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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2

Gene

MNS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Class I alpha-mannosidase essential for early N-glycan processing. Progressively trim alpha-1,2-linked mannose residues. Produces Man5GlcNAc2 from Man8GlcNAc2, but only Man6GlcNAc2 from Man9GlcNAc2. Have difficulty acting on the terminal mannose of the b-branch. Involved in root development and cell wall biosynthesis.2 Publications

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.1 Publication

Cofactori

Ca2+1 Publication, Mn2+1 Publication, Mg2+1 PublicationNote: Ca2+ or Mn2+. Mg2+ can be used to a lesser extent.1 Publication

Enzyme regulationi

Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine.2 Publications

pH dependencei

Optimum pH is 7.0. Stable from pH 5.0 to 10.0.1 Publication

Temperature dependencei

Optimum temperature is 25 degrees Celsius.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei180Proton donorBy similarity1
Active sitei312By similarity1
Active sitei424Proton donorBy similarity1
Active sitei446By similarity1
Metal bindingi530CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • N-glycan processing Source: TAIR
  • protein glycosylation Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
LigandCalcium, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G21160-MONOMER
ReactomeiR-ATH-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2
UniPathwayiUPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2 (EC:3.2.1.1131 Publication)
Short name:
AtMANIa
Alternative name(s):
Alpha-mannosidase IA
Gene namesi
Name:MNS2
Synonyms:MANIA
Ordered Locus Names:At3g21160
ORF Names:MSA6.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G21160
TAIRilocus:2092965 AT3G21160

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 27CytoplasmicSequence analysisAdd BLAST27
Transmembranei28 – 45Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini46 – 572LumenalSequence analysisAdd BLAST527

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; due the redundancy with MNS1. Lack of complex N-glycans, shorter roots and increased lateral root formation in mns1 and mns2 double mutants.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003979341 – 572Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2Add BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi326N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi377 ↔ 410By similarity
Glycosylationi460N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8H116

Expressioni

Tissue specificityi

Expressed in flowers, siliques, stems, leaves, roots, pollen grains and shoot apical meristems.2 Publications

Gene expression databases

ExpressionAtlasiQ8H116 baseline and differential
GenevisibleiQ8H116 AT

Interactioni

Protein-protein interaction databases

BioGridi7000, 1 interactor
IntActiQ8H116, 1 interactor
STRINGi3702.AT3G21160.1

Structurei

3D structure databases

ProteinModelPortaliQ8H116
SMRiQ8H116
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili51 – 81Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2204 Eukaryota
ENOG410XP04 LUCA
HOGENOMiHOG000181987
InParanoidiQ8H116
KOiK01230
OMAiDHKPGPG
OrthoDBiEOG09360BX2
PhylomeDBiQ8H116

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequencei

Sequence statusi: Complete.

Q8H116-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARNKLVSGS HGIWKYFNPA FYLRRPRRLA LLIILFVSVS MVVWDRQSLS
60 70 80 90 100
RDYQFEVSKL NEEVLRLQQM LEEIKSVTED VSVNSLKDVQ EDPVDAQRMQ
110 120 130 140 150
RVKEAMVHAW SSYEKYAWGQ DELQPQTKDG VDSFGGLGAT MIDALDTLYI
160 170 180 190 200
MGLDEQFQKA REWVASSLDF DKDYAASMFE TTIRVVGGLL SAYDLSGDKI
210 220 230 240 250
FLEKAMDIAD RLLPAWDTQS GIPYNIINLK HGNAHNPTWA GGDSILADSG
260 270 280 290 300
TEQLEFIALS QRTGDPKYQQ KVEKVISVLN KNFPADGLLP IYINPDTANP
310 320 330 340 350
SQSTITFGAM GDSFYEYLLK VWVFGNKTSA VKHYRDMWEK SMNGLLSLVK
360 370 380 390 400
KSTPLSFTYI CEKSGNSLID KMDELACFAP GMLALGASGY SDPAEGKKFL
410 420 430 440 450
TLAEELAWTC YNFYQSTPTK LAGENYFFNS GSDMSVGTSW NILRPETVES
460 470 480 490 500
LFYLWRLTGN KTYQEWGWNI FEAFEKNSRI ESGYVGLKDV NTGVKDNKMQ
510 520 530 540 550
SFFLAETLKY LYLLFSPTTV IPLDEWVFNT EAHPLKIKSR NDQVNLKQSN
560 570
KVLLRKPAFR IRQRHYGRIT KK
Length:572
Mass (Da):65,136
Last modified:March 1, 2003 - v1
Checksum:i14614DD4525F9499
GO

Sequence cautioni

The sequence BAB01459 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113Y → H in AAK92711 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000604, AB023045 Genomic DNA Translation: BAB01459.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE76468.1
AY050776 mRNA Translation: AAK92711.1
BT000893 mRNA Translation: AAN41293.1
RefSeqiNP_566675.1, NM_113010.3
UniGeneiAt.25787

Genome annotation databases

EnsemblPlantsiAT3G21160.1; AT3G21160.1; AT3G21160
GeneIDi821668
GrameneiAT3G21160.1; AT3G21160.1; AT3G21160
KEGGiath:AT3G21160

Similar proteinsi

Entry informationi

Entry nameiMNS2_ARATH
AccessioniPrimary (citable) accession number: Q8H116
Secondary accession number(s): Q94A04, Q9LJB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health