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Q8H103 (G6PIP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate isomerase 1, chloroplastic

Short name=GPI 1
EC=5.3.1.9
Alternative name(s):
Phosphoglucose isomerase 1
Short name=PGI 1
Phosphohexose isomerase
Short name=PHI
Gene names
Name:PGI1
Synonyms:pgi2, pgi3
Ordered Locus Names:At4g24620
ORF Names:F22K18.180
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length613 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes the synthesis of starch in leaves. Ref.1

Catalytic activity

D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP-Rule MF_00473

Enzyme regulation

Inhibited by glycerol-3-P (G3P). Ref.9

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP-Rule MF_00473

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00473

Subcellular location

Plastidchloroplast stroma Ref.6 Ref.7 Ref.10 Ref.12 Ref.13.

Sequence similarities

Belongs to the GPI family.

Sequence caution

The sequence CAA23001.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB79372.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8H103-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8H103-2)

The sequence of this isoform differs from the canonical sequence as follows:
     570-570: I → K
     571-613: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4848Chloroplast Potential
Chain49 – 613565Glucose-6-phosphate isomerase 1, chloroplastic HAMAP-Rule MF_00473
PRO_0000420249

Sites

Active site3921Proton donor By similarity
Active site4211 By similarity
Active site5261 By similarity

Amino acid modifications

Modified residue5951Phosphoserine Ref.8 Ref.11

Natural variations

Alternative sequence5701I → K in isoform 2.
VSP_044431
Alternative sequence571 – 61343Missing in isoform 2.
VSP_044432

Experimental info

Mutagenesis1661S → F in pgi1-1; decreased plastid phospho-glucose (Glc) isomerase activity leading to a deficiency in leaf starch synthesis, but an accumulation of starch in root cap cells. Delayed flowering time under short-day conditions. Ref.1
Sequence conflict24 – 4522ALPAQ…PTNLP → GIAGAISRFLLFPTYLQTHQ ST in AAF24124. Ref.1
Sequence conflict201 – 2077FVAEALA → LSLRLG in AAF24124. Ref.1
Sequence conflict3211A → H in AAF24124. Ref.1
Sequence conflict4221A → P in AAF24124. Ref.1
Sequence conflict4441D → Y in AAF24124. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 95792BA6A173991B

FASTA61367,048
        10         20         30         40         50         60 
MASLSGLYSS SPSLKPAKNH SFKALPAQSR DSFSFPHTSK PTNLPLTLSS ARSVARDISH 

        70         80         90        100        110        120 
ADSKKELLKD PDALWKRYLD WFYQQKELGL YLDISRVGFT DEFVAEMEPR FQAAFKAMED 

       130        140        150        160        170        180 
LEKGSIANPD EGRMVGHYWL RNSKLAPKPT LKTLIENTLD SICAFSDDII SGKIKPPSSP 

       190        200        210        220        230        240 
EGRFTQILSV GIGGSALGPQ FVAEALAPDN PPLKIRFIDN TDPAGIDHQI AQLGPELAST 

       250        260        270        280        290        300 
LVVVISKSGG TPETRNGLLE VQKAFREAGL NFAKQGVAIT QENSLLDNTA RIEGWLARFP 

       310        320        330        340        350        360 
MYDWVGGRTS IMSAVGLLPA ALQGINVREM LTGAALMDEA TRTTSIKNNP AALLAMCWYW 

       370        380        390        400        410        420 
ASNGVGSKDM VVLPYKDSLL LFSRYLQQLV MESLGKEFDL DGNTVNQGLT VYGNKGSTDQ 

       430        440        450        460        470        480 
HAYIQQLRDG VHNFFATFIE VLRDRPPGHD WELEPGVTCG DYLFGMLQGT RSALYANGRE 

       490        500        510        520        530        540 
SISVTIQEVT PTSVGAIIAL YERAVGLYAS IVNINAYHQP GVEAGKKAAA EVLALQKRVL 

       550        560        570        580        590        600 
SVLNEATCKD PVEPLTLEEI ADRCHAPEEI EMIYKIIAHM SANDRVLIAE GNCGSPRSIK 

       610 
VYLGECNVDD LYA 

« Hide

Isoform 2 [UniParc].

Checksum: 8B90C7BF2FBC24DA
Show »

FASTA57062,308

References

« Hide 'large scale' references
[1]"Mutation of Arabidopsis plastid phosphoglucose isomerase affects leaf starch synthesis and floral initiation."
Yu T.-S., Lue W.-L., Wang S.-M., Chen J.
Plant Physiol. 123:319-326(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF SER-166.
[2]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts."
Peltier J.-B., Cai Y., Sun Q., Zabrouskov V., Giacomelli L., Rudella A., Ytterberg A.J., Rutschow H., van Wijk K.J.
Mol. Cell. Proteomics 5:114-133(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis."
de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.
J. Proteome Res. 7:2458-2470(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY [LARGE SCALE ANALYSIS], PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Root.
[9]"SUGAR-DEPENDENT6 encodes a mitochondrial flavin adenine dinucleotide-dependent glycerol-3-p dehydrogenase, which is required for glycerol catabolism and post germinative seedling growth in Arabidopsis."
Quettier A.-L., Shaw E., Eastmond P.J.
Plant Physiol. 148:519-528(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[10]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[13]"Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
Olinares P.D., Ponnala L., van Wijk K.J.
Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF120494 Genomic DNA. Translation: AAF24124.1.
AL035356 Genomic DNA. Translation: CAA23001.1. Sequence problems.
AL161561 Genomic DNA. Translation: CAB79372.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84933.1.
CP002687 Genomic DNA. Translation: AEE84934.1.
BT000953 mRNA. Translation: AAN41353.1.
AK227111 mRNA. Translation: BAE99162.1.
PIRT05572.
RefSeqNP_001078444.1. NM_001084975.1.
NP_194193.2. NM_118595.4.
UniGeneAt.25158.

3D structure databases

ProteinModelPortalQ8H103.
SMRQ8H103. Positions 42-545.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT4G24620.1-P.

Proteomic databases

PRIDEQ8H103.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G24620.1; AT4G24620.1; AT4G24620. [Q8H103-1]
GeneID828564.
KEGGath:AT4G24620.

Organism-specific databases

TAIRAT4G24620.

Phylogenomic databases

eggNOGCOG0166.
HOGENOMHOG000131425.
InParanoidQ8H103.
KOK01810.
OMARQVGHYW.
PhylomeDBQ8H103.
ProtClustDBPLN02649.

Enzyme and pathway databases

BioCycMetaCyc:AT4G24620-MONOMER.
UniPathwayUPA00109; UER00181.
UPA00138.

Gene expression databases

GenevestigatorQ8H103.

Family and domain databases

HAMAPMF_00473. G6P_isomerase.
InterProIPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERPTHR11469. PTHR11469. 1 hit.
PfamPF00342. PGI. 2 hits.
[Graphical view]
PRINTSPR00662. G6PISOMERASE.
PROSITEPS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG6PIP_ARATH
AccessionPrimary (citable) accession number: Q8H103
Secondary accession number(s): A8MR16, Q9SB57, Q9SEJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names