Q8H1G6 (PTI11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PTI1-like tyrosine-protein kinase 1
Short name=PTI1-1
EC=2.7.10.2

Gene names

Name:	PTI11
Ordered Locus Names:	At1g06700
ORF Names:	F12K11.1, F4H5.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with OXI1. Ref.5
Subcellular location	Cell membrane; Peripheral membrane protein Ref.6 Ref.7.
Post-translational modification	Autophosphorylated and phosphorylated by OXI1. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence AAF24808.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAF63147.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	plasma membrane Inferred from direct assay Ref.6Ref.7. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	PTI1-like tyrosine-protein kinase 1		PRO_0000403322
Regions
	Domain	68 – 350	283	Protein kinase
	Nucleotide binding	74 – 82	9	ATP By similarity
Sites
	Active site	200	1	Proton acceptor By similarity
	Binding site	96	1	ATP By similarity
Experimental info
	Sequence conflict	343	1	V → A in AAK44075. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8H1G6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 49CD8A555DEF458B	FASTA	361	39,820
	10 20 30 40 50 60 MRKWICCTCQ IEDSNEEQQL KSSQQQSDAN HKNSKPAPVA KHEVKKEALP IEVPPLSLDE 70 80 90 100 110 120 VKEKTENFGS KALIGEGSYG RVYYATLNDG VAVALKKLDV APEAETDTEF LSQVSMVSRL 130 140 150 160 170 180 KHENLIQLLG FCVDGNLRVL AYEFATMGSL HDILHGRKGV QGAQPGPTLD WITRVKIAVE 190 200 210 220 230 240 AARGLEYLHE KSQPPVIHRD IRSSNVLLFE DYKAKIADFN LSNQAPDNAA RLHSTRVLGT 250 260 270 280 290 300 FGYHAPEYAM TGQLTQKSDV YSFGVVLLEL LTGRKPVDHT MPRGQQSLVT WATPRLSEDK 310 320 330 340 350 360 VKQCIDPKLK ADYPPKAVAK LAAVAALCVQ YEAEFRPNMS IVVKALQPLL KPPAAAPAPE S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. Tissue: Root.
[5]	"The Arabidopsis protein kinase PTI1-2 is activated by convergent phosphatidic acid and oxidative stress signaling pathways downstream of PDK1 and OXI1." Anthony R.G., Khan S., Costa J., Pais M.S., Boegre L. J. Biol. Chem. 281:37536-37546(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH OXI1, AUTOPHOSPHORYLATION, PHOSPHORYLATION BY OXI1.
[6]	"Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis." Benschop J.J., Mohammed S., O'Flaherty M., Heck A.J.R., Slijper M., Menke F.L.H. Mol. Cell. Proteomics 6:1198-1214(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Columbia.
[7]	"A high content in lipid-modified peripheral proteins and integral receptor kinases features in the arabidopsis plasma membrane proteome." Marmagne A., Ferro M., Meinnel T., Bruley C., Kuhn L., Garin J., Barbier-Brygoo H., Ephritikhine G. Mol. Cell. Proteomics 6:1980-1996(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Web resources

PlantP kinase Classification PPC

Arabidopsis protein tyrosine kinases

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007592 Genomic DNA. Translation: AAF24808.1. Different initiation. AC011001 Genomic DNA. Translation: AAF63147.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE28025.1. CP002684 Genomic DNA. Translation: AEE28026.1. AF370260 mRNA. Translation: AAK44075.1. AY150374 mRNA. Translation: AAN12919.1. AK317491 mRNA. Translation: BAH20156.1.
IPI	IPI00540599.
PIR	F86201.
RefSeq	NP_001030981.1. NM_001035904.1. NP_172155.1. NM_100547.2.
UniGene	At.12093.
3D structure databases
ProteinModelPortal	Q8H1G6.
SMR	Q8H1G6. Positions 15-349.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-8085735.
Proteomic databases
PaxDb	Q8H1G6.
PRIDE	Q8H1G6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G06700.1; AT1G06700.1; AT1G06700. AT1G06700.2; AT1G06700.2; AT1G06700.
GeneID	837180.
KEGG	ath:AT1G06700.
Organism-specific databases
GeneFarm	1782. 139.
TAIR	At1g06700.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000116550.
InParanoid	Q8H1G6.
OMA	TENFGSK.
PhylomeDB	Q8H1G6.
ProtClustDB	CLSN2682494.
Gene expression databases
Genevestigator	Q8H1G6.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTI11_ARATH

Accession

Primary (citable) accession number: Q8H1G6
Secondary accession number(s): Q94K67, Q9M9X8, Q9SHK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program