ID   LOX6_ORYSJ              Reviewed;         918 AA.
AC   Q8H016; A0A0P0VTS2; Q10QX5; Q8H7L4;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2005, sequence version 2.
DT   27-MAR-2024, entry version 129.
DE   RecName: Full=Probable lipoxygenase 6;
DE            EC=1.13.11.12;
GN   OrderedLocusNames=Os03g0179900 {ECO:0000312|EMBL:BAF11072.1},
GN   LOC_Os03g08220 {ECO:0000312|EMBL:ABF94297.1};
GN   ORFNames=OSJNBa0050H14.14, OSJNBb0076N15.1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Plant lipoxygenase may be involved in a number of diverse
CC       aspects of plant physiology including growth and development, pest
CC       resistance, and senescence or responses to wounding. Catalyzes the
CC       hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene
CC       structure (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)-
CC         octadecadienoate; Xref=Rhea:RHEA:22780, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30245, ChEBI:CHEBI:57466; EC=1.13.11.12;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z,12Z,15Z)-octadecatrienoate + O2 = (13S)-hydroperoxy-
CC         (9Z,11E,15Z)-octadecatrienoate; Xref=Rhea:RHEA:34495,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:32387, ChEBI:CHEBI:58757;
CC         EC=1.13.11.12;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU00726};
CC       Note=Binds 1 Fe cation per subunit. Iron is tightly bound.
CC       {ECO:0000255|PROSITE-ProRule:PRU00726};
CC   -!- PATHWAY: Lipid metabolism; oxylipin biosynthesis. {ECO:0000255|PROSITE-
CC       ProRule:PRU00726}.
CC   -!- SIMILARITY: Belongs to the lipoxygenase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN65431.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAO13474.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC125472; AAO13474.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AC126223; AAN65431.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DP000009; ABF94297.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF11072.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS82610.1; -; Genomic_DNA.
DR   EMBL; AK121637; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015630875.1; XM_015775389.1.
DR   AlphaFoldDB; Q8H016; -.
DR   SMR; Q8H016; -.
DR   STRING; 39947.Q8H016; -.
DR   PaxDb; 39947-Q8H016; -.
DR   EnsemblPlants; Os03t0179900-01; Os03t0179900-01; Os03g0179900.
DR   GeneID; 4331824; -.
DR   Gramene; Os03t0179900-01; Os03t0179900-01; Os03g0179900.
DR   KEGG; osa:4331824; -.
DR   eggNOG; ENOG502QQSP; Eukaryota.
DR   HOGENOM; CLU_004282_0_0_1; -.
DR   InParanoid; Q8H016; -.
DR   OMA; ILIWNAS; -.
DR   OrthoDB; 462210at2759; -.
DR   PlantReactome; R-OSA-1119332; Jasmonic acid biosynthesis.
DR   PlantReactome; R-OSA-1119566; Divinyl ether biosynthesis II (13-LOX).
DR   PlantReactome; R-OSA-1119618; 13-LOX and 13-HPL pathway.
DR   UniPathway; UPA00382; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   ExpressionAtlas; Q8H016; baseline and differential.
DR   GO; GO:0016165; F:linoleate 13S-lipoxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IBA:GO_Central.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0034440; P:lipid oxidation; IBA:GO_Central.
DR   GO; GO:0031408; P:oxylipin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd01751; PLAT_LH2; 1.
DR   Gene3D; 3.10.450.60; -; 1.
DR   Gene3D; 2.60.60.20; PLAT/LH2 domain; 1.
DR   InterPro; IPR000907; LipOase.
DR   InterPro; IPR013819; LipOase_C.
DR   InterPro; IPR036226; LipOase_C_sf.
DR   InterPro; IPR020834; LipOase_CS.
DR   InterPro; IPR020833; LipOase_Fe_BS.
DR   InterPro; IPR001246; LipOase_plant.
DR   InterPro; IPR042057; Lipoxy_PLAT/LH2.
DR   InterPro; IPR027433; Lipoxygenase_dom_3.
DR   InterPro; IPR001024; PLAT/LH2_dom.
DR   InterPro; IPR036392; PLAT/LH2_dom_sf.
