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Protein

Cysteine proteinase inhibitor 6

Gene

CYS6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific inhibitor of cysteine proteinases. Probably involved in the regulation of endogenous processes and in defense against pests and pathogens (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei38 – 381Reactive siteBy similarity

GO - Molecular functioni

  • cobalt ion binding Source: TAIR
  • cysteine-type endopeptidase inhibitor activity Source: TAIR

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • hyperosmotic response Source: TAIR
  • negative regulation of endopeptidase activity Source: GOC
  • response to cold Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Keywords - Biological processi

Plant defense

Protein family/group databases

MEROPSiI25.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine proteinase inhibitor 6
Short name:
AtCYS-6
Alternative name(s):
PIP-M
PRLI-interacting factor M
Gene namesi
Name:CYS6
Ordered Locus Names:At3g12490
ORF Names:MQC3.31, T2E22.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G12490.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 234210Cysteine proteinase inhibitor 6PRO_0000277498Add
BLAST
Isoform 2 (identifier: Q8H0X6-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei174 – 1741PhosphoserineCombined sources
Isoform 2 (identifier: Q8H0X6-2)
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8H0X6.
PRIDEiQ8H0X6.

PTM databases

iPTMnetiQ8H0X6.
SwissPalmiQ8H0X6.

Expressioni

Gene expression databases

GenevisibleiQ8H0X6. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CAN1F4IZC53EBI-8760191,EBI-8760221

Protein-protein interaction databases

BioGridi5762. 2 interactions.
IntActiQ8H0X6. 1 interaction.
STRINGi3702.AT3G12490.2.

Structurei

3D structure databases

ProteinModelPortaliQ8H0X6.
SMRiQ8H0X6. Positions 32-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 12689Cystatin 1Add
BLAST
Domaini145 – 21571Cystatin 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi82 – 865Secondary area of contactBy similarity

Sequence similaritiesi

Contains 2 cystatin domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IKPV. Eukaryota.
ENOG410YC0U. LUCA.
HOGENOMiHOG000006085.
InParanoidiQ8H0X6.
OMAiFKAEVHK.
PhylomeDBiQ8H0X6.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR020381. Prot_inh_cystat_cons-reg.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 2 hits.
PfamiPF16845. SQAPI. 1 hit.
[Graphical view]
ProDomiPD001231. Prot_inh_cystat_cons-reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8H0X6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMRSRFLLFI VFFSLSLFIS SLIASDLGFC NEEMALVGGV GDVPANQNSG
60 70 80 90 100
EVESLARFAV DEHNKKENAL LEFARVVKAK EQVVAGTLHH LTLEILEAGQ
110 120 130 140 150
KKLYEAKVWV KPWLNFKELQ EFKPASDAPA ITSSDLGCKQ GEHESGWREV
160 170 180 190 200
PGDDPEVKHV AEQAVKTIQQ RSNSLFPYEL LEVVHAKAEV TGEAAKYNML
210 220 230
LKLKRGEKEE KFKVEVHKNH EGALHLNHAE QHHD
Length:234
Mass (Da):26,294
Last modified:February 6, 2007 - v2
Checksum:i233170F22A899F5F
GO
Isoform 2 (identifier: Q8H0X6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:201
Mass (Da):22,477
Checksum:i19F2E6BC9A7A2545
GO

Sequence cautioni

The sequence AAL38303.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti123 – 1231K → T in AAG31653 (PubMed:9765207).Curated
Sequence conflicti160 – 1601V → A in AAN65082 (PubMed:14593172).Curated
Sequence conflicti226 – 2261L → V in AAG31653 (PubMed:9765207).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3333Missing in isoform 2. 1 PublicationVSP_023022Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP002047 Genomic DNA. Translation: BAB03156.1.
AC069474 Genomic DNA. Translation: AAG51028.1.
CP002686 Genomic DNA. Translation: AEE75201.1.
CP002686 Genomic DNA. Translation: AEE75202.1.
AY085950 mRNA. Translation: AAM63160.1.
AY065127 mRNA. Translation: AAL38303.1. Different initiation.
BT001195 mRNA. Translation: AAN65082.1.
AF315737 mRNA. Translation: AAG31653.1.
RefSeqiNP_566425.1. NM_112084.3. [Q8H0X6-2]
NP_850570.2. NM_180239.3. [Q8H0X6-1]
UniGeneiAt.16910.
At.48650.

Genome annotation databases

EnsemblPlantsiAT3G12490.2; AT3G12490.2; AT3G12490. [Q8H0X6-1]
GeneIDi820428.
KEGGiath:AT3G12490.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP002047 Genomic DNA. Translation: BAB03156.1.
AC069474 Genomic DNA. Translation: AAG51028.1.
CP002686 Genomic DNA. Translation: AEE75201.1.
CP002686 Genomic DNA. Translation: AEE75202.1.
AY085950 mRNA. Translation: AAM63160.1.
AY065127 mRNA. Translation: AAL38303.1. Different initiation.
BT001195 mRNA. Translation: AAN65082.1.
AF315737 mRNA. Translation: AAG31653.1.
RefSeqiNP_566425.1. NM_112084.3. [Q8H0X6-2]
NP_850570.2. NM_180239.3. [Q8H0X6-1]
UniGeneiAt.16910.
At.48650.

3D structure databases

ProteinModelPortaliQ8H0X6.
SMRiQ8H0X6. Positions 32-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5762. 2 interactions.
IntActiQ8H0X6. 1 interaction.
STRINGi3702.AT3G12490.2.

Protein family/group databases

MEROPSiI25.014.

PTM databases

iPTMnetiQ8H0X6.
SwissPalmiQ8H0X6.

Proteomic databases

PaxDbiQ8H0X6.
PRIDEiQ8H0X6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G12490.2; AT3G12490.2; AT3G12490. [Q8H0X6-1]
GeneIDi820428.
KEGGiath:AT3G12490.

Organism-specific databases

TAIRiAT3G12490.

Phylogenomic databases

eggNOGiENOG410IKPV. Eukaryota.
ENOG410YC0U. LUCA.
HOGENOMiHOG000006085.
InParanoidiQ8H0X6.
OMAiFKAEVHK.
PhylomeDBiQ8H0X6.

Miscellaneous databases

PROiQ8H0X6.

Gene expression databases

GenevisibleiQ8H0X6. AT.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR020381. Prot_inh_cystat_cons-reg.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 2 hits.
PfamiPF16845. SQAPI. 1 hit.
[Graphical view]
ProDomiPD001231. Prot_inh_cystat_cons-reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-234 (ISOFORM 1).
    Strain: cv. Columbia.
  6. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-234 (ISOFORMS 1/2).
    Strain: cv. Columbia.
  7. "Comparative phylogenetic analysis of cystatin gene families from arabidopsis, rice and barley."
    Martinez M., Abraham Z., Carbonero P., Diaz I.
    Mol. Genet. Genomics 273:423-432(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  8. "Identification of phosphoproteins in Arabidopsis thaliana leaves using polyethylene glycol fractionation, immobilized metal-ion affinity chromatography, two-dimensional gel electrophoresis and mass spectrometry."
    Aryal U.K., Krochko J.E., Ross A.R.
    J. Proteome Res. 11:425-437(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-174, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2 (ISOFORM 2), CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS] (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCYT6_ARATH
AccessioniPrimary (citable) accession number: Q8H0X6
Secondary accession number(s): Q8VZA2, Q9FUB0, Q9LHF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: May 11, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.