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Q8H1Y0

- ODPA2_ARATH

UniProt

Q8H1Y0 - ODPA2_ARATH

Protein

Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial

Gene

IAR4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

    Catalytic activityi

    Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

    Cofactori

    Thiamine pyrophosphate.

    Enzyme regulationi

    E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.By similarity

    GO - Molecular functioni

    1. cobalt ion binding Source: TAIR
    2. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC
    3. zinc ion binding Source: TAIR

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-KW
    2. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Pyruvate, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciARA:AT1G24180-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial (EC:1.2.4.1)
    Short name:
    PDHE1-A
    Alternative name(s):
    Protein IAA-CONJUGATE-RESISTANT 4
    Gene namesi
    Name:IAR4
    Ordered Locus Names:At1g24180
    ORF Names:F3I6.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G24180.

    Subcellular locationi

    Mitochondrion matrix 1 Publication

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. mitochondrial matrix Source: UniProtKB-SubCell
    3. mitochondrion Source: TAIR
    4. nucleus Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Reduced sensitivity to several IAA-amino acid conjugates.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi121 – 1211R → C in iar4-1; reduced sensitivity to several IAA-amino acid conjugates. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2828MitochondrionSequence AnalysisAdd
    BLAST
    Chaini29 – 393365Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrialPRO_0000260024Add
    BLAST

    Proteomic databases

    PaxDbiQ8H1Y0.
    PRIDEiQ8H1Y0.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8H1Y0.

    Interactioni

    Subunit structurei

    Tetramer of 2 alpha and 2 beta subunits.By similarity

    Protein-protein interaction databases

    MINTiMINT-8063590.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H1Y0.
    SMRiQ8H1Y0. Positions 48-385.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1071.
    HOGENOMiHOG000281336.
    InParanoidiQ8H1Y0.
    KOiK00161.
    OMAiHLCESPS.
    PhylomeDBiQ8H1Y0.

    Family and domain databases

    Gene3Di3.40.50.970. 1 hit.
    InterProiIPR001017. DH_E1.
    IPR017597. Pyrv_DH_E1_asu_subgrp-y.
    IPR029061. THDP-binding.
    [Graphical view]
    PfamiPF00676. E1_dh. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 1 hit.
    TIGRFAMsiTIGR03182. PDH_E1_alph_y. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8H1Y0-1 [UniParc]FASTAAdd to Basket

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    MALSRLSSRS NTFLKPAITA LPSSIRRHVS TDSSPITIET AVPFTSHLCE    50
    SPSRSVETSS EEILAFFRDM ARMRRMEIAA DSLYKAKLIR GFCHLYDGQE 100
    ALAVGMEAAI TKKDAIITSY RDHCTFIGRG GKLVDAFSEL MGRKTGCSHG 150
    KGGSMHFYKK DASFYGGHGI VGAQIPLGCG LAFAQKYNKD EAVTFALYGD 200
    GAANQGQLFE ALNISALWDL PAILVCENNH YGMGTATWRS AKSPAYFKRG 250
    DYVPGLKVDG MDALAVKQAC KFAKEHALKN GPIILEMDTY RYHGHSMSDP 300
    GSTYRTRDEI SGVRQVRDPI ERVRKLLLTH DIATEKELKD MEKEIRKEVD 350
    DAVAQAKESP IPDASELFTN MYVKDCGVES FGADRKELKV TLP 393
    Length:393
    Mass (Da):43,359
    Last modified:November 28, 2006 - v2
    Checksum:i1B28971865B52817
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti231 – 2311Y → D in AAN15218. (PubMed:15173569)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY135561 mRNA. Translation: AAN15218.1.
    AC002396 Genomic DNA. Translation: AAC00577.1.
    CP002684 Genomic DNA. Translation: AEE30492.1.
    AF360215 mRNA. Translation: AAK25925.1.
    AY051018 mRNA. Translation: AAK93695.1.
    AY088101 mRNA. Translation: AAM65647.1.
    PIRiT00648.
    RefSeqiNP_173828.1. NM_102264.4.
    UniGeneiAt.24830.

    Genome annotation databases

    EnsemblPlantsiAT1G24180.1; AT1G24180.1; AT1G24180.
    GeneIDi839031.
    KEGGiath:AT1G24180.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY135561 mRNA. Translation: AAN15218.1 .
    AC002396 Genomic DNA. Translation: AAC00577.1 .
    CP002684 Genomic DNA. Translation: AEE30492.1 .
    AF360215 mRNA. Translation: AAK25925.1 .
    AY051018 mRNA. Translation: AAK93695.1 .
    AY088101 mRNA. Translation: AAM65647.1 .
    PIRi T00648.
    RefSeqi NP_173828.1. NM_102264.4.
    UniGenei At.24830.

    3D structure databases

    ProteinModelPortali Q8H1Y0.
    SMRi Q8H1Y0. Positions 48-385.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8063590.

    Proteomic databases

    PaxDbi Q8H1Y0.
    PRIDEi Q8H1Y0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G24180.1 ; AT1G24180.1 ; AT1G24180 .
    GeneIDi 839031.
    KEGGi ath:AT1G24180.

    Organism-specific databases

    GeneFarmi 4373. 441.
    TAIRi AT1G24180.

    Phylogenomic databases

    eggNOGi COG1071.
    HOGENOMi HOG000281336.
    InParanoidi Q8H1Y0.
    KOi K00161.
    OMAi HLCESPS.
    PhylomeDBi Q8H1Y0.

    Enzyme and pathway databases

    BioCyci ARA:AT1G24180-MONOMER.

    Gene expression databases

    Genevestigatori Q8H1Y0.

    Family and domain databases

    Gene3Di 3.40.50.970. 1 hit.
    InterProi IPR001017. DH_E1.
    IPR017597. Pyrv_DH_E1_asu_subgrp-y.
    IPR029061. THDP-binding.
    [Graphical view ]
    Pfami PF00676. E1_dh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 1 hit.
    TIGRFAMsi TIGR03182. PDH_E1_alph_y. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "IAR4, a gene required for auxin conjugate sensitivity in Arabidopsis, encodes a pyruvate dehydrogenase E1alpha homolog."
      LeClere S., Rampey R.A., Bartel B.
      Plant Physiol. 135:989-999(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ARG-121, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiODPA2_ARATH
    AccessioniPrimary (citable) accession number: Q8H1Y0
    Secondary accession number(s): O48685
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names

    External Data

    Dasty 3