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Q8H1B3

- MD37B_ARATH

UniProt

Q8H1B3 - MD37B_ARATH

Protein

Probable mediator of RNA polymerase II transcription subunit 37b

Gene

MED37B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.By similarity
    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.By similarity
    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. pollen tube growth Source: TAIR
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-1557-MONOMER.
    ARA:GQT-820-MONOMER.
    ReactomeiREACT_108190. ATF6-alpha activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable mediator of RNA polymerase II transcription subunit 37b
    Alternative name(s):
    BiP chaperone BIP-L
    Heat shock 70 kDa protein 13
    Heat shock protein 70-13
    Short name:
    AtHsp70-13
    Hsp70 protein BiP chaperone BIP-L
    Luminal-binding protein 3
    Short name:
    AtBP3
    Short name:
    BiP3
    Gene namesi
    Name:MED37B
    Synonyms:BIP-L, BIP3, HSP70-13, MED37_1
    Ordered Locus Names:At1g09080
    ORF Names:F7G19.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G09080.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation. Nucleus Curated

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3535Sequence AnalysisAdd
    BLAST
    Chaini36 – 675640Probable mediator of RNA polymerase II transcription subunit 37bPRO_0000415430Add
    BLAST

    Proteomic databases

    PRIDEiQ8H1B3.

    Expressioni

    Inductioni

    By tunicamycin and by DTT.2 Publications

    Gene expression databases

    GenevestigatoriQ8H1B3.

    Interactioni

    Subunit structurei

    Component of the Mediator complex.1 Publication

    Protein-protein interaction databases

    IntActiQ8H1B3. 2 interactions.
    MINTiMINT-7712705.
    STRINGi3702.AT1G09080.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H1B3.
    SMRiQ8H1B3. Positions 49-654.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi673 – 6753Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi557 – 665109Glu-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0443.
    HOGENOMiHOG000228135.
    InParanoidiQ8H1B3.
    KOiK09490.
    OMAiRDIKFLP.
    PhylomeDBiQ8H1B3.

    Family and domain databases

    Gene3Di1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProiIPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view]
    PfamiPF00012. HSP70. 1 hit.
    [Graphical view]
    PRINTSiPR00301. HEATSHOCK70.
    SUPFAMiSSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8H1B3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIFIKENTAK MTRNKAIACL VFLTVLDFLM NIGAALMSSL AIEGEEQKLG    50
    TVIGIDLGTT YSCVGVYHNK HVEIIANDQG NRITPSWVAF TDTERLIGEA 100
    AKNQAAKNPE RTIFDPKRLI GRKFDDPDVQ RDIKFLPYKV VNKDGKPYIQ 150
    VKVKGEEKLF SPEEISAMIL TKMKETAEAF LGKKIKDAVI TVPAYFNDAQ 200
    RQATKDAGAI AGLNVVRIIN EPTGAAIAYG LDKKGGESNI LVYDLGGGTF 250
    DVSILTIDNG VFEVLSTSGD THLGGEDFDH RVMDYFIKLV KKKYNKDISK 300
    DHKALGKLRR ECELAKRSLS NQHQVRVEIE SLFDGVDFSE PLTRARFEEL 350
    NMDLFKKTME PVKKALKDAG LKKSDIDEIV LVGGSTRIPK VQQMLKDFFD 400
    GKEPSKGTNP DEAVAYGAAV QGGVLSGEGG EETQNILLLD VAPLSLGIET 450
    VGGVMTNIIP RNTVIPTKKS QVFTTYQDQQ TTVTINVYEG ERSMTKDNRE 500
    LGKFDLTGIL PAPRGVPQIE VTFEVDANGI LQVKAEDKVA KTSQSITITN 550
    DKGRLTEEEI EEMIREAEEF AEEDKIMKEK IDARNKLETY VYNMKSTVAD 600
    KEKLAKKISD EDKEKMEGVL KEALEWLEEN VNAEKEDYDE KLKEVELVCD 650
    PVIKSVYEKT EGENEDDDGD DHDEL 675
    Length:675
    Mass (Da):75,149
    Last modified:March 1, 2003 - v1
    Checksum:i6FF9FAE4B2A4702D
    GO
    Isoform 2 (identifier: Q8H1B3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         25-34: Missing.