DR   PANTHER; PTHR11771; LIPOXYGENASE; 1.
DR   PANTHER; PTHR11771:SF113; LIPOXYGENASE 6-RELATED; 1.
DR   Pfam; PF00305; Lipoxygenase; 1.
DR   Pfam; PF01477; PLAT; 1.
DR   PRINTS; PR00087; LIPOXYGENASE.
DR   PRINTS; PR00468; PLTLPOXGNASE.
DR   SMART; SM00308; LH2; 1.
DR   SUPFAM; SSF49723; Lipase/lipooxygenase domain (PLAT/LH2 domain); 1.
DR   SUPFAM; SSF48484; Lipoxigenase; 1.
DR   PROSITE; PS00711; LIPOXYGENASE_1; 1.
DR   PROSITE; PS00081; LIPOXYGENASE_2; 1.
DR   PROSITE; PS51393; LIPOXYGENASE_3; 1.
DR   PROSITE; PS50095; PLAT; 1.
DR   Genevisible; Q8H016; OS.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Fatty acid biosynthesis; Fatty acid metabolism; Iron;
KW   Lipid biosynthesis; Lipid metabolism; Metal-binding; Oxidoreductase;
KW   Oxylipin biosynthesis; Reference proteome.
FT   CHAIN           1..918
FT                   /note="Probable lipoxygenase 6"
FT                   /id="PRO_0000220712"
FT   DOMAIN          90..218
FT                   /note="PLAT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00152"
FT   DOMAIN          221..918
FT                   /note="Lipoxygenase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00726"
FT   REGION          56..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         573
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00726"
FT   BINDING         578
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00726"
FT   BINDING         765
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00726"
FT   BINDING         769
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00726"
FT   BINDING         918
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00726"
SQ   SEQUENCE   918 AA;  101959 MW;  25BB8DD1E7906CF8 CRC64;
     MELTGLTRAA AAATVTPPAP RRGWGELRFA PLLPGERHGR RKVVVAAISE EVPRLAASPS
     SGIKGGGAGE RRPAPEKVAL RAALTVRRKQ KEDIKEAVAG HLDALWDMVG RNVVLELIST
     KIHPRTKKPM QSGRVSIKDW CQKRGAKGDH VVYTAEFTVD ADFGEPGAIA VANRHNREFF
     LESIVVEGGG LPCGPVHFAC NSWVQSTREL PTKRVFFSNK PYLPSETPPG LRELREKELK
     DLRGDGTGVR KLSDRIYDYA TYNDLGNPDK GKEFIRPILG GEKIPYPRRC RTGRPPTDTN
     MLAESRVEKP HPIYVPRDEA FEELKQGAFS SGRLRAVLHT LIPSLIASIS AETHNFQGFH
     HIDNLYKEGL RLKLGLQEHL FQKIPLVQKI QESSEGMLRY DTPSILSKDK FAWLRDDEFA
     RQAVAGINPV NIERLQVFPP VSKLDPAIYG PPESSITETH IAGHLNGLTV QQAMDEAKLF
     IVDYHDAYLP FLDRINAIDG RKAYATRTIF FLTEAGTLKP IAIELSLPPA KPGEPRPSKV
     LTPPYDATSN WLWMLAKAHV SSNDAGVHQL VNHWLRTHAT MEPFILAAHR HMSAMHPIFK
     LLHPHMRYTL EINALARQSL INADGVIESC FTPGPVSGEI SAAYYRNHWR FDLEGLPSDL
     IRRGVAVEDA TQPHGVRLLI EDYPYANDGL LLWSAIRSWV ESYVQLYYPD AGTVQCDLEL
     QGWYHESIHV GHGDLRHAPW WPPLSTPVDL ASILTTLVWL ASAQHAALNF GQYPLGGYVP
     NRPPLIRRLL PDLERDAAEY AAFLADPHRF FLNAMPGVLE ATKFMAVVDT LSTHSPDEEY
     LGEGRDEGGV PWTADEAAVA AHGMFAADVR RAEETIERRN ADHGRKNRCG AGVLPYELLA
     PSSPPGVTCR GVPNSISI
//