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Protein

Anaphase-promoting complex subunit 2

Gene

APC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex controls several key steps in the cell cycle by mediating ubiquitination and subsequent degradation of target proteins such as cyclins. The APC/C complex is required for the female gametophyte development and is involved in several aspect of development by controlling cell division and cell elongation. Involved in the control of endoreduplication.1 Publication

Pathwayi

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. megagametogenesis Source: UniProtKB
  3. mitotic nuclear division Source: UniProtKB-KW
  4. protein ubiquitination Source: UniProtKB-UniPathway
  5. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_241531. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_246699. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_249531. Regulation of APC/C activators between G1/S and early anaphase.
REACT_250339. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 2
Alternative name(s):
Cyclosome subunit 2
Gene namesi
Name:APC2
Ordered Locus Names:At2g04660
ORF Names:F28I8.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G04660.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cullin-RING ubiquitin ligase complex Source: InterPro
  2. nucleus Source: UniProtKB
  3. spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Gametophytic lethal phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 865865Anaphase-promoting complex subunit 2PRO_0000396838Add
BLAST

Proteomic databases

PaxDbiQ8H1U5.
PRIDEiQ8H1U5.

Expressioni

Tissue specificityi

Highly expressed in immature flowers. Expressed in stems, leaves and flowers.1 Publication

Gene expression databases

GenevestigatoriQ8H1U5.

Interactioni

Subunit structurei

The APC/C is composed of at least 10 subunits. Interacts with APC8, APC11, CDC27A and CDC27B.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APC11Q9M9L04EBI-1749410,EBI-2130744
APC8Q9STS33EBI-1749410,EBI-2130714
CDC27AQ06AN92EBI-1749410,EBI-1749373
CDC27BQ8LGU63EBI-1749410,EBI-1668733

Protein-protein interaction databases

BioGridi409. 18 interactions.
IntActiQ8H1U5. 19 interactions.
STRINGi3702.AT2G04660.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8H1U5.
SMRiQ8H1U5. Positions 534-735.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5647.
HOGENOMiHOG000265303.
InParanoidiQ8H1U5.
KOiK03349.
OMAiYEKFIMG.
PhylomeDBiQ8H1U5.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR014786. APC_su2_C.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08672. APC2. 1 hit.
PF00888. Cullin. 1 hit.
[Graphical view]
SMARTiSM01013. APC2. 1 hit.
[Graphical view]
SUPFAMiSSF75632. SSF75632. 1 hit.
PROSITEiPS50069. CULLIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8H1U5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEALGSSDCN LEILETLSDD AIQEITESYD GFFTTVESLI AGTGDSLVED
60 70 80 90 100
EFVSHVYCLC KYGLDSLVRD HFLRSLEQAF EKGGASSFWQ HFDAYSEKKH
110 120 130 140 150
HNYGEEIQIV LCKALEEISI EKQYHEKCLS IVVHALQSFK EQSSDDRQNS
160 170 180 190 200
DTERVHLFSR FQSMLSSTLM TTLPQHFPEI LHWYFKERLE ELSAIMDGDG
210 220 230 240 250
IEEQEDDCMD LDEKLRYKNG EMDVDEGCSQ GKRLGHDKLV KNIGKVVRDL
260 270 280 290 300
RSIGFTSMAE NAYASAIFLL LKAKVHDLAG DDYRTSVLES IKEWIQTVPL
310 320 330 340 350
QFLNALLSYL GDSVSYGTTS SGLTSPLACC PSPSFSRVVT PSEGIVRWKL
360 370 380 390 400
RLEYFAYETL QDLRIAKLFE IIVDYPESSP AIEDLKQCLE YTGQHSKLVE
410 420 430 440 450
SFISSLKYRL LTAGASTNDI LHQYVSTIKA LRAIDPAGVF LEAVGEPIRD
460 470 480 490 500
YLRGRKDTIK CIVTMLTDGS GGNANGSGNP GDSLLEELMR DEESQENVGF
510 520 530 540 550
DDDFHTDDKQ AWINASRWEP DPVEADPLKG SLSQRKVDIL GMLVDIIGSK
560 570 580 590 600
EQLVNEYRVM LAEKLLNKTD YDIDTEIRTV ELLKIHFGEA SMQRCEIMLN
610 620 630 640 650
DLIDSKRVNT NIKKASQTGA ELRENELSVD TLTSTILSTN FWPPIQDEPL
660 670 680 690 700
ELPGPVDKLL SDYANRYHEI KTPRKLLWKK NLGTVKLELQ FEDRAMQFTV
710 720 730 740 750
SPTHAAIIMQ FQEKKSWTYK DLAEVIGIPI DALNRRVNFW ISKGVLREST
760 770 780 790 800
GANSNSSVLT LVESITDSGK NEGEELLTGE EEGETSIASV EDQLRKEMTI
810 820 830 840 850
YEKFIMGMLT NFGSMALERI HNTLKMFCVA DPSYDKSLQQ LQSFLSGLVS
860
EEKLEFRDGM YLLKK
Length:865
Mass (Da):97,791
Last modified:March 1, 2003 - v1
Checksum:i277D9DD60E4399C3
GO

