Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8GZD5 (Q8GZD5_9ROSI) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Xyloglucan endotransglucosylase/hydrolase RuleBase RU361120

EC=2.4.1.207 RuleBase RU361120
Gene names
Name:XET16A EMBL AAN87142.1
OrganismPopulus tremula x Populus tremuloides EMBL AAN87142.1
Taxonomic identifier47664 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity. RuleBase RU361120

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan. RuleBase RU361120

Subcellular location

Secretedcell wall. Secretedextracellular spaceapoplast By similarity RuleBase RU361120.

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity. RuleBase RU361120

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. RuleBase RU361120

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential EMBL AAN87142.1
Chain23 – 294272xyloglucan endotransglycosylase EMBL AAN87142.1
PRO_5000063345

Regions

Region134 – 1363Galactose binding PDB 1UMZ

Sites

Active site1071Nucleophile PDB 1UN1
Active site1111Proton donor/acceptor PDB 1UN1
Site1091Important for catalytic activity PDB 1UN1

Amino acid modifications

Glycosylation1151N-linked (GlcNAc...) PDB 1UN1 PDB 1UMZ

Sequences

Sequence LengthMass (Da)Tools
Q8GZD5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 168017205AF55A4D

FASTA29434,218
        10         20         30         40         50         60 
MAAAYPWTLF LGMLVMVSGT MGAALRKPVD VAFGRNYVPT WAFDHIKYFN GGNEIQLHLD 

        70         80         90        100        110        120 
KYTGTGFQSK GSYLFGHFSM QMKLVPGDSA GTVTAFYLSS QNSEHDEIDF EFLGNRTGQP 

       130        140        150        160        170        180 
YILQTNVFTG GKGDREQRIY LWFDPTKEFH YYSVLWNMYM IVFLVDDVPI RVFKNCKDLG 

       190        200        210        220        230        240 
VKFPFNQPMK IYSSLWNADD WATRGGLEKT DWSKAPFIAS YRSFHIDGCE ASVEAKFCAT 

       250        260        270        280        290 
QGARWWDQKE FQDLDAFQYR RLSWVRQKYT IYNYCTDRSR YPSMPPECKR DRDI 

« Hide

References

[1]"Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues."
Bourquin V., Nishikubo N., Abe H., Brumer H., Denman S., Eklund M., Christiernin M., Teeri T.T., Sundberg B., Mellerowicz E.J.
Plant Cell 14:3073-3088(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]Denman S.E., Eklund M., Teeri T.T.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[3]"Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding."
Johansson P., Brumer H., Baumann M.J., Kallas A.M., Henriksson H., Denman S.E., Teeri T.T., Jones T.A.
Plant Cell 16:874-886(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 23-294 IN COMPLEX WITH GALACTOSE, ACTIVE SITE, GLYCOSYLATION AT ASN-115.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF515607 mRNA. Translation: AAN87142.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UMZX-ray1.80A/B23-294[»]
1UN1X-ray2.10A/B23-294[»]
ProteinModelPortalQ8GZD5.
SMRQ8GZD5. Positions 28-294.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.4.1.207. 275963.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8GZD5.

Entry information

Entry nameQ8GZD5_9ROSI
AccessionPrimary (citable) accession number: Q8GZD5
Entry history
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)