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Protein

Glucose-6-phosphate isomerase 1, chloroplastic

Gene

PGI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the synthesis of starch in leaves.1 Publication

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Enzyme regulationi

Inhibited by glycerol-3-P (G3P).1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei392 – 3921Proton donorBy similarity
Active sitei421 – 4211By similarity
Active sitei526 – 5261By similarity

GO - Molecular functioni

  1. glucose-6-phosphate isomerase activity Source: TAIR

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
  2. glycolytic process Source: UniProtKB-UniPathway
  3. positive regulation of flower development Source: TAIR
  4. starch metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciMetaCyc:AT4G24620-MONOMER.
UniPathwayiUPA00109; UER00181.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase 1, chloroplastic (EC:5.3.1.9)
Short name:
GPI 1
Alternative name(s):
Phosphoglucose isomerase 1
Short name:
PGI 1
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:PGI1
Synonyms:pgi2, pgi3
Ordered Locus Names:At4g24620
ORF Names:F22K18.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G24620.

Subcellular locationi

Plastidchloroplast stroma 5 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. cytosol Source: TAIR
  5. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi166 – 1661S → F in pgi1-1; decreased plastid phospho-glucose (Glc) isomerase activity leading to a deficiency in leaf starch synthesis, but an accumulation of starch in root cap cells. Delayed flowering time under short-day conditions. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848ChloroplastSequence AnalysisAdd
BLAST
Chaini49 – 613565Glucose-6-phosphate isomerase 1, chloroplasticPRO_0000420249Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei595 – 5951Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ8H103.

Expressioni

Gene expression databases

GenevestigatoriQ8H103.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G24620.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8H103.
SMRiQ8H103. Positions 32-545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0166.
HOGENOMiHOG000131425.
InParanoidiQ8H103.
KOiK01810.
OMAiRQVGHYW.
PhylomeDBiQ8H103.

Family and domain databases

HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 2 hits.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8H103-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLSGLYSS SPSLKPAKNH SFKALPAQSR DSFSFPHTSK PTNLPLTLSS
60 70 80 90 100
ARSVARDISH ADSKKELLKD PDALWKRYLD WFYQQKELGL YLDISRVGFT
110 120 130 140 150
DEFVAEMEPR FQAAFKAMED LEKGSIANPD EGRMVGHYWL RNSKLAPKPT
160 170 180 190 200
LKTLIENTLD SICAFSDDII SGKIKPPSSP EGRFTQILSV GIGGSALGPQ
210 220 230 240 250
FVAEALAPDN PPLKIRFIDN TDPAGIDHQI AQLGPELAST LVVVISKSGG
260 270 280 290 300
TPETRNGLLE VQKAFREAGL NFAKQGVAIT QENSLLDNTA RIEGWLARFP
310 320 330 340 350
MYDWVGGRTS IMSAVGLLPA ALQGINVREM LTGAALMDEA TRTTSIKNNP
360 370 380 390 400
AALLAMCWYW ASNGVGSKDM VVLPYKDSLL LFSRYLQQLV MESLGKEFDL
410 420 430 440 450
DGNTVNQGLT VYGNKGSTDQ HAYIQQLRDG VHNFFATFIE VLRDRPPGHD
460 470 480 490 500
WELEPGVTCG DYLFGMLQGT RSALYANGRE SISVTIQEVT PTSVGAIIAL
510 520 530 540 550
YERAVGLYAS IVNINAYHQP GVEAGKKAAA EVLALQKRVL SVLNEATCKD
560 570 580 590 600
PVEPLTLEEI ADRCHAPEEI EMIYKIIAHM SANDRVLIAE GNCGSPRSIK
610
VYLGECNVDD LYA
Length:613
Mass (Da):67,048
Last modified:March 1, 2003 - v1
Checksum:i95792BA6A173991B
GO
Isoform 2 (identifier: Q8H103-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     570-570: I → K
     571-613: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:570
Mass (Da):62,308
Checksum:i8B90C7BF2FBC24DA
GO

