Q8H1U5 (APC2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anaphase-promoting complex subunit 2

Alternative name(s):
Cyclosome subunit 2

Gene names

Name:	APC2
Ordered Locus Names:	At2g04660
ORF Names:	F28I8.30

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	865 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex controls several key steps in the cell cycle by mediating ubiquitination and subsequent degradation of target proteins such as cyclins. The APC/C complex is required for the female gametophyte development and is involved in several aspect of development by controlling cell division and cell elongation. Involved in the control of endoreduplication. Ref.5
Pathway	Protein modification; protein ubiquitination.
Subunit structure	The APC/C is composed of at least 10 subunits. Interacts with APC8, APC11, CDC27A and CDC27B. Ref.5 Ref.6
Subcellular location	Nucleus Ref.5.
Tissue specificity	Highly expressed in immature flowers. Expressed in stems, leaves and flowers. Ref.5
Disruption phenotype	Gametophytic lethal phenotype. Ref.5
Sequence similarities	Belongs to the cullin family.
Sequence caution	The sequence AAD22340.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW megagametogenesis Inferred from mutant phenotype Ref.5. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cullin-RING ubiquitin ligase complex Inferred from electronic annotation. Source: InterPro nucleus Inferred from direct assay Ref.5. Source: UniProtKB spindle Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APC11	Q9M9L0	4	EBI-1749410,EBI-2130744
APC8	Q9STS3	3	EBI-1749410,EBI-2130714
CDC27A	Q06AN9	2	EBI-1749410,EBI-1749373
CDC27B	Q8LGU6	3	EBI-1749410,EBI-1668733

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 865	865	Anaphase-promoting complex subunit 2		PRO_0000396838
Experimental info
	Sequence conflict	247	1	V → A in BAC43061. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8H1U5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 277D9DD60E4399C3	FASTA	865	97,791
	10 20 30 40 50 60 MEALGSSDCN LEILETLSDD AIQEITESYD GFFTTVESLI AGTGDSLVED EFVSHVYCLC 70 80 90 100 110 120 KYGLDSLVRD HFLRSLEQAF EKGGASSFWQ HFDAYSEKKH HNYGEEIQIV LCKALEEISI 130 140 150 160 170 180 EKQYHEKCLS IVVHALQSFK EQSSDDRQNS DTERVHLFSR FQSMLSSTLM TTLPQHFPEI 190 200 210 220 230 240 LHWYFKERLE ELSAIMDGDG IEEQEDDCMD LDEKLRYKNG EMDVDEGCSQ GKRLGHDKLV 250 260 270 280 290 300 KNIGKVVRDL RSIGFTSMAE NAYASAIFLL LKAKVHDLAG DDYRTSVLES IKEWIQTVPL 310 320 330 340 350 360 QFLNALLSYL GDSVSYGTTS SGLTSPLACC PSPSFSRVVT PSEGIVRWKL RLEYFAYETL 370 380 390 400 410 420 QDLRIAKLFE IIVDYPESSP AIEDLKQCLE YTGQHSKLVE SFISSLKYRL LTAGASTNDI 430 440 450 460 470 480 LHQYVSTIKA LRAIDPAGVF LEAVGEPIRD YLRGRKDTIK CIVTMLTDGS GGNANGSGNP 490 500 510 520 530 540 GDSLLEELMR DEESQENVGF DDDFHTDDKQ AWINASRWEP DPVEADPLKG SLSQRKVDIL 550 560 570 580 590 600 GMLVDIIGSK EQLVNEYRVM LAEKLLNKTD YDIDTEIRTV ELLKIHFGEA SMQRCEIMLN 610 620 630 640 650 660 DLIDSKRVNT NIKKASQTGA ELRENELSVD TLTSTILSTN FWPPIQDEPL ELPGPVDKLL 670 680 690 700 710 720 SDYANRYHEI KTPRKLLWKK NLGTVKLELQ FEDRAMQFTV SPTHAAIIMQ FQEKKSWTYK 730 740 750 760 770 780 DLAEVIGIPI DALNRRVNFW ISKGVLREST GANSNSSVLT LVESITDSGK NEGEELLTGE 790 800 810 820 830 840 EEGETSIASV EDQLRKEMTI YEKFIMGMLT NFGSMALERI HNTLKMFCVA DPSYDKSLQQ 850 860 LQSFLSGLVS EEKLEFRDGM YLLKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The Arabidopsis anaphase-promoting complex or cyclosome: molecular and genetic characterization of the APC2 subunit." Capron A., Serralbo O., Fulop K., Frugier F., Parmentier Y., Dong A., Lecureuil A., Guerche P., Kondorosi E., Scheres B., Genschik P. Plant Cell 15:2370-2382(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH APC8 AND APC11, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]	"Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C)." Perez-Perez J.M., Serralbo O., Vanstraelen M., Gonzalez C., Criqui M.C., Genschik P., Kondorosi E., Scheres B. Plant J. 53:78-89(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDC27A AND CDC27B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006955 Genomic DNA. Translation: AAD22340.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC05853.1. AK118453 mRNA. Translation: BAC43061.1. AY138228 mRNA. Translation: AAN10196.1.
IPI	IPI00520644.
PIR	H84459.
RefSeq	NP_178543.2. NM_126495.2.
UniGene	At.41283.
3D structure databases
ProteinModelPortal	Q8H1U5.
SMR	Q8H1U5. Positions 534-735.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8H1U5. 19 interactions.
STRING	3702.AT2G04660.1-P.
Proteomic databases
PaxDb	Q8H1U5.
PRIDE	Q8H1U5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G04660.1; AT2G04660.1; AT2G04660.
GeneID	815008.
KEGG	ath:AT2G04660.
Organism-specific databases
TAIR	At2g04660.
Phylogenomic databases
eggNOG	COG5647.
HOGENOM	HOG000265303.
InParanoid	Q8H1U5.
KO	K03349.
OMA	RIHNTLK.
PhylomeDB	Q8H1U5.
ProtClustDB	CLSN2690638.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q8H1U5.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR014786. APC_su2_C. IPR016158. Cullin_homology. IPR001373. Cullin_N. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF08672. APC2. 1 hit. PF00888. Cullin. 1 hit. [Graphical view]
SMART	SM01013. APC2. 1 hit. [Graphical view]
SUPFAM	SSF75632. Cullin_homology. 1 hit.
PROSITE	PS01256. CULLIN_1. False negative. PS50069. CULLIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APC2_ARATH

Accession

Primary (citable) accession number: Q8H1U5
Secondary accession number(s): Q8GX39, Q9SJ93

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program