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Q8H103

- G6PIP_ARATH

UniProt

Q8H103 - G6PIP_ARATH

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Protein

Glucose-6-phosphate isomerase 1, chloroplastic

Gene
PGI1, pgi2, pgi3, At4g24620, F22K18.180
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Promotes the synthesis of starch in leaves.1 Publication

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.UniRule annotation

Enzyme regulationi

Inhibited by glycerol-3-P (G3P).1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei392 – 3921Proton donor By similarity
Active sitei421 – 4211 By similarity
Active sitei526 – 5261 By similarity

GO - Molecular functioni

  1. glucose-6-phosphate isomerase activity Source: TAIR

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
  2. glycolytic process Source: UniProtKB-UniPathway
  3. positive regulation of flower development Source: TAIR
  4. starch metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciMetaCyc:AT4G24620-MONOMER.
UniPathwayiUPA00109; UER00181.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase 1, chloroplastic (EC:5.3.1.9)
Short name:
GPI 1
Alternative name(s):
Phosphoglucose isomerase 1
Short name:
PGI 1
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:PGI1
Synonyms:pgi2, pgi3
Ordered Locus Names:At4g24620
ORF Names:F22K18.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G24620.

Subcellular locationi

Plastidchloroplast stroma 5 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. cytosol Source: TAIR
  5. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi166 – 1661S → F in pgi1-1; decreased plastid phospho-glucose (Glc) isomerase activity leading to a deficiency in leaf starch synthesis, but an accumulation of starch in root cap cells. Delayed flowering time under short-day conditions. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848Chloroplast Reviewed predictionAdd
BLAST
Chaini49 – 613565Glucose-6-phosphate isomerase 1, chloroplasticUniRule annotationPRO_0000420249Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei595 – 5951Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ8H103.

Expressioni

Gene expression databases

GenevestigatoriQ8H103.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G24620.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8H103.
SMRiQ8H103. Positions 83-545.

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0166.
HOGENOMiHOG000131425.
InParanoidiQ8H103.
KOiK01810.
OMAiFTDVLWI.
PhylomeDBiQ8H103.

Family and domain databases

HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 2 hits.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8H103-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASLSGLYSS SPSLKPAKNH SFKALPAQSR DSFSFPHTSK PTNLPLTLSS    50
ARSVARDISH ADSKKELLKD PDALWKRYLD WFYQQKELGL YLDISRVGFT 100
DEFVAEMEPR FQAAFKAMED LEKGSIANPD EGRMVGHYWL RNSKLAPKPT 150
LKTLIENTLD SICAFSDDII SGKIKPPSSP EGRFTQILSV GIGGSALGPQ 200
FVAEALAPDN PPLKIRFIDN TDPAGIDHQI AQLGPELAST LVVVISKSGG 250
TPETRNGLLE VQKAFREAGL NFAKQGVAIT QENSLLDNTA RIEGWLARFP 300
MYDWVGGRTS IMSAVGLLPA ALQGINVREM LTGAALMDEA TRTTSIKNNP 350
AALLAMCWYW ASNGVGSKDM VVLPYKDSLL LFSRYLQQLV MESLGKEFDL 400
DGNTVNQGLT VYGNKGSTDQ HAYIQQLRDG VHNFFATFIE VLRDRPPGHD 450
WELEPGVTCG DYLFGMLQGT RSALYANGRE SISVTIQEVT PTSVGAIIAL 500
YERAVGLYAS IVNINAYHQP GVEAGKKAAA EVLALQKRVL SVLNEATCKD 550
PVEPLTLEEI ADRCHAPEEI EMIYKIIAHM SANDRVLIAE GNCGSPRSIK 600
VYLGECNVDD LYA 613
Length:613
Mass (Da):67,048
Last modified:March 1, 2003 - v1
Checksum:i95792BA6A173991B
GO
Isoform 2 (identifier: Q8H103-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     570-570: I → K
     571-613: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:570
Mass (Da):62,308
Checksum:i8B90C7BF2FBC24DA
GO