Sequence cautioni

The sequence CAA23001.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79372.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 4522ALPAQ…PTNLP → GIAGAISRFLLFPTYLQTHQ ST in AAF24124 (PubMed:10806248).CuratedAdd
BLAST
Sequence conflicti201 – 2077FVAEALA → LSLRLG in AAF24124 (PubMed:10806248).Curated
Sequence conflicti321 – 3211A → H in AAF24124 (PubMed:10806248).Curated
Sequence conflicti422 – 4221A → P in AAF24124 (PubMed:10806248).Curated
Sequence conflicti444 – 4441D → Y in AAF24124 (PubMed:10806248).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei570 – 5701I → K in isoform 2. CuratedVSP_044431
Alternative sequencei571 – 61343Missing in isoform 2. CuratedVSP_044432Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF120494 Genomic DNA. Translation: AAF24124.1.
AL035356 Genomic DNA. Translation: CAA23001.1. Sequence problems.
AL161561 Genomic DNA. Translation: CAB79372.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84933.1.
CP002687 Genomic DNA. Translation: AEE84934.1.
BT000953 mRNA. Translation: AAN41353.1.
AK227111 mRNA. Translation: BAE99162.1.
PIRiT05572.
RefSeqiNP_001078444.1. NM_001084975.1. [Q8H103-2]
NP_194193.2. NM_118595.4. [Q8H103-1]
UniGeneiAt.25158.

Genome annotation databases

EnsemblPlantsiAT4G24620.1; AT4G24620.1; AT4G24620. [Q8H103-1]
GeneIDi828564.
KEGGiath:AT4G24620.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF120494 Genomic DNA. Translation: AAF24124.1.
AL035356 Genomic DNA. Translation: CAA23001.1. Sequence problems.
AL161561 Genomic DNA. Translation: CAB79372.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84933.1.
CP002687 Genomic DNA. Translation: AEE84934.1.
BT000953 mRNA. Translation: AAN41353.1.
AK227111 mRNA. Translation: BAE99162.1.
PIRiT05572.
RefSeqiNP_001078444.1. NM_001084975.1. [Q8H103-2]
NP_194193.2. NM_118595.4. [Q8H103-1]
UniGeneiAt.25158.

3D structure databases

ProteinModelPortaliQ8H103.
SMRiQ8H103. Positions 32-545.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G24620.1-P.

Proteomic databases

PRIDEiQ8H103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G24620.1; AT4G24620.1; AT4G24620. [Q8H103-1]
GeneIDi828564.
KEGGiath:AT4G24620.

Organism-specific databases

TAIRiAT4G24620.

Phylogenomic databases

eggNOGiCOG0166.
HOGENOMiHOG000131425.
InParanoidiQ8H103.
KOiK01810.
OMAiRQVGHYW.
PhylomeDBiQ8H103.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
UPA00138.
BioCyciMetaCyc:AT4G24620-MONOMER.

Gene expression databases

GenevestigatoriQ8H103.

Family and domain databases

HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 2 hits.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mutation of Arabidopsis plastid phosphoglucose isomerase affects leaf starch synthesis and floral initiation."
    Yu T.-S., Lue W.-L., Wang S.-M., Chen J.
    Plant Physiol. 123:319-326(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF SER-166.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
    Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
    Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: MASS SPECTROMETRY [LARGE SCALE ANALYSIS], PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  9. "SUGAR-DEPENDENT6 encodes a mitochondrial flavin adenine dinucleotide-dependent glycerol-3-p dehydrogenase, which is required for glycerol catabolism and post germinative seedling growth in Arabidopsis."
    Quettier A.-L., Shaw E., Eastmond P.J.
    Plant Physiol. 148:519-528(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  10. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiG6PIP_ARATH
AccessioniPrimary (citable) accession number: Q8H103
Secondary accession number(s): A8MR16, Q9SB57, Q9SEJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.