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Q8H116

- MNS2_ARATH

UniProt

Q8H116 - MNS2_ARATH

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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2

Gene

MNS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Class I alpha-mannosidase essential for early N-glycan processing. Progressively trim alpha-1,2-linked mannose residues. Produces Man5GlcNAc2 from Man8GlcNAc2, but only Man6GlcNAc2 from Man9GlcNAc2. Have difficulty acting on the terminal mannose of the b-branch. Involved in root development and cell wall biosynthesis.2 Publications

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.1 Publication

Cofactori

Ca2+, Mn2+, Mg2+Note: Ca(2+) or Mn(2+). Mg(2+) can be used to a lesser extent.

Enzyme regulationi

Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine.2 Publications

pH dependencei

Optimum pH is 7.0. Stable from pH 5.0 to 10.0.1 Publication

Temperature dependencei

Optimum temperature is 25 degrees Celsius.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei180 – 1801Proton donorBy similarity
Active sitei312 – 3121By similarity
Active sitei446 – 4461By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: TAIR
  2. calcium ion binding Source: InterPro
  3. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. N-glycan processing Source: TAIR
  2. root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Magnesium, Manganese

Enzyme and pathway databases

BioCyciARA:AT3G21160-MONOMER.
ReactomeiREACT_250137. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2 (EC:3.2.1.113)
Short name:
AtMANIa
Alternative name(s):
Alpha-mannosidase IA
Gene namesi
Name:MNS2
Synonyms:MANIA
Ordered Locus Names:At3g21160
ORF Names:MSA6.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G21160.

Subcellular locationi

Golgi apparatus membrane 2 Publications; Single-pass type II membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei28 – 4518Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini46 – 572527LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endosome Source: TAIR
  2. Golgi apparatus Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
  4. trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; due the redundancy with MNS1. Lack of complex N-glycans, shorter roots and increased lateral root formation in mns1 and mns2 double mutants.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2PRO_0000397934Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi377 ↔ 410By similarity
Glycosylationi460 – 4601N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8H116.
PRIDEiQ8H116.

Expressioni

Tissue specificityi

Expressed in flowers, siliques, stems, leaves, roots, pollen grains and shoot apical meristems.2 Publications

Gene expression databases

ExpressionAtlasiQ8H116. baseline and differential.
GenevestigatoriQ8H116.

Interactioni

Protein-protein interaction databases

BioGridi7000. 1 interaction.
IntActiQ8H116. 1 interaction.
STRINGi3702.AT3G21160.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8H116.
SMRiQ8H116. Positions 98-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili51 – 8131Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300315.
HOGENOMiHOG000181987.
InParanoidiQ8H116.
KOiK01230.
OMAiTSAVKHY.
PhylomeDBiQ8H116.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8H116-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARNKLVSGS HGIWKYFNPA FYLRRPRRLA LLIILFVSVS MVVWDRQSLS
60 70 80 90 100
RDYQFEVSKL NEEVLRLQQM LEEIKSVTED VSVNSLKDVQ EDPVDAQRMQ
110 120 130 140 150
RVKEAMVHAW SSYEKYAWGQ DELQPQTKDG VDSFGGLGAT MIDALDTLYI
160 170 180 190 200
MGLDEQFQKA REWVASSLDF DKDYAASMFE TTIRVVGGLL SAYDLSGDKI
210 220 230 240 250
FLEKAMDIAD RLLPAWDTQS GIPYNIINLK HGNAHNPTWA GGDSILADSG
260 270 280 290 300
TEQLEFIALS QRTGDPKYQQ KVEKVISVLN KNFPADGLLP IYINPDTANP
310 320 330 340 350
SQSTITFGAM GDSFYEYLLK VWVFGNKTSA VKHYRDMWEK SMNGLLSLVK
360 370 380 390 400
KSTPLSFTYI CEKSGNSLID KMDELACFAP GMLALGASGY SDPAEGKKFL
410 420 430 440 450
TLAEELAWTC YNFYQSTPTK LAGENYFFNS GSDMSVGTSW NILRPETVES
460 470 480 490 500
LFYLWRLTGN KTYQEWGWNI FEAFEKNSRI ESGYVGLKDV NTGVKDNKMQ
510 520 530 540 550
SFFLAETLKY LYLLFSPTTV IPLDEWVFNT EAHPLKIKSR NDQVNLKQSN
560 570
KVLLRKPAFR IRQRHYGRIT KK
Length:572
Mass (Da):65,136
Last modified:March 1, 2003 - v1
Checksum:i14614DD4525F9499
GO

Sequence cautioni

The sequence BAB01459.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131Y → H in AAK92711. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000604, AB023045 Genomic DNA. Translation: BAB01459.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76468.1.
AY050776 mRNA. Translation: AAK92711.1.
BT000893 mRNA. Translation: AAN41293.1.
RefSeqiNP_566675.1. NM_113010.2.
UniGeneiAt.25787.

Genome annotation databases

EnsemblPlantsiAT3G21160.1; AT3G21160.1; AT3G21160.
GeneIDi821668.
KEGGiath:AT3G21160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000604 , AB023045 Genomic DNA. Translation: BAB01459.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE76468.1 .
AY050776 mRNA. Translation: AAK92711.1 .
BT000893 mRNA. Translation: AAN41293.1 .
RefSeqi NP_566675.1. NM_113010.2.
UniGenei At.25787.

3D structure databases

ProteinModelPortali Q8H116.
SMRi Q8H116. Positions 98-537.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 7000. 1 interaction.
IntActi Q8H116. 1 interaction.
STRINGi 3702.AT3G21160.1-P.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

Proteomic databases

PaxDbi Q8H116.
PRIDEi Q8H116.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G21160.1 ; AT3G21160.1 ; AT3G21160 .
GeneIDi 821668.
KEGGi ath:AT3G21160.

Organism-specific databases

TAIRi AT3G21160.

Phylogenomic databases

eggNOGi NOG300315.
HOGENOMi HOG000181987.
InParanoidi Q8H116.
KOi K01230.
OMAi TSAVKHY.
PhylomeDBi Q8H116.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci ARA:AT3G21160-MONOMER.
Reactomei REACT_250137. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

Gene expression databases

ExpressionAtlasi Q8H116. baseline and differential.
Genevestigatori Q8H116.

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana."
    Liebminger E., Huttner S., Vavra U., Fischl R., Schoberer J., Grass J., Blaukopf C., Seifert G.J., Altmann F., Mach L., Strasser R.
    Plant Cell 21:3850-3867(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, DISRUPTION PHENOTYPE.
  6. "Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation."
    Kajiura H., Koiwa H., Nakazawa Y., Okazawa A., Kobayashi A., Seki T., Fujiyama K.
    Glycobiology 20:235-247(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMNS2_ARATH
AccessioniPrimary (citable) accession number: Q8H116
Secondary accession number(s): Q94A04, Q9LJB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3