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Protein

E3 ubiquitin-protein ligase UPL2

Gene

UPL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3625Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase UPL2 (EC:2.3.2.26)
Short name:
Ubiquitin-protein ligase 2
Alternative name(s):
HECT-type E3 ubiquitin transferase UPL2
Gene namesi
Name:UPL2
Ordered Locus Names:At1g70320
ORF Names:F17O7.14/F17O7.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G70320.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • mitochondrion Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203441 – 3658E3 ubiquitin-protein ligase UPL2Add BLAST3658

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2582PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8H0T4.
PRIDEiQ8H0T4.

PTM databases

iPTMnetiQ8H0T4.

Expressioni

Tissue specificityi

Widely expressed. Expressed in root, stem, cauline and rosette leaf, seedling and flower (at protein level).1 Publication

Developmental stagei

Constitutively expressed throughout development post-germination (at protein level).1 Publication

Gene expression databases

GenevisibleiQ8H0T4. AT.

Interactioni

Protein-protein interaction databases

BioGridi28588. 5 interactors.
IntActiQ8H0T4. 1 interactor.
MINTiMINT-8063873.
STRINGi3702.AT1G70320.1.

Structurei

3D structure databases

ProteinModelPortaliQ8H0T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1271 – 1312UBAPROSITE-ProRule annotationAdd BLAST42
Domaini1318 – 1337UIMPROSITE-ProRule annotationAdd BLAST20
Domaini3317 – 3658HECTPROSITE-ProRule annotationAdd BLAST342

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2116 – 2210Asp-richAdd BLAST95

