Q8H103 (G6PIP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-6-phosphate isomerase 1, chloroplastic
Short name=GPI 1
EC=5.3.1.9

Alternative name(s):
Phosphoglucose isomerase 1
Short name=PGI 1
Phosphohexose isomerase
Short name=PHI

Gene names

Name:	PGI1
Synonyms:	pgi2, pgi3
Ordered Locus Names:	At4g24620
ORF Names:	F22K18.180

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes the synthesis of starch in leaves. Ref.1
Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP-Rule MF_00473
Enzyme regulation	Inhibited by glycerol-3-P (G3P). Ref.9
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP-Rule MF_00473 Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00473
Subcellular location	Plastid › chloroplast stroma Ref.6 Ref.7 Ref.10 Ref.12 Ref.13.
Sequence similarities	Belongs to the GPI family.
Sequence caution	The sequence CAA23001.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79372.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-UniPathway glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway positive regulation of flower development Inferred from mutant phenotype Ref.1. Source: TAIR starch metabolic process Inferred from mutant phenotype Ref.1. Source: TAIR
Cellular_component	chloroplast envelope Inferred from direct assay PubMed 12938931. Source: TAIR chloroplast stroma Inferred from direct assay Ref.7Ref.12. Source: TAIR cytosol Inferred from direct assay Ref.8. Source: TAIR
Molecular_function	glucose-6-phosphate isomerase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8H103-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8H103-2)

