Q8H1T3 (FRI2_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferritin-2, chloroplastic
EC=1.16.3.1

Alternative name(s):
NtFer2

Gene names

Name:

FER2

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation By similarity.
Catalytic activity	4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.
Subunit structure	Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the ferritin family. Contains 1 ferritin-like diiron domain.

Ontologies

Keywords
Biological process	Iron storage
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ferric iron binding Inferred from electronic annotation. Source: InterPro ferroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 52	52	Chloroplast Potential
	Chain	53 – 259	207	Ferritin-2, chloroplastic		PRO_0000008869
Regions
	Domain	86 – 239	154	Ferritin-like diiron
	Region	53 – 85	33	Extension peptide (EP)
Sites
	Metal binding	103	1	Iron 1 By similarity
	Metal binding	138	1	Iron 1 By similarity
	Metal binding	138	1	Iron 2 By similarity
	Metal binding	141	1	Iron 1 By similarity
	Metal binding	221	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8H1T3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 59997A45142375C3	FASTA	259	29,220
	10 20 30 40 50 60 MLLKLAPAFT LLNSHGENLS PMLSTSSQGF VLKNFSTKSR NGLLVVCASK GSNTKPLTGV 70 80 90 100 110 120 VFEPFEEVKK ELMLVPTVPQ VSLARHKYSD QCEAAVNEQI NVEYNVSYVY HGMYAYFDRD 130 140 150 160 170 180 NVALKGLARF FKESSEEERG HAEKLMEYQN KRGGKVKLQS ILMPLSEFDH AEEGDALYAM 190 200 210 220 230 240 ELALSLAKLT NQKLLNLHAV ATRNNDVQLA DFVESKYLRE QVEAIKMISE YVAQLRRVGK 250 GHGVWHFDQM LLQEEEVVA

References

[1]	"Occurrence and expression analysis of two types of the tobacco ferritin genes." Jiang T., Yoshihara T., Masuda T., Goto F. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY141105 mRNA. Translation: AAN06322.1.
3D structure databases
ProteinModelPortal	Q8H1T3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.1260.10. 1 hit.
InterPro	IPR001519. Ferritin. IPR009040. Ferritin-like_diiron. IPR009078. Ferritin-like_SF. IPR012347. Ferritin-rel. IPR014034. Ferritin_CS. IPR008331. Ferritin_DPS_dom. [Graphical view]
PANTHER	PTHR11431. PTHR11431. 1 hit.
Pfam	PF00210. Ferritin. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00540. FERRITIN_1. False negative. PS00204. FERRITIN_2. 1 hit. PS50905. FERRITIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRI2_TOBAC

Accession

Primary (citable) accession number: Q8H1T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program