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Protein

Thiol protease aleurain

Gene

ALEU

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation.

Catalytic activityi

Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei165 – 1651By similarity
Active sitei305 – 3051By similarity
Active sitei325 – 3251By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciARA:AT5G60360-MONOMER.
ARA:GQT-2510-MONOMER.
ARA:GQT-2511-MONOMER.

Protein family/group databases

MEROPSiI29.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol protease aleurain (EC:3.4.22.16)
Short name:
AtALEU
Alternative name(s):
Senescence-associated gene product 2
Gene namesi
Name:ALEU
Synonyms:AALP, SAG2
Ordered Locus Names:At5g60360
ORF Names:MUF9.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G60360.

Subcellular locationi

  • Vacuole 2 Publications

  • Note: Predominantly vacuolar. From the Golgi apparatus, transported to the lytic vacuole (LV) in clathrin-coated vesicles (CCVs) via the prevacuolar compartment (PVC). In root elongating cells and stomata, localized in central vacuole and in small compartments (LVs, PVCs, Golgi bodies or small vacuoles). Limited to central vacuole in root elongated cells, leaf epidermal cells and trichomes of the lower face of the leaf blade. Limited to small compartments in root cap and apex cells, hypocotyl parenchyma cells and trichomes of the upper face of the leaf blade.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 140119Activation peptideBy similarityPRO_0000026414Add
BLAST
Chaini141 – 358218Thiol protease aleurainPRO_0000026415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence analysis
Disulfide bondi162 ↔ 205By similarity
Disulfide bondi196 ↔ 238By similarity
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence analysis
Disulfide bondi296 ↔ 346By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ8H166.
PRIDEiQ8H166.

Expressioni

Tissue specificityi

Expressed in leaves (at protein level).2 Publications

Inductioni

Induced during senescence. Strongly induced by ethylene and slightly by abscisic acid. Repressed by cytokinin and darkness. Seems to be not affected by dehydration.3 Publications

Gene expression databases

ExpressionAtlasiQ8H166. baseline and differential.
GenevisibleiQ8H166. AT.

Interactioni

Subunit structurei

Interacts with VSR1/BP80B.1 Publication

Protein-protein interaction databases

BioGridi21402. 2 interactions.
IntActiQ8H166. 1 interaction.
STRINGi3702.AT5G60360.3.

Structurei

3D structure databases

ProteinModelPortaliQ8H166.
SMRiQ8H166. Positions 66-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 4221Interaction with VSR1Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230774.
InParanoidiQ8H166.
KOiK01366.
PhylomeDBiQ8H166.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8H166-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAKTILSSV VLVVLVAASA AANIGFDESN PIRMVSDGLR EVEESVSQIL
60 70 80 90 100
GQSRHVLSFA RFTHRYGKKY QNVEEMKLRF SIFKENLDLI RSTNKKGLSY
110 120 130 140 150
KLGVNQFADL TWQEFQRTKL GAAQNCSATL KGSHKVTEAA LPETKDWRED
160 170 180 190 200
GIVSPVKDQG GCGSCWTFST TGALEAAYHQ AFGKGISLSE QQLVDCAGAF
210 220 230 240 250
NNYGCNGGLP SQAFEYIKSN GGLDTEKAYP YTGKDETCKF SAENVGVQVL
260 270 280 290 300
NSVNITLGAE DELKHAVGLV RPVSIAFEVI HSFRLYKSGV YTDSHCGSTP
310 320 330 340 350
MDVNHAVLAV GYGVEDGVPY WLIKNSWGAD WGDKGYFKME MGKNMCGIAT

