Reviewed, UniProtKB/Swiss-Prot Q8H166 (ALEU_ARATH)

Names and origin

Protein names

Recommended name:
    Thiol protease aleurain
      Short name=AtALEU
    EC=3.4.22.16
Alternative name(s):
    Senescence-associated gene product 2

Gene names

Name:	ALEU
Synonyms:	AALP, SAG2
Ordered Locus Names:	At5g60360
ORF Names:	MUF9.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation.
Catalytic activity	Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.
Subunit structure	Interacts with VSR1/BP80B. Ref.1
Subcellular location	Vacuole. Note: Predominantly vacuolar. From the Golgi apparatus, transported to the lytic vacuole (LV) in clathrin-coated vesicles (CCVs) via the prevacuolar compartment (PVC). In root elongating cells and stomata, localized in central vacuole and in small compartments (LVs, PVCs, Golgi bodies or small vacuoles). Limited to central vacuole in root elongated cells, leaf epidermal cells and trichomes of the lower face of the leaf blade. Limited to small compartments in root cap and apex cells, hypocotyl parenchyma cells and trichomes of the upper face of the leaf blade. Ref.1 Ref.9
Tissue specificity	Expressed in leaves (at protein level). Ref.6 Ref.7
Induction	Induced during senescence. Strongly induced by ethylene and slightly by abscisic acid. Repressed by cytokinin and darkness. Seems to be not affected by dehydration. Ref.6 Ref.8 Ref.10
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Vacuole
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aging Ref.6 Inferred from expression pattern. Source: TAIR proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	vacuole Inferred from direct assay. Source: TAIR
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449450,EBI-449157

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8H166-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 140	119	Activation peptide By similarity		PRO_0000026414
	Chain	141 – 358	218	Thiol protease aleurain		PRO_0000026415
Regions
	Region	22 – 42	21	Interaction with VSR1
Sites
	Active site	165	1	By similarity
	Active site	305	1	By similarity
	Active site	325	1	By similarity
Amino acid modifications
	Glycosylation	125	1	N-linked (GlcNAc...) Potential
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Disulfide bond	162 ↔ 205		By similarity
	Disulfide bond	196 ↔ 238		By similarity
	Disulfide bond	296 ↔ 346		By similarity
Experimental info
	Sequence conflict	16	1	V → F in AAN31820. Ref.4
	Sequence conflict	166	1	W → V Ref.6
	Sequence conflict	205	1	C → S Ref.6
	Sequence conflict	230	1	P → R Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 3B610AB85F81C31D	FASTA	358	38,959
	10 20 30 40 50 60 MSAKTILSSV VLVVLVAASA AANIGFDESN PIRMVSDGLR EVEESVSQIL GQSRHVLSFA 70 80 90 100 110 120 RFTHRYGKKY QNVEEMKLRF SIFKENLDLI RSTNKKGLSY KLGVNQFADL TWQEFQRTKL 130 140 150 160 170 180 GAAQNCSATL KGSHKVTEAA LPETKDWRED GIVSPVKDQG GCGSCWTFST TGALEAAYHQ 190 200 210 220 230 240 AFGKGISLSE QQLVDCAGAF NNYGCNGGLP SQAFEYIKSN GGLDTEKAYP YTGKDETCKF 250 260 270 280 290 300 SAENVGVQVL NSVNITLGAE DELKHAVGLV RPVSIAFEVI HSFRLYKSGV YTDSHCGSTP 310 320 330 340 350 MDVNHAVLAV GYGVEDGVPY WLIKNSWGAD WGDKGYFKME MGKNMCGIAT CASYPVVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The plant vacuolar sorting receptor AtELP is involved in transport of NH(2)-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana." Ahmed S.U., Rojo E., Kovaleva V., Venkataraman S., Dombrowski J.E., Matsuoka K., Raikhel N.V. J. Cell Biol. 149:1335-1344(2000) [PubMed: 10871276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH VSR1.
[2]	"Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells." Stracke R., Palme K. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed: 9679202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in Arabidopsis." Hensel L.L., Grbic V., Baumgarten D.A., Bleecker A.B. Plant Cell 5:553-564(1993) [PubMed: 8518555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 137-231, INDUCTION, TISSUE SPECIFICITY. Strain: cv. Landsberg erecta. Tissue: Leaf.
[7]	"Monoclonal antibodies to barley aleurain and homologs from other plants." Rogers S.W., Burks M., Rogers J.C. Plant J. 11:1359-1368(1997) [PubMed: 9225474] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"A comparison of the expression patterns of several senescence-associated genes in response to stress and hormone treatment." Weaver L.M., Gan S., Quirino B., Amasino R.M. Plant Mol. Biol. 37:455-469(1998) [PubMed: 9617813] [Abstract] Cited for: INDUCTION.
[9]	"Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins." Flueckiger R., De Caroli M., Piro G., Dalessandro G., Neuhaus J.-M., Di Sansebastiano G.-P. J. Exp. Bot. 54:1577-1584(2003) [PubMed: 12730271] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"Large-scale identification of leaf senescence-associated genes." Gepstein S., Sabehi G., Carp M.-J., Hajouj T., Nesher M.F.O., Yariv I., Dor C., Bassani M. Plant J. 36:629-642(2003) [PubMed: 14617064] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF233883 mRNA. Translation: AAF43041.1. AF083703 mRNA. Translation: AAN60262.1. AB011483 Genomic DNA. Translation: BAB08221.1. AF360273 mRNA. Translation: AAK25983.1. BT000673 mRNA. Translation: AAN31819.1. BT000674 mRNA. Translation: AAN31820.1. BT000676 mRNA. Translation: AAN31822.1. AY088662 mRNA. Translation: AAM66984.1.
IPI	IPI00537494.
PIR	PQ0650.
RefSeq	NP_568921.1.
UniGene	At.25414
3D structure databases
HSSP	HSSP built from PDB template 1CJL based on UniProtKB P07711.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8H166. 1 interaction.
Protein family/group databases
MEROPS	C01.A08.
Proteomic databases
PRIDE	Q8H166.
Genome annotation databases
GeneID	836158.
GenomeReviews	Gene locus AT5G60360 in contig BA000015_GR.
NMPDR	fig|3702.1.peg.28043.
Organism-specific databases
GeneFarm	5031. 478.
TAIR	At5g60360.
Phylogenomic databases
OMA	Q8H166. CASYPVV.
Enzyme and pathway databases
BRENDA	3.4.22.16. 302.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALEU_ARATH

Accession

Primary (citable) accession number: Q8H166
Secondary accession number(s): Q9LL83, Q9S9A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)