685292)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP004988 Genomic DNA. Translation: BAC79990.1.
AP008213 Genomic DNA. Translation: BAF22190.1.
CM000144 Genomic DNA. Translation: EEE67592.1. Frameshift.
AK071738 mRNA. Translation: BAG92663.1.
RefSeqiNP_001060276.1. NM_001066811.1.
UniGeneiOs.8126.

Genome annotation databases

EnsemblPlantsiOS07T0616500-01; OS07T0616500-01; OS07G0616500.
GeneIDi4343908.
KEGGiosa:4343908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP004988 Genomic DNA. Translation: BAC79990.1 .
AP008213 Genomic DNA. Translation: BAF22190.1 .
CM000144 Genomic DNA. Translation: EEE67592.1 . Frameshift.
AK071738 mRNA. Translation: BAG92663.1 .
RefSeqi NP_001060276.1. NM_001066811.1.
UniGenei Os.8126.

3D structure databases

ProteinModelPortali Q8H3I4.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS07T0616500-01 ; OS07T0616500-01 ; OS07G0616500 .
GeneIDi 4343908.
KEGGi osa:4343908.

Organism-specific databases

Gramenei Q8H3I4.

Phylogenomic databases

eggNOGi COG1304.
InParanoidi Q8H3I4.
KOi K11517.
OMAi AKAVMIG.

Enzyme and pathway databases

UniPathwayi UPA00951 ; UER00912 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view ]
Pfami PF01070. FMN_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  5. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
    Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
    J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiGLO4_ORYSJ
AccessioniPrimary (citable) accession number: Q8H3I4
Secondary accession number(s): B9FU85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: October 1, 2003
Last modified: October 29, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3