Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8H1U5

- APC2_ARATH

UniProt

Q8H1U5 - APC2_ARATH

Protein

Anaphase-promoting complex subunit 2

Gene

APC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex controls several key steps in the cell cycle by mediating ubiquitination and subsequent degradation of target proteins such as cyclins. The APC/C complex is required for the female gametophyte development and is involved in several aspect of development by controlling cell division and cell elongation. Involved in the control of endoreduplication.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. megagametogenesis Source: UniProtKB
    2. mitotic nuclear division Source: UniProtKB-KW
    3. protein ubiquitination Source: UniProtKB-UniPathway
    4. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anaphase-promoting complex subunit 2
    Alternative name(s):
    Cyclosome subunit 2
    Gene namesi
    Name:APC2
    Ordered Locus Names:At2g04660
    ORF Names:F28I8.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G04660.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cullin-RING ubiquitin ligase complex Source: InterPro
    2. nucleus Source: UniProtKB
    3. spindle Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Gametophytic lethal phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 865865Anaphase-promoting complex subunit 2PRO_0000396838Add
    BLAST

    Proteomic databases

    PaxDbiQ8H1U5.
    PRIDEiQ8H1U5.

    Expressioni

    Tissue specificityi

    Highly expressed in immature flowers. Expressed in stems, leaves and flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ8H1U5.

    Interactioni

    Subunit structurei

    The APC/C is composed of at least 10 subunits. Interacts with APC8, APC11, CDC27A and CDC27B.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APC11Q9M9L04EBI-1749410,EBI-2130744
    APC8Q9STS33EBI-1749410,EBI-2130714
    CDC27AQ06AN92EBI-1749410,EBI-1749373
    CDC27BQ8LGU63EBI-1749410,EBI-1668733

    Protein-protein interaction databases

    BioGridi409. 18 interactions.
    IntActiQ8H1U5. 19 interactions.
    STRINGi3702.AT2G04660.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H1U5.
    SMRiQ8H1U5. Positions 534-735.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cullin family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5647.
    HOGENOMiHOG000265303.
    InParanoidiQ8H1U5.
    KOiK03349.
    OMAiESSMQKC.
    PhylomeDBiQ8H1U5.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR014786. APC_su2_C.
    IPR016158. Cullin_homology.
    IPR001373. Cullin_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF08672. APC2. 1 hit.
    PF00888. Cullin. 1 hit.
    [Graphical view]
    SMARTiSM01013. APC2. 1 hit.
    [Graphical view]
    SUPFAMiSSF75632. SSF75632. 1 hit.
    PROSITEiPS50069. CULLIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8H1U5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEALGSSDCN LEILETLSDD AIQEITESYD GFFTTVESLI AGTGDSLVED    50
    EFVSHVYCLC KYGLDSLVRD HFLRSLEQAF EKGGASSFWQ HFDAYSEKKH 100
    HNYGEEIQIV LCKALEEISI EKQYHEKCLS IVVHALQSFK EQSSDDRQNS 150
    DTERVHLFSR FQSMLSSTLM TTLPQHFPEI LHWYFKERLE ELSAIMDGDG 200
    IEEQEDDCMD LDEKLRYKNG EMDVDEGCSQ GKRLGHDKLV KNIGKVVRDL 250
    RSIGFTSMAE NAYASAIFLL LKAKVHDLAG DDYRTSVLES IKEWIQTVPL 300
    QFLNALLSYL GDSVSYGTTS SGLTSPLACC PSPSFSRVVT PSEGIVRWKL 350
    RLEYFAYETL QDLRIAKLFE IIVDYPESSP AIEDLKQCLE YTGQHSKLVE 400
    SFISSLKYRL LTAGASTNDI LHQYVSTIKA LRAIDPAGVF LEAVGEPIRD 450
    YLRGRKDTIK CIVTMLTDGS GGNANGSGNP GDSLLEELMR DEESQENVGF 500
    DDDFHTDDKQ AWINASRWEP DPVEADPLKG SLSQRKVDIL GMLVDIIGSK 550
    EQLVNEYRVM LAEKLLNKTD YDIDTEIRTV ELLKIHFGEA SMQRCEIMLN 600
    DLIDSKRVNT NIKKASQTGA ELRENELSVD TLTSTILSTN FWPPIQDEPL 650
    ELPGPVDKLL SDYANRYHEI KTPRKLLWKK NLGTVKLELQ FEDRAMQFTV 700
    SPTHAAIIMQ FQEKKSWTYK DLAEVIGIPI DALNRRVNFW ISKGVLREST 750
    GANSNSSVLT LVESITDSGK NEGEELLTGE EEGETSIASV EDQLRKEMTI 800
    YEKFIMGMLT NFGSMALERI HNTLKMFCVA DPSYDKSLQQ LQSFLSGLVS 850
    EEKLEFRDGM YLLKK 865
    Length:865
    Mass (Da):97,791
    Last modified:March 1, 2003 - v1
    Checksum:i277D9DD60E4399C3
    GO

