Glycerol-3-phosphate dehydrogenase [NAD(+)], chloroplastic precursor

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Q8H2J9 (GPDA_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], chloroplastic
EC=1.1.1.8

Gene names

Ordered Locus Names:	Os07g0229800, LOC_Os07g12640
ORF Names:	OSJNBa0016A21.127

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required to supply glycerol-3-phosphate in the chloroplast for the synthesis of glycerolipids By similarity.
Catalytic activity	sn-glycerol 3-phosphate + NAD⁺ = glycerone phosphate + NADH.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.
Sequence caution	The sequence BAC84439.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF21142.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAG89576.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro glycerolipid biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene glycerophospholipid metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW systemic acquired resistance Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase [NAD+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 47

Chloroplast Potential

Chain

48 – 440

393

Glycerol-3-phosphate dehydrogenase [NAD(+)], chloroplastic

PRO_0000420175

Regions

Nucleotide binding

114 – 119

NAD By similarity

Region

363 – 364

Substrate binding By similarity

Sites

Active site

299

Proton acceptor By similarity

Binding site

191

NAD By similarity

Binding site

214

NAD; via amide nitrogen By similarity

Binding site

214

Substrate By similarity

Binding site

248

NAD; via amide nitrogen By similarity

Binding site

363

NAD By similarity

Binding site

389

NAD By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8H2J9 [UniParc].

Last modified November 28, 2012. Version 3.
Checksum: 5A1D054CC6312A30
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FASTA

440

46,762

        10         20         30         40         50         60 
MAAAAAATFL PHTPTPRRRL AVAVHSPTRR RLSLVFSGPP DGALSVAAAE EKADAGEEAA 

        70         80         90        100        110        120 
AAVSAPRGGG GGGGKERRRV VRKAWEKLVR WSRSWRRRNR SDVVETTRKV VVLGGGSFGT 

       130        140        150        160        170        180 
AMAAQVAAKK ADLEVSMLLR DDLVCRSINH SHINCKYLRD HRLPENITAT TSASDALAGA 

       190        200        210        220        230        240 
DFCFHAVPVQ FSSSFLEGIS THVDPKLPFI SLSKGLELNT LRTMSQIIPQ ALGNPRQPFI 

       250        260        270        280        290        300 
VLSGPSFAIE LMNKLPTAMV VASKDKKLAA AVQQLLASPN LRISTSNDVT GVEIAGALKN 

       310        320        330        340        350        360 
VLAIAAGIVE GMHLGNNCMA ALVAQGCSEI RWLATKMGAK PTTLSGLSGS GDIMLTCFVN 

       370        380        390        400        410        420 
LSRNRNVGLR LGSGEKLDEI MNSMNQVAEG VSTAGAVIAL AQKYHVKMPV LTAVARIIDN 

       430        440 
ELTPKKAVME LMNLPQVEEV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP005764 Genomic DNA. Translation: BAC84439.1. Sequence problems. AP008213 Genomic DNA. Translation: BAF21142.1. Sequence problems. AK065591 mRNA. Translation: BAG89576.1. Sequence problems.
RefSeq	NP_001059228.1. NM_001065763.1.
UniGene	Os.18760.
3D structure databases
HSSP	HSSP built from PDB template 1EVY based on UniProtKB P90551.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os07g12640.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os07g12640.1; LOC_Os07g12640.1; LOC_Os07g12640.
GeneID	4342770.
KEGG	osa:4342770.
Organism-specific databases
Gramene	Q8H2J9.
Phylogenomic databases
HOGENOM	HOG000246853.
ProtClustDB	CLSN2688847.
Enzyme and pathway databases
UniPathway	UPA00940.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR006168. G3P_DH_NAD-dep. IPR006109. G3P_DH_NAD-dep_C. IPR011128. G3P_DH_NAD-dep_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11728. PTHR11728. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PRINTS	PR00077. GPDHDRGNASE.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDA_ORYSJ

Accession

Primary (citable) accession number: Q8H2J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2012
Last sequence update:	November 28, 2012
Last modified:	April 3, 2013
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents