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Q8H1H9 (AMO_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Primary amine oxidase
EC=1.4.3.21
Alternative name(s):
Amine oxidase [copper-containing]
Gene names
Ordered Locus Names:At1g62810
ORF Names:F23N19.18
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length712 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.

Cofactor

Binds 1 copper ion per subunit By similarity.

Contains 1 topaquinone per subunit By similarity.

Subunit structure

Homodimer.

Post-translational modification

Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue By similarity.

Sequence similarities

Belongs to the copper/topaquinone oxidase family.

Sequence caution

The sequence AAF19542.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 3 genes: At1g62810, At1g62820 and At1g62830.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 712688Primary amine oxidase
PRO_0000342892

Sites

Active site3541Proton acceptor By similarity
Active site4421Schiff-base intermediate with substrate; via topaquinone By similarity
Metal binding4991Copper By similarity
Metal binding5011Copper By similarity
Metal binding5081Manganese By similarity
Metal binding5091Manganese; via carbonyl oxygen By similarity
Metal binding5101Manganese By similarity
Metal binding6511Manganese By similarity
Metal binding6521Manganese; via carbonyl oxygen By similarity
Metal binding6621Copper By similarity

Amino acid modifications

Modified residue44212',4',5'-topaquinone By similarity
Glycosylation1461N-linked (GlcNAc...) Potential
Glycosylation1731N-linked (GlcNAc...) Potential
Glycosylation5161N-linked (GlcNAc...) Potential
Glycosylation6171N-linked (GlcNAc...) Potential
Disulfide bond188 ↔ 210 By similarity
Disulfide bond373 ↔ 399 By similarity

Experimental info

Sequence conflict2011E → K in AAO42785. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8H1H9 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 451908CE887D1F64

FASTA71280,138
        10         20         30         40         50         60 
MAEPSFARLF LLFFSFLLIF ATYSWVFGPD SGFLFGTRVR KTLGSNRQVH VDHSLEKPHH 

        70         80         90        100        110        120 
PLDPLTVREI NRVRTILSNH DPGFGSGSAT IHSMALDEPE KSRVVQWKKG NKLLSRRAAV 

       130        140        150        160        170        180 
VAYWGGQTHE ITVDLDSGRV VSDVINRTSG YPILTLNDVF AASQVPLKSL EFNRSIEARG 

       190        200        210        220        230        240 
VKFSDLACIT PFAGWFGSEE EGRRVIRVQC FTLQGTTNYF MRPLEGLYVT VDLDKLEVIK 

       250        260        270        280        290        300 
IIDKGPIPIP KASGTEYRFG VQNKPVHMDR INPISMEQPD GPSFRVEDGH LVKWANWVFH 

       310        320        330        340        350        360 
VKADQRAGMI ISQATVRDSE TGEPRSVMYK GFPSELFVPY MDPEEGWYYK GYMDAGELGL 

       370        380        390        400        410        420 
GPTAMPLVPL NDCPRNSYYI DGVFASPDGK PIVQPNMICL FERYAGDISW RHSEILFANA 

       430        440        450        460        470        480 
DIRESRPKVT LVARMATSVG NYDYIFDWEF QTDGLIRVTV AASGMLMVKG TPYDNVDDLG 

       490        500        510        520        530        540 
DREDDAGPLI SENVIGVVHD HFITFHLDMD IDGPMNNSLV KVHLEKQRVP TGKSPRKSYL 

       550        560        570        580        590        600 
KVKKYIAKTE KDAQIKLSLY DPYEFHIVNP NRKSRVGNPA GYRIVPGGNA ASLLDHDDPP 

       610        620        630        640        650        660 
QIRGAFTNNQ IWVTPYNRSE QYAGGVLIYQ SQGDDTLQVW SDRDRSIENK DIVLWYTLGF 

       670        680        690        700        710 
HHVPCQEDYP VMPTVAASFE LKPANFFESN PILGSAPFFE KDLPVCRPFA SS

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC007190 Genomic DNA. Translation: AAF19542.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34008.1.
AY149440 mRNA. Translation: AAN12916.1.
BT004539 mRNA. Translation: AAO42785.1.
IPIIPI00529460.
RefSeqNP_176469.1. NM_104959.3.
UniGeneAt.36219.

3D structure databases

HSSPHSSP built from PDB template 1KSI based on UniProtKB Q43077.
ProteinModelPortalQ8H1H9.
SMRQ8H1H9. Positions 59-706.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8H1H9.

Proteomic databases

PRIDEQ8H1H9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G62810.1; AT1G62810.1; AT1G62810.
GeneID842580.
GenomeReviewsGene locus AT1G62810 in contig CT485782_GR.
KEGGath:AT1G62810.
NMPDRfig|3702.1.peg.5674.

Organism-specific databases

TAIRAt1g62810.

Phylogenomic databases

eggNOGKOG1186.
GeneTreeEPGT00050000003135.
HOGENOMHBG325455.
InParanoidQ8H1H9.
OMAINPISME.
PhylomeDBQ8H1H9.
ProtClustDBCLSN2682354.

Gene expression databases

GenevestigatorQ8H1H9.

Family and domain databases

InterProIPR000269. Cu_amine_oxidase.
IPR015798. Cu_amine_oxidase_C.
IPR016182. Cu_amine_oxidase_N-reg.
IPR015800. Cu_amine_oxidase_N2.
IPR015801. Cu_amine_oxidase_N2/3.
IPR015802. Cu_amine_oxidase_N3.
[Graphical view]
Gene3DG3DSA:3.10.450.40. CuNH_oxidase. 2 hits.
G3DSA:2.70.98.20. Lyase_8_central. 1 hit.
PANTHERPTHR10638. CuNH_oxidase. 1 hit.
PfamPF01179. Cu_amine_oxid. 1 hit.
PF02727. Cu_amine_oxidN2. 1 hit.
PF02728. Cu_amine_oxidN3. 1 hit.
[Graphical view]
SUPFAMSSF54416. Cu_amine_oxidase_N-reg. 2 hits.
SSF49998. CuNH_oxidase. 1 hit.
PROSITEPS01164. COPPER_AMINE_OXID_1. False negative.
PS01165. COPPER_AMINE_OXID_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMO_ARATH
AccessionPrimary (citable) accession number: Q8H1H9
Secondary accession number(s): Q84W14, Q9SI68
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: March 1, 2003
Last modified: September 21, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

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