Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable mediator of RNA polymerase II transcription subunit 37b

Gene

MED37B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.By similarity
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mediator of RNA polymerase II transcription subunit 37b
Alternative name(s):
BiP chaperone BIP-L
Heat shock 70 kDa protein 13
Heat shock protein 70-13
Short name:
AtHsp70-13
Hsp70 protein BiP chaperone BIP-L
Luminal-binding protein 3
Short name:
AtBP3
Short name:
BiP3
Gene namesi
Name:MED37B
Synonyms:BIP-L, BIP3, HSP70-13, MED37_1
Ordered Locus Names:At1g09080
ORF Names:F7G19.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G09080.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation
  • Nucleus Curated

GO - Cellular componenti

  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • mediator complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000041543036 – 675Probable mediator of RNA polymerase II transcription subunit 37bAdd BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki496Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ8H1B3.
PRIDEiQ8H1B3.
ProMEXiQ8H1B3.

Expressioni

Inductioni

By tunicamycin and by DTT.2 Publications

Gene expression databases

GenevisibleiQ8H1B3. AT.

Interactioni

Subunit structurei

Component of the Mediator complex (PubMed:22021418). Interacts with BZIP28 (via C-terminus). MED37A/BIP1 and MED37B/BIP3 dissociates from BZIP28 under ER stress (PubMed:23624714).1 Publication1 Publication

Protein-protein interaction databases

BioGridi22670. 2 interactors.
IntActiQ8H1B3. 2 interactors.
MINTiMINT-7712705.
STRINGi3702.AT1G09080.1.

Structurei

3D structure databases

ProteinModelPortaliQ8H1B3.
SMRiQ8H1B3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi673 – 675Prevents secretion from ERPROSITE-ProRule annotation3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi557 – 665Glu-richAdd BLAST109

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0100. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiQ8H1B3.
KOiK09490.
OMAiYNMRSTI.
OrthoDBiEOG093604JO.
PhylomeDBiQ8H1B3.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8H1B3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIFIKENTAK MTRNKAIACL VFLTVLDFLM NIGAALMSSL AIEGEEQKLG
60 70 80 90 100
TVIGIDLGTT YSCVGVYHNK HVEIIANDQG NRITPSWVAF TDTERLIGEA
110 120 130 140 150
AKNQAAKNPE RTIFDPKRLI GRKFDDPDVQ RDIKFLPYKV VNKDGKPYIQ
160 170 180 190 200
VKVKGEEKLF SPEEISAMIL TKMKETAEAF LGKKIKDAVI TVPAYFNDAQ
210 220 230 240 250
RQATKDAGAI AGLNVVRIIN EPTGAAIAYG LDKKGGESNI LVYDLGGGTF
260 270 280 290 300
DVSILTIDNG VFEVLSTSGD THLGGEDFDH RVMDYFIKLV KKKYNKDISK
310 320 330 340 350
DHKALGKLRR ECELAKRSLS NQHQVRVEIE SLFDGVDFSE PLTRARFEEL
360 370 380 390 400
NMDLFKKTME PVKKALKDAG LKKSDIDEIV LVGGSTRIPK VQQMLKDFFD
410 420 430 440 450
GKEPSKGTNP DEAVAYGAAV QGGVLSGEGG EETQNILLLD VAPLSLGIET
460 470 480 490 500
VGGVMTNIIP RNTVIPTKKS QVFTTYQDQQ TTVTINVYEG ERSMTKDNRE
510 520 530 540 550
LGKFDLTGIL PAPRGVPQIE VTFEVDANGI LQVKAEDKVA KTSQSITITN
560 570 580 590 600
DKGRLTEEEI EEMIREAEEF AEEDKIMKEK IDARNKLETY VYNMKSTVAD
610 620 630 640 650
KEKLAKKISD EDKEKMEGVL KEALEWLEEN VNAEKEDYDE KLKEVELVCD
660 670
PVIKSVYEKT EGENEDDDGD DHDEL
Length:675
Mass (Da):75,149
Last modified:March 1, 2003 - v1
Checksum:i6FF9FAE4B2A4702D
GO
Isoform 2 (identifier: Q8H1B3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     25-34: Missing.

Note: Constructed according to the conserved gene model. No experimental confirmation available.
Show »
Length:665
Mass (Da):74,075
Checksum:iA36094C0C933358E
GO

Sequence cautioni

The sequence AAB70400 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04224425 – 34Missing in isoform 2. Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY156728 mRNA. Translation: AAN60163.1.
AC000106 Genomic DNA. Translation: AAB70400.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28392.1.
CP002684 Genomic DNA. Translation: AEE28393.1.
PIRiH86222.
RefSeqiNP_001184944.1. NM_001198015.2. [Q8H1B3-2]
NP_172382.4. NM_100779.5. [Q8H1B3-1]
UniGeneiAt.42242.

Genome annotation databases

EnsemblPlantsiAT1G09080.1; AT1G09080.1; AT1G09080. [Q8H1B3-1]
GeneIDi837429.
GrameneiAT1G09080.1; AT1G09080.1; AT1G09080.
KEGGiath:AT1G09080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY156728 mRNA. Translation: AAN60163.1.
AC000106 Genomic DNA. Translation: AAB70400.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28392.1.
CP002684 Genomic DNA. Translation: AEE28393.1.
PIRiH86222.
RefSeqiNP_001184944.1. NM_001198015.2. [Q8H1B3-2]
NP_172382.4. NM_100779.5. [Q8H1B3-1]
UniGeneiAt.42242.

3D structure databases

ProteinModelPortaliQ8H1B3.
SMRiQ8H1B3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22670. 2 interactors.
IntActiQ8H1B3. 2 interactors.
MINTiMINT-7712705.
STRINGi3702.AT1G09080.1.

Proteomic databases

PaxDbiQ8H1B3.
PRIDEiQ8H1B3.
ProMEXiQ8H1B3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G09080.1; AT1G09080.1; AT1G09080. [Q8H1B3-1]
GeneIDi837429.
GrameneiAT1G09080.1; AT1G09080.1; AT1G09080.
KEGGiath:AT1G09080.

Organism-specific databases

TAIRiAT1G09080.

Phylogenomic databases

eggNOGiKOG0100. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiQ8H1B3.
KOiK09490.
OMAiYNMRSTI.
OrthoDBiEOG093604JO.
PhylomeDBiQ8H1B3.

Miscellaneous databases

PROiQ8H1B3.

Gene expression databases

GenevisibleiQ8H1B3. AT.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMD37B_ARATH
AccessioniPrimary (citable) accession number: Q8H1B3
Secondary accession number(s): F4HZD5, O04022
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.