Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2 - Arabidopsis thaliana (Mouse-ear cress)

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Q8H116 (MNS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 61. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2
Short name=AtMANIa
EC=3.2.1.113

Alternative name(s):
Alpha-mannosidase IA

Gene names

Name:	MNS2
Synonyms:	MANIA
Ordered Locus Names:	At3g21160
ORF Names:	MSA6.31

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Class I alpha-mannosidase essential for early N-glycan processing. Progressively trim alpha-1,2-linked mannose residues. Produces Man₅GlcNAc₂ from Man₈GlcNAc₂, but only Man₆GlcNAc₂ from Man₉GlcNAc₂. Have difficulty acting on the terminal mannose of the b-branch. Involved in root development and cell wall biosynthesis. Ref.5 Ref.6
Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man₉(GlcNAc)₂. Ref.6
Cofactor	Calcium or manganese. Magnesium can be used to a lesser extent.
Enzyme regulation	Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine. Ref.5 Ref.6
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Ref.5 Ref.6.
Tissue specificity	Expressed in flowers, siliques, stems, leaves, roots, pollen grains and shoot apical meristems. Ref.5 Ref.6
Disruption phenotype	No visible phenotype, due the redundancy with MNS1. Lack of complex N-glycans, shorter roots and increased lateral root formation in double mutants mns1 mns2. Ref.5 Ref.6
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0. Stable from pH 5.0 to 10.0. Ref.6 Temperature dependence: Optimum temperature is 25 degrees Celsius.
Sequence caution	The sequence BAB01459.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
Ligand	Calcium Magnesium Manganese
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-glycan processing Inferred from mutant phenotype Ref.5. Source: TAIR root development Inferred from genetic interaction Ref.5. Source: TAIR
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 16618929 Ref.6 Ref.5 PubMed 22430844 PubMed 22923678. Source: TAIR Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from direct assay PubMed 22923678. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW trans-Golgi network Inferred from direct assay PubMed 22923678. Source: TAIR
Molecular_function	alpha-mannosidase activity Inferred from direct assay Ref.6 Ref.5. Source: TAIR calcium ion binding Inferred from electronic annotation. Source: InterPro mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 572

572

Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2

PRO_0000397934

Regions

Topological domain

1 – 27

Cytoplasmic Potential

Transmembrane

28 – 45

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

46 – 572

527

Lumenal Potential

Coiled coil

51 – 81

Potential

Sites

Active site

180

Proton donor By similarity

Active site

312

By similarity

Active site

446

By similarity

Amino acid modifications

Glycosylation

326

N-linked (GlcNAc...) Potential

Glycosylation

460

N-linked (GlcNAc...) Potential

Disulfide bond

377 ↔ 410

By similarity

Experimental info

Sequence conflict

113

Y → H in AAK92711. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q8H116 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 14614DD4525F9499
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FASTA

572

65,136

        10         20         30         40         50         60 
MARNKLVSGS HGIWKYFNPA FYLRRPRRLA LLIILFVSVS MVVWDRQSLS RDYQFEVSKL 

        70         80         90        100        110        120 
NEEVLRLQQM LEEIKSVTED VSVNSLKDVQ EDPVDAQRMQ RVKEAMVHAW SSYEKYAWGQ 

       130        140        150        160        170        180 
DELQPQTKDG VDSFGGLGAT MIDALDTLYI MGLDEQFQKA REWVASSLDF DKDYAASMFE 

       190        200        210        220        230        240 
TTIRVVGGLL SAYDLSGDKI FLEKAMDIAD RLLPAWDTQS GIPYNIINLK HGNAHNPTWA 

       250        260        270        280        290        300 
GGDSILADSG TEQLEFIALS QRTGDPKYQQ KVEKVISVLN KNFPADGLLP IYINPDTANP 

       310        320        330        340        350        360 
SQSTITFGAM GDSFYEYLLK VWVFGNKTSA VKHYRDMWEK SMNGLLSLVK KSTPLSFTYI 

       370        380        390        400        410        420 
CEKSGNSLID KMDELACFAP GMLALGASGY SDPAEGKKFL TLAEELAWTC YNFYQSTPTK 

       430        440        450        460        470        480 
LAGENYFFNS GSDMSVGTSW NILRPETVES LFYLWRLTGN KTYQEWGWNI FEAFEKNSRI 

       490        500        510        520        530        540 
ESGYVGLKDV NTGVKDNKMQ SFFLAETLKY LYLLFSPTTV IPLDEWVFNT EAHPLKIKSR 

       550        560        570 
NDQVNLKQSN KVLLRKPAFR IRQRHYGRIT KK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana." Liebminger E., Huttner S., Vavra U., Fischl R., Schoberer J., Grass J., Blaukopf C., Seifert G.J., Altmann F., Mach L., Strasser R. Plant Cell 21:3850-3867(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, DISRUPTION PHENOTYPE.
[6]	"Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation." Kajiura H., Koiwa H., Nakazawa Y., Okazawa A., Kobayashi A., Seki T., Fujiyama K. Glycobiology 20:235-247(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000604, AB023045 Genomic DNA. Translation: BAB01459.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE76468.1. AY050776 mRNA. Translation: AAK92711.1. BT000893 mRNA. Translation: AAN41293.1.
IPI	IPI00534039.
RefSeq	NP_566675.1. NM_113010.2.
UniGene	At.25787.
3D structure databases
HSSP	HSSP built from PDB template 1FMI based on UniProtKB Q9UKM7.
ProteinModelPortal	Q8H116.
SMR	Q8H116. Positions 98-537.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8H116. 1 interaction.
STRING	3702.AT3G21160.1-P.
Protein family/group databases
CAZy	GH47. Glycoside Hydrolase Family 47.
Proteomic databases
PaxDb	Q8H116.
PRIDE	Q8H116.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G21160.1; AT3G21160.1; AT3G21160.
GeneID	821668.
KEGG	ath:AT3G21160.
Organism-specific databases
TAIR	At3g21160.
Phylogenomic databases
eggNOG	NOG300315.
HOGENOM	HOG000181987.
InParanoid	Q8H116.
KO	K01230.
OMA	ENYFFHT.
PhylomeDB	Q8H116.
ProtClustDB	CLSN2914679.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	Q8H116.
Genevestigator	Q8H116.
Family and domain databases
Gene3D	1.50.10.50. 1 hit.
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
PANTHER	PTHR11742. PTHR11742. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
SUPFAM	SSF48225. Glyco_hydro_47. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNS2_ARATH

Accession

Primary (citable) accession number: Q8H116
Secondary accession number(s): Q94A04, Q9LJB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents