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Q8H1U5 (APC2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anaphase-promoting complex subunit 2
Alternative name(s):
Cyclosome subunit 2
Gene names
Name:APC2
Ordered Locus Names:At2g04660
ORF Names:F28I8.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length865 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex controls several key steps in the cell cycle by mediating ubiquitination and subsequent degradation of target proteins such as cyclins. The APC/C complex is required for the female gametophyte development and is involved in several aspect of development by controlling cell division and cell elongation. Involved in the control of endoreduplication. Ref.5

Pathway

Protein modification; protein ubiquitination.

Subunit structure

The APC/C is composed of at least 10 subunits. Interacts with APC8, APC11, CDC27A and CDC27B. Ref.5 Ref.6

Subcellular location

Nucleus Ref.5.

Tissue specificity

Highly expressed in immature flowers. Expressed in stems, leaves and flowers. Ref.5

Disruption phenotype

Gametophytic lethal phenotype. Ref.5

Sequence similarities

Belongs to the cullin family.

Sequence caution

The sequence AAD22340.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 865865Anaphase-promoting complex subunit 2
PRO_0000396838

Experimental info

Sequence conflict2471V → A in BAC43061. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8H1U5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 277D9DD60E4399C3

FASTA86597,791
        10         20         30         40         50         60 
MEALGSSDCN LEILETLSDD AIQEITESYD GFFTTVESLI AGTGDSLVED EFVSHVYCLC 

        70         80         90        100        110        120 
KYGLDSLVRD HFLRSLEQAF EKGGASSFWQ HFDAYSEKKH HNYGEEIQIV LCKALEEISI 

       130        140        150        160        170        180 
EKQYHEKCLS IVVHALQSFK EQSSDDRQNS DTERVHLFSR FQSMLSSTLM TTLPQHFPEI 

       190        200        210        220        230        240 
LHWYFKERLE ELSAIMDGDG IEEQEDDCMD LDEKLRYKNG EMDVDEGCSQ GKRLGHDKLV 

       250        260        270        280        290        300 
KNIGKVVRDL RSIGFTSMAE NAYASAIFLL LKAKVHDLAG DDYRTSVLES IKEWIQTVPL 

       310        320        330        340        350        360 
QFLNALLSYL GDSVSYGTTS SGLTSPLACC PSPSFSRVVT PSEGIVRWKL RLEYFAYETL 

       370        380        390        400        410        420 
QDLRIAKLFE IIVDYPESSP AIEDLKQCLE YTGQHSKLVE SFISSLKYRL LTAGASTNDI 

       430        440        450        460        470        480 
LHQYVSTIKA LRAIDPAGVF LEAVGEPIRD YLRGRKDTIK CIVTMLTDGS GGNANGSGNP 

       490        500        510        520        530        540 
GDSLLEELMR DEESQENVGF DDDFHTDDKQ AWINASRWEP DPVEADPLKG SLSQRKVDIL 

       550        560        570        580        590        600 
GMLVDIIGSK EQLVNEYRVM LAEKLLNKTD YDIDTEIRTV ELLKIHFGEA SMQRCEIMLN 

       610        620        630        640        650        660 
DLIDSKRVNT NIKKASQTGA ELRENELSVD TLTSTILSTN FWPPIQDEPL ELPGPVDKLL 

       670        680        690        700        710        720 
SDYANRYHEI KTPRKLLWKK NLGTVKLELQ FEDRAMQFTV SPTHAAIIMQ FQEKKSWTYK 

       730        740        750        760        770        780 
DLAEVIGIPI DALNRRVNFW ISKGVLREST GANSNSSVLT LVESITDSGK NEGEELLTGE 

       790        800        810        820        830        840 
EEGETSIASV EDQLRKEMTI YEKFIMGMLT NFGSMALERI HNTLKMFCVA DPSYDKSLQQ 

       850        860 
LQSFLSGLVS EEKLEFRDGM YLLKK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"The Arabidopsis anaphase-promoting complex or cyclosome: molecular and genetic characterization of the APC2 subunit."
Capron A., Serralbo O., Fulop K., Frugier F., Parmentier Y., Dong A., Lecureuil A., Guerche P., Kondorosi E., Scheres B., Genschik P.
Plant Cell 15:2370-2382(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH APC8 AND APC11, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]"Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C)."
Perez-Perez J.M., Serralbo O., Vanstraelen M., Gonzalez C., Criqui M.C., Genschik P., Kondorosi E., Scheres B.
Plant J. 53:78-89(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CDC27A AND CDC27B.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006955 Genomic DNA. Translation: AAD22340.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05853.1.
AK118453 mRNA. Translation: BAC43061.1.
AY138228 mRNA. Translation: AAN10196.1.
PIRH84459.
RefSeqNP_178543.2. NM_126495.2.
UniGeneAt.41283.

3D structure databases

ProteinModelPortalQ8H1U5.
SMRQ8H1U5. Positions 534-735.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid409. 18 interactions.
IntActQ8H1U5. 19 interactions.
STRING3702.AT2G04660.1-P.

Proteomic databases

PaxDbQ8H1U5.
PRIDEQ8H1U5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G04660.1; AT2G04660.1; AT2G04660.
GeneID815008.
KEGGath:AT2G04660.

Organism-specific databases

TAIRAT2G04660.

Phylogenomic databases

eggNOGCOG5647.
HOGENOMHOG000265303.
InParanoidQ8H1U5.
KOK03349.
OMAESSMQKC.
PhylomeDBQ8H1U5.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ8H1U5.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR014786. APC_su2_C.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF08672. APC2. 1 hit.
PF00888. Cullin. 1 hit.
[Graphical view]
SMARTSM01013. APC2. 1 hit.
[Graphical view]
SUPFAMSSF75632. SSF75632. 1 hit.
PROSITEPS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPC2_ARATH
AccessionPrimary (citable) accession number: Q8H1U5
Secondary accession number(s): Q8GX39, Q9SJ93
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names