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Q8H1D8 (APY4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable apyrase 4

Short name=AtAPY4
EC=3.6.1.5
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name=ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 4
Gene names
Name:APY4
Ordered Locus Names:At1g14230
ORF Names:F7A19.33
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates By similarity.

Catalytic activity

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactor

Calcium By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Expressed both in the primary root and lateral root but not in the rosette leaves. Ref.1

Disruption phenotype

No visible phenotype. Ref.1

Sequence similarities

Belongs to the GDA1/CD39 NTPase family.

Sequence caution

The sequence AAD39310.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentMembrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandATP-binding
Calcium
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Probable apyrase 4
PRO_0000420342

Regions

Topological domain1 – 4545Cytoplasmic Potential
Transmembrane46 – 6621Helical; Signal-anchor for type II membrane protein; Potential
Topological domain67 – 503437Extracellular Potential
Nucleotide binding83 – 9311ATP-binding Probable
Nucleotide binding230 – 24011ATP-binding Probable

Sites

Active site2061Proton acceptor By similarity

Amino acid modifications

Glycosylation2611N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential
Glycosylation3381N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2241E → G in BAH20369. Ref.5
Sequence conflict3511Q → R in AAK76563. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8H1D8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: FE9F9E3755F7FD3F

FASTA50355,198
        10         20         30         40         50         60 
MQRSNARSRS NINSDMVDPP EVQTSPGNHR SSPSTAAKPK SKRTKSIIFV IVACVTIALG 

        70         80         90        100        110        120 
LLFIGYSILR SGRNRRVSLH YSVIIDGGSS GTRVHVFGYR IESGKPVFDF GEENYASLKL 

       130        140        150        160        170        180 
SPGLSAYADN PEGVSESVTE LVEFAKKRVH KGKLKKSDIR LMATAGMRLL ELPVQEQILD 

       190        200        210        220        230        240 
VTRRVLRSSG FDFRDEWASV ISGSDEGVYA WVVANHALGS LGGEPLKTTG IVELGGASAQ 

       250        260        270        280        290        300 
VTFVSTELVP SEFSRTLAYG NVSYNLYSHS FLDFGQDAAQ EKLSESLYNS AANSTGEGIV 

       310        320        330        340        350        360 
PDPCIPKGYI LETNLQKDLP GFLADKGKFT ATLQAAGNFS ECRSAAFAML QEEKGKCTYK 

       370        380        390        400        410        420 
RCSIGSIFTP NLQGSFLATE NFFHTSKFFG LGEKEWLSEM ILAGKRFCGE EWSKLKVKYP 

       430        440        450        460        470        480 
TFKDENLLRY CFSSAYIISM LHDSLGVALD DERIKYASKA GEEDIPLDWA LGAFILNTAT 

       490        500 
ATFDYSGKSR KILDLSNVAK YKI 

« Hide

References

« Hide 'large scale' references
[1]"Functional analyses of Arabidopsis apyrases 3 through 7."
Yang J.
Thesis (2011), University of Texas, United States
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 199-503.
Strain: cv. Columbia.
Tissue: Rosette leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JF830009 mRNA. Translation: AEJ38085.1.
AC007576 Genomic DNA. Translation: AAD39310.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29126.1.
AY045889 mRNA. Translation: AAK76563.1.
AY150499 mRNA. Translation: AAN13015.1.
AK317711 mRNA. Translation: BAH20369.1.
PIRC86276.
RefSeqNP_563942.1. NM_101289.4.
UniGeneAt.26177.

3D structure databases

ProteinModelPortalQ8H1D8.
SMRQ8H1D8. Positions 79-477.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G14230.1-P.

Proteomic databases

PRIDEQ8H1D8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G14230.1; AT1G14230.1; AT1G14230.
GeneID837984.
KEGGath:AT1G14230.

Organism-specific databases

GeneFarm3052. 285.
TAIRAT1G14230.

Phylogenomic databases

eggNOGCOG5371.
HOGENOMHOG000077482.
InParanoidQ8H1D8.
KOK01510.
OMAFANDYPL.
PhylomeDBQ8H1D8.
ProtClustDBCLSN2682157.

Enzyme and pathway databases

BioCycARA:AT1G14230-MONOMER.

Gene expression databases

GenevestigatorQ8H1D8.

Family and domain databases

InterProIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERPTHR11782. PTHR11782. 1 hit.
PfamPF01150. GDA1_CD39. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPY4_ARATH
AccessionPrimary (citable) accession number: Q8H1D8
Secondary accession number(s): B9DI02, Q94AP8, Q9XI63
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names