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Q8H129

- PPA3_ARATH

UniProt

Q8H129 - PPA3_ARATH

Protein

Purple acid phosphatase 3

Gene

PAP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Binds 1 iron ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi81 – 811IronBy similarity
    Metal bindingi114 – 1141IronBy similarity
    Metal bindingi114 – 1141ZincBy similarity
    Metal bindingi117 – 1171IronBy similarity
    Metal bindingi152 – 1521ZincBy similarity
    Metal bindingi246 – 2461ZincBy similarity
    Active sitei255 – 2551Proton donorBy similarity
    Metal bindingi281 – 2811ZincBy similarity
    Metal bindingi283 – 2831IronBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: TAIR
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT1G14700-MONOMER.
    ARA:GQT-1524-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase 3 (EC:3.1.3.2)
    Gene namesi
    Name:PAP3
    Ordered Locus Names:At1g14700
    ORF Names:F10B6.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G14700.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. vacuole Source: TAIR

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 366334Purple acid phosphatase 3PRO_0000372808Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed in stems, leaves, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ8H129.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H129.
    SMRiQ8H129. Positions 74-351.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni281 – 2833Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG1409.
    HOGENOMiHOG000239892.
    InParanoidiQ8H129.
    KOiK01078.
    OMAiRESVAKW.
    PhylomeDBiQ8H129.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR024927. Acid_Pase_5.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
    SUPFAMiSSF56300. SSF56300. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8H129-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTYIYRDTKI TTKSTIPFLI FFLFCFSNLS MATLKHKPVN LVFYVYNLII    50
    IFSSHSSTAE LRRLLQPSKT DGTVSFLVIG DWGRRGSYNQ SQVALQMGEI 100
    GEKLDIDFVI STGDNFYDNG LTSLHDPLFQ DSFTNIYTAP SLQKPWYSVL 150
    GNHDYRGDVR AQLSPMLRAL DNRWVCMRSF IVNAEIVDLF FVDTTPFVDK 200
    YFIQPNKHVY DWSGVLPRQT YLNNLLKELD VALRESVAKW KIVIGHHTIK 250
    SAGHHGNTIE LEKHLLPILQ ANEVDLYVNG HDHCLEHISS VDSNIQFMTS 300
    GGGSKAWKGG DVNYVEPEEM RFYYDGQGFM SVHVSEAELR VVFYDVFGHV 350
    LHHWKKTYKE ALYFAS 366
    Length:366
    Mass (Da):42,052
    Last modified:March 1, 2003 - v1
    Checksum:i427FD0C4B474DCC0
    GO
    Isoform 2 (identifier: Q8H129-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         149-150: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:364
    Mass (Da):41,840
    Checksum:i192AA2367701CEC0
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei149 – 1502Missing in isoform 2. CuratedVSP_037188

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY686599 mRNA. Translation: AAT95435.1.
    AC006917 Genomic DNA. Translation: AAF79221.1.
    CP002684 Genomic DNA. Translation: AEE29210.1.
    CP002684 Genomic DNA. Translation: AEE29211.1.
    AY070051 mRNA. Translation: AAL49808.2.
    BT000877 mRNA. Translation: AAN41277.1.
    PIRiD86281.
    RefSeqiNP_001077538.1. NM_001084069.1. [Q8H129-2]
    NP_172923.3. NM_101339.4. [Q8H129-1]
    UniGeneiAt.28419.

    Genome annotation databases

    EnsemblPlantsiAT1G14700.1; AT1G14700.1; AT1G14700. [Q8H129-1]
    GeneIDi838035.
    KEGGiath:AT1G14700.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY686599 mRNA. Translation: AAT95435.1 .
    AC006917 Genomic DNA. Translation: AAF79221.1 .
    CP002684 Genomic DNA. Translation: AEE29210.1 .
    CP002684 Genomic DNA. Translation: AEE29211.1 .
    AY070051 mRNA. Translation: AAL49808.2 .
    BT000877 mRNA. Translation: AAN41277.1 .
    PIRi D86281.
    RefSeqi NP_001077538.1. NM_001084069.1. [Q8H129-2 ]
    NP_172923.3. NM_101339.4. [Q8H129-1 ]
    UniGenei At.28419.

    3D structure databases

    ProteinModelPortali Q8H129.
    SMRi Q8H129. Positions 74-351.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G14700.1 ; AT1G14700.1 ; AT1G14700 . [Q8H129-1 ]
    GeneIDi 838035.
    KEGGi ath:AT1G14700.

    Organism-specific databases

    TAIRi AT1G14700.

    Phylogenomic databases

    eggNOGi COG1409.
    HOGENOMi HOG000239892.
    InParanoidi Q8H129.
    KOi K01078.
    OMAi RESVAKW.
    PhylomeDBi Q8H129.

    Enzyme and pathway databases

    BioCyci ARA:AT1G14700-MONOMER.
    ARA:GQT-1524-MONOMER.

    Miscellaneous databases

    PROi Q8H129.

    Gene expression databases

    Genevestigatori Q8H129.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR024927. Acid_Pase_5.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000898. Acid_Ptase_5. 1 hit.
    SUPFAMi SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
      Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
      Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
      Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
      J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPPA3_ARATH
    AccessioniPrimary (citable) accession number: Q8H129
    Secondary accession number(s): Q8VYS7, Q9LQW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3