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Q8H103

- G6PIP_ARATH

UniProt

Q8H103 - G6PIP_ARATH

Protein

Glucose-6-phosphate isomerase 1, chloroplastic

Gene

PGI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Promotes the synthesis of starch in leaves.1 Publication

    Catalytic activityi

    D-glucose 6-phosphate = D-fructose 6-phosphate.

    Enzyme regulationi

    Inhibited by glycerol-3-P (G3P).1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei392 – 3921Proton donorBy similarity
    Active sitei421 – 4211By similarity
    Active sitei526 – 5261By similarity

    GO - Molecular functioni

    1. glucose-6-phosphate isomerase activity Source: TAIR

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-UniPathway
    2. glycolytic process Source: UniProtKB-UniPathway
    3. positive regulation of flower development Source: TAIR
    4. starch metabolic process Source: TAIR

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Gluconeogenesis, Glycolysis

    Enzyme and pathway databases

    BioCyciMetaCyc:AT4G24620-MONOMER.
    UniPathwayiUPA00109; UER00181.
    UPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate isomerase 1, chloroplastic (EC:5.3.1.9)
    Short name:
    GPI 1
    Alternative name(s):
    Phosphoglucose isomerase 1
    Short name:
    PGI 1
    Phosphohexose isomerase
    Short name:
    PHI
    Gene namesi
    Name:PGI1
    Synonyms:pgi2, pgi3
    Ordered Locus Names:At4g24620
    ORF Names:F22K18.180
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G24620.

    Subcellular locationi

    Plastidchloroplast stroma 5 Publications

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. chloroplast stroma Source: TAIR
    4. cytosol Source: TAIR
    5. plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi166 – 1661S → F in pgi1-1; decreased plastid phospho-glucose (Glc) isomerase activity leading to a deficiency in leaf starch synthesis, but an accumulation of starch in root cap cells. Delayed flowering time under short-day conditions. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4848ChloroplastSequence AnalysisAdd
    BLAST
    Chaini49 – 613565Glucose-6-phosphate isomerase 1, chloroplasticPRO_0000420249Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei595 – 5951Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ8H103.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8H103.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G24620.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H103.
    SMRiQ8H103. Positions 83-545.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GPI family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0166.
    HOGENOMiHOG000131425.
    InParanoidiQ8H103.
    KOiK01810.
    OMAiFTDVLWI.
    PhylomeDBiQ8H103.

    Family and domain databases

    HAMAPiMF_00473. G6P_isomerase.
    InterProiIPR001672. G6P_Isomerase.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view]
    PANTHERiPTHR11469. PTHR11469. 1 hit.
    PfamiPF00342. PGI. 2 hits.
    [Graphical view]
    PRINTSiPR00662. G6PISOMERASE.
    PROSITEiPS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8H103-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASLSGLYSS SPSLKPAKNH SFKALPAQSR DSFSFPHTSK PTNLPLTLSS    50
    ARSVARDISH ADSKKELLKD PDALWKRYLD WFYQQKELGL YLDISRVGFT 100
    DEFVAEMEPR FQAAFKAMED LEKGSIANPD EGRMVGHYWL RNSKLAPKPT 150
    LKTLIENTLD SICAFSDDII SGKIKPPSSP EGRFTQILSV GIGGSALGPQ 200
    FVAEALAPDN PPLKIRFIDN TDPAGIDHQI AQLGPELAST LVVVISKSGG 250
    TPETRNGLLE VQKAFREAGL NFAKQGVAIT QENSLLDNTA RIEGWLARFP 300
    MYDWVGGRTS IMSAVGLLPA ALQGINVREM LTGAALMDEA TRTTSIKNNP 350
    AALLAMCWYW ASNGVGSKDM VVLPYKDSLL LFSRYLQQLV MESLGKEFDL 400
    DGNTVNQGLT VYGNKGSTDQ HAYIQQLRDG VHNFFATFIE VLRDRPPGHD 450
    WELEPGVTCG DYLFGMLQGT RSALYANGRE SISVTIQEVT PTSVGAIIAL 500
    YERAVGLYAS IVNINAYHQP GVEAGKKAAA EVLALQKRVL SVLNEATCKD 550
    PVEPLTLEEI ADRCHAPEEI EMIYKIIAHM SANDRVLIAE GNCGSPRSIK 600
    VYLGECNVDD LYA 613
    Length:613
    Mass (Da):67,048
    Last modified:March 1, 2003 - v1
    Checksum:i95792BA6A173991B
    GO
    Isoform 2 (identifier: Q8H103-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         570-570: I → K
         571-613: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:570
    Mass (Da):62,308
    Checksum:i8B90C7BF2FBC24DA
    GO