    Sequence cautioni

    The sequence AAD22340.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti247 – 2471V → A in BAC43061. (PubMed:11910074)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006955 Genomic DNA. Translation: AAD22340.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC05853.1.
    AK118453 mRNA. Translation: BAC43061.1.
    AY138228 mRNA. Translation: AAN10196.1.
    PIRiH84459.
    RefSeqiNP_178543.2. NM_126495.2.
    UniGeneiAt.41283.

    Genome annotation databases

    EnsemblPlantsiAT2G04660.1; AT2G04660.1; AT2G04660.
    GeneIDi815008.
    KEGGiath:AT2G04660.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006955 Genomic DNA. Translation: AAD22340.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC05853.1 .
    AK118453 mRNA. Translation: BAC43061.1 .
    AY138228 mRNA. Translation: AAN10196.1 .
    PIRi H84459.
    RefSeqi NP_178543.2. NM_126495.2.
    UniGenei At.41283.

    3D structure databases

    ProteinModelPortali Q8H1U5.
    SMRi Q8H1U5. Positions 534-735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 409. 18 interactions.
    IntActi Q8H1U5. 19 interactions.
    STRINGi 3702.AT2G04660.1-P.

    Proteomic databases

    PaxDbi Q8H1U5.
    PRIDEi Q8H1U5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G04660.1 ; AT2G04660.1 ; AT2G04660 .
    GeneIDi 815008.
    KEGGi ath:AT2G04660.

    Organism-specific databases

    TAIRi AT2G04660.

    Phylogenomic databases

    eggNOGi COG5647.
    HOGENOMi HOG000265303.
    InParanoidi Q8H1U5.
    KOi K03349.
    OMAi ESSMQKC.
    PhylomeDBi Q8H1U5.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Gene expression databases

    Genevestigatori Q8H1U5.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR014786. APC_su2_C.
    IPR016158. Cullin_homology.
    IPR001373. Cullin_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF08672. APC2. 1 hit.
    PF00888. Cullin. 1 hit.
    [Graphical view ]
    SMARTi SM01013. APC2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF75632. SSF75632. 1 hit.
    PROSITEi PS50069. CULLIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "The Arabidopsis anaphase-promoting complex or cyclosome: molecular and genetic characterization of the APC2 subunit."
      Capron A., Serralbo O., Fulop K., Frugier F., Parmentier Y., Dong A., Lecureuil A., Guerche P., Kondorosi E., Scheres B., Genschik P.
      Plant Cell 15:2370-2382(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH APC8 AND APC11, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    6. "Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C)."
      Perez-Perez J.M., Serralbo O., Vanstraelen M., Gonzalez C., Criqui M.C., Genschik P., Kondorosi E., Scheres B.
      Plant J. 53:78-89(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDC27A AND CDC27B.

    Entry informationi

    Entry nameiAPC2_ARATH
    AccessioniPrimary (citable) accession number: Q8H1U5
    Secondary accession number(s): Q8GX39, Q9SJ93
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3