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Protein

Regulator of G-protein signaling 1

Gene

RGS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glucose-regulated GTPase-accelerating protein (GAP) for the GTP-bound self-activating heterotrimeric G alpha protein GPA1. Cooperates with G beta-gamma dimers to maintain an unactivated but fully functional pool of GPA1. Phosphorylation-dependent endocytosis of RGS1 physically uncouples the two proteins, resulting in signal activation. Free AGB1 is essential, but not sufficient, for RGS1 endocytosis. Modulates cell proliferation, abscisic acid (ABA) and drought stress signal transduction by acting in a hexokinase-independent glucose-signaling pathway. Involved in the shapes of leaves, the development of floral buds, the elongation of stems, siliques, and hypocotyls, the time of flowering and the regulation of guard-cell K+ and anion channels. Important for the kinetics of voltage activation of inward K+ current but not for the current amplitude.9 Publications

GO - Molecular functioni

  • G-protein coupled receptor activity Source: TAIR
  • GTPase activator activity Source: TAIR

GO - Biological processi

  • negative regulation of signal transduction Source: UniProtKB-KW
  • positive regulation of GTPase activity Source: TAIR
  • regulation of cell proliferation Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to glucose Source: TAIR
  • response to water deprivation Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Signal transduction inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 1
Short name:
AtRGS1
Gene namesi
Name:RGS1
Ordered Locus Names:At3g26090
ORF Names:MPE11.27, MPE11.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G26090.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Transmembranei48 – 6821HelicalSequence analysisAdd
BLAST
Transmembranei83 – 10321HelicalSequence analysisAdd
BLAST
Transmembranei126 – 14621HelicalSequence analysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence analysisAdd
BLAST
Transmembranei194 – 21421HelicalSequence analysisAdd
BLAST
Transmembranei226 – 24621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endosome membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Increased cell elongation and longer hypocotyls when grown in the dark. Longer primary roots when grown in light due to increased cell production in root meristem. Loss of stratification requirement for seed germination. Decreased sensitivity to glucose and abscisic acid (ABA) in seed germination process. No effect on ABA inhibition of stomatal opening.4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi320 – 3201E → K: Loss of GTPase-accelerating activity, loss of interaction with GPA1 and loss of endocytosis. 1 Publication
Mutagenesisi428 – 4281S → A: Loss of endocytosis; when associated with A-435 and A-436. 1 Publication
Mutagenesisi435 – 4351S → A: Loss of endocytosis; when associated with A-428 and A-436. 1 Publication
Mutagenesisi436 – 4361S → A: Loss of endocytosis; when associated with A-428 and A-435. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459Regulator of G-protein signaling 1PRO_0000421264Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei428 – 4281Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by WNK8 and WNK10, and in vitro by WNK1. Also phosphorylated at Ser-435 or Ser-436.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8H1F2.
PRIDEiQ8H1F2.

PTM databases

iPTMnetiQ8H1F2.

Expressioni

Tissue specificityi

Expressed in hypocotyls, cotyledons, guard cells and root and shoot apical meristems.3 Publications

Inductioni

Up-regulated by ozone.1 Publication

Gene expression databases

GenevisibleiQ8H1F2. AT.

Interactioni

Subunit structurei

Interacts with WNK1, WNK8, WNK10, SYP23 and (via C-ter) with GPA1. The association with WNK8 at the plasma membrane is triggered by induction of glucose and continues with trafficking into endocytic compartments.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GPA1P180646EBI-1627025,EBI-443890

Protein-protein interaction databases

BioGridi7538. 151 interactions.
IntActiQ8H1F2. 1 interaction.
MINTiMINT-6743110.
STRINGi3702.AT3G26090.1.

Structurei

3D structure databases

ProteinModelPortaliQ8H1F2.
SMRiQ8H1F2. Positions 10-115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini295 – 413119RGSPROSITE-ProRule annotationAdd
BLAST

Domaini

The C-terminal domain (249-459) is able to bind to and to accelerate the GTPase activity of GPA1.