Sequence cautioni

The sequence AAD22340.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti247 – 2471V → A in BAC43061. (PubMed:11910074)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006955 Genomic DNA. Translation: AAD22340.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05853.1.
AK118453 mRNA. Translation: BAC43061.1.
AY138228 mRNA. Translation: AAN10196.1.
PIRiH84459.
RefSeqiNP_178543.2. NM_126495.2.
UniGeneiAt.41283.

Genome annotation databases

EnsemblPlantsiAT2G04660.1; AT2G04660.1; AT2G04660.
GeneIDi815008.
KEGGiath:AT2G04660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006955 Genomic DNA. Translation: AAD22340.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05853.1.
AK118453 mRNA. Translation: BAC43061.1.
AY138228 mRNA. Translation: AAN10196.1.
PIRiH84459.
RefSeqiNP_178543.2. NM_126495.2.
UniGeneiAt.41283.

3D structure databases

ProteinModelPortaliQ8H1U5.
SMRiQ8H1U5. Positions 534-735.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi409. 18 interactions.
IntActiQ8H1U5. 19 interactions.
STRINGi3702.AT2G04660.1-P.

Proteomic databases

PaxDbiQ8H1U5.
PRIDEiQ8H1U5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G04660.1; AT2G04660.1; AT2G04660.
GeneIDi815008.
KEGGiath:AT2G04660.

Organism-specific databases

TAIRiAT2G04660.

Phylogenomic databases

eggNOGiCOG5647.
HOGENOMiHOG000265303.
InParanoidiQ8H1U5.
KOiK03349.
OMAiYEKFIMG.
PhylomeDBiQ8H1U5.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_241531. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_246699. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_249531. Regulation of APC/C activators between G1/S and early anaphase.
REACT_250339. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Gene expression databases

GenevestigatoriQ8H1U5.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR014786. APC_su2_C.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08672. APC2. 1 hit.
PF00888. Cullin. 1 hit.
[Graphical view]
SMARTiSM01013. APC2. 1 hit.
[Graphical view]
SUPFAMiSSF75632. SSF75632. 1 hit.
PROSITEiPS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The Arabidopsis anaphase-promoting complex or cyclosome: molecular and genetic characterization of the APC2 subunit."
    Capron A., Serralbo O., Fulop K., Frugier F., Parmentier Y., Dong A., Lecureuil A., Guerche P., Kondorosi E., Scheres B., Genschik P.
    Plant Cell 15:2370-2382(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH APC8 AND APC11, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  6. "Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C)."
    Perez-Perez J.M., Serralbo O., Vanstraelen M., Gonzalez C., Criqui M.C., Genschik P., Kondorosi E., Scheres B.
    Plant J. 53:78-89(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC27A AND CDC27B.

Entry informationi

Entry nameiAPC2_ARATH
AccessioniPrimary (citable) accession number: Q8H1U5
Secondary accession number(s): Q8GX39, Q9SJ93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: March 1, 2003
Last modified: February 4, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.