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Q8H1D8

- APY4_ARATH

UniProt

Q8H1D8 - APY4_ARATH

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Protein
Probable apyrase 4
Gene
APY4, At1g14230, F7A19.33
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates By similarity.

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Calcium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei206 – 2061Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi83 – 9311ATP-binding Inferred
Add
BLAST
Nucleotide bindingi230 – 24011ATP-binding Inferred
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G14230-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable apyrase 4 (EC:3.6.1.5)
Short name:
AtAPY4
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 4
Gene namesi
Name:APY4
Ordered Locus Names:At1g14230
ORF Names:F7A19.33
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G14230.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei46 – 6621Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini67 – 503437Extracellular Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Probable apyrase 4
PRO_0000420342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi261 – 2611N-linked (GlcNAc...) Reviewed prediction
Glycosylationi293 – 2931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi338 – 3381N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8H1D8.

Expressioni

Tissue specificityi

Expressed both in the primary root and lateral root but not in the rosette leaves.1 Publication

Gene expression databases

GenevestigatoriQ8H1D8.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G14230.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8H1D8.
SMRiQ8H1D8. Positions 79-477.

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000077482.
InParanoidiQ8H1D8.
KOiK01510.
OMAiFANDYPL.
PhylomeDBiQ8H1D8.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8H1D8-1 [UniParc]FASTAAdd to Basket

« Hide

MQRSNARSRS NINSDMVDPP EVQTSPGNHR SSPSTAAKPK SKRTKSIIFV    50
IVACVTIALG LLFIGYSILR SGRNRRVSLH YSVIIDGGSS GTRVHVFGYR 100
IESGKPVFDF GEENYASLKL SPGLSAYADN PEGVSESVTE LVEFAKKRVH 150
KGKLKKSDIR LMATAGMRLL ELPVQEQILD VTRRVLRSSG FDFRDEWASV 200
ISGSDEGVYA WVVANHALGS LGGEPLKTTG IVELGGASAQ VTFVSTELVP 250
SEFSRTLAYG NVSYNLYSHS FLDFGQDAAQ EKLSESLYNS AANSTGEGIV 300
PDPCIPKGYI LETNLQKDLP GFLADKGKFT ATLQAAGNFS ECRSAAFAML 350
QEEKGKCTYK RCSIGSIFTP NLQGSFLATE NFFHTSKFFG LGEKEWLSEM 400
ILAGKRFCGE EWSKLKVKYP TFKDENLLRY CFSSAYIISM LHDSLGVALD 450
DERIKYASKA GEEDIPLDWA LGAFILNTAT ATFDYSGKSR KILDLSNVAK 500
YKI 503
Length:503
Mass (Da):55,198
Last modified:March 1, 2003 - v1
Checksum:iFE9F9E3755F7FD3F
GO

Sequence cautioni

The sequence AAD39310.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti224 – 2241E → G in BAH20369. 1 Publication
Sequence conflicti351 – 3511Q → R in AAK76563. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JF830009 mRNA. Translation: AEJ38085.1.
AC007576 Genomic DNA. Translation: AAD39310.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29126.1.
AY045889 mRNA. Translation: AAK76563.1.
AY150499 mRNA. Translation: AAN13015.1.
AK317711 mRNA. Translation: BAH20369.1.
PIRiC86276.
RefSeqiNP_563942.1. NM_101289.4.
UniGeneiAt.26177.

Genome annotation databases

EnsemblPlantsiAT1G14230.1; AT1G14230.1; AT1G14230.
GeneIDi837984.
KEGGiath:AT1G14230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JF830009 mRNA. Translation: AEJ38085.1 .
AC007576 Genomic DNA. Translation: AAD39310.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE29126.1 .
AY045889 mRNA. Translation: AAK76563.1 .
AY150499 mRNA. Translation: AAN13015.1 .
AK317711 mRNA. Translation: BAH20369.1 .
PIRi C86276.
RefSeqi NP_563942.1. NM_101289.4.
UniGenei At.26177.

3D structure databases

ProteinModelPortali Q8H1D8.
SMRi Q8H1D8. Positions 79-477.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G14230.1-P.

Proteomic databases

PRIDEi Q8H1D8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G14230.1 ; AT1G14230.1 ; AT1G14230 .
GeneIDi 837984.
KEGGi ath:AT1G14230.

Organism-specific databases

GeneFarmi 3052. 285.
TAIRi AT1G14230.

Phylogenomic databases

eggNOGi COG5371.
HOGENOMi HOG000077482.
InParanoidi Q8H1D8.
KOi K01510.
OMAi FANDYPL.
PhylomeDBi Q8H1D8.

Enzyme and pathway databases

BioCyci ARA:AT1G14230-MONOMER.

Gene expression databases

Genevestigatori Q8H1D8.

Family and domain databases

InterProi IPR000407. GDA1_CD39_NTPase.
[Graphical view ]
PANTHERi PTHR11782. PTHR11782. 1 hit.
Pfami PF01150. GDA1_CD39. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional analyses of Arabidopsis apyrases 3 through 7."
    Yang J.
    Thesis (2011), University of Texas, United States
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 199-503.
    Strain: cv. Columbia.
    Tissue: Rosette leaf.

Entry informationi

Entry nameiAPY4_ARATH
AccessioniPrimary (citable) accession number: Q8H1D8
Secondary accession number(s): B9DI02, Q94AP8, Q9XI63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi