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Protein

Protein NRT1/ PTR FAMILY 4.6

Gene

NPF4.6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity proton-dependent nitrate transporter. Involved in constitutive nitrate uptake. Not involved in histidine or dipeptides transport. Involved in (+)-abscisic acid (ABA) transport, but not in gibberellin, indole-3-acetic acid or jasmonic acid import. Mediates cellular ABA uptake. Nitrate does not compete with abscisic acid as a substrate of NPF4.6 (PubMed:24084651).3 Publications

Kineticsi

  1. KM=5.9 mM for nitrate2 Publications
  2. KM=5 µM for (+)-abscisic acid (in insect cells)2 Publications

    GO - Molecular functioni

    • abscisic acid transporter activity Source: TAIR
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • abscisic acid transport Source: TAIR
    • nitrate assimilation Source: UniProtKB-KW
    • regulation of stomatal movement Source: TAIR
    • response to nematode Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Nitrate assimilation, Symport, Transport

    Protein family/group databases

    TCDBi2.A.17.3.16. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 4.6
    Short name:
    AtNPF4.6
    Alternative name(s):
    Nitrate transporter 1.2
    Nitrate transporter NTL1
    Protein ABA-IMPORTING TRANSPORTER 1
    Gene namesi
    Name:NPF4.6
    Synonyms:AIT1, NRT1.2, NTL1, NTR1:2
    Ordered Locus Names:At1g69850
    ORF Names:T17F3.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G69850.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei28 – 4821HelicalSequence analysisAdd
    BLAST
    Transmembranei75 – 9521HelicalSequence analysisAdd
    BLAST
    Transmembranei96 – 11621HelicalSequence analysisAdd
    BLAST
    Transmembranei142 – 16221HelicalSequence analysisAdd
    BLAST
    Transmembranei184 – 20421HelicalSequence analysisAdd
    BLAST
    Transmembranei211 – 23121HelicalSequence analysisAdd
    BLAST
    Transmembranei343 – 36321HelicalSequence analysisAdd
    BLAST
    Transmembranei391 – 41121HelicalSequence analysisAdd
    BLAST
    Transmembranei428 – 44821HelicalSequence analysisAdd
    BLAST
    Transmembranei465 – 48521HelicalSequence analysisAdd
    BLAST
    Transmembranei508 – 52821HelicalSequence analysisAdd
    BLAST
    Transmembranei554 – 57421HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Reduced effect of ABA on seed germination and postgermination growth and increased number of open stomata.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 585585Protein NRT1/ PTR FAMILY 4.6PRO_0000399953Add
    BLAST

    Proteomic databases

    PaxDbiQ8H157.
    PRIDEiQ8H157.

    Expressioni

    Tissue specificityi

    Expressed in root hairs and in epidermis of both root tips and mature regions of roots. Detected in shoots, stems, flowers, siliques and imbibed seeds. Expressed in vascular tissues in cotyledons, trus leaves, hypocotyls, roots and inflorescence stems.3 Publications

    Inductioni

    Not regulated by nitrate. Down-regulated upon nematode infection.3 Publications

    Gene expression databases

    GenevisibleiQ8H157. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi28542. 21 interactions.
    IntActiQ8H157. 2 interactions.
    STRINGi3702.AT1G69850.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H157.
    SMRiQ8H157. Positions 15-579.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237400.
    InParanoidiQ8H157.
    OMAiCKPGDIC.
    PhylomeDBiQ8H157.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 2 hits.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q8H157-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEVEEEVSRW EGYADWRNRA AVKGRHGGML AASFVLVVEI LENLAYLANA
    60 70 80 90 100
    SNLVLYLREY MHMSPSKSAN DVTNFMGTAF LLALLGGFLS DAFFSTFQIF
    110 120 130 140 150
    LISASIEFLG LIILTIQART PSLMPPSCDS PTCEEVSGSK AAMLFVGLYL
    160 170 180 190 200
    VALGVGGIKG SLASHGAEQF DESTPKGRKQ RSTFFNYFVF CLACGALVAV
    210 220 230 240 250
    TFVVWLEDNK GWEWGFGVST IAIFVSILIF LSGSRFYRNK IPCGSPLTTI
    260 270 280 290 300
    LKVLLAASVK CCSSGSSSNA VASMSVSPSN HCVSKGKKEV ESQGELEKPR
    310 320 330 340 350
    QEEALPPRAQ LTNSLKVLNG AADEKPVHRL LECTVQQVED VKIVLKMLPI
    360 370 380 390 400
    FACTIMLNCC LAQLSTFSVQ QAASMNTKIG SLKIPPASLP IFPVVFIMIL
    410 420 430 440 450
    APIYDHLIIP FARKATKTET GVTHLQRIGV GLVLSILAMA VAALVEIKRK
    460 470 480 490 500
    GVAKDSGLLD SKETLPVTFL WIALQYLFLG SADLFTLAGL LEYFFTEAPS
    510 520 530 540 550
    SMRSLATSLS WASLAMGYYL SSVIVSIVNS ITGSSGNTPW LRGKSINRYK
    560 570 580
    LDYFYWLMCV LSAANFLHYL FWAMRYKYRS TGSRS
    Length:585
    Mass (Da):63,982
    Last modified:March 1, 2003 - v1
    Checksum:i8AC024843574D3CC
    GO

