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Protein

Probable apyrase 4

Gene

APY4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.By similarity

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei206 – 2061Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi83 – 9311ATP-bindingCuratedAdd
BLAST
Nucleotide bindingi230 – 24011ATP-bindingCuratedAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G14230-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable apyrase 4 (EC:3.6.1.5)
Short name:
AtAPY4
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 4
Gene namesi
Name:APY4
Ordered Locus Names:At1g14230
ORF Names:F7A19.33
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G14230.

Subcellular locationi

Membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei46 – 6621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini67 – 503437ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Probable apyrase 4PRO_0000420342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi261 – 2611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi338 – 3381N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8H1D8.

Expressioni

Tissue specificityi

Expressed both in the primary root and lateral root but not in the rosette leaves.1 Publication

Gene expression databases

GenevestigatoriQ8H1D8.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G14230.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8H1D8.
SMRiQ8H1D8. Positions 79-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000077482.
InParanoidiQ8H1D8.
KOiK01510.
OMAiCSIGSIF.
PhylomeDBiQ8H1D8.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8H1D8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRSNARSRS NINSDMVDPP EVQTSPGNHR SSPSTAAKPK SKRTKSIIFV
60 70 80 90 100
IVACVTIALG LLFIGYSILR SGRNRRVSLH YSVIIDGGSS GTRVHVFGYR
110 120 130 140 150
IESGKPVFDF GEENYASLKL SPGLSAYADN PEGVSESVTE LVEFAKKRVH
160 170 180 190 200
KGKLKKSDIR LMATAGMRLL ELPVQEQILD VTRRVLRSSG FDFRDEWASV
210 220 230 240 250
ISGSDEGVYA WVVANHALGS LGGEPLKTTG IVELGGASAQ VTFVSTELVP
260 270 280 290 300
SEFSRTLAYG NVSYNLYSHS FLDFGQDAAQ EKLSESLYNS AANSTGEGIV
310 320 330 340 350
PDPCIPKGYI LETNLQKDLP GFLADKGKFT ATLQAAGNFS ECRSAAFAML
360 370 380 390 400
QEEKGKCTYK RCSIGSIFTP NLQGSFLATE NFFHTSKFFG LGEKEWLSEM
410 420 430 440 450
ILAGKRFCGE EWSKLKVKYP TFKDENLLRY CFSSAYIISM LHDSLGVALD
460 470 480 490 500
DERIKYASKA GEEDIPLDWA LGAFILNTAT ATFDYSGKSR KILDLSNVAK

YKI
Length:503
Mass (Da):55,198
Last modified:February 28, 2003 - v1
Checksum:iFE9F9E3755F7FD3F
GO

Sequence cautioni

The sequence AAD39310.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti224 – 2241E → G in BAH20369 (PubMed:19423640).Curated
Sequence conflicti351 – 3511Q → R in AAK76563 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF830009 mRNA. Translation: AEJ38085.1.
AC007576 Genomic DNA. Translation: AAD39310.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29126.1.
AY045889 mRNA. Translation: AAK76563.1.
AY150499 mRNA. Translation: AAN13015.1.
AK317711 mRNA. Translation: BAH20369.1.
PIRiC86276.
RefSeqiNP_563942.1. NM_101289.4.
UniGeneiAt.26177.

Genome annotation databases

EnsemblPlantsiAT1G14230.1; AT1G14230.1; AT1G14230.
GeneIDi837984.
KEGGiath:AT1G14230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF830009 mRNA. Translation: AEJ38085.1.
AC007576 Genomic DNA. Translation: AAD39310.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29126.1.
AY045889 mRNA. Translation: AAK76563.1.
AY150499 mRNA. Translation: AAN13015.1.
AK317711 mRNA. Translation: BAH20369.1.
PIRiC86276.
RefSeqiNP_563942.1. NM_101289.4.
UniGeneiAt.26177.

3D structure databases

ProteinModelPortaliQ8H1D8.
SMRiQ8H1D8. Positions 79-477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G14230.1-P.

Proteomic databases

PRIDEiQ8H1D8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G14230.1; AT1G14230.1; AT1G14230.
GeneIDi837984.
KEGGiath:AT1G14230.

Organism-specific databases

GeneFarmi3052. 285.
TAIRiAT1G14230.

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000077482.
InParanoidiQ8H1D8.
KOiK01510.
OMAiCSIGSIF.
PhylomeDBiQ8H1D8.

Enzyme and pathway databases

BioCyciARA:AT1G14230-MONOMER.

Gene expression databases

GenevestigatoriQ8H1D8.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional analyses of Arabidopsis apyrases 3 through 7."
    Yang J.
    Thesis (2010), University of Texas, United States
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 199-503.
    Strain: cv. Columbia.
    Tissue: Rosette leaf.

Entry informationi

Entry nameiAPY4_ARATH
AccessioniPrimary (citable) accession number: Q8H1D8
Secondary accession number(s): B9DI02, Q94AP8, Q9XI63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 27, 2012
Last sequence update: February 28, 2003
Last modified: January 6, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.