    Note: Constructed according to the conserved gene model. No experimental confirmation available.

    Show »
    Length:665
    Mass (Da):74,075
    Checksum:iA36094C0C933358E
    GO

    Sequence cautioni

    The sequence AAB70400.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei25 – 3410Missing in isoform 2. CuratedVSP_042244

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY156728 mRNA. Translation: AAN60163.1.
    AC000106 Genomic DNA. Translation: AAB70400.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28392.1.
    CP002684 Genomic DNA. Translation: AEE28393.1.
    PIRiH86222.
    RefSeqiNP_001184944.1. NM_001198015.1. [Q8H1B3-2]
    NP_172382.4. NM_100779.5. [Q8H1B3-1]
    UniGeneiAt.42242.

    Genome annotation databases

    EnsemblPlantsiAT1G09080.1; AT1G09080.1; AT1G09080. [Q8H1B3-1]
    GeneIDi837429.
    KEGGiath:AT1G09080.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY156728 mRNA. Translation: AAN60163.1 .
    AC000106 Genomic DNA. Translation: AAB70400.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28392.1 .
    CP002684 Genomic DNA. Translation: AEE28393.1 .
    PIRi H86222.
    RefSeqi NP_001184944.1. NM_001198015.1. [Q8H1B3-2 ]
    NP_172382.4. NM_100779.5. [Q8H1B3-1 ]
    UniGenei At.42242.

    3D structure databases

    ProteinModelPortali Q8H1B3.
    SMRi Q8H1B3. Positions 49-654.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8H1B3. 2 interactions.
    MINTi MINT-7712705.
    STRINGi 3702.AT1G09080.1-P.

    Proteomic databases

    PRIDEi Q8H1B3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G09080.1 ; AT1G09080.1 ; AT1G09080 . [Q8H1B3-1 ]
    GeneIDi 837429.
    KEGGi ath:AT1G09080.

    Organism-specific databases

    TAIRi AT1G09080.

    Phylogenomic databases

    eggNOGi COG0443.
    HOGENOMi HOG000228135.
    InParanoidi Q8H1B3.
    KOi K09490.
    OMAi RDIKFLP.
    PhylomeDBi Q8H1B3.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1557-MONOMER.
    ARA:GQT-820-MONOMER.
    Reactomei REACT_108190. ATF6-alpha activates chaperone genes.

    Gene expression databases

    Genevestigatori Q8H1B3.

    Family and domain databases

    Gene3Di 1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProi IPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view ]
    Pfami PF00012. HSP70. 1 hit.
    [Graphical view ]
    PRINTSi PR00301. HEATSHOCK70.
    SUPFAMi SSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of an evolutionarily distinct novel BiP gene during the unfolded protein response in Arabidopsis thaliana."
      Noh S.J., Kwon C.S., Oh D.H., Moon J.S., Chung W.I.
      Gene 311:81-91(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana."
      Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M.
      Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family."
      Sung D.Y., Vierling E., Guy C.L.
      Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNAK GENE SUBFAMILY.
    6. "Heat induces the splicing by IRE1 of a mRNA encoding a transcription factor involved in the unfolded protein response in Arabidopsis."
      Deng Y., Humbert S., Liu J.X., Srivastava R., Rothstein S.J., Howell S.H.
      Proc. Natl. Acad. Sci. U.S.A. 108:7247-7252(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY DTT.
    7. "The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress."
      Mathur S., Vyas S., Kapoor S., Tyagi A.K.
      Plant Physiol. 157:1609-1627(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, SUBUNIT, NOMENCLATURE.

    Entry informationi

    Entry nameiMD37B_ARATH
    AccessioniPrimary (citable) accession number: Q8H1B3
    Secondary accession number(s): F4HZD5, O04022
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3