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Protein

Inositol oxygenase 4

Gene

MIOX4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.2 Publications

Catalytic activityi

Myo-inositol + O2 = D-glucuronate + H2O.

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi: myo-inositol degradation into D-glucuronate

This protein is involved in step 1 of the subpathway that synthesizes D-glucuronate from myo-inositol.
Proteins known to be involved in this subpathway in this organism are:
  1. Inositol oxygenase 4 (MIOX4), Inositol oxygenase 2 (MIOX2), Inositol oxygenase 1 (MIOX1), Inositol oxygenase 5 (MIOX5)
This subpathway is part of the pathway myo-inositol degradation into D-glucuronate, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucuronate from myo-inositol, the pathway myo-inositol degradation into D-glucuronate and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58SubstrateBy similarity1
Metal bindingi128Iron 1By similarity1
Metal bindingi153Iron 1By similarity1
Metal bindingi154Iron 1By similarity1
Metal bindingi154Iron 2By similarity1
Binding sitei157SubstrateBy similarity1
Metal bindingi226Iron 2By similarity1
Metal bindingi252Iron 2By similarity1
Metal bindingi285Iron 1By similarity1

GO - Molecular functioni

  • inositol oxygenase activity Source: TAIR
  • iron ion binding Source: InterPro

GO - Biological processi

  • inositol catabolic process Source: InterPro
  • L-ascorbic acid biosynthetic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processAscorbate biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.99.1. 399.
ReactomeiR-ATH-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
UniPathwayiUPA00111; UER00527.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol oxygenase 4 (EC:1.13.99.1)
Alternative name(s):
Myo-inositol oxygenase 4
Short name:
AtMIOX4
Short name:
MI oxygenase 4
Gene namesi
Name:MIOX4
Ordered Locus Names:At4g26260
ORF Names:T25K17.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G26260.
TAIRilocus:2136839. AT4G26260.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000791561 – 317Inositol oxygenase 4Add BLAST317

Proteomic databases

PaxDbiQ8H1S0.

Expressioni

Tissue specificityi

Expressed in flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ8H1S0. baseline and differential.
GenevisibleiQ8H1S0. AT.

Interactioni

Protein-protein interaction databases

BioGridi14019. 1 interactor.
STRINGi3702.AT4G26260.2.

Structurei

3D structure databases

ProteinModelPortaliQ8H1S0.
SMRiQ8H1S0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 117Substrate bindingBy similarity3
Regioni174 – 175Substrate bindingBy similarity2
Regioni252 – 253Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the myo-inositol oxygenase family.Curated

Phylogenomic databases

eggNOGiKOG1573. Eukaryota.
ENOG410XQ4J. LUCA.
HOGENOMiHOG000163182.
InParanoidiQ8H1S0.
KOiK00469.
OMAiMTIQLEG.
PhylomeDBiQ8H1S0.

Family and domain databases

InterProiView protein in InterPro
IPR007828. Inositol_oxygenase.
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiView protein in Pfam
PF05153. MIOX. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8H1S0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTISVEKPIF EEVSAFEKSG DNIGELKLDG GFSMPKMDTN DDEAFLAPEM
60 70 80 90 100
NAFGRQFRDY DVESERQKGV EEFYRLQHIN QTVDFVKKMR AEYGKLDKMV
110 120 130 140 150
MSIWECCELL NEVVDESDPD LDEPQIQHLL QSAEAIRKDY PNEDWLHLTA
160 170 180 190 200
LIHDLGKVIT LPQFGGLPQW AVVGDTFPVG CAFDESNVHH KYFVENPDFH
210 220 230 240 250
NETYNTKNGI YSEGCGLNNV MMSWGHDDYM YLVAKENGST LPSAGQFIIR
260 270 280 290 300
YHSFYPLHTA GEYTHLMNEE DKENLKWLHV FNKYDLYSKS KVHVDVEKVK
310
PYYMSLIKKY FPENLRW
Length:317
Mass (Da):36,904
Last modified:March 1, 2003 - v1
Checksum:i59485B8A05BD2497
GO
Isoform 2 (identifier: Q8H1S0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: F → FE

Show »
Length:318
Mass (Da):37,033
Checksum:i7D7DF0A1B996DE67
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77Q → R in AAP59548 (PubMed:14976233).Curated1
Sequence conflicti300K → E in AAP59548 (PubMed:14976233).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04159110F → FE in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY232552 mRNA. Translation: AAP59548.1.
AL049171 Genomic DNA. Translation: CAB38955.1.
AL161564 Genomic DNA. Translation: CAB79481.1.
CP002687 Genomic DNA. Translation: AEE85176.1.
CP002687 Genomic DNA. Translation: AEE85177.1.
AY142501 mRNA. Translation: AAN13052.1.
PIRiT06010.
RefSeqiNP_001190844.1. NM_001203915.2. [Q8H1S0-2]
NP_194356.2. NM_118759.5. [Q8H1S0-1]
UniGeneiAt.32196.

Genome annotation databases

EnsemblPlantsiAT4G26260.1; AT4G26260.1; AT4G26260. [Q8H1S0-1]
AT4G26260.2; AT4G26260.2; AT4G26260. [Q8H1S0-2]
GeneIDi828732.
GrameneiAT4G26260.1; AT4G26260.1; AT4G26260.
AT4G26260.2; AT4G26260.2; AT4G26260.
KEGGiath:AT4G26260.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMIOX4_ARATH
AccessioniPrimary (citable) accession number: Q8H1S0
Secondary accession number(s): Q6XGZ9, Q9STQ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2003
Last modified: August 30, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families