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Protein

Xyloglucan endotransglucosylase/hydrolase

Gene

XET16A

Organism
Populus tremula x Populus tremuloides (Hybrid aspen)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.UniRule annotation

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei107NucleophileCombined sources1
Sitei109Important for catalytic activityCombined sources1
Active sitei111Proton donor/acceptorCombined sources1
Binding sitei280GalactoseCombined sources1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, GlycosyltransferaseImported, Hydrolase, Transferase
Biological processCell wall biogenesis/degradationUniRule annotation

Enzyme and pathway databases

BRENDAi2.4.1.207 4980

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolaseUniRule annotation (EC:2.4.1.207UniRule annotation)
Gene namesi
Name:XET16AImported
OrganismiPopulus tremula x Populus tremuloides (Hybrid aspen)Imported
Taxonomic identifieri47664 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

ApoplastUniRule annotation, Cell wallUniRule annotation, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22UniRule annotationAdd BLAST22
ChainiPRO_500514446023 – 294Xyloglucan endotransglucosylase/hydrolaseUniRule annotationAdd BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi115N-linked (GlcNAc...)Combined sources1
Disulfide bondi229 ↔ 238Combined sources
Disulfide bondi275 ↔ 288Combined sources

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UMZX-ray1.80A/B23-294[»]
1UN1X-ray2.10A/B23-294[»]
ProteinModelPortaliQ8GZD5
SMRiQ8GZD5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GZD5

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 221GH16InterPro annotationAdd BLAST202

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 136Galactose bindingCombined sources3

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR008263 GH16_AS
IPR010713 XET_C
IPR016455 XTH
PfamiView protein in Pfam
PF00722 Glyco_hydro_16, 1 hit
PF06955 XET_C, 1 hit
PIRSFiPIRSF005604 XET, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS01034 GH16_1, 1 hit
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GZD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAYPWTLF LGMLVMVSGT MGAALRKPVD VAFGRNYVPT WAFDHIKYFN
60 70 80 90 100
GGNEIQLHLD KYTGTGFQSK GSYLFGHFSM QMKLVPGDSA GTVTAFYLSS
110 120 130 140 150
QNSEHDEIDF EFLGNRTGQP YILQTNVFTG GKGDREQRIY LWFDPTKEFH
160 170 180 190 200
YYSVLWNMYM IVFLVDDVPI RVFKNCKDLG VKFPFNQPMK IYSSLWNADD
210 220 230 240 250
WATRGGLEKT DWSKAPFIAS YRSFHIDGCE ASVEAKFCAT QGARWWDQKE
260 270 280 290
FQDLDAFQYR RLSWVRQKYT IYNYCTDRSR YPSMPPECKR DRDI
Length:294
Mass (Da):34,218
Last modified:March 1, 2003 - v1
Checksum:i168017205AF55A4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515607 mRNA Translation: AAN87142.1

Similar proteinsi

Entry informationi

Entry nameiQ8GZD5_POPPZ
AccessioniPrimary (citable) accession number: Q8GZD5
Entry historyiIntegrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health