Sequence cautioni

The sequence CAA23001.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAB79372.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei570 – 5701I → K in isoform 2. VSP_044431
Alternative sequencei571 – 61343Missing in isoform 2. VSP_044432Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 4522ALPAQ…PTNLP → GIAGAISRFLLFPTYLQTHQ ST in AAF24124. 1 PublicationAdd
BLAST
Sequence conflicti201 – 2077FVAEALA → LSLRLG in AAF24124. 1 Publication
Sequence conflicti321 – 3211A → H in AAF24124. 1 Publication
Sequence conflicti422 – 4221A → P in AAF24124. 1 Publication
Sequence conflicti444 – 4441D → Y in AAF24124. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF120494 Genomic DNA. Translation: AAF24124.1.
AL035356 Genomic DNA. Translation: CAA23001.1. Sequence problems.
AL161561 Genomic DNA. Translation: CAB79372.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84933.1.
CP002687 Genomic DNA. Translation: AEE84934.1.
BT000953 mRNA. Translation: AAN41353.1.
AK227111 mRNA. Translation: BAE99162.1.
PIRiT05572.
RefSeqiNP_001078444.1. NM_001084975.1. [Q8H103-2]
NP_194193.2. NM_118595.4. [Q8H103-1]
UniGeneiAt.25158.

Genome annotation databases

EnsemblPlantsiAT4G24620.1; AT4G24620.1; AT4G24620. [Q8H103-1]
GeneIDi828564.
KEGGiath:AT4G24620.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF120494 Genomic DNA. Translation: AAF24124.1 .
AL035356 Genomic DNA. Translation: CAA23001.1 . Sequence problems.
AL161561 Genomic DNA. Translation: CAB79372.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE84933.1 .
CP002687 Genomic DNA. Translation: AEE84934.1 .
BT000953 mRNA. Translation: AAN41353.1 .
AK227111 mRNA. Translation: BAE99162.1 .
PIRi T05572.
RefSeqi NP_001078444.1. NM_001084975.1. [Q8H103-2 ]
NP_194193.2. NM_118595.4. [Q8H103-1 ]
UniGenei At.25158.

3D structure databases

ProteinModelPortali Q8H103.
SMRi Q8H103. Positions 83-545.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G24620.1-P.

Proteomic databases

PRIDEi Q8H103.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G24620.1 ; AT4G24620.1 ; AT4G24620 . [Q8H103-1 ]
GeneIDi 828564.
KEGGi ath:AT4G24620.

Organism-specific databases

TAIRi AT4G24620.

Phylogenomic databases

eggNOGi COG0166.
HOGENOMi HOG000131425.
InParanoidi Q8H103.
KOi K01810.
OMAi FTDVLWI.
PhylomeDBi Q8H103.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00181 .
UPA00138 .
BioCyci MetaCyc:AT4G24620-MONOMER.

Gene expression databases

Genevestigatori Q8H103.

Family and domain databases

HAMAPi MF_00473. G6P_isomerase.
InterProi IPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view ]
PANTHERi PTHR11469. PTHR11469. 1 hit.
Pfami PF00342. PGI. 2 hits.
[Graphical view ]
PRINTSi PR00662. G6PISOMERASE.
PROSITEi PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mutation of Arabidopsis plastid phosphoglucose isomerase affects leaf starch synthesis and floral initiation."
    Yu T.-S., Lue W.-L., Wang S.-M., Chen J.
    Plant Physiol. 123:319-326(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF SER-166.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
    Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
    Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: MASS SPECTROMETRY [LARGE SCALE ANALYSIS], PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  9. "SUGAR-DEPENDENT6 encodes a mitochondrial flavin adenine dinucleotide-dependent glycerol-3-p dehydrogenase, which is required for glycerol catabolism and post germinative seedling growth in Arabidopsis."
    Quettier A.-L., Shaw E., Eastmond P.J.
    Plant Physiol. 148:519-528(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  10. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiG6PIP_ARATH
AccessioniPrimary (citable) accession number: Q8H103
Secondary accession number(s): A8MR16, Q9SB57, Q9SEJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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