    Sequence cautioni

    The sequence CAA23001.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB79372.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 4522ALPAQ…PTNLP → GIAGAISRFLLFPTYLQTHQ ST in AAF24124. (PubMed:10806248)CuratedAdd
    BLAST
    Sequence conflicti201 – 2077FVAEALA → LSLRLG in AAF24124. (PubMed:10806248)Curated
    Sequence conflicti321 – 3211A → H in AAF24124. (PubMed:10806248)Curated
    Sequence conflicti422 – 4221A → P in AAF24124. (PubMed:10806248)Curated
    Sequence conflicti444 – 4441D → Y in AAF24124. (PubMed:10806248)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei570 – 5701I → K in isoform 2. CuratedVSP_044431
    Alternative sequencei571 – 61343Missing in isoform 2. CuratedVSP_044432Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF120494 Genomic DNA. Translation: AAF24124.1.
    AL035356 Genomic DNA. Translation: CAA23001.1. Sequence problems.
    AL161561 Genomic DNA. Translation: CAB79372.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84933.1.
    CP002687 Genomic DNA. Translation: AEE84934.1.
    BT000953 mRNA. Translation: AAN41353.1.
    AK227111 mRNA. Translation: BAE99162.1.
    PIRiT05572.
    RefSeqiNP_001078444.1. NM_001084975.1. [Q8H103-2]
    NP_194193.2. NM_118595.4. [Q8H103-1]
    UniGeneiAt.25158.

    Genome annotation databases

    EnsemblPlantsiAT4G24620.1; AT4G24620.1; AT4G24620. [Q8H103-1]
    GeneIDi828564.
    KEGGiath:AT4G24620.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF120494 Genomic DNA. Translation: AAF24124.1 .
    AL035356 Genomic DNA. Translation: CAA23001.1 . Sequence problems.
    AL161561 Genomic DNA. Translation: CAB79372.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84933.1 .
    CP002687 Genomic DNA. Translation: AEE84934.1 .
    BT000953 mRNA. Translation: AAN41353.1 .
    AK227111 mRNA. Translation: BAE99162.1 .
    PIRi T05572.
    RefSeqi NP_001078444.1. NM_001084975.1. [Q8H103-2 ]
    NP_194193.2. NM_118595.4. [Q8H103-1 ]
    UniGenei At.25158.

    3D structure databases

    ProteinModelPortali Q8H103.
    SMRi Q8H103. Positions 83-545.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G24620.1-P.

    Proteomic databases

    PRIDEi Q8H103.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G24620.1 ; AT4G24620.1 ; AT4G24620 . [Q8H103-1 ]
    GeneIDi 828564.
    KEGGi ath:AT4G24620.

    Organism-specific databases

    TAIRi AT4G24620.

    Phylogenomic databases

    eggNOGi COG0166.
    HOGENOMi HOG000131425.
    InParanoidi Q8H103.
    KOi K01810.
    OMAi FTDVLWI.
    PhylomeDBi Q8H103.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00181 .
    UPA00138 .
    BioCyci MetaCyc:AT4G24620-MONOMER.

    Gene expression databases

    Genevestigatori Q8H103.

    Family and domain databases

    HAMAPi MF_00473. G6P_isomerase.
    InterProi IPR001672. G6P_Isomerase.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view ]
    PANTHERi PTHR11469. PTHR11469. 1 hit.
    Pfami PF00342. PGI. 2 hits.
    [Graphical view ]
    PRINTSi PR00662. G6PISOMERASE.
    PROSITEi PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mutation of Arabidopsis plastid phosphoglucose isomerase affects leaf starch synthesis and floral initiation."
      Yu T.-S., Lue W.-L., Wang S.-M., Chen J.
      Plant Physiol. 123:319-326(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF SER-166.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
      Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
      Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: MASS SPECTROMETRY [LARGE SCALE ANALYSIS], PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Root.
    9. "SUGAR-DEPENDENT6 encodes a mitochondrial flavin adenine dinucleotide-dependent glycerol-3-p dehydrogenase, which is required for glycerol catabolism and post germinative seedling growth in Arabidopsis."
      Quettier A.-L., Shaw E., Eastmond P.J.
      Plant Physiol. 148:519-528(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    10. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
      Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
      Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    13. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
      Olinares P.D., Ponnala L., van Wijk K.J.
      Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiG6PIP_ARATH
    AccessioniPrimary (citable) accession number: Q8H103
    Secondary accession number(s): A8MR16, Q9SB57, Q9SEJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3