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Q8H2J9

- GPDA_ORYSJ

UniProt

Q8H2J9 - GPDA_ORYSJ

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], chloroplastic

Gene

Os07g0229800

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 3 (28 Nov 2012)
      Previous versions | rss
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    Functioni

    Required to supply glycerol-3-phosphate in the chloroplast for the synthesis of glycerolipids.By similarity

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei191 – 1911NADBy similarity
    Binding sitei214 – 2141NAD; via amide nitrogenBy similarity
    Binding sitei214 – 2141SubstrateBy similarity
    Binding sitei248 – 2481NAD; via amide nitrogenBy similarity
    Active sitei299 – 2991Proton acceptorBy similarity
    Binding sitei363 – 3631NADBy similarity
    Binding sitei389 – 3891NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi114 – 1196NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
    2. NAD binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. glycerol-3-phosphate catabolic process Source: InterPro
    3. glycerolipid biosynthetic process Source: EnsemblPlants/Gramene
    4. glycerophospholipid metabolic process Source: UniProtKB-UniPathway
    5. phospholipid biosynthetic process Source: UniProtKB-KW
    6. systemic acquired resistance Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00940.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)], chloroplastic (EC:1.1.1.8)
    Gene namesi
    Ordered Locus Names:Os07g0229800, LOC_Os07g12640
    ORF Names:OSJNBa0016A21.127
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 7

    Organism-specific databases

    GrameneiQ8H2J9.

    Subcellular locationi

    Plastidchloroplast By similarity

    GO - Cellular componenti

    1. chloroplast envelope Source: EnsemblPlants/Gramene
    2. glycerol-3-phosphate dehydrogenase complex Source: InterPro

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4747ChloroplastSequence AnalysisAdd
    BLAST
    Chaini48 – 440393Glycerol-3-phosphate dehydrogenase [NAD(+)], chloroplasticPRO_0000420175Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os07g12640.1.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni363 – 3642Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000246853.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_00394. NAD_Glyc3P_dehydrog.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    PROSITEiPS00957. NAD_G3PDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8H2J9-1 [UniParc]FASTAAdd to Basket

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    MAAAAAATFL PHTPTPRRRL AVAVHSPTRR RLSLVFSGPP DGALSVAAAE    50
    EKADAGEEAA AAVSAPRGGG GGGGKERRRV VRKAWEKLVR WSRSWRRRNR 100
    SDVVETTRKV VVLGGGSFGT AMAAQVAAKK ADLEVSMLLR DDLVCRSINH 150
    SHINCKYLRD HRLPENITAT TSASDALAGA DFCFHAVPVQ FSSSFLEGIS 200
    THVDPKLPFI SLSKGLELNT LRTMSQIIPQ ALGNPRQPFI VLSGPSFAIE 250
    LMNKLPTAMV VASKDKKLAA AVQQLLASPN LRISTSNDVT GVEIAGALKN 300
    VLAIAAGIVE GMHLGNNCMA ALVAQGCSEI RWLATKMGAK PTTLSGLSGS 350
    GDIMLTCFVN LSRNRNVGLR LGSGEKLDEI MNSMNQVAEG VSTAGAVIAL 400
    AQKYHVKMPV LTAVARIIDN ELTPKKAVME LMNLPQVEEV 440
    Length:440
    Mass (Da):46,762
    Last modified:November 28, 2012 - v3
    Checksum:i5A1D054CC6312A30
    GO

    Sequence cautioni

    The sequence BAG89576.1 differs from that shown. Reason: Sequencing errors.
    The sequence BAC84439.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence BAF21142.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP005764 Genomic DNA. Translation: BAC84439.1. Sequence problems.
    AP008213 Genomic DNA. Translation: BAF21142.1. Sequence problems.
    AK065591 mRNA. Translation: BAG89576.1. Sequence problems.
    RefSeqiNP_001059228.1. NM_001065763.1.
    UniGeneiOs.18760.

    Genome annotation databases

    GeneIDi4342770.
    KEGGiosa:4342770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP005764 Genomic DNA. Translation: BAC84439.1 . Sequence problems.
    AP008213 Genomic DNA. Translation: BAF21142.1 . Sequence problems.
    AK065591 mRNA. Translation: BAG89576.1 . Sequence problems.
    RefSeqi NP_001059228.1. NM_001065763.1.
    UniGenei Os.18760.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os07g12640.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4342770.
    KEGGi osa:4342770.

    Organism-specific databases

    Gramenei Q8H2J9.

    Phylogenomic databases

    HOGENOMi HOG000246853.

    Enzyme and pathway databases

    UniPathwayi UPA00940 .

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_00394. NAD_Glyc3P_dehydrog.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    PROSITEi PS00957. NAD_G3PDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiGPDA_ORYSJ
    AccessioniPrimary (citable) accession number: Q8H2J9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: November 28, 2012
    Last modified: October 1, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3