Sequence similaritiesi

Belongs to the UPL family. TOM1/PTR1 subfamily.Curated
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0939. Eukaryota.
COG5021. LUCA.
HOGENOMiHOG000084686.
InParanoidiQ8H0T4.
KOiK10592.
OMAiSSVIQWF.
OrthoDBiEOG0936000C.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR025527. DUF4414.
IPR010309. E3_Ub_ligase_DUF908.
IPR010314. E3_Ub_ligase_DUF913.
IPR000569. HECT_dom.
IPR015940. UBA.
IPR009060. UBA-like.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF14377. DUF4414. 1 hit.
PF06012. DUF908. 2 hits.
PF06025. DUF913. 1 hit.
PF00632. HECT. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF48371. SSF48371. 3 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
PS50030. UBA. 1 hit.
PS50330. UIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8H0T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRRRRASE VPTKISLFIN SVTSVPLELI QEPLASFRWE FDKGDFHHWV
60 70 80 90 100
DLFYHFDTFF EKHVKVRKDL RIEEEFDESD PPFPKDAVLQ VLRVIRLVLE
110 120 130 140 150
NCTNKQFYTS YEQHLSLLLA STDADVVEAC LQTLAAFLKR PTGKYSIRDA
160 170 180 190 200
SLNLKLFSLA QGWGGKEEGL GLTSCATEHS CDQLFLQLGC TLLFEFYASD
210 220 230 240 250
ESPSELPGGL QVIHVPDVSM RSESDLELLN KLVIDHNVPP SLRFALLTRL
260 270 280 290 300
RFARAFSSLA TRQQYTCIRL YAFIVLVQAS GDTENVVSFF NGEPEFVNEL
310 320 330 340 350
VTLVSYEDTV PAKIRILCLQ SLVALSQDRT RQPTVLTAVT SGGHRGLLSG
360 370 380 390 400
LMQKAIDSVI CNTSKWSLAF AEALLSLVTV LVSSSSGCSA MREAGLIPTL
410 420 430 440 450
VPLIKDTDPQ HLHLVSTAVH ILEVFMDYSN PAAALFRDLG GLDDTIFRLK
460 470 480 490 500
QEVSRTEDDV KEIVCCSGSN GPEDDTEQLP YSEALISYHR RLLLKALLRA
510 520 530 540 550
ISLGTYAPGN TNLYGSEESL LPECLCIIFR RAKDFGGGVF SLAATVMSDL
560 570 580 590 600
IHKDPTCFNA LDSAGLTSAF LDAISDEVIC SAEAITCIPQ CLDALCLNNS
610 620 630 640 650
GLQAVKDRNA LRCFVKIFSS PSYLKALTSD TPGSLSSGLD ELLRHQSSLR
660 670 680 690 700
TYGVDMFIEI LNSILIIGSG MEATTSKSAD VPTDAAPVPM EIDVDEKSLA
710 720 730 740 750
VSDEAEPSSD TSPANIELFL PDCVCNVARL FETVLQNAEV CSLFVEKKGI
760 770 780 790 800
DTVLQLFSLP LMPLSTSLGQ SFSVAFKNFS PQHSAGLARI LCSYLREHLK
810 820 830 840 850
KTNNLLVSIE GTQLLKLESA VQTKILRSLS CLEGMLSLSN FLLKGSASVI
860 870 880 890 900
SELSAANADV LKELGITYKQ TIWQMALCND TKEDEKKSVD RASDNSVSAS
910 920 930 940 950
SSTAERESDE DSSNALAVRY TNPVSIRSSS SQSIWGGHRE FLSVVRSGRG
960 970 980 990 1000
VHGHTRHAIA RMRGGRTRRH LESFNFDSEI PADLPVTSSS HELKKKSTEV
1010 1020 1030 1040 1050
LIAEILNKLN CTLRFFFTSL VKGFTSANRR RIDGPSLSSA SKTLGTALAK
1060 1070 1080 1090 1100
VFLEALNFQG YGAAAGPDTS LSLKCRYLGK VVDDITFLTF DTRRRVCFTA
1110 1120 1130 1140 1150
MVNSFYVHGT FKELLTTFEA TSQLLWKVPF SIRASSTENE KSGERNLWSH
1160 1170 1180 1190 1200
SKWLVDTLQN YCRALDYFVN STYLLSPTSQ TQLLVQPASV DLSIGLFPVP
1210 1220 1230 1240 1250
REPETFVRNL QSQVLEVILP IWNHPMFPDC NPNFVASVTS LVTHIYSGVV
1260 1270 1280 1290 1300
DTRENRSGAT QGTNQRALPL QPDEAIVGMI VEMGFSRSRA EDALRRVGTN
1310 1320 1330 1340 1350
SVEMAMDWLF TNPEDPVQED DELAQALALS LGNSSETPKL EDTEKPVDVP
1360 1370 1380 1390 1400
QEEAEPKEPP VDEVIAASVK LFQSDDSIAF PLVDLFVTLC NRNKGEDRPK
1410 1420 1430 1440 1450
IVFYLIQQLK LVQLDFSKDT GALTMIPHIL ALVLSEDDNT REIAAQDGIV
1460 1470 1480 1490 1500
AVAIGILTDF NLKSESETDI LAPKCISALL LVLSMMLQAQ TRLSSEYVEG
1510 1520 1530 1540 1550
NQGGSLVLSD SPQDSTAALK DALSSDVAKG ESNQALESMF GKSTGYLTME
1560 1570 1580 1590 1600
ESSKVLLIAC GLIKQRVPAM IMQAVLQLCA RLTKSHALAI QFLENGGLSS
1610 1620 1630 1640 1650
LFNLPKKCFF PGYDTVASVI VRHLVEDPQT LQIAMETEIR QTLSGKRHIG
1660 1670 1680 1690 1700
RVLPRTFLTT MAPVISRDPV VFMKAVASTC QLESSGGTDF VILTKEKEKP
1710 1720 1730 1740 1750
KVSGSEHGFS LNEPLGISEN KLHDGSGKCS KSHRRVPTNF IQVIDQLIDI
1760 1770 1780 1790 1800
VLSFPGLKRQ EGEAANLISM DVDEPTTKVK GKSKVGEPEK AELGSEKSEE
1810 1820 1830 1840 1850
LARVTFILKL LSDIVLMYLH GTSVILRRDT EISQLRGSNL PDDSPGNGGL
1860 1870 1880 1890 1900
IYHVIHRLLP ISLEKFVGPE EWKEKLSEKA SWFLVVLCSR SNEGRKRIIN
1910 1920 1930 1940 1950
ELTRVLSVFA SLGRSSSQSV LLPDKRVLAF ANLVYSILTK NSSSSNFPGC
1960 1970 1980 1990 2000
GCSPDVAKSM IDGGTIQCLT SILNVIDLDH PDAPKLVTLI LKSLETLTRA
2010 2020 2030 2040 2050
ANAAEQLKSE VPNEQKNTDS DERHDSHGTS TSTEVDELNQ NNSSLQQVTD
2060 2070 2080 2090 2100
AVDNGQEQPQ VSSQSEGERG SSLTQAMLQE MRIEGDETIL PEPIQMDFFR
2110 2120 2130 2140 2150
EEIEGDQIEM SFHVEDRADD DVDDDMDDEG EDDEGDDEDA DSVEDGAGVM
2160 2170 2180 2190 2200
SIAGTDVEDP EDTGLGDEYN DDMVDEDEED EDEYNDDMVD EDEDDEDEYN
2210 2220 2230 2240 2250
DDMVDEDEDD FHETRVIEVR WREALDGLDH FQIVGRSGGG NGFIDDITAE
2260 2270 2280 2290 2300
PFEGVNVDDL FALRRSLGFE RRRQTGRSSF DRSGSEVHGF QHPLFSRPSQ
2310 2320 2330 2340 2350
TGNTASVSAS AGSISRHSEA GSYDVAQFYM FDSPVLPFDQ VPVDPFSDRL
2360 2370 2380 2390 2400
GGGGAPPPLT DYSVVGMDSS RRGVGDSRWT DVGHPQPSSL SASIAQLIEE
2410 2420 2430 2440 2450
HFITNLRASA PVDTVVERET NTTEVQEQQQ PDVPPSVGSE TVLGDGNEGG
2460 2470 2480 2490 2500
EQSEEHELLN NNEVMHPLPL NSTPNEIDRM EVGEGGGAPI EQVDREAVHL
2510 2520 2530 2540 2550
ISSAQGQSDT SGIQNVSVTA IPPPVDDPDS NFQPSVDVDM SSDGAEGNQS
2560 2570 2580 2590 2600
VQPSPLDGDN NELSSMEATQ DVRNDEQVDE GSLDGRAPEV NAIDPTFLEA
2610 2620 2630 2640 2650
LPEDLRAEVL ASQQAQSVQP PTYEPPSVDD IDPEFLAALP PEIQREVLAQ
2660 2670 2680 2690 2700
QRAQRMLQQS QGQPVDMDNA SIIATLPADL REEVLLTSSE AVLAALPPPL
2710 2720 2730 2740 2750
LAEAQMLRDR AMRHYQARSR VFGSSHRLNN RRNGLGYRLT GMERGVGVTI
2760 2770 2780 2790 2800
GQRDVSSSAD GLKVKEIEGD PLVNADALKS LIRLLRLAQP LGKGLLQRLL
2810 2820 2830 2840 2850
LNLCAHSFTR ANLVQLLLDM IRPEMETLPS ELALTNPQRL YGCQLNVVYG
2860 2870 2880 2890 2900
RSQLLNGLPP LVFRRVLEVL TYLATNHSAV ADMLFYFDSS LLSQLSSRKG
2910 2920 2930 2940 2950
KEKVTHETDS RDLEIPLVVF LKLLNRPQLL QSTSHLALVM GLLQVVVYTA
2960 2970 2980 2990 3000
ASRIEGWSPS SGVPEKLENK PVGEEASSET QKDAESELSV ARRKNCAELY
3010 3020 3030 3040 3050
NIFLQLPQSD LCNLCMLLGY EGLSDKIYSL AGEVLKKLAA VDVTHRKFFT
3060 3070 3080 3090 3100
KELSELASGL SSSTVRVLAT LSTTQKMSQN TCSMAGASIL RVLQVLSSLT
3110 3120 3130 3140 3150
STIDDSNVGT DKETDQEEQN IMQGLKVALE PLWQELGQCI SMTELQLDHT
3160 3170 3180 3190 3200
AATSNVNPGD HVLGISPTSS LSPGTQSLLP LIEAFFVLCE KIQTPSMLQQ
3210 3220 3230 3240 3250
DATVTAGEVK ESSTHGSSSK TIVDSQKKID GSVTFSKFVE KHRRLLNSFV
3260 3270 3280 3290 3300
RQNPSLLEKS FSMMLKAPRL IDFDNKKAYF RSRIRHQHDQ HISGPLRISV
3310 3320 3330 3340 3350
RRAYVLEDSY NQLRMRSPQD LKGRLNVQFQ GEEGIDAGGL TREWYQLLSR
3360 3370 3380 3390 3400
VIFDKGALLF TTVGNDATFQ PNPNSVYQTE HLSYFKFVGR MVAKALFDGQ
3410 3420 3430 3440 3450
LLDVYFTRSF YKHILGVKVT YHDIEAVDPD YYKNLKWLLE NDVSDILDLT
3460 3470 3480 3490 3500
FSMDADEEKH ILYEKTEVTD YELKPGGRNI RVTEETKHEY VDLVADHILT
3510 3520 3530 3540 3550
SAIRPQINAF LEGLNELIPR ELVSIFNDKE LELLISGLPE IDFDDLKANT
3560 3570 3580 3590 3600
EYTSYTVGSP VIRWFWEVVK AFSKEDMARF LQFVTGTSKV PLEGFKALQG
3610 3620 3630 3640 3650
ISGPQRLQIH KAYGSPERLP SAHTCFNQLD LPEYQSKEQV QERLLLAIHE

ANEGFGFA
Length:3,658
Mass (Da):403,616
Last modified:May 3, 2011 - v3
Checksum:iC6D74894C0264444
GO

Sequence cautioni

The sequence AAC18812 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes.Curated
The sequence AAC18813 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes.Curated
The sequence AAN72076 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153 – 154NL → ES in AAF36455 (PubMed:10571878).Curated2
Sequence conflicti263Q → R in AAF36455 (PubMed:10571878).Curated1
Sequence conflicti1759R → S in AAF36455 (PubMed:10571878).Curated1
Sequence conflicti1771D → N in AAF36455 (PubMed:10571878).Curated1
Sequence conflicti2322S → I in AAF36455 (PubMed:10571878).Curated1
Sequence conflicti3298I → F in AAN72076 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127565 Genomic DNA. Translation: AAF36455.1.
AC003671 Genomic DNA. Translation: AAC18812.1. Sequence problems.
AC003671 Genomic DNA. Translation: AAC18813.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35045.1.
AK230373 mRNA. Translation: BAF02172.1.
BT002065 mRNA. Translation: AAN72076.1. Different initiation.
BT008830 mRNA. Translation: AAP68269.1.
PIRiT01490.
T01491.
RefSeqiNP_177189.1. NM_105700.3.
UniGeneiAt.46269.

Genome annotation databases

EnsemblPlantsiAT1G70320.1; AT1G70320.1; AT1G70320.
GeneIDi843368.
GrameneiAT1G70320.1; AT1G70320.1; AT1G70320.
KEGGiath:AT1G70320.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127565 Genomic DNA. Translation: AAF36455.1.
AC003671 Genomic DNA. Translation: AAC18812.1. Sequence problems.
AC003671 Genomic DNA. Translation: AAC18813.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35045.1.
AK230373 mRNA. Translation: BAF02172.1.
BT002065 mRNA. Translation: AAN72076.1. Different initiation.
BT008830 mRNA. Translation: AAP68269.1.
PIRiT01490.
T01491.
RefSeqiNP_177189.1. NM_105700.3.
UniGeneiAt.46269.

3D structure databases

ProteinModelPortaliQ8H0T4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28588. 5 interactors.
IntActiQ8H0T4. 1 interactor.
MINTiMINT-8063873.
STRINGi3702.AT1G70320.1.

PTM databases

iPTMnetiQ8H0T4.

Proteomic databases

PaxDbiQ8H0T4.
PRIDEiQ8H0T4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G70320.1; AT1G70320.1; AT1G70320.
GeneIDi843368.
GrameneiAT1G70320.1; AT1G70320.1; AT1G70320.
KEGGiath:AT1G70320.

Organism-specific databases

TAIRiAT1G70320.

Phylogenomic databases

eggNOGiKOG0939. Eukaryota.
COG5021. LUCA.
HOGENOMiHOG000084686.
InParanoidiQ8H0T4.
KOiK10592.
OMAiSSVIQWF.
OrthoDBiEOG0936000C.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ8H0T4.

Gene expression databases

GenevisibleiQ8H0T4. AT.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR025527. DUF4414.
IPR010309. E3_Ub_ligase_DUF908.
IPR010314. E3_Ub_ligase_DUF913.
IPR000569. HECT_dom.
IPR015940. UBA.
IPR009060. UBA-like.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF14377. DUF4414. 1 hit.
PF06012. DUF908. 2 hits.
PF06025. DUF913. 1 hit.
PF00632. HECT. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF48371. SSF48371. 3 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
PS50030. UBA. 1 hit.
PS50330. UIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUPL2_ARATH
AccessioniPrimary (citable) accession number: Q8H0T4
Secondary accession number(s): O64604
, O64605, Q0WL35, Q9M7K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 3, 2011
Last modified: November 30, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.