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II3E. Eukaryota.
ENOG4111T7B. LUCA.
HOGENOMiHOG000006273.
InParanoidiQ8H1F2.
OMAiHWWQSCY.
OrthoDBiEOG09360E8X.
PhylomeDBiQ8H1F2.

Family and domain databases

InterProiIPR016137. RGS.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8H1F2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGCALHGG CPSDYVAVAI SVICFFVLLS RSVLPCLIHK APRTNSSSFW
60 70 80 90 100
IPVIQVISSF NLLFSIMMSV NLLRFRTKHW WRYCYLWAVW IEGPLGFGLL
110 120 130 140 150
MSCRITQAFQ LYFIFVKKRL PPVKSYIFLP LVLLPWIFGA AIIHATKPLN
160 170 180 190 200
DKCHMGLQWT FPVAGLHALY VLALIAFTRA VRHVEFRFDE LRDLWKGILV
210 220 230 240 250
SATSIVIWVT AFVLNEIHEE ISWLQVASRF VLLVTGGILV VVFFSISSNQ
260 270 280 290 300
PLLSQISLKK RQNFEFQRMG QALGIPDSGL LFRKEEFRPV DPNEPLDKLL
310 320 330 340 350
LNKRFRHSFM EFADSCYAGE TLHFFEEVYE HGKIPEDDSI RRIYMARHIM
360 370 380 390 400
EKFIVAGAEM ELNLSHKTRQ EILTTQDLTH TDLFKNALNE VMQLIKMNLV
410 420 430 440 450
RDYWSSIYFI KFKEEESCHE AMHKEGYSFS SPRLSSVQGS DDPFYQEHMS

KSSRCSSPG
Length:459
Mass (Da):52,947
Last modified:March 1, 2003 - v1
Checksum:i43AA65CD0417488E
GO

Sequence cautioni

The sequence BAB01073 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAB01074 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti426 – 4261G → R in AAM14091 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023041 Genomic DNA. Translation: BAB01073.1. Sequence problems.
AB023041 Genomic DNA. Translation: BAB01074.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE77116.1.
AY091142 mRNA. Translation: AAM14091.1.
AY150429 mRNA. Translation: AAN12971.1.
RefSeqiNP_189238.2. NM_113514.3.
UniGeneiAt.37280.
At.43902.

Genome annotation databases

EnsemblPlantsiAT3G26090.1; AT3G26090.1; AT3G26090.
GeneIDi822207.
GrameneiAT3G26090.1; AT3G26090.1; AT3G26090.
KEGGiath:AT3G26090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023041 Genomic DNA. Translation: BAB01073.1. Sequence problems.
AB023041 Genomic DNA. Translation: BAB01074.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE77116.1.
AY091142 mRNA. Translation: AAM14091.1.
AY150429 mRNA. Translation: AAN12971.1.
RefSeqiNP_189238.2. NM_113514.3.
UniGeneiAt.37280.
At.43902.

3D structure databases

ProteinModelPortaliQ8H1F2.
SMRiQ8H1F2. Positions 10-115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7538. 151 interactions.
IntActiQ8H1F2. 1 interaction.
MINTiMINT-6743110.
STRINGi3702.AT3G26090.1.

PTM databases

iPTMnetiQ8H1F2.

Proteomic databases

PaxDbiQ8H1F2.
PRIDEiQ8H1F2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G26090.1; AT3G26090.1; AT3G26090.
GeneIDi822207.
GrameneiAT3G26090.1; AT3G26090.1; AT3G26090.
KEGGiath:AT3G26090.

Organism-specific databases

TAIRiAT3G26090.

Phylogenomic databases

eggNOGiENOG410II3E. Eukaryota.
ENOG4111T7B. LUCA.
HOGENOMiHOG000006273.
InParanoidiQ8H1F2.
OMAiHWWQSCY.
OrthoDBiEOG09360E8X.
PhylomeDBiQ8H1F2.

Miscellaneous databases

PROiQ8H1F2.

Gene expression databases

GenevisibleiQ8H1F2. AT.

Family and domain databases

InterProiIPR016137. RGS.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGS1_ARATH
AccessioniPrimary (citable) accession number: Q8H1F2
Secondary accession number(s): Q8RWS5, Q9LU81, Q9LU82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.