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Q8H1S0

- MIOX4_ARATH

UniProt

Q8H1S0 - MIOX4_ARATH

Protein

Inositol oxygenase 4

Gene

MIOX4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.2 Publications

    Catalytic activityi

    Myo-inositol + O2 = D-glucuronate + H2O.

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei58 – 581SubstrateBy similarity
    Metal bindingi128 – 1281Iron 1By similarity
    Metal bindingi153 – 1531Iron 1By similarity
    Metal bindingi154 – 1541Iron 1By similarity
    Metal bindingi154 – 1541Iron 2By similarity
    Binding sitei157 – 1571SubstrateBy similarity
    Metal bindingi226 – 2261Iron 2By similarity
    Metal bindingi252 – 2521Iron 2By similarity
    Metal bindingi285 – 2851Iron 1By similarity

    GO - Molecular functioni

    1. inositol oxygenase activity Source: UniProtKB-EC
    2. iron ion binding Source: InterPro

    GO - Biological processi

    1. inositol catabolic process Source: InterPro
    2. L-ascorbic acid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Ascorbate biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-847-MONOMER.
    BRENDAi1.13.99.1. 399.
    UniPathwayiUPA00111; UER00527.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol oxygenase 4 (EC:1.13.99.1)
    Alternative name(s):
    Myo-inositol oxygenase 4
    Short name:
    AtMIOX4
    Short name:
    MI oxygenase 4
    Gene namesi
    Name:MIOX4
    Ordered Locus Names:At4g26260
    ORF Names:T25K17.70
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G26260.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Inositol oxygenase 4PRO_0000079156Add
    BLAST

    Proteomic databases

    PaxDbiQ8H1S0.
    PRIDEiQ8H1S0.

    Expressioni

    Tissue specificityi

    Expressed in flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ8H1S0.

    Interactioni

    Protein-protein interaction databases

    BioGridi14019. 1 interaction.
    STRINGi3702.AT4G26260.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H1S0.
    SMRiQ8H1S0. Positions 74-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni115 – 1173Substrate bindingBy similarity
    Regioni174 – 1752Substrate bindingBy similarity
    Regioni252 – 2532Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the myo-inositol oxygenase family.Curated

    Phylogenomic databases

    eggNOGiNOG135479.
    HOGENOMiHOG000163182.
    InParanoidiQ8H1S0.
    KOiK00469.
    OMAiFYRLQHI.
    PhylomeDBiQ8H1S0.

    Family and domain databases

    InterProiIPR007828. Inositol_oxygenase.
    [Graphical view]
    PANTHERiPTHR12588. PTHR12588. 1 hit.
    PfamiPF05153. DUF706. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8H1S0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTISVEKPIF EEVSAFEKSG DNIGELKLDG GFSMPKMDTN DDEAFLAPEM    50
    NAFGRQFRDY DVESERQKGV EEFYRLQHIN QTVDFVKKMR AEYGKLDKMV 100
    MSIWECCELL NEVVDESDPD LDEPQIQHLL QSAEAIRKDY PNEDWLHLTA 150
    LIHDLGKVIT LPQFGGLPQW AVVGDTFPVG CAFDESNVHH KYFVENPDFH 200
    NETYNTKNGI YSEGCGLNNV MMSWGHDDYM YLVAKENGST LPSAGQFIIR 250
    YHSFYPLHTA GEYTHLMNEE DKENLKWLHV FNKYDLYSKS KVHVDVEKVK 300
    PYYMSLIKKY FPENLRW 317
    Length:317
    Mass (Da):36,904
    Last modified:March 1, 2003 - v1
    Checksum:i59485B8A05BD2497
    GO
    Isoform 2 (identifier: Q8H1S0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         10-10: F → FE

    Show »
    Length:318
    Mass (Da):37,033
    Checksum:i7D7DF0A1B996DE67
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 771Q → R in AAP59548. (PubMed:14976233)Curated
    Sequence conflicti300 – 3001K → E in AAP59548. (PubMed:14976233)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei10 – 101F → FE in isoform 2. CuratedVSP_041591

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY232552 mRNA. Translation: AAP59548.1.
    AL049171 Genomic DNA. Translation: CAB38955.1.
    AL161564 Genomic DNA. Translation: CAB79481.1.
    CP002687 Genomic DNA. Translation: AEE85176.1.
    CP002687 Genomic DNA. Translation: AEE85177.1.
    AY142501 mRNA. Translation: AAN13052.1.
    PIRiT06010.
    RefSeqiNP_001190844.1. NM_001203915.1. [Q8H1S0-2]
    NP_194356.2. NM_118759.4. [Q8H1S0-1]
    UniGeneiAt.32196.

    Genome annotation databases

    EnsemblPlantsiAT4G26260.1; AT4G26260.1; AT4G26260. [Q8H1S0-1]
    GeneIDi828732.
    KEGGiath:AT4G26260.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY232552 mRNA. Translation: AAP59548.1 .
    AL049171 Genomic DNA. Translation: CAB38955.1 .
    AL161564 Genomic DNA. Translation: CAB79481.1 .
    CP002687 Genomic DNA. Translation: AEE85176.1 .
    CP002687 Genomic DNA. Translation: AEE85177.1 .
    AY142501 mRNA. Translation: AAN13052.1 .
    PIRi T06010.
    RefSeqi NP_001190844.1. NM_001203915.1. [Q8H1S0-2 ]
    NP_194356.2. NM_118759.4. [Q8H1S0-1 ]
    UniGenei At.32196.

    3D structure databases

    ProteinModelPortali Q8H1S0.
    SMRi Q8H1S0. Positions 74-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 14019. 1 interaction.
    STRINGi 3702.AT4G26260.1-P.

    Proteomic databases

    PaxDbi Q8H1S0.
    PRIDEi Q8H1S0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G26260.1 ; AT4G26260.1 ; AT4G26260 . [Q8H1S0-1 ]
    GeneIDi 828732.
    KEGGi ath:AT4G26260.

    Organism-specific databases

    TAIRi AT4G26260.

    Phylogenomic databases

    eggNOGi NOG135479.
    HOGENOMi HOG000163182.
    InParanoidi Q8H1S0.
    KOi K00469.
    OMAi FYRLQHI.
    PhylomeDBi Q8H1S0.

    Enzyme and pathway databases

    UniPathwayi UPA00111 ; UER00527 .
    BioCyci ARA:GQT-847-MONOMER.
    BRENDAi 1.13.99.1. 399.

    Gene expression databases

    Genevestigatori Q8H1S0.

    Family and domain databases

    InterProi IPR007828. Inositol_oxygenase.
    [Graphical view ]
    PANTHERi PTHR12588. PTHR12588. 1 hit.
    Pfami PF05153. DUF706. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis."
      Lorence A., Chevone B.I., Mendes P., Nessler C.L.
      Plant Physiol. 134:1200-1205(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
    2. "The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides."
      Kanter U., Usadel B., Guerineau F., Li Y., Pauly M., Tenhaken R.
      Planta 221:243-254(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION.
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiMIOX4_ARATH
    AccessioniPrimary (citable) accession number: Q8H1S0
    Secondary accession number(s): Q6XGZ9, Q9STQ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3