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Q8H1Y0 (ODPA2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial

Short name=PDHE1-A
EC=1.2.4.1
Alternative name(s):
Protein IAA-CONJUGATE-RESISTANT 4
Gene names
Name:IAR4
Ordered Locus Names:At1g24180
ORF Names:F3I6.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activity

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactor

Thiamine pyrophosphate.

Enzyme regulation

E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit By similarity.

Subunit structure

Tetramer of 2 alpha and 2 beta subunits By similarity.

Subcellular location

Mitochondrion matrix Ref.6.

Disruption phenotype

Reduced sensitivity to several IAA-amino acid conjugates. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2828Mitochondrion Potential
Chain29 – 393365Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial
PRO_0000260024

Experimental info

Mutagenesis1211R → C in iar4-1; reduced sensitivity to several IAA-amino acid conjugates. Ref.1
Sequence conflict2311Y → D in AAN15218. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8H1Y0 [UniParc].

Last modified November 28, 2006. Version 2.
Checksum: 1B28971865B52817

FASTA39343,359
        10         20         30         40         50         60 
MALSRLSSRS NTFLKPAITA LPSSIRRHVS TDSSPITIET AVPFTSHLCE SPSRSVETSS 

        70         80         90        100        110        120 
EEILAFFRDM ARMRRMEIAA DSLYKAKLIR GFCHLYDGQE ALAVGMEAAI TKKDAIITSY 

       130        140        150        160        170        180 
RDHCTFIGRG GKLVDAFSEL MGRKTGCSHG KGGSMHFYKK DASFYGGHGI VGAQIPLGCG 

       190        200        210        220        230        240 
LAFAQKYNKD EAVTFALYGD GAANQGQLFE ALNISALWDL PAILVCENNH YGMGTATWRS 

       250        260        270        280        290        300 
AKSPAYFKRG DYVPGLKVDG MDALAVKQAC KFAKEHALKN GPIILEMDTY RYHGHSMSDP 

       310        320        330        340        350        360 
GSTYRTRDEI SGVRQVRDPI ERVRKLLLTH DIATEKELKD MEKEIRKEVD DAVAQAKESP 

       370        380        390 
IPDASELFTN MYVKDCGVES FGADRKELKV TLP 

« Hide

References

« Hide 'large scale' references
[1]"IAR4, a gene required for auxin conjugate sensitivity in Arabidopsis, encodes a pyruvate dehydrogenase E1alpha homolog."
LeClere S., Rampey R.A., Bartel B.
Plant Physiol. 135:989-999(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ARG-121, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY135561 mRNA. Translation: AAN15218.1.
AC002396 Genomic DNA. Translation: AAC00577.1.
CP002684 Genomic DNA. Translation: AEE30492.1.
AF360215 mRNA. Translation: AAK25925.1.
AY051018 mRNA. Translation: AAK93695.1.
AY088101 mRNA. Translation: AAM65647.1.
PIRT00648.
RefSeqNP_173828.1. NM_102264.4.
UniGeneAt.24830.

3D structure databases

ProteinModelPortalQ8H1Y0.
SMRQ8H1Y0. Positions 48-385.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8063590.

Proteomic databases

PaxDbQ8H1Y0.
PRIDEQ8H1Y0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G24180.1; AT1G24180.1; AT1G24180.
GeneID839031.
KEGGath:AT1G24180.

Organism-specific databases

GeneFarm4373. 441.
TAIRAT1G24180.

Phylogenomic databases

eggNOGCOG1071.
HOGENOMHOG000281336.
InParanoidQ8H1Y0.
KOK00161.
OMAHLCESPS.
PhylomeDBQ8H1Y0.

Enzyme and pathway databases

BioCycARA:AT1G24180-MONOMER.

Gene expression databases

GenevestigatorQ8H1Y0.

Family and domain databases

Gene3D3.40.50.970. 1 hit.
InterProIPR001017. DH_E1.
IPR017597. Pyrv_DH_E1_asu_subgrp-y.
IPR029061. THDP-binding.
[Graphical view]
PfamPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMSSF52518. SSF52518. 1 hit.
TIGRFAMsTIGR03182. PDH_E1_alph_y. 1 hit.
ProtoNetSearch...

Entry information

Entry nameODPA2_ARATH
AccessionPrimary (citable) accession number: Q8H1Y0
Secondary accession number(s): O48685
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: June 11, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names