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Q8H116

- MNS2_ARATH

UniProt

Q8H116 - MNS2_ARATH

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Protein
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2
Gene
MNS2, MANIA, At3g21160, MSA6.31
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Class I alpha-mannosidase essential for early N-glycan processing. Progressively trim alpha-1,2-linked mannose residues. Produces Man5GlcNAc2 from Man8GlcNAc2, but only Man6GlcNAc2 from Man9GlcNAc2. Have difficulty acting on the terminal mannose of the b-branch. Involved in root development and cell wall biosynthesis.2 Publications

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.1 Publication

Cofactori

Calcium or manganese. Magnesium can be used to a lesser extent.

Enzyme regulationi

Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine.2 Publications

pH dependencei

Optimum pH is 7.0. Stable from pH 5.0 to 10.0.1 Publication

Temperature dependencei

Optimum temperature is 25 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei180 – 1801Proton donor By similarity
Active sitei312 – 3121 By similarity
Active sitei446 – 4461 By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: TAIR
  2. calcium ion binding Source: InterPro
  3. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. N-glycan processing Source: TAIR
  2. root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Magnesium, Manganese

Enzyme and pathway databases

BioCyciARA:AT3G21160-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2 (EC:3.2.1.113)
Short name:
AtMANIa
Alternative name(s):
Alpha-mannosidase IA
Gene namesi
Name:MNS2
Synonyms:MANIA
Ordered Locus Names:At3g21160
ORF Names:MSA6.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G21160.

Subcellular locationi

Golgi apparatus membrane; Single-pass type II membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei28 – 4518Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini46 – 572527Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. Golgi membrane Source: UniProtKB-SubCell
  3. endosome Source: TAIR
  4. integral component of membrane Source: UniProtKB-KW
  5. trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; due the redundancy with MNS1. Lack of complex N-glycans, shorter roots and increased lateral root formation in mns1 and mns2 double mutants.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2
PRO_0000397934Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi326 – 3261N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi377 ↔ 410 By similarity
Glycosylationi460 – 4601N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8H116.
PRIDEiQ8H116.

Expressioni

Tissue specificityi

Expressed in flowers, siliques, stems, leaves, roots, pollen grains and shoot apical meristems.2 Publications

Gene expression databases

GenevestigatoriQ8H116.

Interactioni

Protein-protein interaction databases

BioGridi7000. 1 interaction.
IntActiQ8H116. 1 interaction.
STRINGi3702.AT3G21160.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8H116.
SMRiQ8H116. Positions 98-537.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili51 – 8131 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300315.
HOGENOMiHOG000181987.
InParanoidiQ8H116.
KOiK01230.
OMAiTSAVKHY.
PhylomeDBiQ8H116.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8H116-1 [UniParc]FASTAAdd to Basket

« Hide

MARNKLVSGS HGIWKYFNPA FYLRRPRRLA LLIILFVSVS MVVWDRQSLS    50
RDYQFEVSKL NEEVLRLQQM LEEIKSVTED VSVNSLKDVQ EDPVDAQRMQ 100
RVKEAMVHAW SSYEKYAWGQ DELQPQTKDG VDSFGGLGAT MIDALDTLYI 150
MGLDEQFQKA REWVASSLDF DKDYAASMFE TTIRVVGGLL SAYDLSGDKI 200
FLEKAMDIAD RLLPAWDTQS GIPYNIINLK HGNAHNPTWA GGDSILADSG 250
TEQLEFIALS QRTGDPKYQQ KVEKVISVLN KNFPADGLLP IYINPDTANP 300
SQSTITFGAM GDSFYEYLLK VWVFGNKTSA VKHYRDMWEK SMNGLLSLVK 350
KSTPLSFTYI CEKSGNSLID KMDELACFAP GMLALGASGY SDPAEGKKFL 400
TLAEELAWTC YNFYQSTPTK LAGENYFFNS GSDMSVGTSW NILRPETVES 450
LFYLWRLTGN KTYQEWGWNI FEAFEKNSRI ESGYVGLKDV NTGVKDNKMQ 500
SFFLAETLKY LYLLFSPTTV IPLDEWVFNT EAHPLKIKSR NDQVNLKQSN 550
KVLLRKPAFR IRQRHYGRIT KK 572
Length:572
Mass (Da):65,136
Last modified:March 1, 2003 - v1
Checksum:i14614DD4525F9499
GO

Sequence cautioni

The sequence BAB01459.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131Y → H in AAK92711. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP000604, AB023045 Genomic DNA. Translation: BAB01459.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76468.1.
AY050776 mRNA. Translation: AAK92711.1.
BT000893 mRNA. Translation: AAN41293.1.
RefSeqiNP_566675.1. NM_113010.2.
UniGeneiAt.25787.

Genome annotation databases

EnsemblPlantsiAT3G21160.1; AT3G21160.1; AT3G21160.
GeneIDi821668.
KEGGiath:AT3G21160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP000604 , AB023045 Genomic DNA. Translation: BAB01459.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE76468.1 .
AY050776 mRNA. Translation: AAK92711.1 .
BT000893 mRNA. Translation: AAN41293.1 .
RefSeqi NP_566675.1. NM_113010.2.
UniGenei At.25787.

3D structure databases

ProteinModelPortali Q8H116.
SMRi Q8H116. Positions 98-537.
ModBasei Search...

Protein-protein interaction databases

BioGridi 7000. 1 interaction.
IntActi Q8H116. 1 interaction.
STRINGi 3702.AT3G21160.1-P.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

Proteomic databases

PaxDbi Q8H116.
PRIDEi Q8H116.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G21160.1 ; AT3G21160.1 ; AT3G21160 .
GeneIDi 821668.
KEGGi ath:AT3G21160.

Organism-specific databases

TAIRi AT3G21160.

Phylogenomic databases

eggNOGi NOG300315.
HOGENOMi HOG000181987.
InParanoidi Q8H116.
KOi K01230.
OMAi TSAVKHY.
PhylomeDBi Q8H116.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci ARA:AT3G21160-MONOMER.

Gene expression databases

Genevestigatori Q8H116.

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana."
    Liebminger E., Huttner S., Vavra U., Fischl R., Schoberer J., Grass J., Blaukopf C., Seifert G.J., Altmann F., Mach L., Strasser R.
    Plant Cell 21:3850-3867(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, DISRUPTION PHENOTYPE.
  6. "Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation."
    Kajiura H., Koiwa H., Nakazawa Y., Okazawa A., Kobayashi A., Seki T., Fujiyama K.
    Glycobiology 20:235-247(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMNS2_ARATH
AccessioniPrimary (citable) accession number: Q8H116
Secondary accession number(s): Q94A04, Q9LJB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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