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Protein

Xyloglucan endotransglucosylase/hydrolase

Gene

XET16A

Organism
Populus tremula x Populus tremuloides
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.UniRule annotation

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei107 – 1071NucleophileCombined sources
Sitei109 – 1091Important for catalytic activityCombined sources
Active sitei111 – 1111Proton donor/acceptorCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, GlycosyltransferaseImported, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradationUniRule annotation

Enzyme and pathway databases

BRENDAi2.4.1.207. 4980.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolaseUniRule annotation (EC:2.4.1.207UniRule annotation)
Gene namesi
Name:XET16AImported
OrganismiPopulus tremula x Populus tremuloidesImported
Taxonomic identifieri47664 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

Subcellular locationi

  • Secretedcell wall UniRule annotation
  • Secretedextracellular spaceapoplast UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

ApoplastUniRule annotation, Cell wallUniRule annotation, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222UniRule annotationAdd
BLAST
Signal peptidei1 – 2222 PotentialImportedAdd
BLAST
Chaini23 – 294272Xyloglucan endotransglucosylase/hydrolaseUniRule annotationPRO_5001353595Add
BLAST
Chaini23 – 294272xyloglucan endotransglycosylaseImportedPRO_5000063345Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi115 – 1151N-linked (GlcNAc...)
Disulfide bondi229 ↔ 238
Disulfide bondi275 ↔ 288

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UMZX-ray1.80A/B23-294[»]
1UN1X-ray2.10A/B23-294[»]
ProteinModelPortaliQ8GZD5.
SMRiQ8GZD5. Positions 28-294.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GZD5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1363Galactose binding

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotationImported

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GZD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAYPWTLF LGMLVMVSGT MGAALRKPVD VAFGRNYVPT WAFDHIKYFN
60 70 80 90 100
GGNEIQLHLD KYTGTGFQSK GSYLFGHFSM QMKLVPGDSA GTVTAFYLSS
110 120 130 140 150
QNSEHDEIDF EFLGNRTGQP YILQTNVFTG GKGDREQRIY LWFDPTKEFH
160 170 180 190 200
YYSVLWNMYM IVFLVDDVPI RVFKNCKDLG VKFPFNQPMK IYSSLWNADD
210 220 230 240 250
WATRGGLEKT DWSKAPFIAS YRSFHIDGCE ASVEAKFCAT QGARWWDQKE
260 270 280 290
FQDLDAFQYR RLSWVRQKYT IYNYCTDRSR YPSMPPECKR DRDI
Length:294
Mass (Da):34,218
Last modified:March 1, 2003 - v1
Checksum:i168017205AF55A4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515607 mRNA. Translation: AAN87142.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515607 mRNA. Translation: AAN87142.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UMZX-ray1.80A/B23-294[»]
1UN1X-ray2.10A/B23-294[»]
ProteinModelPortaliQ8GZD5.
SMRiQ8GZD5. Positions 28-294.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.4.1.207. 4980.

Miscellaneous databases

EvolutionaryTraceiQ8GZD5.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues."
    Bourquin V., Nishikubo N., Abe H., Brumer H., Denman S., Eklund M., Christiernin M., Teeri T.T., Sundberg B., Mellerowicz E.J.
    Plant Cell 14:3073-3088(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. Denman S.E., Eklund M., Teeri T.T.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding."
    Johansson P., Brumer H., Baumann M.J., Kallas A.M., Henriksson H., Denman S.E., Teeri T.T., Jones T.A.
    Plant Cell 16:874-886(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 23-294 IN COMPLEX WITH GALACTOSE, ACTIVE SITE, DISULFIDE BONDS, GLYCOSYLATION AT ASN-115.

Entry informationi

Entry nameiQ8GZD5_9ROSI
AccessioniPrimary (citable) accession number: Q8GZD5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: May 27, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structure

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.