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Q8H1S0

- MIOX4_ARATH

UniProt

Q8H1S0 - MIOX4_ARATH

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Protein
Inositol oxygenase 4
Gene
MIOX4, At4g26260, T25K17.70
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.2 Publications

Catalytic activityi

Myo-inositol + O2 = D-glucuronate + H2O.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei58 – 581Substrate By similarity
Metal bindingi128 – 1281Iron 1 By similarity
Metal bindingi153 – 1531Iron 1 By similarity
Metal bindingi154 – 1541Iron 1 By similarity
Metal bindingi154 – 1541Iron 2 By similarity
Binding sitei157 – 1571Substrate By similarity
Metal bindingi226 – 2261Iron 2 By similarity
Metal bindingi252 – 2521Iron 2 By similarity
Metal bindingi285 – 2851Iron 1 By similarity

GO - Molecular functioni

  1. inositol oxygenase activity Source: UniProtKB-EC
  2. iron ion binding Source: InterPro

GO - Biological processi

  1. L-ascorbic acid biosynthetic process Source: UniProtKB-KW
  2. inositol catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ascorbate biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:GQT-847-MONOMER.
BRENDAi1.13.99.1. 399.
UniPathwayiUPA00111; UER00527.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol oxygenase 4 (EC:1.13.99.1)
Alternative name(s):
Myo-inositol oxygenase 4
Short name:
AtMIOX4
Short name:
MI oxygenase 4
Gene namesi
Name:MIOX4
Ordered Locus Names:At4g26260
ORF Names:T25K17.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G26260.

Subcellular locationi

Cytoplasm Inferred

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Inositol oxygenase 4
PRO_0000079156Add
BLAST

Proteomic databases

PaxDbiQ8H1S0.
PRIDEiQ8H1S0.

Expressioni

Tissue specificityi

Expressed in flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ8H1S0.

Interactioni

Protein-protein interaction databases

BioGridi14019. 1 interaction.
STRINGi3702.AT4G26260.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8H1S0.
SMRiQ8H1S0. Positions 74-317.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni115 – 1173Substrate binding By similarity
Regioni174 – 1752Substrate binding By similarity
Regioni252 – 2532Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG135479.
HOGENOMiHOG000163182.
InParanoidiQ8H1S0.
KOiK00469.
OMAiFYRLQHI.
PhylomeDBiQ8H1S0.

Family and domain databases

InterProiIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. DUF706. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8H1S0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTISVEKPIF EEVSAFEKSG DNIGELKLDG GFSMPKMDTN DDEAFLAPEM    50
NAFGRQFRDY DVESERQKGV EEFYRLQHIN QTVDFVKKMR AEYGKLDKMV 100
MSIWECCELL NEVVDESDPD LDEPQIQHLL QSAEAIRKDY PNEDWLHLTA 150
LIHDLGKVIT LPQFGGLPQW AVVGDTFPVG CAFDESNVHH KYFVENPDFH 200
NETYNTKNGI YSEGCGLNNV MMSWGHDDYM YLVAKENGST LPSAGQFIIR 250
YHSFYPLHTA GEYTHLMNEE DKENLKWLHV FNKYDLYSKS KVHVDVEKVK 300
PYYMSLIKKY FPENLRW 317
Length:317
Mass (Da):36,904
Last modified:March 1, 2003 - v1
Checksum:i59485B8A05BD2497
GO
Isoform 2 (identifier: Q8H1S0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: F → FE

Show »
Length:318
Mass (Da):37,033
Checksum:i7D7DF0A1B996DE67
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei10 – 101F → FE in isoform 2.
VSP_041591

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771Q → R in AAP59548. 1 Publication
Sequence conflicti300 – 3001K → E in AAP59548. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY232552 mRNA. Translation: AAP59548.1.
AL049171 Genomic DNA. Translation: CAB38955.1.
AL161564 Genomic DNA. Translation: CAB79481.1.
CP002687 Genomic DNA. Translation: AEE85176.1.
CP002687 Genomic DNA. Translation: AEE85177.1.
AY142501 mRNA. Translation: AAN13052.1.
PIRiT06010.
RefSeqiNP_001190844.1. NM_001203915.1. [Q8H1S0-2]
NP_194356.2. NM_118759.4. [Q8H1S0-1]
UniGeneiAt.32196.

Genome annotation databases

EnsemblPlantsiAT4G26260.1; AT4G26260.1; AT4G26260. [Q8H1S0-1]
GeneIDi828732.
KEGGiath:AT4G26260.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY232552 mRNA. Translation: AAP59548.1 .
AL049171 Genomic DNA. Translation: CAB38955.1 .
AL161564 Genomic DNA. Translation: CAB79481.1 .
CP002687 Genomic DNA. Translation: AEE85176.1 .
CP002687 Genomic DNA. Translation: AEE85177.1 .
AY142501 mRNA. Translation: AAN13052.1 .
PIRi T06010.
RefSeqi NP_001190844.1. NM_001203915.1. [Q8H1S0-2 ]
NP_194356.2. NM_118759.4. [Q8H1S0-1 ]
UniGenei At.32196.

3D structure databases

ProteinModelPortali Q8H1S0.
SMRi Q8H1S0. Positions 74-317.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 14019. 1 interaction.
STRINGi 3702.AT4G26260.1-P.

Proteomic databases

PaxDbi Q8H1S0.
PRIDEi Q8H1S0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G26260.1 ; AT4G26260.1 ; AT4G26260 . [Q8H1S0-1 ]
GeneIDi 828732.
KEGGi ath:AT4G26260.

Organism-specific databases

TAIRi AT4G26260.

Phylogenomic databases

eggNOGi NOG135479.
HOGENOMi HOG000163182.
InParanoidi Q8H1S0.
KOi K00469.
OMAi FYRLQHI.
PhylomeDBi Q8H1S0.

Enzyme and pathway databases

UniPathwayi UPA00111 ; UER00527 .
BioCyci ARA:GQT-847-MONOMER.
BRENDAi 1.13.99.1. 399.

Gene expression databases

Genevestigatori Q8H1S0.

Family and domain databases

InterProi IPR007828. Inositol_oxygenase.
[Graphical view ]
PANTHERi PTHR12588. PTHR12588. 1 hit.
Pfami PF05153. DUF706. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis."
    Lorence A., Chevone B.I., Mendes P., Nessler C.L.
    Plant Physiol. 134:1200-1205(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
  2. "The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides."
    Kanter U., Usadel B., Guerineau F., Li Y., Pauly M., Tenhaken R.
    Planta 221:243-254(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.

Entry informationi

Entry nameiMIOX4_ARATH
AccessioniPrimary (citable) accession number: Q8H1S0
Secondary accession number(s): Q6XGZ9, Q9STQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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