Q8H1D6 (RBK1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Receptor-like cytosolic serine/threonine-protein kinase RBK1
EC=2.7.11.1

Alternative name(s):
Protein ROP BINDING PROTEIN KINASES 1

Gene names

Name:	RBK1
Ordered Locus Names:	At5g10520
ORF Names:	F12B17.130

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with ARAC5 and ARAC10. Ref.4
Subcellular location	Cytoplasm. Endomembrane system; Peripheral membrane protein. Nucleus Ref.4.
Tissue specificity	Mostly expressed in vasculature, hydathode endothem, leaf mesophyll cells and trichomes. Ref.4
Induction	By fungal pathogens such as Phytophthora infestans and Botrytis cinerea. Promoted by ethylene in the vascular cylinder of primary roots and in the root cortex in the root/hypocotyl junction zone. Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence CAB89391.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to ethylene stimulus Inferred from expression pattern Ref.4. Source: UniProtKB defense response, incompatible interaction Inferred from expression pattern Ref.4. Source: TAIR response to parasitic fungus Inferred from expression pattern Ref.4. Source: UniProtKB
Cellular component	cytosol Inferred from direct assay Ref.4. Source: TAIR endomembrane system Inferred from direct assay Ref.4. Source: UniProtKB membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay Ref.4. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase binding Inferred from physical interaction Ref.4. Source: UniProtKB protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Receptor-like cytosolic serine/threonine-protein kinase RBK1		PRO_0000403330
Regions
	Domain	153 – 430	278	Protein kinase
	Nucleotide binding	159 – 167	9	ATP By similarity
	Compositional bias	29 – 75	47	Ser-rich
Sites
	Active site	278	1	Proton acceptor By similarity
	Binding site	181	1	ATP By similarity
Experimental info
	Sequence conflict	56	1	D → N in AAL07235. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8H1D6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E4610F132762F620	FASTA	467	52,652
	10 20 30 40 50 60 MAVEDNKNSE SKNHQEVELH RNDLGLEDSS SPRGVLGMVS DSDNSSSSCS SCSSDDKSSS 70 80 90 100 110 120 TSSPFSNTTK TVSSSHHGLQ WNKMIESIKK KSMRRFSVIP LLASYELTRK NLRRKQPKLT 130 140 150 160 170 180 PSESAFTCEA FFMAKPSWRN FTYEELAVAT DYFNPENMIG KGGHAEVYKG VLINGETVAI 190 200 210 220 230 240 KKLMSHAKEE EERVSDFLSE LGIIAHVNHP NAARLRGFSS DRGLHFVLEY APYGSLASML 250 260 270 280 290 300 FGSEECLEWK IRYKVALGIA DGLSYLHNAC PRRIIHRDIK ASNILLNHDY EAQISDFGLA 310 320 330 340 350 360 KWLPENWPHH VVFPIEGTFG YLAPEYFMHG IVDEKIDVFA FGVLLLEIIT SRRAVDTASR 370 380 390 400 410 420 QSIVAWAKPF LEKNSMEDIV DPRLGNMFNP TEMQRVMLTA SMCVHHIAAM RPDMTRLVQL 430 440 450 460 LRGEDGPAEL QQKAGERTMS VNACDLQDHT SSSYLNELRR HRQLLME

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"A cysteine-rich receptor-like kinase NCRK and a pathogen-induced protein kinase RBK1 are Rop GTPase interactors." Molendijk A.J., Ruperti B., Singh M.K., Dovzhenko A., Ditengou F.A., Milia M., Westphal L., Rosahl S., Soellick T.R., Uhrig J., Weingarten L., Huber M., Palme K. Plant J. 53:909-923(2008) [PubMed: 18088316] [Abstract] Cited for: INTERACTION WITH ARAC5 AND ARAC10, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY PATHOGENS AND ETHYLENE.
+	Additional computationally mapped references.

Web resources

PlantP kinase Classification PPC

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL353995 Genomic DNA. Translation: CAB89391.1. Sequence problems. CP002688 Genomic DNA. Translation: AED91559.1. AY056156 mRNA. Translation: AAL07235.1. AY150507 mRNA. Translation: AAN13023.1.
IPI	IPI00544114.
PIR	T49987.
RefSeq	NP_568231.1. NM_121090.3.
UniGene	At.26371.
3D structure databases
ProteinModelPortal	Q8H1D6.
SMR	Q8H1D6. Positions 137-423.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8H1D6. 8 interactions.
Proteomic databases
PRIDE	Q8H1D6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G10520.1; AT5G10520.1; AT5G10520.
GeneID	830917.
GenomeReviews	Gene locus AT5G10520 in contig BA000015_GR.
KEGG	ath:AT5G10520.
NMPDR	fig|3702.1.peg.23182.
Organism-specific databases
GeneFarm	1736. 127.
TAIR	At5g10520.
Phylogenomic databases
eggNOG	KOG1187.
HOGENOM	HBG755340.
InParanoid	Q8H1D6.
OMA	SHAKEEE.
PhylomeDB	Q8H1D6.
ProtClustDB	CLSN2717136.
Gene expression databases
Genevestigator	Q8H1D6.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RBK1_ARATH

Accession

Primary (citable) accession number: Q8H1D6
Secondary accession number(s): Q93ZZ8, Q9LXA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	March 1, 2003
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program