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Protein

Purple acid phosphatase 3

Gene

PAP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811IronBy similarity
Metal bindingi114 – 1141IronBy similarity
Metal bindingi114 – 1141ZincBy similarity
Metal bindingi117 – 1171IronBy similarity
Metal bindingi152 – 1521ZincBy similarity
Metal bindingi246 – 2461ZincBy similarity
Active sitei255 – 2551Proton donorBy similarity
Metal bindingi281 – 2811ZincBy similarity
Metal bindingi283 – 2831IronBy similarity

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G14700-MONOMER.
ARA:GQT-1524-MONOMER.
ReactomeiREACT_327526. Vitamin B2 (riboflavin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 3 (EC:3.1.3.2)
Gene namesi
Name:PAP3
Ordered Locus Names:At1g14700
ORF Names:F10B6.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G14700.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB-SubCell
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 366334Purple acid phosphatase 3PRO_0000372808Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in stems, leaves, flowers and siliques.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G14700.1.

Structurei

3D structure databases

ProteinModelPortaliQ8H129.
SMRiQ8H129. Positions 74-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni281 – 2833Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000239892.
KOiK01078.
OMAiRWVCMRS.
PhylomeDBiQ8H129.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8H129-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTYIYRDTKI TTKSTIPFLI FFLFCFSNLS MATLKHKPVN LVFYVYNLII
60 70 80 90 100
IFSSHSSTAE LRRLLQPSKT DGTVSFLVIG DWGRRGSYNQ SQVALQMGEI
110 120 130 140 150
GEKLDIDFVI STGDNFYDNG LTSLHDPLFQ DSFTNIYTAP SLQKPWYSVL
160 170 180 190 200
GNHDYRGDVR AQLSPMLRAL DNRWVCMRSF IVNAEIVDLF FVDTTPFVDK
210 220 230 240 250
YFIQPNKHVY DWSGVLPRQT YLNNLLKELD VALRESVAKW KIVIGHHTIK
260 270 280 290 300
SAGHHGNTIE LEKHLLPILQ ANEVDLYVNG HDHCLEHISS VDSNIQFMTS
310 320 330 340 350
GGGSKAWKGG DVNYVEPEEM RFYYDGQGFM SVHVSEAELR VVFYDVFGHV
360
LHHWKKTYKE ALYFAS
Length:366
Mass (Da):42,052
Last modified:March 1, 2003 - v1
Checksum:i427FD0C4B474DCC0
GO
Isoform 2 (identifier: Q8H129-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-150: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:364
Mass (Da):41,840
Checksum:i192AA2367701CEC0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei149 – 1502Missing in isoform 2. CuratedVSP_037188

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY686599 mRNA. Translation: AAT95435.1.
AC006917 Genomic DNA. Translation: AAF79221.1.
CP002684 Genomic DNA. Translation: AEE29210.1.
CP002684 Genomic DNA. Translation: AEE29211.1.
AY070051 mRNA. Translation: AAL49808.2.
BT000877 mRNA. Translation: AAN41277.1.
PIRiD86281.
RefSeqiNP_001077538.1. NM_001084069.1. [Q8H129-2]
NP_172923.3. NM_101339.4. [Q8H129-1]
UniGeneiAt.28419.

Genome annotation databases

EnsemblPlantsiAT1G14700.1; AT1G14700.1; AT1G14700. [Q8H129-1]
GeneIDi838035.
KEGGiath:AT1G14700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY686599 mRNA. Translation: AAT95435.1.
AC006917 Genomic DNA. Translation: AAF79221.1.
CP002684 Genomic DNA. Translation: AEE29210.1.
CP002684 Genomic DNA. Translation: AEE29211.1.
AY070051 mRNA. Translation: AAL49808.2.
BT000877 mRNA. Translation: AAN41277.1.
PIRiD86281.
RefSeqiNP_001077538.1. NM_001084069.1. [Q8H129-2]
NP_172923.3. NM_101339.4. [Q8H129-1]
UniGeneiAt.28419.

3D structure databases

ProteinModelPortaliQ8H129.
SMRiQ8H129. Positions 74-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G14700.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G14700.1; AT1G14700.1; AT1G14700. [Q8H129-1]
GeneIDi838035.
KEGGiath:AT1G14700.

Organism-specific databases

TAIRiAT1G14700.

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000239892.
KOiK01078.
OMAiRWVCMRS.
PhylomeDBiQ8H129.

Enzyme and pathway databases

BioCyciARA:AT1G14700-MONOMER.
ARA:GQT-1524-MONOMER.
ReactomeiREACT_327526. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

PROiQ8H129.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPPA3_ARATH
AccessioniPrimary (citable) accession number: Q8H129
Secondary accession number(s): Q8VYS7, Q9LQW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2003
Last modified: July 22, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.