The sequence of this isoform differs from the canonical sequence as follows:
     570-570: I → K
     571-613: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 48	48	Chloroplast Potential
	Chain	49 – 613	565	Glucose-6-phosphate isomerase 1, chloroplastic HAMAP-Rule MF_00473		PRO_0000420249
Sites
	Active site	392	1	Proton donor By similarity
	Active site	421	1	By similarity
	Active site	526	1	By similarity
Amino acid modifications
	Modified residue	595	1	Phosphoserine Ref.8 Ref.11
Natural variations
	Alternative sequence	570	1	I → K in isoform 2.		VSP_044431
	Alternative sequence	571 – 613	43	Missing in isoform 2.		VSP_044432
Experimental info
	Mutagenesis	166	1	S → F in pgi1-1; decreased plastid phospho-glucose (Glc) isomerase activity leading to a deficiency in leaf starch synthesis, but an accumulation of starch in root cap cells. Delayed flowering time under short-day conditions. Ref.1
	Sequence conflict	24 – 45	22	ALPAQ…PTNLP → GIAGAISRFLLFPTYLQTHQ ST in AAF24124. Ref.1
	Sequence conflict	201 – 207	7	FVAEALA → LSLRLG in AAF24124. Ref.1
	Sequence conflict	321	1	A → H in AAF24124. Ref.1
	Sequence conflict	422	1	A → P in AAF24124. Ref.1
	Sequence conflict	444	1	D → Y in AAF24124. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 95792BA6A173991B	FASTA	613	67,048
	10 20 30 40 50 60 MASLSGLYSS SPSLKPAKNH SFKALPAQSR DSFSFPHTSK PTNLPLTLSS ARSVARDISH 70 80 90 100 110 120 ADSKKELLKD PDALWKRYLD WFYQQKELGL YLDISRVGFT DEFVAEMEPR FQAAFKAMED 130 140 150 160 170 180 LEKGSIANPD EGRMVGHYWL RNSKLAPKPT LKTLIENTLD SICAFSDDII SGKIKPPSSP 190 200 210 220 230 240 EGRFTQILSV GIGGSALGPQ FVAEALAPDN PPLKIRFIDN TDPAGIDHQI AQLGPELAST 250 260 270 280 290 300 LVVVISKSGG TPETRNGLLE VQKAFREAGL NFAKQGVAIT QENSLLDNTA RIEGWLARFP 310 320 330 340 350 360 MYDWVGGRTS IMSAVGLLPA ALQGINVREM LTGAALMDEA TRTTSIKNNP AALLAMCWYW 370 380 390 400 410 420 ASNGVGSKDM VVLPYKDSLL LFSRYLQQLV MESLGKEFDL DGNTVNQGLT VYGNKGSTDQ 430 440 450 460 470 480 HAYIQQLRDG VHNFFATFIE VLRDRPPGHD WELEPGVTCG DYLFGMLQGT RSALYANGRE 490 500 510 520 530 540 SISVTIQEVT PTSVGAIIAL YERAVGLYAS IVNINAYHQP GVEAGKKAAA EVLALQKRVL 550 560 570 580 590 600 SVLNEATCKD PVEPLTLEEI ADRCHAPEEI EMIYKIIAHM SANDRVLIAE GNCGSPRSIK 610 VYLGECNVDD LYA
	Isoform 2 [UniParc]. Checksum: 8B90C7BF2FBC24DA	FASTA	570	62,308
	10 20 30 40 50 60 MASLSGLYSS SPSLKPAKNH SFKALPAQSR DSFSFPHTSK PTNLPLTLSS ARSVARDISH 70 80 90 100 110 120 ADSKKELLKD PDALWKRYLD WFYQQKELGL YLDISRVGFT DEFVAEMEPR FQAAFKAMED 130 140 150 160 170 180 LEKGSIANPD EGRMVGHYWL RNSKLAPKPT LKTLIENTLD SICAFSDDII SGKIKPPSSP 190 200 210 220 230 240 EGRFTQILSV GIGGSALGPQ FVAEALAPDN PPLKIRFIDN TDPAGIDHQI AQLGPELAST 250 260 270 280 290 300 LVVVISKSGG TPETRNGLLE VQKAFREAGL NFAKQGVAIT QENSLLDNTA RIEGWLARFP 310 320 330 340 350 360 MYDWVGGRTS IMSAVGLLPA ALQGINVREM LTGAALMDEA TRTTSIKNNP AALLAMCWYW 370 380 390 400 410 420 ASNGVGSKDM VVLPYKDSLL LFSRYLQQLV MESLGKEFDL DGNTVNQGLT VYGNKGSTDQ 430 440 450 460 470 480 HAYIQQLRDG VHNFFATFIE VLRDRPPGHD WELEPGVTCG DYLFGMLQGT RSALYANGRE 490 500 510 520 530 540 SISVTIQEVT PTSVGAIIAL YERAVGLYAS IVNINAYHQP GVEAGKKAAA EVLALQKRVL 550 560 570 SVLNEATCKD PVEPLTLEEI ADRCHAPEEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutation of Arabidopsis plastid phosphoglucose isomerase affects leaf starch synthesis and floral initiation." Yu T.-S., Lue W.-L., Wang S.-M., Chen J. Plant Physiol. 123:319-326(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF SER-166.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions." Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S. Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts." Peltier J.-B., Cai Y., Sun Q., Zabrouskov V., Giacomelli L., Rudella A., Ytterberg A.J., Rutschow H., van Wijk K.J. Mol. Cell. Proteomics 5:114-133(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis." de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H. J. Proteome Res. 7:2458-2470(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Root.
[9]	"SUGAR-DEPENDENT6 encodes a mitochondrial flavin adenine dinucleotide-dependent glycerol-3-p dehydrogenase, which is required for glycerol catabolism and post germinative seedling growth in Arabidopsis." Quettier A.-L., Shaw E., Eastmond P.J. Plant Physiol. 148:519-528(2008) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION.
[10]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
[12]	"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins." Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N. Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering." Olinares P.D., Ponnala L., van Wijk K.J. Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF120494 Genomic DNA. Translation: AAF24124.1. AL035356 Genomic DNA. Translation: CAA23001.1. Sequence problems. AL161561 Genomic DNA. Translation: CAB79372.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE84933.1. CP002687 Genomic DNA. Translation: AEE84934.1. BT000953 mRNA. Translation: AAN41353.1. AK227111 mRNA. Translation: BAE99162.1.
IPI	IPI00544886. IPI00846783.
PIR	T05572.
RefSeq	NP_001078444.1. NM_001084975.1. NP_194193.2. NM_118595.4.
UniGene	At.25158.
3D structure databases
HSSP	HSSP built from PDB template 1C7R based on UniProtKB P13376.
ProteinModelPortal	Q8H103.
SMR	Q8H103. Positions 83-545.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT4G24620.1-P.
Proteomic databases
PRIDE	Q8H103.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G24620.1; AT4G24620.1; AT4G24620.
GeneID	828564.
KEGG	ath:AT4G24620.
Organism-specific databases
TAIR	At4g24620.
Phylogenomic databases
eggNOG	COG0166.
HOGENOM	HOG000131425.
InParanoid	Q8H103.
KO	K01810.
OMA	DGFLQGT.
PhylomeDB	Q8H103.
ProtClustDB	PLN02649.
Enzyme and pathway databases
BioCyc	ARA:AT4G24620-MONOMER. MetaCyc:AT4G24620-MONOMER.
UniPathway	UPA00109; UER00181. UPA00138.
Gene expression databases
ArrayExpress	Q8H103.
Genevestigator	Q8H103.
Family and domain databases
HAMAP	MF_00473. G6P_isomerase.
InterPro	IPR001672. G6P_Isomerase. IPR018189. Phosphoglucose_isomerase_CS. [Graphical view]
PANTHER	PTHR11469. PTHR11469. 1 hit.
Pfam	PF00342. PGI. 2 hits. [Graphical view]
PRINTS	PR00662. G6PISOMERASE.
PROSITE	PS00765. P_GLUCOSE_ISOMERASE_1. False negative. PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit. PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PIP_ARATH

Accession

Primary (citable) accession number: Q8H103
Secondary accession number(s): A8MR16, Q9SB57, Q9SEJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2012
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program