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Protein

Purple acid phosphatase 3

Gene

PAP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi81IronBy similarity1
Metal bindingi114IronBy similarity1
Metal bindingi114ZincBy similarity1
Metal bindingi117IronBy similarity1
Metal bindingi152ZincBy similarity1
Metal bindingi246ZincBy similarity1
Active sitei255Proton donorBy similarity1
Metal bindingi281ZincBy similarity1
Metal bindingi283IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G14700-MONOMER.
ReactomeiR-ATH-196843. Vitamin B2 (riboflavin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 3 (EC:3.1.3.2)
Gene namesi
Name:PAP3
Ordered Locus Names:At1g14700
ORF Names:F10B6.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G14700.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB-SubCell
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000037280833 – 366Purple acid phosphatase 3Add BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8H129.

PTM databases

iPTMnetiQ8H129.

Expressioni

Tissue specificityi

Expressed in stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevisibleiQ8H129. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi23275. 122 interactors.
STRINGi3702.AT1G14700.1.

Structurei

3D structure databases

ProteinModelPortaliQ8H129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni281 – 283Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG2679. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000239892.
KOiK01078.
OMAiRWVCMRS.
OrthoDBiEOG09360IST.
PhylomeDBiQ8H129.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8H129-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTYIYRDTKI TTKSTIPFLI FFLFCFSNLS MATLKHKPVN LVFYVYNLII
60 70 80 90 100
IFSSHSSTAE LRRLLQPSKT DGTVSFLVIG DWGRRGSYNQ SQVALQMGEI
110 120 130 140 150
GEKLDIDFVI STGDNFYDNG LTSLHDPLFQ DSFTNIYTAP SLQKPWYSVL
160 170 180 190 200
GNHDYRGDVR AQLSPMLRAL DNRWVCMRSF IVNAEIVDLF FVDTTPFVDK
210 220 230 240 250
YFIQPNKHVY DWSGVLPRQT YLNNLLKELD VALRESVAKW KIVIGHHTIK
260 270 280 290 300
SAGHHGNTIE LEKHLLPILQ ANEVDLYVNG HDHCLEHISS VDSNIQFMTS
310 320 330 340 350
GGGSKAWKGG DVNYVEPEEM RFYYDGQGFM SVHVSEAELR VVFYDVFGHV
360
LHHWKKTYKE ALYFAS
Length:366
Mass (Da):42,052
Last modified:March 1, 2003 - v1
Checksum:i427FD0C4B474DCC0
GO
Isoform 2 (identifier: Q8H129-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-150: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:364
Mass (Da):41,840
Checksum:i192AA2367701CEC0
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037188149 – 150Missing in isoform 2. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY686599 mRNA. Translation: AAT95435.1.
AC006917 Genomic DNA. Translation: AAF79221.1.
CP002684 Genomic DNA. Translation: AEE29210.1.
CP002684 Genomic DNA. Translation: AEE29211.1.
AY070051 mRNA. Translation: AAL49808.2.
BT000877 mRNA. Translation: AAN41277.1.
PIRiD86281.
RefSeqiNP_001077538.1. NM_001084069.1. [Q8H129-2]
NP_172923.3. NM_101339.5. [Q8H129-1]
UniGeneiAt.28419.

Genome annotation databases

EnsemblPlantsiAT1G14700.1; AT1G14700.1; AT1G14700. [Q8H129-1]
GeneIDi838035.
GrameneiAT1G14700.1; AT1G14700.1; AT1G14700.
KEGGiath:AT1G14700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY686599 mRNA. Translation: AAT95435.1.
AC006917 Genomic DNA. Translation: AAF79221.1.
CP002684 Genomic DNA. Translation: AEE29210.1.
CP002684 Genomic DNA. Translation: AEE29211.1.
AY070051 mRNA. Translation: AAL49808.2.
BT000877 mRNA. Translation: AAN41277.1.
PIRiD86281.
RefSeqiNP_001077538.1. NM_001084069.1. [Q8H129-2]
NP_172923.3. NM_101339.5. [Q8H129-1]
UniGeneiAt.28419.

3D structure databases

ProteinModelPortaliQ8H129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23275. 122 interactors.
STRINGi3702.AT1G14700.1.

PTM databases

iPTMnetiQ8H129.

Proteomic databases

PaxDbiQ8H129.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G14700.1; AT1G14700.1; AT1G14700. [Q8H129-1]
GeneIDi838035.
GrameneiAT1G14700.1; AT1G14700.1; AT1G14700.
KEGGiath:AT1G14700.

Organism-specific databases

TAIRiAT1G14700.

Phylogenomic databases

eggNOGiKOG2679. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000239892.
KOiK01078.
OMAiRWVCMRS.
OrthoDBiEOG09360IST.
PhylomeDBiQ8H129.

Enzyme and pathway databases

BioCyciARA:AT1G14700-MONOMER.
ReactomeiR-ATH-196843. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

PROiQ8H129.

Gene expression databases

GenevisibleiQ8H129. AT.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPA3_ARATH
AccessioniPrimary (citable) accession number: Q8H129
Secondary accession number(s): Q8VYS7, Q9LQW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.