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Protein

Glucose-6-phosphate isomerase 1, chloroplastic

Gene

PGI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the synthesis of starch in leaves.1 Publication

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Enzyme regulationi

Inhibited by glycerol-3-P (G3P).1 Publication

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase 6, cytosolic (FAB6), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei392Proton donorBy similarity1
Active sitei421By similarity1
Active sitei526By similarity1

GO - Molecular functioni

  • glucose-6-phosphate isomerase activity Source: TAIR

GO - Biological processi

  • gluconeogenesis Source: UniProtKB-UniPathway
  • glycolytic process Source: UniProtKB-UniPathway
  • positive regulation of flower development Source: TAIR
  • starch metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciMetaCyc:AT4G24620-MONOMER.
UniPathwayiUPA00109; UER00181.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase 1, chloroplastic (EC:5.3.1.9)
Short name:
GPI 1
Alternative name(s):
Phosphoglucose isomerase 1
Short name:
PGI 1
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:PGI1
Synonyms:pgi2, pgi3
Ordered Locus Names:At4g24620
ORF Names:F22K18.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G24620.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166S → F in pgi1-1; decreased plastid phospho-glucose (Glc) isomerase activity leading to a deficiency in leaf starch synthesis, but an accumulation of starch in root cap cells. Delayed flowering time under short-day conditions. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48ChloroplastSequence analysisAdd BLAST48
ChainiPRO_000042024949 – 613Glucose-6-phosphate isomerase 1, chloroplasticAdd BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei595PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8H103.
PRIDEiQ8H103.

PTM databases

iPTMnetiQ8H103.

Expressioni

Gene expression databases

GenevisibleiQ8H103. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G24620.1.

Structurei

3D structure databases

ProteinModelPortaliQ8H103.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2446. Eukaryota.
COG0166. LUCA.
HOGENOMiHOG000131425.
InParanoidiQ8H103.
KOiK01810.
OMAiGEKDMVV.
OrthoDBiEOG093606IY.
PhylomeDBiQ8H103.

Family and domain databases

HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 2 hits.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8H103-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLSGLYSS SPSLKPAKNH SFKALPAQSR DSFSFPHTSK PTNLPLTLSS
60 70 80 90 100
ARSVARDISH ADSKKELLKD PDALWKRYLD WFYQQKELGL YLDISRVGFT
110 120 130 140 150
DEFVAEMEPR FQAAFKAMED LEKGSIANPD EGRMVGHYWL RNSKLAPKPT
160 170 180 190 200
LKTLIENTLD SICAFSDDII SGKIKPPSSP EGRFTQILSV GIGGSALGPQ
210 220 230 240 250
FVAEALAPDN PPLKIRFIDN TDPAGIDHQI AQLGPELAST LVVVISKSGG
260 270 280 290 300
TPETRNGLLE VQKAFREAGL NFAKQGVAIT QENSLLDNTA RIEGWLARFP
310 320 330 340 350
MYDWVGGRTS IMSAVGLLPA ALQGINVREM LTGAALMDEA TRTTSIKNNP
360 370 380 390 400
AALLAMCWYW ASNGVGSKDM VVLPYKDSLL LFSRYLQQLV MESLGKEFDL
410 420 430 440 450
DGNTVNQGLT VYGNKGSTDQ HAYIQQLRDG VHNFFATFIE VLRDRPPGHD
460 470 480 490 500
WELEPGVTCG DYLFGMLQGT RSALYANGRE SISVTIQEVT PTSVGAIIAL
510 520 530 540 550
YERAVGLYAS IVNINAYHQP GVEAGKKAAA EVLALQKRVL SVLNEATCKD
560 570 580 590 600
PVEPLTLEEI ADRCHAPEEI EMIYKIIAHM SANDRVLIAE GNCGSPRSIK
610
VYLGECNVDD LYA
Length:613
Mass (Da):67,048
Last modified:March 1, 2003 - v1
Checksum:i95792BA6A173991B
GO
Isoform 2 (identifier: Q8H103-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     570-570: I → K
     571-613: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:570
Mass (Da):62,308
Checksum:i8B90C7BF2FBC24DA
GO

Sequence cautioni

The sequence CAA23001 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79372 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24 – 45ALPAQ…PTNLP → GIAGAISRFLLFPTYLQTHQ ST in AAF24124 (PubMed:10806248).CuratedAdd BLAST22
Sequence conflicti201 – 207FVAEALA → LSLRLG in AAF24124 (PubMed:10806248).Curated7
Sequence conflicti321A → H in AAF24124 (PubMed:10806248).Curated1
Sequence conflicti422A → P in AAF24124 (PubMed:10806248).Curated1
Sequence conflicti444D → Y in AAF24124 (PubMed:10806248).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044431570I → K in isoform 2. Curated1
Alternative sequenceiVSP_044432571 – 613Missing in isoform 2. CuratedAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF120494 Genomic DNA. Translation: AAF24124.1.
AL035356 Genomic DNA. Translation: CAA23001.1. Sequence problems.
AL161561 Genomic DNA. Translation: CAB79372.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84933.1.
CP002687 Genomic DNA. Translation: AEE84934.1.
BT000953 mRNA. Translation: AAN41353.1.
AK227111 mRNA. Translation: BAE99162.1.
PIRiT05572.
RefSeqiNP_001078444.1. NM_001084975.1. [Q8H103-2]
NP_194193.2. NM_118595.5. [Q8H103-1]
UniGeneiAt.25158.

Genome annotation databases

EnsemblPlantsiAT4G24620.1; AT4G24620.1; AT4G24620. [Q8H103-1]
GeneIDi828564.
GrameneiAT4G24620.1; AT4G24620.1; AT4G24620.
KEGGiath:AT4G24620.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF120494 Genomic DNA. Translation: AAF24124.1.
AL035356 Genomic DNA. Translation: CAA23001.1. Sequence problems.
AL161561 Genomic DNA. Translation: CAB79372.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84933.1.
CP002687 Genomic DNA. Translation: AEE84934.1.
BT000953 mRNA. Translation: AAN41353.1.
AK227111 mRNA. Translation: BAE99162.1.
PIRiT05572.
RefSeqiNP_001078444.1. NM_001084975.1. [Q8H103-2]
NP_194193.2. NM_118595.5. [Q8H103-1]
UniGeneiAt.25158.

3D structure databases

ProteinModelPortaliQ8H103.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G24620.1.

PTM databases

iPTMnetiQ8H103.

Proteomic databases

PaxDbiQ8H103.
PRIDEiQ8H103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G24620.1; AT4G24620.1; AT4G24620. [Q8H103-1]
GeneIDi828564.
GrameneiAT4G24620.1; AT4G24620.1; AT4G24620.
KEGGiath:AT4G24620.

Organism-specific databases

TAIRiAT4G24620.

Phylogenomic databases

eggNOGiKOG2446. Eukaryota.
COG0166. LUCA.
HOGENOMiHOG000131425.
InParanoidiQ8H103.
KOiK01810.
OMAiGEKDMVV.
OrthoDBiEOG093606IY.
PhylomeDBiQ8H103.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
UPA00138.
BioCyciMetaCyc:AT4G24620-MONOMER.

Miscellaneous databases

PROiQ8H103.

Gene expression databases

GenevisibleiQ8H103. AT.

Family and domain databases

HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 2 hits.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PIP_ARATH
AccessioniPrimary (citable) accession number: Q8H103
Secondary accession number(s): A8MR16, Q9SB57, Q9SEJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.