ID   Q8H0C9_VIGUN            Unreviewed;       269 AA.
AC   Q8H0C9;
DT   01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2003, sequence version 1.
DT   27-MAR-2024, entry version 86.
DE   SubName: Full=Chitinase {ECO:0000313|EMBL:QCD91182.1};
DE   SubName: Full=Cotyledoneous yieldin-like protein {ECO:0000313|EMBL:BAC53632.1};
GN   Name=VuCYLP {ECO:0000313|EMBL:BAC53632.1};
GN   ORFNames=DEO72_LG4g2146 {ECO:0000313|EMBL:QCD91182.1};
OS   Vigna unguiculata (Cowpea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX   NCBI_TaxID=3917 {ECO:0000313|EMBL:BAC53632.1};
RN   [1] {ECO:0000313|EMBL:BAC53632.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Takahashi K., Okamoto-Nakazato A., Kido N., Katou K.;
RT   "Cotyledonous yieldin-like protein.";
RL   Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007829|PDB:3W3E}
RP   X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 28-269, AND DISULFIDE BONDS.
RA   Morohashi M., Sasaki K., Sakabe N., Sakabe K.;
RT   "Three-dimensional structure analysis of Vigna unguiculata chitinase with
RT   regulation activity of the yield threshold of cell wall.";
RL   Submitted (DEC-2012) to the PDB data bank.
RN   [3] {ECO:0000313|EMBL:QCD91182.1, ECO:0000313|Proteomes:UP000501690}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   TISSUE=Leaf {ECO:0000313|EMBL:QCD91182.1};
RA   Xia Q., Zhang R., Dong Y.;
RT   "An improved genome assembly and genetic linkage map for asparagus bean,
RT   Vigna unguiculata ssp. sesquipedialis.";
RL   Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; AB086859; BAC53632.1; -; mRNA.
DR   EMBL; CP039348; QCD91182.1; -; Genomic_DNA.
DR   PDB; 3W3E; X-ray; 1.50 A; A/B=28-269.
DR   PDBsum; 3W3E; -.
DR   AlphaFoldDB; Q8H0C9; -.
DR   SMR; Q8H0C9; -.
DR   CAZy; GH19; Glycoside Hydrolase Family 19.
DR   Proteomes; UP000501690; Chromosome vu02.
DR   GO; GO:0008061; F:chitin binding; IEA:UniProtKB-KW.
DR   GO; GO:0004568; F:chitinase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0016998; P:cell wall macromolecule catabolic process; IEA:InterPro.
DR   GO; GO:0006032; P:chitin catabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   CDD; cd00325; chitinase_GH19; 1.
DR   Gene3D; 1.10.530.10; -; 1.
DR   Gene3D; 3.30.20.10; Endochitinase, domain 2; 1.
DR   InterPro; IPR016283; Glyco_hydro_19.
DR   InterPro; IPR000726; Glyco_hydro_19_cat.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   PANTHER; PTHR22595:SF171; CHITIN-BINDING TYPE-1 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR22595; CHITINASE-RELATED; 1.
DR   Pfam; PF00182; Glyco_hydro_19; 1.
DR   PIRSF; PIRSF001060; Endochitinase; 1.
DR   SUPFAM; SSF53955; Lysozyme-like; 1.
DR   PROSITE; PS00773; CHITINASE_19_1; 1.
DR   PROSITE; PS00774; CHITINASE_19_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:3W3E};
KW   Chitin-binding {ECO:0000256|ARBA:ARBA00022669};
KW   Disulfide bond {ECO:0000256|PIRSR:PIRSR001060-2};
KW   Plant defense {ECO:0000256|ARBA:ARBA00022821};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           28..269
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5036286575"
FT   DOMAIN          51..73
FT                   /note="Glycoside hydrolase family 19 catalytic"
FT                   /evidence="ECO:0000259|PROSITE:PS00773"
FT   DOMAIN          175..185
FT                   /note="Glycoside hydrolase family 19 catalytic"
FT                   /evidence="ECO:0000259|PROSITE:PS00774"
FT   ACT_SITE        95
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001060-1"
FT   DISULFID        51..113
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001060-2,
FT                   ECO:0007829|PDB:3W3E"
FT   DISULFID        125..131
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001060-2,
FT                   ECO:0007829|PDB:3W3E"
FT   DISULFID        230..262
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001060-2,
FT                   ECO:0007829|PDB:3W3E"
SQ   SEQUENCE   269 AA;  29075 MW;  66AD42978C694E2C CRC64;
     MKLHTFAFAL LAYILVITSS YFRSAVGAEV GSVIGASLFD QLLKHRNDQA CEGKGFYSYN
     AFITAARSFA AFGTTGDSNT RKREVAAFLA QTSHETTGGA ATSPDGPYAW GYCFVTERDK
     SNRYCDGSGP CSAGKSYYGR GPIQLTHNYN YNAAGRALGV DLINNPDLVA RDAVVSFKTA
     LWFWMTPQGN KPSCHDVITN RWTPSAADKA ANRVPGFGVI TNIINGGLEC GKGPTPASGD
     RIGFYKRYCD VFGVSYGPNL NCRDQRPFG
//