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Protein

Cysteine proteinase inhibitor 6

Gene

CYS6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific inhibitor of cysteine proteinases. Probably involved in the regulation of endogenous processes and in defense against pests and pathogens (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei38Reactive siteBy similarity1

GO - Molecular functioni

  • cobalt ion binding Source: TAIR
  • cysteine-type endopeptidase inhibitor activity Source: TAIR
  • protease binding Source: GO_Central

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • hyperosmotic response Source: TAIR
  • negative regulation of cysteine-type endopeptidase activity Source: GO_Central
  • response to cold Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Keywords - Biological processi

Plant defense

Protein family/group databases

MEROPSiI25.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine proteinase inhibitor 6
Short name:
AtCYS-6
Alternative name(s):
PIP-M
PRLI-interacting factor M
Gene namesi
Name:CYS6
Ordered Locus Names:At3g12490
ORF Names:MQC3.31, T2E22.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G12490.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000027749825 – 234Cysteine proteinase inhibitor 6Add BLAST210
Isoform 2 (identifier: Q8H0X6-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei174PhosphoserineCombined sources1
Isoform 2 (identifier: Q8H0X6-2)
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8H0X6.
PRIDEiQ8H0X6.

PTM databases

iPTMnetiQ8H0X6.
SwissPalmiQ8H0X6.

Expressioni

Gene expression databases

GenevisibleiQ8H0X6. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CAN1F4IZC53EBI-8760191,EBI-8760221

GO - Molecular functioni

Protein-protein interaction databases

BioGridi5762. 2 interactors.
IntActiQ8H0X6. 1 interactor.
STRINGi3702.AT3G12490.2.

Structurei

3D structure databases

ProteinModelPortaliQ8H0X6.
SMRiQ8H0X6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 126Cystatin 1Add BLAST89
Domaini145 – 215Cystatin 2Add BLAST71

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi82 – 86Secondary area of contactBy similarity5

Sequence similaritiesi

Contains 2 cystatin domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IKPV. Eukaryota.
ENOG410YC0U. LUCA.
HOGENOMiHOG000006085.
InParanoidiQ8H0X6.
OMAiFKAEVHK.
OrthoDBiEOG09360MT5.
PhylomeDBiQ8H0X6.

Family and domain databases

CDDicd00042. CY. 2 hits.
InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 2 hits.
PfamiPF16845. SQAPI. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8H0X6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMRSRFLLFI VFFSLSLFIS SLIASDLGFC NEEMALVGGV GDVPANQNSG
60 70 80 90 100
EVESLARFAV DEHNKKENAL LEFARVVKAK EQVVAGTLHH LTLEILEAGQ
110 120 130 140 150
KKLYEAKVWV KPWLNFKELQ EFKPASDAPA ITSSDLGCKQ GEHESGWREV
160 170 180 190 200
PGDDPEVKHV AEQAVKTIQQ RSNSLFPYEL LEVVHAKAEV TGEAAKYNML
210 220 230
LKLKRGEKEE KFKVEVHKNH EGALHLNHAE QHHD
Length:234
Mass (Da):26,294
Last modified:February 6, 2007 - v2
Checksum:i233170F22A899F5F
GO
Isoform 2 (identifier: Q8H0X6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:201
Mass (Da):22,477
Checksum:i19F2E6BC9A7A2545
GO

Sequence cautioni

The sequence AAL38303 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123K → T in AAG31653 (PubMed:9765207).Curated1
Sequence conflicti160V → A in AAN65082 (PubMed:14593172).Curated1
Sequence conflicti226L → V in AAG31653 (PubMed:9765207).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0230221 – 33Missing in isoform 2. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP002047 Genomic DNA. Translation: BAB03156.1.
AC069474 Genomic DNA. Translation: AAG51028.1.
CP002686 Genomic DNA. Translation: AEE75201.1.
CP002686 Genomic DNA. Translation: AEE75202.1.
AY085950 mRNA. Translation: AAM63160.1.
AY065127 mRNA. Translation: AAL38303.1. Different initiation.
BT001195 mRNA. Translation: AAN65082.1.
AF315737 mRNA. Translation: AAG31653.1.
RefSeqiNP_850570.2. NM_180239.4. [Q8H0X6-1]
UniGeneiAt.16910.
At.48650.

Genome annotation databases

EnsemblPlantsiAT3G12490.2; AT3G12490.2; AT3G12490. [Q8H0X6-1]
GeneIDi820428.
GrameneiAT3G12490.2; AT3G12490.2; AT3G12490.
KEGGiath:AT3G12490.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP002047 Genomic DNA. Translation: BAB03156.1.
AC069474 Genomic DNA. Translation: AAG51028.1.
CP002686 Genomic DNA. Translation: AEE75201.1.
CP002686 Genomic DNA. Translation: AEE75202.1.
AY085950 mRNA. Translation: AAM63160.1.
AY065127 mRNA. Translation: AAL38303.1. Different initiation.
BT001195 mRNA. Translation: AAN65082.1.
AF315737 mRNA. Translation: AAG31653.1.
RefSeqiNP_850570.2. NM_180239.4. [Q8H0X6-1]
UniGeneiAt.16910.
At.48650.

3D structure databases

ProteinModelPortaliQ8H0X6.
SMRiQ8H0X6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5762. 2 interactors.
IntActiQ8H0X6. 1 interactor.
STRINGi3702.AT3G12490.2.

Protein family/group databases

MEROPSiI25.014.

PTM databases

iPTMnetiQ8H0X6.
SwissPalmiQ8H0X6.

Proteomic databases

PaxDbiQ8H0X6.
PRIDEiQ8H0X6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G12490.2; AT3G12490.2; AT3G12490. [Q8H0X6-1]
GeneIDi820428.
GrameneiAT3G12490.2; AT3G12490.2; AT3G12490.
KEGGiath:AT3G12490.

Organism-specific databases

TAIRiAT3G12490.

Phylogenomic databases

eggNOGiENOG410IKPV. Eukaryota.
ENOG410YC0U. LUCA.
HOGENOMiHOG000006085.
InParanoidiQ8H0X6.
OMAiFKAEVHK.
OrthoDBiEOG09360MT5.
PhylomeDBiQ8H0X6.

Miscellaneous databases

PROiQ8H0X6.

Gene expression databases

GenevisibleiQ8H0X6. AT.

Family and domain databases

CDDicd00042. CY. 2 hits.
InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 2 hits.
PfamiPF16845. SQAPI. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYT6_ARATH
AccessioniPrimary (citable) accession number: Q8H0X6
Secondary accession number(s): Q8VZA2, Q9FUB0, Q9LHF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: November 30, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.