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Protein

Malate dehydrogenase [NADP], chloroplastic

Gene

At5g58330

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells (Probable). Plays an essential role in the regulation of catalase activity and the accumulation of a hydrogen peroxide-dependent signal by transmitting the redox state of the chloroplast to other cell compartments (PubMed:24591715).Curated1 Publication

Catalytic activityi

(S)-malate + NADP+ = oxaloacetate + NADPH.Curated

Enzyme regulationi

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei77Activation of NADP-MDHBy similarity1
Sitei82Activation of NADP-MDHBy similarity1
Binding sitei187SubstrateBy similarity1
Binding sitei193SubstrateBy similarity1
Binding sitei200NADPBy similarity1
Binding sitei207NADPBy similarity1
Binding sitei226SubstrateBy similarity1
Binding sitei257SubstrateBy similarity1
Active sitei282Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi106 – 112NADPBy similarity7
Nucleotide bindingi224 – 226NADPBy similarity3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: GO_Central
  • malate dehydrogenase (NADP+) activity Source: InterPro
  • NAD(P)+ transhydrogenase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT5G58330-MONOMER.
BRENDAi1.1.1.40. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase [NADP], chloroplasticCurated (EC:1.1.1.82Curated)
Alternative name(s):
NADP-MDHCurated
Gene namesi
Ordered Locus Names:At5g58330Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G58330.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • mitochondrion Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions and high-light conditions in short days.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52ChloroplastSequence analysisAdd BLAST52
ChainiPRO_000043832553 – 443Malate dehydrogenase [NADP], chloroplasticAdd BLAST391

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi77 ↔ 82In oxidized inactive NADP-MDHBy similarity
Disulfide bondi418 ↔ 430In oxidized inactive NADP-MDHBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By low temperature and high light.1 Publication

Gene expression databases

ExpressionAtlasiQ8H1E2. baseline and differential.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G58330.1.

Structurei

3D structure databases

SMRiQ8H1E2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1496. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000220953.
OMAiAFSQECI.
OrthoDBiEOG09360AEK.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8H1E2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMAELSTPK TTSPFLNSSS RLRLSSKLHL SNHFRHLLLP PLHTTTPNSK
60 70 80 90 100
ISCSVSQNSQ APVAVQENGL VKTKKECYGV FCLTYDLKAE EETRSWKKLI
110 120 130 140 150
NIAVSGAAGM ISNHLLFKLA SGEVFGPDQP IALKLLGSER SIQALEGVAM
160 170 180 190 200
ELEDSLFPLL REVDIGTDPN EVFQDVEWAI LIGAKPRGPG MERADLLDIN
210 220 230 240 250
GQIFAEQGKA LNKAASPNVK VLVVGNPCNT NALICLKNAP NIPAKNFHAL
260 270 280 290 300
TRLDENRAKC QLALKAGVFY DKVSNMTIWG NHSTTQVPDF LNARINGLPV
310 320 330 340 350
KEVITDHKWL EEGFTESVQK RGGLLIQKWG RSSAASTAVS IVDAIKSLVT
360 370 380 390 400
PTPEGDWFST GVYTDGNPYG IEEGLVFSMP CRSKGDGDYE LVKDVEIDDY
410 420 430 440
LRQRIAKSEA ELLAEKRCVA HLTGEGIAYC DLGPVDTMLP GEV
Length:443
Mass (Da):48,316
Last modified:March 1, 2003 - v1
Checksum:i29BE4C23B9E94F41
GO
Isoform 2 (identifier: Q8H1E2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-59: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.Curated
Show »
Length:442
Mass (Da):48,229
Checksum:iE1C55B0DA76BC942
GO

Sequence cautioni

The sequence AAM63456 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti380P → L in AAM63456 (Ref. 5) Curated1
Sequence conflicti414A → P in AAL67025 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05865159Missing in isoform 2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019228 Genomic DNA. Translation: BAA96924.1.
CP002688 Genomic DNA. Translation: AED97037.1.
CP002688 Genomic DNA. Translation: AED97038.1.
AY074329 mRNA. Translation: AAL67025.1.
AY150479 mRNA. Translation: AAN13004.1.
AK226364 mRNA. Translation: BAE98512.1.
AY086453 mRNA. Translation: AAM63456.1. Different initiation.
RefSeqiNP_851214.1. NM_180883.3.
UniGeneiAt.71066.
At.9574.

Genome annotation databases

EnsemblPlantsiAT5G58330.1; AT5G58330.1; AT5G58330.
GeneIDi835945.
GrameneiAT5G58330.1; AT5G58330.1; AT5G58330.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019228 Genomic DNA. Translation: BAA96924.1.
CP002688 Genomic DNA. Translation: AED97037.1.
CP002688 Genomic DNA. Translation: AED97038.1.
AY074329 mRNA. Translation: AAL67025.1.
AY150479 mRNA. Translation: AAN13004.1.
AK226364 mRNA. Translation: BAE98512.1.
AY086453 mRNA. Translation: AAM63456.1. Different initiation.
RefSeqiNP_851214.1. NM_180883.3.
UniGeneiAt.71066.
At.9574.

3D structure databases

SMRiQ8H1E2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G58330.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G58330.1; AT5G58330.1; AT5G58330.
GeneIDi835945.
GrameneiAT5G58330.1; AT5G58330.1; AT5G58330.

Organism-specific databases

TAIRiAT5G58330.

Phylogenomic databases

eggNOGiKOG1496. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000220953.
OMAiAFSQECI.
OrthoDBiEOG09360AEK.

Enzyme and pathway databases

BioCyciARA:AT5G58330-MONOMER.
BRENDAi1.1.1.40. 399.

Gene expression databases

ExpressionAtlasiQ8H1E2. baseline and differential.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDHNP_ARATH
AccessioniPrimary (citable) accession number: Q8H1E2
Secondary accession number(s): Q8LCQ9, Q8VXZ3, Q9LVL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2016
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.