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Q8H1B3

- MD37B_ARATH

UniProt

Q8H1B3 - MD37B_ARATH

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Protein

Probable mediator of RNA polymerase II transcription subunit 37b

Gene

MED37B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.By similarity
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. pollen tube growth Source: TAIR
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1557-MONOMER.
ARA:GQT-820-MONOMER.
ReactomeiREACT_108190. ATF6-alpha activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mediator of RNA polymerase II transcription subunit 37b
Alternative name(s):
BiP chaperone BIP-L
Heat shock 70 kDa protein 13
Heat shock protein 70-13
Short name:
AtHsp70-13
Hsp70 protein BiP chaperone BIP-L
Luminal-binding protein 3
Short name:
AtBP3
Short name:
BiP3
Gene namesi
Name:MED37B
Synonyms:BIP-L, BIP3, HSP70-13, MED37_1
Ordered Locus Names:At1g09080
ORF Names:F7G19.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G09080.

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotation. Nucleus Curated

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence AnalysisAdd
BLAST
Chaini36 – 675640Probable mediator of RNA polymerase II transcription subunit 37bPRO_0000415430Add
BLAST

Proteomic databases

PRIDEiQ8H1B3.

Expressioni

Inductioni

By tunicamycin and by DTT.2 Publications

Gene expression databases

GenevestigatoriQ8H1B3.

Interactioni

Subunit structurei

Component of the Mediator complex.1 Publication

Protein-protein interaction databases

IntActiQ8H1B3. 2 interactions.
MINTiMINT-7712705.
STRINGi3702.AT1G09080.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8H1B3.
SMRiQ8H1B3. Positions 49-654.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi673 – 6753Prevents secretion from ERPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi557 – 665109Glu-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0443.
HOGENOMiHOG000228135.
InParanoidiQ8H1B3.
KOiK09490.
OMAiRDIKFLP.
PhylomeDBiQ8H1B3.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8H1B3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIFIKENTAK MTRNKAIACL VFLTVLDFLM NIGAALMSSL AIEGEEQKLG
60 70 80 90 100
TVIGIDLGTT YSCVGVYHNK HVEIIANDQG NRITPSWVAF TDTERLIGEA
110 120 130 140 150
AKNQAAKNPE RTIFDPKRLI GRKFDDPDVQ RDIKFLPYKV VNKDGKPYIQ
160 170 180 190 200
VKVKGEEKLF SPEEISAMIL TKMKETAEAF LGKKIKDAVI TVPAYFNDAQ
210 220 230 240 250
RQATKDAGAI AGLNVVRIIN EPTGAAIAYG LDKKGGESNI LVYDLGGGTF
260 270 280 290 300
DVSILTIDNG VFEVLSTSGD THLGGEDFDH RVMDYFIKLV KKKYNKDISK
310 320 330 340 350
DHKALGKLRR ECELAKRSLS NQHQVRVEIE SLFDGVDFSE PLTRARFEEL
360 370 380 390 400
NMDLFKKTME PVKKALKDAG LKKSDIDEIV LVGGSTRIPK VQQMLKDFFD
410 420 430 440 450
GKEPSKGTNP DEAVAYGAAV QGGVLSGEGG EETQNILLLD VAPLSLGIET
460 470 480 490 500
VGGVMTNIIP RNTVIPTKKS QVFTTYQDQQ TTVTINVYEG ERSMTKDNRE
510 520 530 540 550
LGKFDLTGIL PAPRGVPQIE VTFEVDANGI LQVKAEDKVA KTSQSITITN
560 570 580 590 600
DKGRLTEEEI EEMIREAEEF AEEDKIMKEK IDARNKLETY VYNMKSTVAD
610 620 630 640 650
KEKLAKKISD EDKEKMEGVL KEALEWLEEN VNAEKEDYDE KLKEVELVCD
660 670
PVIKSVYEKT EGENEDDDGD DHDEL
Length:675
Mass (Da):75,149
Last modified:March 1, 2003 - v1
Checksum:i6FF9FAE4B2A4702D
GO
Isoform 2 (identifier: Q8H1B3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     25-34: Missing.