    Sequence cautioni

    The sequence AAG52554.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti29 – 291M → V in AAL32531 (PubMed:14593172).Curated
    Sequence conflicti29 – 291M → V in AAO00921 (PubMed:14593172).Curated
    Sequence conflicti392 – 3921F → L in AAC28086 (PubMed:10449574).Curated
    Sequence conflicti473 – 4731A → G in AAC28086 (PubMed:10449574).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF073361 mRNA. Translation: AAC28086.1.
    AC010675 Genomic DNA. Translation: AAG52554.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34991.1.
    BT000720 mRNA. Translation: AAN31862.1.
    AY062453 mRNA. Translation: AAL32531.1.
    BT002561 mRNA. Translation: AAO00921.1.
    PIRiT51361.
    RefSeqiNP_564978.1. NM_105653.4.
    UniGeneiAt.21144.

    Genome annotation databases

    EnsemblPlantsiAT1G69850.1; AT1G69850.1; AT1G69850.
    GeneIDi843321.
    GrameneiAT1G69850.1; AT1G69850.1; AT1G69850.
    KEGGiath:AT1G69850.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF073361 mRNA. Translation: AAC28086.1.
    AC010675 Genomic DNA. Translation: AAG52554.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34991.1.
    BT000720 mRNA. Translation: AAN31862.1.
    AY062453 mRNA. Translation: AAL32531.1.
    BT002561 mRNA. Translation: AAO00921.1.
    PIRiT51361.
    RefSeqiNP_564978.1. NM_105653.4.
    UniGeneiAt.21144.

    3D structure databases

    ProteinModelPortaliQ8H157.
    SMRiQ8H157. Positions 15-579.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi28542. 21 interactions.
    IntActiQ8H157. 2 interactions.
    STRINGi3702.AT1G69850.1.

    Protein family/group databases

    TCDBi2.A.17.3.16. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Proteomic databases

    PaxDbiQ8H157.
    PRIDEiQ8H157.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G69850.1; AT1G69850.1; AT1G69850.
    GeneIDi843321.
    GrameneiAT1G69850.1; AT1G69850.1; AT1G69850.
    KEGGiath:AT1G69850.

    Organism-specific databases

    TAIRiAT1G69850.

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237400.
    InParanoidiQ8H157.
    OMAiCKPGDIC.
    PhylomeDBiQ8H157.

    Miscellaneous databases

    PROiQ8H157.

    Gene expression databases

    GenevisibleiQ8H157. AT.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 2 hits.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning and functional characterization of an Arabidopsis nitrate transporter gene that encodes a constitutive component of low-affinity uptake."
      Huang N.C., Liu K.H., Lo H.J., Tsay Y.F.
      Plant Cell 11:1381-1392(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION BY NITRATE, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana: responses to nitrate provision."
      Okamoto M., Vidmar J.J., Glass A.D.
      Plant Cell Physiol. 44:304-317(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY NITRATE.
    6. "Nematode-induced changes of transporter gene expression in Arabidopsis roots."
      Hammes U.Z., Schachtman D.P., Berg R.H., Nielsen E., Koch W., McIntyre L.M., Taylor C.G.
      Mol. Plant Microbe Interact. 18:1247-1257(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY NEMATODES.
    7. "Nitrate transporters and peptide transporters."
      Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.
      FEBS Lett. 581:2290-2300(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, GENE FAMILY.
    8. "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance."
      Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z., Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W., Gong J.M.
      Plant Cell 22:1633-1646(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    9. "Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor."
      Kanno Y., Hanada A., Chiba Y., Ichikawa T., Nakazawa M., Matsui M., Koshiba T., Kamiya Y., Seo M.
      Proc. Natl. Acad. Sci. U.S.A. 109:9653-9658(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    10. "Nitrate does not compete with abscisic acid as a substrate of AtNPF4.6/NRT1.2/AIT1 in Arabidopsis."
      Kanno Y., Kamiya Y., Seo M.
      Plant Signal. Behav. 8:0-0(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPTR19_ARATH
    AccessioniPrimary (citable) accession number: Q8H157
    Secondary accession number(s): O81393, Q8W4N9, Q9CAR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: March 1, 2003
    Last modified: February 17, 2016
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.