Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial

Gene

IAR4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Enzyme regulationi

E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.By similarity

GO - Molecular functioni

  1. cobalt ion binding Source: TAIR
  2. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC
  3. zinc ion binding Source: TAIR

GO - Biological processi

  1. glycolytic process Source: UniProtKB-KW
  2. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Pyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciARA:AT1G24180-MONOMER.
ReactomeiREACT_235684. Regulation of pyruvate dehydrogenase (PDH) complex.
REACT_242640. Pyruvate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial (EC:1.2.4.1)
Short name:
PDHE1-A
Alternative name(s):
Protein IAA-CONJUGATE-RESISTANT 4
Gene namesi
Name:IAR4
Ordered Locus Names:At1g24180
ORF Names:F3I6.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G24180.

Subcellular locationi

Mitochondrion matrix 1 Publication

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. mitochondrial matrix Source: UniProtKB-SubCell
  3. mitochondrion Source: TAIR
  4. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Reduced sensitivity to several IAA-amino acid conjugates.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi121 – 1211R → C in iar4-1; reduced sensitivity to several IAA-amino acid conjugates. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828MitochondrionSequence AnalysisAdd
BLAST
Chaini29 – 393365Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrialPRO_0000260024Add
BLAST

Proteomic databases

PaxDbiQ8H1Y0.
PRIDEiQ8H1Y0.

Expressioni

Gene expression databases

GenevestigatoriQ8H1Y0.

Interactioni

Subunit structurei

Tetramer of 2 alpha and 2 beta subunits.By similarity

Protein-protein interaction databases

MINTiMINT-8063590.

Structurei

3D structure databases

ProteinModelPortaliQ8H1Y0.
SMRiQ8H1Y0. Positions 48-385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1071.
HOGENOMiHOG000281336.
InParanoidiQ8H1Y0.
KOiK00161.
OMAiHLCESPS.
PhylomeDBiQ8H1Y0.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR017597. Pyrv_DH_E1_asu_subgrp-y.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
TIGRFAMsiTIGR03182. PDH_E1_alph_y. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8H1Y0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSRLSSRS NTFLKPAITA LPSSIRRHVS TDSSPITIET AVPFTSHLCE
60 70 80 90 100
SPSRSVETSS EEILAFFRDM ARMRRMEIAA DSLYKAKLIR GFCHLYDGQE
110 120 130 140 150
ALAVGMEAAI TKKDAIITSY RDHCTFIGRG GKLVDAFSEL MGRKTGCSHG
160 170 180 190 200
KGGSMHFYKK DASFYGGHGI VGAQIPLGCG LAFAQKYNKD EAVTFALYGD
210 220 230 240 250
GAANQGQLFE ALNISALWDL PAILVCENNH YGMGTATWRS AKSPAYFKRG
260 270 280 290 300
DYVPGLKVDG MDALAVKQAC KFAKEHALKN GPIILEMDTY RYHGHSMSDP
310 320 330 340 350
GSTYRTRDEI SGVRQVRDPI ERVRKLLLTH DIATEKELKD MEKEIRKEVD
360 370 380 390
DAVAQAKESP IPDASELFTN MYVKDCGVES FGADRKELKV TLP
Length:393
Mass (Da):43,359
Last modified:November 28, 2006 - v2
Checksum:i1B28971865B52817
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti231 – 2311Y → D in AAN15218 (PubMed:15173569).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135561 mRNA. Translation: AAN15218.1.
AC002396 Genomic DNA. Translation: AAC00577.1.
CP002684 Genomic DNA. Translation: AEE30492.1.
AF360215 mRNA. Translation: AAK25925.1.
AY051018 mRNA. Translation: AAK93695.1.
AY088101 mRNA. Translation: AAM65647.1.
PIRiT00648.
RefSeqiNP_173828.1. NM_102264.4.
UniGeneiAt.24830.

Genome annotation databases

EnsemblPlantsiAT1G24180.1; AT1G24180.1; AT1G24180.
GeneIDi839031.
KEGGiath:AT1G24180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135561 mRNA. Translation: AAN15218.1.
AC002396 Genomic DNA. Translation: AAC00577.1.
CP002684 Genomic DNA. Translation: AEE30492.1.
AF360215 mRNA. Translation: AAK25925.1.
AY051018 mRNA. Translation: AAK93695.1.
AY088101 mRNA. Translation: AAM65647.1.
PIRiT00648.
RefSeqiNP_173828.1. NM_102264.4.
UniGeneiAt.24830.

3D structure databases

ProteinModelPortaliQ8H1Y0.
SMRiQ8H1Y0. Positions 48-385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8063590.

Proteomic databases

PaxDbiQ8H1Y0.
PRIDEiQ8H1Y0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G24180.1; AT1G24180.1; AT1G24180.
GeneIDi839031.
KEGGiath:AT1G24180.

Organism-specific databases

GeneFarmi4373. 441.
TAIRiAT1G24180.

Phylogenomic databases

eggNOGiCOG1071.
HOGENOMiHOG000281336.
InParanoidiQ8H1Y0.
KOiK00161.
OMAiHLCESPS.
PhylomeDBiQ8H1Y0.

Enzyme and pathway databases

BioCyciARA:AT1G24180-MONOMER.
ReactomeiREACT_235684. Regulation of pyruvate dehydrogenase (PDH) complex.
REACT_242640. Pyruvate metabolism.

Gene expression databases

GenevestigatoriQ8H1Y0.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR017597. Pyrv_DH_E1_asu_subgrp-y.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
TIGRFAMsiTIGR03182. PDH_E1_alph_y. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "IAR4, a gene required for auxin conjugate sensitivity in Arabidopsis, encodes a pyruvate dehydrogenase E1alpha homolog."
    LeClere S., Rampey R.A., Bartel B.
    Plant Physiol. 135:989-999(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ARG-121, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiODPA2_ARATH
AccessioniPrimary (citable) accession number: Q8H1Y0
Secondary accession number(s): O48685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: January 7, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.