Note: Constructed according to the conserved gene model. No experimental confirmation available.

Show »
Length:665
Mass (Da):74,075
Checksum:iA36094C0C933358E
GO

Sequence cautioni

The sequence AAB70400.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei25 – 3410Missing in isoform 2. CuratedVSP_042244

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY156728 mRNA. Translation: AAN60163.1.
AC000106 Genomic DNA. Translation: AAB70400.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28392.1.
CP002684 Genomic DNA. Translation: AEE28393.1.
PIRiH86222.
RefSeqiNP_001184944.1. NM_001198015.1. [Q8H1B3-2]
NP_172382.4. NM_100779.5. [Q8H1B3-1]
UniGeneiAt.42242.

Genome annotation databases

EnsemblPlantsiAT1G09080.1; AT1G09080.1; AT1G09080. [Q8H1B3-1]
GeneIDi837429.
KEGGiath:AT1G09080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY156728 mRNA. Translation: AAN60163.1 .
AC000106 Genomic DNA. Translation: AAB70400.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE28392.1 .
CP002684 Genomic DNA. Translation: AEE28393.1 .
PIRi H86222.
RefSeqi NP_001184944.1. NM_001198015.1. [Q8H1B3-2 ]
NP_172382.4. NM_100779.5. [Q8H1B3-1 ]
UniGenei At.42242.

3D structure databases

ProteinModelPortali Q8H1B3.
SMRi Q8H1B3. Positions 49-654.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8H1B3. 2 interactions.
MINTi MINT-7712705.
STRINGi 3702.AT1G09080.1-P.

Proteomic databases

PRIDEi Q8H1B3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G09080.1 ; AT1G09080.1 ; AT1G09080 . [Q8H1B3-1 ]
GeneIDi 837429.
KEGGi ath:AT1G09080.

Organism-specific databases

TAIRi AT1G09080.

Phylogenomic databases

eggNOGi COG0443.
HOGENOMi HOG000228135.
InParanoidi Q8H1B3.
KOi K09490.
OMAi RDIKFLP.
PhylomeDBi Q8H1B3.

Enzyme and pathway databases

BioCyci ARA:GQT-1557-MONOMER.
ARA:GQT-820-MONOMER.
Reactomei REACT_108190. ATF6-alpha activates chaperone genes.

Gene expression databases

Genevestigatori Q8H1B3.

Family and domain databases

Gene3Di 1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProi IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view ]
Pfami PF00012. HSP70. 1 hit.
[Graphical view ]
PRINTSi PR00301. HEATSHOCK70.
SUPFAMi SSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of an evolutionarily distinct novel BiP gene during the unfolded protein response in Arabidopsis thaliana."
    Noh S.J., Kwon C.S., Oh D.H., Moon J.S., Chung W.I.
    Gene 311:81-91(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana."
    Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M.
    Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family."
    Sung D.Y., Vierling E., Guy C.L.
    Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNAK GENE SUBFAMILY.
  6. "Heat induces the splicing by IRE1 of a mRNA encoding a transcription factor involved in the unfolded protein response in Arabidopsis."
    Deng Y., Humbert S., Liu J.X., Srivastava R., Rothstein S.J., Howell S.H.
    Proc. Natl. Acad. Sci. U.S.A. 108:7247-7252(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY DTT.
  7. "The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress."
    Mathur S., Vyas S., Kapoor S., Tyagi A.K.
    Plant Physiol. 157:1609-1627(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SUBUNIT, NOMENCLATURE.

Entry informationi

Entry nameiMD37B_ARATH
AccessioniPrimary (citable) accession number: Q8H1B3
Secondary accession number(s): F4HZD5, O04022
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3