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Q8H1D8

- APY4_ARATH

UniProt

Q8H1D8 - APY4_ARATH

Protein

Probable apyrase 4

Gene

APY4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.By similarity

    Catalytic activityi

    A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

    Cofactori

    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei206 – 2061Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi83 – 9311ATP-bindingCuratedAdd
    BLAST
    Nucleotide bindingi230 – 24011ATP-bindingCuratedAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrolase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G14230-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable apyrase 4 (EC:3.6.1.5)
    Short name:
    AtAPY4
    Alternative name(s):
    ATP-diphosphatase
    ATP-diphosphohydrolase
    Adenosine diphosphatase
    Short name:
    ADPase
    NTPDase
    Nucleoside triphosphate diphosphohydrolase 4
    Gene namesi
    Name:APY4
    Ordered Locus Names:At1g14230
    ORF Names:F7A19.33
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G14230.

    Subcellular locationi

    Membrane By similarity; Single-pass type II membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 503503Probable apyrase 4PRO_0000420342Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi261 – 2611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi338 – 3381N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8H1D8.

    Expressioni

    Tissue specificityi

    Expressed both in the primary root and lateral root but not in the rosette leaves.1 Publication

    Gene expression databases

    GenevestigatoriQ8H1D8.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G14230.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H1D8.
    SMRiQ8H1D8. Positions 79-477.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4545CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini67 – 503437ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei46 – 6621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GDA1/CD39 NTPase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5371.
    HOGENOMiHOG000077482.
    InParanoidiQ8H1D8.
    KOiK01510.
    OMAiFANDYPL.
    PhylomeDBiQ8H1D8.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8H1D8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQRSNARSRS NINSDMVDPP EVQTSPGNHR SSPSTAAKPK SKRTKSIIFV    50
    IVACVTIALG LLFIGYSILR SGRNRRVSLH YSVIIDGGSS GTRVHVFGYR 100
    IESGKPVFDF GEENYASLKL SPGLSAYADN PEGVSESVTE LVEFAKKRVH 150
    KGKLKKSDIR LMATAGMRLL ELPVQEQILD VTRRVLRSSG FDFRDEWASV 200
    ISGSDEGVYA WVVANHALGS LGGEPLKTTG IVELGGASAQ VTFVSTELVP 250
    SEFSRTLAYG NVSYNLYSHS FLDFGQDAAQ EKLSESLYNS AANSTGEGIV 300
    PDPCIPKGYI LETNLQKDLP GFLADKGKFT ATLQAAGNFS ECRSAAFAML 350
    QEEKGKCTYK RCSIGSIFTP NLQGSFLATE NFFHTSKFFG LGEKEWLSEM 400
    ILAGKRFCGE EWSKLKVKYP TFKDENLLRY CFSSAYIISM LHDSLGVALD 450
    DERIKYASKA GEEDIPLDWA LGAFILNTAT ATFDYSGKSR KILDLSNVAK 500
    YKI 503
    Length:503
    Mass (Da):55,198
    Last modified:March 1, 2003 - v1
    Checksum:iFE9F9E3755F7FD3F
    GO

    Sequence cautioni

    The sequence AAD39310.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti224 – 2241E → G in BAH20369. (PubMed:19423640)Curated
    Sequence conflicti351 – 3511Q → R in AAK76563. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF830009 mRNA. Translation: AEJ38085.1.
    AC007576 Genomic DNA. Translation: AAD39310.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29126.1.
    AY045889 mRNA. Translation: AAK76563.1.
    AY150499 mRNA. Translation: AAN13015.1.
    AK317711 mRNA. Translation: BAH20369.1.
    PIRiC86276.
    RefSeqiNP_563942.1. NM_101289.4.
    UniGeneiAt.26177.

    Genome annotation databases

    EnsemblPlantsiAT1G14230.1; AT1G14230.1; AT1G14230.
    GeneIDi837984.
    KEGGiath:AT1G14230.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF830009 mRNA. Translation: AEJ38085.1 .
    AC007576 Genomic DNA. Translation: AAD39310.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29126.1 .
    AY045889 mRNA. Translation: AAK76563.1 .
    AY150499 mRNA. Translation: AAN13015.1 .
    AK317711 mRNA. Translation: BAH20369.1 .
    PIRi C86276.
    RefSeqi NP_563942.1. NM_101289.4.
    UniGenei At.26177.

    3D structure databases

    ProteinModelPortali Q8H1D8.
    SMRi Q8H1D8. Positions 79-477.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G14230.1-P.

    Proteomic databases

    PRIDEi Q8H1D8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G14230.1 ; AT1G14230.1 ; AT1G14230 .
    GeneIDi 837984.
    KEGGi ath:AT1G14230.

    Organism-specific databases

    GeneFarmi 3052. 285.
    TAIRi AT1G14230.

    Phylogenomic databases

    eggNOGi COG5371.
    HOGENOMi HOG000077482.
    InParanoidi Q8H1D8.
    KOi K01510.
    OMAi FANDYPL.
    PhylomeDBi Q8H1D8.

    Enzyme and pathway databases

    BioCyci ARA:AT1G14230-MONOMER.

    Gene expression databases

    Genevestigatori Q8H1D8.

    Family and domain databases

    InterProi IPR000407. GDA1_CD39_NTPase.
    [Graphical view ]
    PANTHERi PTHR11782. PTHR11782. 1 hit.
    Pfami PF01150. GDA1_CD39. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional analyses of Arabidopsis apyrases 3 through 7."
      Yang J.
      Thesis (2011), University of Texas, United States
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 199-503.
      Strain: cv. Columbia.
      Tissue: Rosette leaf.

    Entry informationi

    Entry nameiAPY4_ARATH
    AccessioniPrimary (citable) accession number: Q8H1D8
    Secondary accession number(s): B9DI02, Q94AP8, Q9XI63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3