Reviewed, UniProtKB/Swiss-Prot Q8H129 (PPA3_ARATH)

Names and origin

Protein names

Recommended name:
    Purple acid phosphatase 3
    EC=3.1.3.2

Gene names

Name:	PAP3
Ordered Locus Names:	At1g14700
ORF Names:	F10B6.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in stems, leaves, flowers and siliques. Ref.1
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from direct assay. Source: TAIR
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8H129-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8H129-2)

The sequence of this isoform differs from the canonical sequence as follows:
     149-150: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 366	334	Purple acid phosphatase 3		PRO_0000372808
Regions
	Region	281 – 283	3	Substrate binding By similarity
Sites
	Active site	255	1	Proton donor By similarity
	Metal binding	246	1	Zinc By similarity
	Metal binding	281	1	Zinc By similarity
	Metal binding	283	1	Iron By similarity
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	149 – 150	2	Missing in isoform 2.		VSP_037188

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 427FD0C4B474DCC0	FASTA	366	42,052
	10 20 30 40 50 60 MTYIYRDTKI TTKSTIPFLI FFLFCFSNLS MATLKHKPVN LVFYVYNLII IFSSHSSTAE 70 80 90 100 110 120 LRRLLQPSKT DGTVSFLVIG DWGRRGSYNQ SQVALQMGEI GEKLDIDFVI STGDNFYDNG 130 140 150 160 170 180 LTSLHDPLFQ DSFTNIYTAP SLQKPWYSVL GNHDYRGDVR AQLSPMLRAL DNRWVCMRSF 190 200 210 220 230 240 IVNAEIVDLF FVDTTPFVDK YFIQPNKHVY DWSGVLPRQT YLNNLLKELD VALRESVAKW 250 260 270 280 290 300 KIVIGHHTIK SAGHHGNTIE LEKHLLPILQ ANEVDLYVNG HDHCLEHISS VDSNIQFMTS 310 320 330 340 350 360 GGGSKAWKGG DVNYVEPEEM RFYYDGQGFM SVHVSEAELR VVFYDVFGHV LHHWKKTYKE ALYFAS
	Isoform 2. Checksum: 192AA2367701CEC0	FASTA	364	41,840
	10 20 30 40 50 60 MTYIYRDTKI TTKSTIPFLI FFLFCFSNLS MATLKHKPVN LVFYVYNLII IFSSHSSTAE 70 80 90 100 110 120 LRRLLQPSKT DGTVSFLVIG DWGRRGSYNQ SQVALQMGEI GEKLDIDFVI STGDNFYDNG 130 140 150 160 170 180 LTSLHDPLFQ DSFTNIYTAP SLQKPWYSGN HDYRGDVRAQ LSPMLRALDN RWVCMRSFIV 190 200 210 220 230 240 NAEIVDLFFV DTTPFVDKYF IQPNKHVYDW SGVLPRQTYL NNLLKELDVA LRESVAKWKI 250 260 270 280 290 300 VIGHHTIKSA GHHGNTIELE KHLLPILQAN EVDLYVNGHD HCLEHISSVD SNIQFMTSGG 310 320 330 340 350 360 GSKAWKGGDV NYVEPEEMRF YYDGQGFMSV HVSEAELRVV FYDVFGHVLH HWKKTYKEAL YFAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY686599 mRNA. Translation: AAT95435.1. AC006917 Genomic DNA. Translation: AAF79221.1. AY070051 mRNA. Translation: AAL49808.2. BT000877 mRNA. Translation: AAN41277.1.
IPI	IPI00522072. IPI00846554.
PIR	D86281.
RefSeq	NP_001077538.1. NP_172923.3.
UniGene	At.28419
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8H129.
Genome annotation databases
GeneID	838035.
GenomeReviews	Gene locus AT1G14700 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g14700.
Phylogenomic databases
OMA	TANAENE.
Gene expression databases
Genevestigator	Q8H129.
Family and domain databases
InterPro	IPR004843. M-pesterase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA3_ARATH

Accession

Primary (citable) accession number: Q8H129
Secondary accession number(s): Q8VYS7, Q9LQW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)