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Protein

Anthocyanin 3'-O-beta-glucosyltransferase

Gene
N/A
Organism
Gentiana triflora (Clustered gentian)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically glucosylates the 3'-hydroxy group of delphinidin 3,5-di-O-glucoside to produce gentiodelphin. Shows a strict specificity for UDP-glucose as donor.

Catalytic activityi

UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside.

Kineticsi

  1. KM=120 µM for delphinidin 3,5-di-O-glucoside1 Publication
  1. Vmax=1370 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.238. 2412.
SABIO-RKQ8H0F2.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthocyanin 3'-O-beta-glucosyltransferase (EC:2.4.1.238)
Short name:
3'GT
OrganismiGentiana triflora (Clustered gentian)
Taxonomic identifieri55190 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesGentianaceaeGentianeaeGentiana

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003107291 – 482Anthocyanin 3'-O-beta-glucosyltransferaseAdd BLAST482

Post-translational modificationi

The N-terminus is blocked.

Expressioni

Tissue specificityi

Abundant in petals and barely detected in leaves.1 Publication

Developmental stagei

Expressed early in petals development and decreases in fully opened flowers.1 Publication

Inductioni

Significantly inhibited by Al3+ and Co2+, moderately by Zn2+ and not by Mg2+, Ca2+, Mn2+, EDAT or EGTA. Competitively inhibited by UDP.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8H0F2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

KOiK12939.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8H0F2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQLHVFFFP FLANGHILPT IDMAKLFSSR GVKATLITTH NNSAIFLKAI
60 70 80 90 100
NRSKILGFDI SVLTIKFPSA EFGLPEGYET ADQARSIDMM DEFFRACILL
110 120 130 140 150
QEPLEELLKE HRPQALVADL FFYWANDAAA KFGIPRLLFH GSSSFAMIAA
160 170 180 190 200
ESVRRNKPYK NLSSDSDPFV VPDIPDKIIL TKSQVPTPDE TEENNTHITE
210 220 230 240 250
MWKNISESEN DCYGVIVNSF YELEPDYVDY CKNVLGRRAW HIGPLSLCNN
260 270 280 290 300
EGEDVAERGK KSDIDAHECL NWLDSKNPDS VVYVCFGSMA NFNAAQLHEL
310 320 330 340 350
AMGLEESGQE FIWVVRTCVD EEDESKWFPD GFEKRVQENN KGLIIKGWAP
360 370 380 390 400
QVLILEHEAV GAFVSHCGWN STLEGICGGV AMVTWPLFAE QFYNEKLMTD
410 420 430 440 450
ILRTGVSVGS LQWSRVTTSA VVVKRESISK AVRRLMAEEE GVDIRNRAKA
460 470 480
LKEKAKKAVE GGGSSYSDLS ALLVELSSYP HN
Length:482
Mass (Da):54,041
Last modified:March 1, 2003 - v1
Checksum:iBDBC12F21C7F7147
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB076697 mRNA. Translation: BAC54092.1.

Genome annotation databases

KEGGiag:BAC54092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB076697 mRNA. Translation: BAC54092.1.

3D structure databases

ProteinModelPortaliQ8H0F2.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAC54092.

Phylogenomic databases

KOiK12939.

Enzyme and pathway databases

BRENDAi2.4.1.238. 2412.
SABIO-RKQ8H0F2.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANGT_GENTR
AccessioniPrimary (citable) accession number: Q8H0F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.