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Q8H1B3 (MD37B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mediator of RNA polymerase II transcription subunit 37b
Alternative name(s):
BiP chaperone BIP-L
Heat shock 70 kDa protein 13
Heat shock protein 70-13
Short name=AtHsp70-13
Hsp70 protein BiP chaperone BIP-L
Luminal-binding protein 3
Short name=AtBP3
Short name=BiP3
Gene names
Name:MED37B
Synonyms:BIP-L, BIP3, HSP70-13, MED37_1
Ordered Locus Names:At1g09080
ORF Names:F7G19.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length675 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER By similarity.

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage By similarity.

Subunit structure

Component of the Mediator complex Probable. Ref.7

Subcellular location

Endoplasmic reticulum lumen By similarity. Nucleus Probable.

Induction

By tunicamycin and by DTT. Ref.1 Ref.6

Sequence similarities

Belongs to the heat shock protein 70 (TC 1.A.33) family. DnaK subfamily. [View classification]

Sequence caution

The sequence AAB70400.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentEndoplasmic reticulum
Nucleus
   Coding sequence diversityAlternative splicing
   DomainSignal
   LigandATP-binding
Nucleotide-binding
   Molecular functionChaperone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpollen tube growth

Inferred from expression pattern PubMed 19714218. Source: TAIR

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8H1B3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8H1B3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     25-34: Missing.
Note: Constructed according to the conserved gene model. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535 Potential
Chain36 – 675640Probable mediator of RNA polymerase II transcription subunit 37b
PRO_0000415430

Regions

Motif673 – 6753Prevents secretion from ER Potential
Compositional bias557 – 665109Glu-rich

Natural variations

Alternative sequence25 – 3410Missing in isoform 2.
VSP_042244

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 6FF9FAE4B2A4702D

FASTA67575,149
        10         20         30         40         50         60 
MIFIKENTAK MTRNKAIACL VFLTVLDFLM NIGAALMSSL AIEGEEQKLG TVIGIDLGTT 

        70         80         90        100        110        120 
YSCVGVYHNK HVEIIANDQG NRITPSWVAF TDTERLIGEA AKNQAAKNPE RTIFDPKRLI 

       130        140        150        160        170        180 
GRKFDDPDVQ RDIKFLPYKV VNKDGKPYIQ VKVKGEEKLF SPEEISAMIL TKMKETAEAF 

       190        200        210        220        230        240 
LGKKIKDAVI TVPAYFNDAQ RQATKDAGAI AGLNVVRIIN EPTGAAIAYG LDKKGGESNI 

       250        260        270        280        290        300 
LVYDLGGGTF DVSILTIDNG VFEVLSTSGD THLGGEDFDH RVMDYFIKLV KKKYNKDISK 

       310        320        330        340        350        360 
DHKALGKLRR ECELAKRSLS NQHQVRVEIE SLFDGVDFSE PLTRARFEEL NMDLFKKTME 

       370        380        390        400        410        420 
PVKKALKDAG LKKSDIDEIV LVGGSTRIPK VQQMLKDFFD GKEPSKGTNP DEAVAYGAAV 

       430        440        450        460        470        480 
QGGVLSGEGG EETQNILLLD VAPLSLGIET VGGVMTNIIP RNTVIPTKKS QVFTTYQDQQ 

       490        500        510        520        530        540 
TTVTINVYEG ERSMTKDNRE LGKFDLTGIL PAPRGVPQIE VTFEVDANGI LQVKAEDKVA 

       550        560        570        580        590        600 
KTSQSITITN DKGRLTEEEI EEMIREAEEF AEEDKIMKEK IDARNKLETY VYNMKSTVAD 

       610        620        630        640        650        660 
KEKLAKKISD EDKEKMEGVL KEALEWLEEN VNAEKEDYDE KLKEVELVCD PVIKSVYEKT 

       670 
EGENEDDDGD DHDEL 

« Hide

Isoform 2 [UniParc].

Checksum: A36094C0C933358E
Show »

FASTA66574,075

References

« Hide 'large scale' references
[1]"Expression of an evolutionarily distinct novel BiP gene during the unfolded protein response in Arabidopsis thaliana."
Noh S.J., Kwon C.S., Oh D.H., Moon J.S., Chung W.I.
Gene 311:81-91(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana."
Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M.
Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family."
Sung D.Y., Vierling E., Guy C.L.
Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: DNAK GENE SUBFAMILY.
[6]"Heat induces the splicing by IRE1 of a mRNA encoding a transcription factor involved in the unfolded protein response in Arabidopsis."
Deng Y., Humbert S., Liu J.X., Srivastava R., Rothstein S.J., Howell S.H.
Proc. Natl. Acad. Sci. U.S.A. 108:7247-7252(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY DTT.
[7]"The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress."
Mathur S., Vyas S., Kapoor S., Tyagi A.K.
Plant Physiol. 157:1609-1627(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, SUBUNIT, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY156728 mRNA. Translation: AAN60163.1.
AC000106 Genomic DNA. Translation: AAB70400.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28392.1.
CP002684 Genomic DNA. Translation: AEE28393.1.
PIRH86222.
RefSeqNP_001184944.1. NM_001198015.1. [Q8H1B3-2]
NP_172382.4. NM_100779.5. [Q8H1B3-1]
UniGeneAt.42242.

3D structure databases

ProteinModelPortalQ8H1B3.
SMRQ8H1B3. Positions 49-654.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8H1B3. 2 interactions.
MINTMINT-7712705.
STRING3702.AT1G09080.1-P.

Proteomic databases

PRIDEQ8H1B3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G09080.1; AT1G09080.1; AT1G09080. [Q8H1B3-1]
GeneID837429.
KEGGath:AT1G09080.

Organism-specific databases

TAIRAT1G09080.

Phylogenomic databases

eggNOGCOG0443.
HOGENOMHOG000228135.
InParanoidQ8H1B3.
KOK09490.
OMARDIKFLP.
PhylomeDBQ8H1B3.

Enzyme and pathway databases

BioCycARA:GQT-1557-MONOMER.
ARA:GQT-820-MONOMER.

Gene expression databases

GenevestigatorQ8H1B3.

Family and domain databases

Gene3D1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSPR00301. HEATSHOCK70.
SUPFAMSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMD37B_ARATH
AccessionPrimary (citable) accession number: Q8H1B3
Secondary accession number(s): F4HZD5, O04022
Entry history
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names