Reviewed, UniProtKB/Swiss-Prot Q8H0W1 (PLSP1_ARATH)

Names and origin

Protein names

Recommended name:
    Chloroplast processing peptidase
    EC=3.4.21.89
Alternative name(s):
    Signal peptidase I-3

Gene names

Name:	PLSP1
Ordered Locus Names:	At3g24590
ORF Names:	MOB24.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the maturation of the plastid protein translocation channel. Required for the biogenesis of plastid internal membranes. May also function as a thylakoidal processing peptidase. Ref.4
Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Plastid › chloroplast envelope. Plastid › chloroplast thylakoid membrane. Note: May be located at the envelope membranes in premature chloroplast and at the thylakoidal membrane in mature chloroplasts. Ref.4
Disruption phenotype	Plants are albino and seedling lethal. Ref.4
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast envelope Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 67	67	Chloroplast Potential
	Chain	68 – 291	224	Chloroplast processing peptidase		PRO_0000310736
Regions
	Compositional bias	82 – 90	9	Poly-Gly
Sites
	Active site	142	1	By similarity
Experimental info
	Sequence conflict	209	1	L → Q in AAN72018. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8H0W1-1 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: FB353211D47FC737	FASTA	291	32,554
	10 20 30 40 50 60 MMVMISLHFS TPPLAFLKSD SNSRFLKNPN PNFIQFTPKS QLLFPQRLNF NTGTNLNRRT 70 80 90 100 110 120 LSCYGIKDSS ETTKSAPSLD SGDGGGGDGG DDDKGEVEEK NRLFPEWLDF TSDDAQTVFV 130 140 150 160 170 180 AIAVSLAFRY FIAEPRYIPS LSMYPTFDVG DRLVAEKVSY YFRKPCANDI VIFKSPPVLQ 190 200 210 220 230 240 EVGYTDADVF IKRIVAKEGD LVEVHNGKLM VNGVARNEKF ILEPPGYEMT PIRVPENSVF 250 260 270 280 290 VMGDNRNNSY DSHVWGPLPL KNIIGRSVFR YWPPNRVSGT VLEGGCAVDK Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Complete maturation of the plastid protein translocation channel requires a type I signal peptidase." Inoue K., Baldwin A.J., Shipman R.L., Matsui K., Theg S.M., Ohme-Takagi M. J. Cell Biol. 171:425-430(2005) [PubMed: 16275749] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB020746 Genomic DNA. Translation: BAB02011.1. Different initiation. BT002007 mRNA. Translation: AAN72018.1. BT015406 mRNA. Translation: AAU05529.1.
IPI	IPI00516564.
RefSeq	NP_189102.2.
UniGene	At.37497
3D structure databases
HSSP	HSSP built from PDB template 1B12 based on UniProtKB P00803.
ModBase	Search...
Protein family/group databases
MEROPS	S26.A02.
Proteomic databases
PRIDE	Q8H0W1.
Genome annotation databases
GeneID	822055.
GenomeReviews	Gene locus AT3G24590 in contig BA000014_GR.
KEGG	ath:AT3G24590.
NMPDR	fig|3702.1.peg.14727.
Organism-specific databases
GeneFarm	2139. 205.
TAIR	At3g24590.
Phylogenomic databases
OMA	Q8H0W1. QVPENSV.
Enzyme and pathway databases
BRENDA	3.4.21.89. 302.
Gene expression databases
ArrayExpress	Q8H0W1.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSP1_ARATH

Accession

Primary (citable) accession number: Q8H0W1
Secondary accession number(s): Q66GJ6, Q9LV44

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)