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Protein

Regulator of G-protein signaling 1

Gene

RGS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glucose-regulated GTPase-accelerating protein (GAP) for the GTP-bound self-activating heterotrimeric G alpha protein GPA1. Cooperates with G beta-gamma dimers to maintain an unactivated but fully functional pool of GPA1. Phosphorylation-dependent endocytosis of RGS1 physically uncouples the two proteins, resulting in signal activation. Free AGB1 is essential, but not sufficient, for RGS1 endocytosis. Modulates cell proliferation, abscisic acid (ABA) and drought stress signal transduction by acting in a hexokinase-independent glucose-signaling pathway (PubMed:26528314). Involved in the shapes of leaves, the development of floral buds, the elongation of stems, siliques, and hypocotyls, the time of flowering and the regulation of guard-cell K+ and anion channels. Important for the kinetics of voltage activation of inward K+ current but not for the current amplitude.10 Publications

GO - Molecular functioni

  • G-protein coupled receptor activity Source: TAIR
  • GTPase activator activity Source: TAIR

GO - Biological processi

  • negative regulation of signal transduction Source: UniProtKB-KW
  • positive regulation of GTPase activity Source: TAIR
  • regulation of cell proliferation Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to glucose Source: TAIR
  • response to water deprivation Source: TAIR
  • sugar mediated signaling pathway Source: TAIR

Keywordsi

Molecular functionSignal transduction inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 1
Short name:
AtRGS1
Gene namesi
Name:RGS1
Ordered Locus Names:At3g26090
ORF Names:MPE11.27, MPE11.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G26090
TAIRilocus:2092180 AT3G26090

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
Transmembranei83 – 103HelicalSequence analysisAdd BLAST21
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Transmembranei157 – 177HelicalSequence analysisAdd BLAST21
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21
Transmembranei226 – 246HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Increased cell elongation and longer hypocotyls when grown in the dark. Longer primary roots when grown in light due to increased cell production in root meristem. Loss of stratification requirement for seed germination. Decreased sensitivity to glucose and abscisic acid (ABA) in seed germination process (PubMed:26528314). No effect on ABA inhibition of stomatal opening.5 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi320E → K: Loss of GTPase-accelerating activity, loss of interaction with GPA1 and loss of endocytosis. 1 Publication1
Mutagenesisi428S → A: Loss of endocytosis; when associated with A-435 and A-436. 1 Publication1
Mutagenesisi435S → A: Loss of endocytosis; when associated with A-428 and A-436. 1 Publication1
Mutagenesisi436S → A: Loss of endocytosis; when associated with A-428 and A-435. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004212641 – 459Regulator of G-protein signaling 1Add BLAST459

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei428Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated by WNK8 and WNK10, and in vitro by WNK1. Also phosphorylated at Ser-435 or Ser-436.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8H1F2

PTM databases

iPTMnetiQ8H1F2

Expressioni

Tissue specificityi

Expressed in hypocotyls, cotyledons, guard cells and root and shoot apical meristems.3 Publications

Inductioni

Up-regulated by ozone.1 Publication

Gene expression databases

ExpressionAtlasiQ8H1F2 baseline and differential
GenevisibleiQ8H1F2 AT

Interactioni

Subunit structurei

Interacts (via C-ter) with GPA1 (PubMed:14500984, PubMed:17951432, PubMed:18817773, PubMed:21952135). Interacts with WNK1, WNK8, WNK10, SYP23. The association with WNK8 at the plasma membrane is triggered by induction of glucose and continues with trafficking into endocytic compartments (PubMed:21952135). Interacts with NDL1, NDL2 and NDL3 (PubMed:19948787). Interacts with RHIP1 (PubMed:26528314).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GPA1P180646EBI-1627025,EBI-443890

Protein-protein interaction databases

BioGridi7538, 151 interactors
IntActiQ8H1F2, 1 interactor
MINTiQ8H1F2
STRINGi3702.AT3G26090.1

Structurei

3D structure databases

ProteinModelPortaliQ8H1F2
SMRiQ8H1F2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini295 – 413RGSPROSITE-ProRule annotationAdd BLAST119

Domaini

The C-terminal domain (249-459) is able to bind to and to accelerate the GTPase activity of GPA1.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II3E Eukaryota
ENOG4111T7B LUCA
HOGENOMiHOG000006273
InParanoidiQ8H1F2
OMAiFEFQRMG
OrthoDBiEOG09360E8X
PhylomeDBiQ8H1F2

Family and domain databases

InterProiView protein in InterPro
IPR016137 RGS
IPR036305 RGS_sf
PfamiView protein in Pfam
PF00615 RGS, 1 hit
SMARTiView protein in SMART
SM00315 RGS, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
PROSITEiView protein in PROSITE
PS50132 RGS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8H1F2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGCALHGG CPSDYVAVAI SVICFFVLLS RSVLPCLIHK APRTNSSSFW
60 70 80 90 100
IPVIQVISSF NLLFSIMMSV NLLRFRTKHW WRYCYLWAVW IEGPLGFGLL
110 120 130 140 150
MSCRITQAFQ LYFIFVKKRL PPVKSYIFLP LVLLPWIFGA AIIHATKPLN
160 170 180 190 200
DKCHMGLQWT FPVAGLHALY VLALIAFTRA VRHVEFRFDE LRDLWKGILV
210 220 230 240 250
SATSIVIWVT AFVLNEIHEE ISWLQVASRF VLLVTGGILV VVFFSISSNQ
260 270 280 290 300
PLLSQISLKK RQNFEFQRMG QALGIPDSGL LFRKEEFRPV DPNEPLDKLL
310 320 330 340 350
LNKRFRHSFM EFADSCYAGE TLHFFEEVYE HGKIPEDDSI RRIYMARHIM
360 370 380 390 400
EKFIVAGAEM ELNLSHKTRQ EILTTQDLTH TDLFKNALNE VMQLIKMNLV
410 420 430 440 450
RDYWSSIYFI KFKEEESCHE AMHKEGYSFS SPRLSSVQGS DDPFYQEHMS

KSSRCSSPG
Length:459
Mass (Da):52,947
Last modified:March 1, 2003 - v1
Checksum:i43AA65CD0417488E
GO

Sequence cautioni

The sequence BAB01073 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAB01074 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti426G → R in AAM14091 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023041 Genomic DNA Translation: BAB01073.1 Sequence problems.
AB023041 Genomic DNA Translation: BAB01074.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE77116.1
AY091142 mRNA Translation: AAM14091.1
AY150429 mRNA Translation: AAN12971.1
RefSeqiNP_189238.2, NM_113514.4
UniGeneiAt.37280
At.43902

Genome annotation databases

EnsemblPlantsiAT3G26090.1; AT3G26090.1; AT3G26090
GeneIDi822207
GrameneiAT3G26090.1; AT3G26090.1; AT3G26090
KEGGiath:AT3G26090

Similar proteinsi

Entry informationi

Entry nameiRGS1_ARATH
AccessioniPrimary (citable) accession number: Q8H1F2
Secondary accession number(s): Q8RWS5, Q9LU81, Q9LU82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health