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Protein

3,7-dimethylxanthine N-methyltransferase

Gene

DXMT1

Organism
Coffea arabica (Arabian coffee)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of caffeine. Catalyzes the conversion of paraxanthine to caffeine. Can also convert 7-methylxanthine to theobromine and theobromine to caffeine, but no sequential conversion of 7-methylxanthine to caffeine was detected.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + 3,7-dimethylxanthine = S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine.1 Publication
S-adenosyl-L-methionine + 1,7-dimethylxanthine = S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine.1 Publication
S-adenosyl-L-methionine + 7-methylxanthine = S-adenosyl-L-homocysteine + 3,7-dimethylxanthine.1 Publication

Kineticsi

  1. KM=916 µM for 7-methylxanthine1 Publication
  2. KM=973 µM for paraxanthine1 Publication
  3. KM=1222 µM for theobromine1 Publication
  4. KM=153 µM for S-adenosyl-L-methionine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei18 – 181S-adenosyl-L-methionineBy similarity
    Binding sitei18 – 181SubstrateBy similarity
    Binding sitei60 – 601S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei66 – 661S-adenosyl-L-methionineBy similarity
    Binding sitei237 – 2371SubstrateBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Alkaloid metabolism

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BRENDAi2.1.1.160. 1559.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3,7-dimethylxanthine N-methyltransferase (EC:2.1.1.160)
    Short name:
    CaDXMT1
    Alternative name(s):
    Caffeine synthase 7
    Short name:
    CtCS7
    Gene namesi
    Name:DXMT1
    Synonyms:CS7
    OrganismiCoffea arabica (Arabian coffee)
    Taxonomic identifieri13443 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesRubiaceaeIxoroideaeCoffeeaeCoffea

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3843843,7-dimethylxanthine N-methyltransferasePRO_0000408306Add
    BLAST

    Expressioni

    Tissue specificityi

    Highly expressed in developing endosperm and immature fruits. Detected in young leaves and flower buds, but not in mature fruits.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ8H0D2.
    SMRiQ8H0D2. Positions 3-379.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni99 – 1013S-adenosyl-L-methionine bindingBy similarity
    Regioni139 – 1402S-adenosyl-L-methionine bindingBy similarity
    Regioni156 – 1583S-adenosyl-L-methionine bindingBy similarity
    Regioni157 – 1615Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    KOiK12731.

    Family and domain databases

    Gene3Di3.40.50.150. 3 hits.
    InterProiIPR005299. MeTrfase_7.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PfamiPF03492. Methyltransf_7. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8H0D2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MELQEVLHMN GGEGDTSYAK NSFYNLFLIR VKPILEQCIQ ELLRANLPNI
    60 70 80 90 100
    NKCIKVADLG CASGPNTLLT VRDIVQSIDK VGQEKKNELE RPTIQIFLND
    110 120 130 140 150
    LFQNDFNSVF KSLPSFYRKL EKENGCKIGS CLIGAMPGSF YGRLFPEESM
    160 170 180 190 200
    HFLHSCYCLH WLSQVPSGLV TELGISANKG CIYSSKASRP PIQKAYLDQF
    210 220 230 240 250
    TKDFTTFLRI HSEELISRGR MLLTWICKED EFENPNSIDL LEMSINDLVI
    260 270 280 290 300
    EGHLEEEKLD SFNVPIYAPS TEEVKCIVEE EGSFEILYLE TFKVPYDAGF
    310 320 330 340 350
    SIDDDYQGRS HSPVSCDEHA RAAHVASVVR SIFEPIVASH FGEAILPDLS
    360 370 380
    HRIAKNAAKV LRSGKGFYDS VIISLAKKPE KADM
    Length:384
    Mass (Da):43,248
    Last modified:March 1, 2003 - v1
    Checksum:i50FF35F03F64133F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti126 – 1261C → R in BAC75663 (PubMed:12746542).Curated
    Sequence conflicti346 – 3461L → M in BAC75663 (PubMed:12746542).Curated
    Sequence conflicti371 – 3711V → L in BAC75663 (PubMed:12746542).Curated
    Sequence conflicti382 – 3843ADM → SDV in BAC75663 (PubMed:12746542).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB086415 mRNA. Translation: BAC43761.1.
    AB084125 mRNA. Translation: BAC75663.1.

    Genome annotation databases

    KEGGiag:BAC43761.
    ag:BAC75663.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB086415 mRNA. Translation: BAC43761.1.
    AB084125 mRNA. Translation: BAC75663.1.

    3D structure databases

    ProteinModelPortaliQ8H0D2.
    SMRiQ8H0D2. Positions 3-379.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:BAC43761.
    ag:BAC75663.

    Phylogenomic databases

    KOiK12731.

    Enzyme and pathway databases

    BRENDAi2.1.1.160. 1559.

    Family and domain databases

    Gene3Di3.40.50.150. 3 hits.
    InterProiIPR005299. MeTrfase_7.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PfamiPF03492. Methyltransf_7. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDXMT1_COFAR
    AccessioniPrimary (citable) accession number: Q8H0D2
    Secondary accession number(s): Q84PP8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: March 1, 2003
    Last modified: May 11, 2016
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.