Reviewed, UniProtKB/Swiss-Prot Q8H016 (LOX6_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable lipoxygenase 6
    EC=1.13.11.12

Gene names

Ordered Locus Names:	Os03g0179900, LOC_Os03g08220
ORF Names:	OSJNBa0050H14.14, OSJNBb0076N15.1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	918 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure By similarity.
Catalytic activity	Linoleate + O(2) = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.
Sequence caution	The sequence AAN65431.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAO13474.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 918	918	Probable lipoxygenase 6		PRO_0000220712
Regions
	Domain	90 – 218	129	PLAT
	Domain	221 – 918	698	Lipoxygenase
Sites
	Metal binding	573	1	Iron; catalytic By similarity
	Metal binding	578	1	Iron; catalytic By similarity
	Metal binding	765	1	Iron; catalytic By similarity
	Metal binding	769	1	Iron; catalytic By similarity
	Metal binding	918	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8H016-1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 25BB8DD1E7906CF8	FASTA	918	101,959
	10 20 30 40 50 60 MELTGLTRAA AAATVTPPAP RRGWGELRFA PLLPGERHGR RKVVVAAISE EVPRLAASPS 70 80 90 100 110 120 SGIKGGGAGE RRPAPEKVAL RAALTVRRKQ KEDIKEAVAG HLDALWDMVG RNVVLELIST 130 140 150 160 170 180 KIHPRTKKPM QSGRVSIKDW CQKRGAKGDH VVYTAEFTVD ADFGEPGAIA VANRHNREFF 190 200 210 220 230 240 LESIVVEGGG LPCGPVHFAC NSWVQSTREL PTKRVFFSNK PYLPSETPPG LRELREKELK 250 260 270 280 290 300 DLRGDGTGVR KLSDRIYDYA TYNDLGNPDK GKEFIRPILG GEKIPYPRRC RTGRPPTDTN 310 320 330 340 350 360 MLAESRVEKP HPIYVPRDEA FEELKQGAFS SGRLRAVLHT LIPSLIASIS AETHNFQGFH 370 380 390 400 410 420 HIDNLYKEGL RLKLGLQEHL FQKIPLVQKI QESSEGMLRY DTPSILSKDK FAWLRDDEFA 430 440 450 460 470 480 RQAVAGINPV NIERLQVFPP VSKLDPAIYG PPESSITETH IAGHLNGLTV QQAMDEAKLF 490 500 510 520 530 540 IVDYHDAYLP FLDRINAIDG RKAYATRTIF FLTEAGTLKP IAIELSLPPA KPGEPRPSKV 550 560 570 580 590 600 LTPPYDATSN WLWMLAKAHV SSNDAGVHQL VNHWLRTHAT MEPFILAAHR HMSAMHPIFK 610 620 630 640 650 660 LLHPHMRYTL EINALARQSL INADGVIESC FTPGPVSGEI SAAYYRNHWR FDLEGLPSDL 670 680 690 700 710 720 IRRGVAVEDA TQPHGVRLLI EDYPYANDGL LLWSAIRSWV ESYVQLYYPD AGTVQCDLEL 730 740 750 760 770 780 QGWYHESIHV GHGDLRHAPW WPPLSTPVDL ASILTTLVWL ASAQHAALNF GQYPLGGYVP 790 800 810 820 830 840 NRPPLIRRLL PDLERDAAEY AAFLADPHRF FLNAMPGVLE ATKFMAVVDT LSTHSPDEEY 850 860 870 880 890 900 LGEGRDEGGV PWTADEAAVA AHGMFAADVR RAEETIERRN ADHGRKNRCG AGVLPYELLA 910 PSSPPGVTCR GVPNSISI

References

[1]

"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.-I., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC125472 Genomic DNA. Translation: AAO13474.1. Sequence problems. AC126223 Genomic DNA. Translation: AAN65431.1. Sequence problems. AK121637 mRNA. No translation available.
RefSeq	NP_001049158.1.
UniGene	Os.9616
3D structure databases
HSSP	HSSP built from PDB template 2SBL based on UniProtKB P08170.
ModBase	Search...
Genome annotation databases
GeneID	4331824.
KEGG	osa:4331824.
Organism-specific databases
Gramene	Q8H016.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX6_ORYSJ

Accession

Primary (citable) accession number: Q8H016
Secondary accession number(s): Q8H7L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	November 25, 2008
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)