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Q8H129

- PPA3_ARATH

UniProt

Q8H129 - PPA3_ARATH

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Protein

Purple acid phosphatase 3

Gene
PAP3, At1g14700, F10B6.10
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 iron ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron By similarity
Metal bindingi114 – 1141Iron By similarity
Metal bindingi114 – 1141Zinc By similarity
Metal bindingi117 – 1171Iron By similarity
Metal bindingi152 – 1521Zinc By similarity
Metal bindingi246 – 2461Zinc By similarity
Active sitei255 – 2551Proton donor By similarity
Metal bindingi281 – 2811Zinc By similarity
Metal bindingi283 – 2831Iron By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G14700-MONOMER.
ARA:GQT-1524-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 3 (EC:3.1.3.2)
Gene namesi
Name:PAP3
Ordered Locus Names:At1g14700
ORF Names:F10B6.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G14700.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232 Reviewed predictionAdd
BLAST
Chaini33 – 366334Purple acid phosphatase 3PRO_0000372808Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ8H129.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ8H129.
SMRiQ8H129. Positions 74-351.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni281 – 2833Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000239892.
InParanoidiQ8H129.
KOiK01078.
OMAiRESVAKW.
PhylomeDBiQ8H129.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8H129-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTYIYRDTKI TTKSTIPFLI FFLFCFSNLS MATLKHKPVN LVFYVYNLII    50
IFSSHSSTAE LRRLLQPSKT DGTVSFLVIG DWGRRGSYNQ SQVALQMGEI 100
GEKLDIDFVI STGDNFYDNG LTSLHDPLFQ DSFTNIYTAP SLQKPWYSVL 150
GNHDYRGDVR AQLSPMLRAL DNRWVCMRSF IVNAEIVDLF FVDTTPFVDK 200
YFIQPNKHVY DWSGVLPRQT YLNNLLKELD VALRESVAKW KIVIGHHTIK 250
SAGHHGNTIE LEKHLLPILQ ANEVDLYVNG HDHCLEHISS VDSNIQFMTS 300
GGGSKAWKGG DVNYVEPEEM RFYYDGQGFM SVHVSEAELR VVFYDVFGHV 350
LHHWKKTYKE ALYFAS 366
Length:366
Mass (Da):42,052
Last modified:March 1, 2003 - v1
Checksum:i427FD0C4B474DCC0
GO
Isoform 2 (identifier: Q8H129-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-150: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:364
Mass (Da):41,840
Checksum:i192AA2367701CEC0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei149 – 1502Missing in isoform 2. VSP_037188

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY686599 mRNA. Translation: AAT95435.1.
AC006917 Genomic DNA. Translation: AAF79221.1.
CP002684 Genomic DNA. Translation: AEE29210.1.
CP002684 Genomic DNA. Translation: AEE29211.1.
AY070051 mRNA. Translation: AAL49808.2.
BT000877 mRNA. Translation: AAN41277.1.
PIRiD86281.
RefSeqiNP_001077538.1. NM_001084069.1. [Q8H129-2]
NP_172923.3. NM_101339.4. [Q8H129-1]
UniGeneiAt.28419.

Genome annotation databases

EnsemblPlantsiAT1G14700.1; AT1G14700.1; AT1G14700. [Q8H129-1]
GeneIDi838035.
KEGGiath:AT1G14700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY686599 mRNA. Translation: AAT95435.1 .
AC006917 Genomic DNA. Translation: AAF79221.1 .
CP002684 Genomic DNA. Translation: AEE29210.1 .
CP002684 Genomic DNA. Translation: AEE29211.1 .
AY070051 mRNA. Translation: AAL49808.2 .
BT000877 mRNA. Translation: AAN41277.1 .
PIRi D86281.
RefSeqi NP_001077538.1. NM_001084069.1. [Q8H129-2 ]
NP_172923.3. NM_101339.4. [Q8H129-1 ]
UniGenei At.28419.

3D structure databases

ProteinModelPortali Q8H129.
SMRi Q8H129. Positions 74-351.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G14700.1 ; AT1G14700.1 ; AT1G14700 . [Q8H129-1 ]
GeneIDi 838035.
KEGGi ath:AT1G14700.

Organism-specific databases

TAIRi AT1G14700.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000239892.
InParanoidi Q8H129.
KOi K01078.
OMAi RESVAKW.
PhylomeDBi Q8H129.

Enzyme and pathway databases

BioCyci ARA:AT1G14700-MONOMER.
ARA:GQT-1524-MONOMER.

Miscellaneous databases

PROi Q8H129.

Gene expression databases

Genevestigatori Q8H129.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PIRSFi PIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMi SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPPA3_ARATH
AccessioniPrimary (citable) accession number: Q8H129
Secondary accession number(s): Q8VYS7, Q9LQW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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