CASYPVVA
Length:358
Mass (Da):38,959
Last modified:July 19, 2005 - v2
Checksum:i3B610AB85F81C31D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161V → F in AAN31820 (PubMed:14593172).Curated
Sequence conflicti166 – 1661W → V (PubMed:8518555).Curated
Sequence conflicti205 – 2051C → S (PubMed:8518555).Curated
Sequence conflicti230 – 2301P → R (PubMed:8518555).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233883 mRNA. Translation: AAF43041.1.
AF083703 mRNA. Translation: AAN60262.1.
AB011483 Genomic DNA. Translation: BAB08221.1.
CP002688 Genomic DNA. Translation: AED97313.1.
AF360273 mRNA. Translation: AAK25983.1.
BT000673 mRNA. Translation: AAN31819.1.
BT000674 mRNA. Translation: AAN31820.1.
BT000676 mRNA. Translation: AAN31822.1.
AY088662 mRNA. Translation: AAM66984.1.
PIRiPQ0650.
RefSeqiNP_568921.1. NM_125429.3. [Q8H166-1]
UniGeneiAt.25414.

Genome annotation databases

EnsemblPlantsiAT5G60360.1; AT5G60360.1; AT5G60360. [Q8H166-1]
GeneIDi836158.
KEGGiath:AT5G60360.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233883 mRNA. Translation: AAF43041.1.
AF083703 mRNA. Translation: AAN60262.1.
AB011483 Genomic DNA. Translation: BAB08221.1.
CP002688 Genomic DNA. Translation: AED97313.1.
AF360273 mRNA. Translation: AAK25983.1.
BT000673 mRNA. Translation: AAN31819.1.
BT000674 mRNA. Translation: AAN31820.1.
BT000676 mRNA. Translation: AAN31822.1.
AY088662 mRNA. Translation: AAM66984.1.
PIRiPQ0650.
RefSeqiNP_568921.1. NM_125429.3. [Q8H166-1]
UniGeneiAt.25414.

3D structure databases

ProteinModelPortaliQ8H166.
SMRiQ8H166. Positions 66-358.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21402. 2 interactions.
IntActiQ8H166. 1 interaction.
STRINGi3702.AT5G60360.3.

Protein family/group databases

MEROPSiI29.003.

Proteomic databases

PaxDbiQ8H166.
PRIDEiQ8H166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G60360.1; AT5G60360.1; AT5G60360. [Q8H166-1]
GeneIDi836158.
KEGGiath:AT5G60360.

Organism-specific databases

TAIRiAT5G60360.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230774.
InParanoidiQ8H166.
KOiK01366.
PhylomeDBiQ8H166.

Enzyme and pathway databases

BioCyciARA:AT5G60360-MONOMER.
ARA:GQT-2510-MONOMER.
ARA:GQT-2511-MONOMER.

Miscellaneous databases

PROiQ8H166.

Gene expression databases

ExpressionAtlasiQ8H166. baseline and differential.
GenevisibleiQ8H166. AT.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The plant vacuolar sorting receptor AtELP is involved in transport of NH(2)-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana."
    Ahmed S.U., Rojo E., Kovaleva V., Venkataraman S., Dombrowski J.E., Matsuoka K., Raikhel N.V.
    J. Cell Biol. 149:1335-1344(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH VSR1.
  2. "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells."
    Stracke R., Palme K.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in Arabidopsis."
    Hensel L.L., Grbic V., Baumgarten D.A., Bleecker A.B.
    Plant Cell 5:553-564(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 137-231, INDUCTION, TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
    Tissue: Leaf.
  8. "Monoclonal antibodies to barley aleurain and homologs from other plants."
    Rogers S.W., Burks M., Rogers J.C.
    Plant J. 11:1359-1368(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. "A comparison of the expression patterns of several senescence-associated genes in response to stress and hormone treatment."
    Weaver L.M., Gan S., Quirino B., Amasino R.M.
    Plant Mol. Biol. 37:455-469(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins."
    Flueckiger R., De Caroli M., Piro G., Dalessandro G., Neuhaus J.-M., Di Sansebastiano G.-P.
    J. Exp. Bot. 54:1577-1584(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  11. "Large-scale identification of leaf senescence-associated genes."
    Gepstein S., Sabehi G., Carp M.-J., Hajouj T., Nesher M.F.O., Yariv I., Dor C., Bassani M.
    Plant J. 36:629-642(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiALEU_ARATH
AccessioniPrimary (citable) accession number: Q8H166
Secondary accession number(s): Q9LL83, Q9S9A9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: July 6, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.