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Protein

Probable polyamine oxidase 4

Gene

PAO4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Flavoenzyme that catalyzes the oxidation of the secondary amino group of spermine. No activity detected when spermidine, putrescine or N1-acetylspermine are used as substrates.

Catalytic activityi

Spermine + O2 + H2O = spermidine + 3-aminopropanal + H2O2.1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Kineticsi

  1. KM=0.23 mM for spermine1 Publication

    pH dependencei

    Optimum pH is 7.5-8.5.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • polyamine catabolic process Source: TAIR

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT1G65840-MONOMER
    MetaCyc:AT1G65840-MONOMER
    BRENDAi1.5.3.16 399

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable polyamine oxidase 4 (EC:1.5.3.16)
    Short name:
    AtPAO4
    Alternative name(s):
    Amine oxidase 2
    Gene namesi
    Name:PAO4
    Ordered Locus Names:At1g65840
    ORF Names:F1E22.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G65840
    TAIRilocus:2018571 AT1G65840

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Peroxisome

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003525101 – 497Probable polyamine oxidase 4Add BLAST497

    Proteomic databases

    PaxDbiQ8H191

    Expressioni

    Tissue specificityi

    Highly expressed in roots, flowers and greening cotelydons. Lower expression in other tissues.1 Publication

    Inductioni

    By abscisic acid, salicylic acid, wounding and flagellin 22, a pathogen elicitor.1 Publication

    Gene expression databases

    ExpressionAtlasiQ8H191 baseline and differential
    GenevisibleiQ8H191 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G65840.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H191
    SMRiQ8H191
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family.Curated

    Phylogenomic databases

    eggNOGiKOG0029 Eukaryota
    ENOG410XSNC LUCA
    HOGENOMiHOG000037651
    InParanoidiQ8H191
    KOiK17839
    OMAiHAGPINP
    OrthoDBiEOG093607L1
    PhylomeDBiQ8H191

    Family and domain databases

    Gene3Di3.50.50.60, 3 hits
    InterProiView protein in InterPro
    IPR002937 Amino_oxidase
    IPR036188 FAD/NAD-bd_sf
    IPR001613 Flavin_amine_oxidase
    PfamiView protein in Pfam
    PF01593 Amino_oxidase, 1 hit
    PRINTSiPR00757 AMINEOXDASEF
    SUPFAMiSSF51905 SSF51905, 2 hits

    Sequencei

    Sequence statusi: Complete.

    Q8H191-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDKKKNSFPD NLPEGTISEL MQKQNNVQPS VIVIGSGISG LAAARNLSEA
    60 70 80 90 100
    SFKVTVLESR DRIGGRIHTD YSFGCPVDMG ASWLHGVSDE NPLAPIIRRL
    110 120 130 140 150
    GLTLYRTSGD DSILYDHDLE SYGLFDMHGN KIPPQLVTKV GDAFKRILEE
    160 170 180 190 200
    TEKIRDETAN DMSVLQGISI VLDRNPELRQ EGMAYEVLQW YLCRMEAWFA
    210 220 230 240 250
    VDANLISLKC WDQDECLSGG HGLMVQGYEP VIRTIAKDLD IRLNHRVTKV
    260 270 280 290 300
    VRTSNNKVIV AVEGGTNFVA DAVIITVPIG VLKANLIQFE PELPQWKTSA
    310 320 330 340 350
    ISGLGVGNEN KIALRFDRAF WPNVEFLGMV APTSYACGYF LNLHKATGHP
    360 370 380 390 400
    VLVYMAAGNL AQDLEKLSDE ATANFVMLQL KKMFPDAPDP AQYLVTRWGT
    410 420 430 440 450
    DPNTLGCYAY DVVGMPEDLY PRLGEPVDNI FFGGEAVNVE HQGSAHGAFL
    460 470 480 490
    AGVSASQNCQ RYIFERLGAW EKLKLVSLMG NSDILETATV PLQISRM
    Length:497
    Mass (Da):54,931
    Last modified:March 1, 2003 - v1
    Checksum:i69CEE30B841B022C
    GO

    Sequence cautioni

    The sequence AAF23834 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti283K → R in AAK43885 (PubMed:14593172).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF364953 mRNA Translation: AAO85405.1
    AC007234 Genomic DNA Translation: AAF23834.1 Sequence problems.
    CP002684 Genomic DNA Translation: AEE34430.1
    AF370508 mRNA Translation: AAK43885.1
    BT000353 mRNA Translation: AAN15672.1
    PIRiD96682
    RefSeqiNP_176759.1, NM_105256.4
    UniGeneiAt.16379

    Genome annotation databases

    EnsemblPlantsiAT1G65840.1; AT1G65840.1; AT1G65840
    GeneIDi842894
    GrameneiAT1G65840.1; AT1G65840.1; AT1G65840
    KEGGiath:AT1G65840

    Similar proteinsi

    Entry informationi

    Entry nameiPAO4_ARATH
    AccessioniPrimary (citable) accession number: Q8H191
    Secondary accession number(s): Q94JZ7, Q9SHX4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
    Last sequence update: March 1, 2003
    Last modified: April 25, 2018
    This is version 104 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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