Q8GZD5 (Q8GZD5_9ROSI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Xyloglucan endotransglycosylase EMBL AAN87142.1
EC=2.4.1.207 EMBL AAN87142.1

Gene names

Name:

XET16A EMBL AAN87142.1

Organism

Populus tremula x Populus tremuloides EMBL AAN87142.1

Taxonomic identifier

47664 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL AAN87142.1
Molecular function	Glycosyltransferase EMBL AAN87142.1 Transferase
Technical term	3D-structure PDB 1UN1 PDB 1UMZ
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: InterPro cell wall Inferred from electronic annotation. Source: InterPro
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential EMBL AAN87142.1
	Chain	23 – 294	272	xyloglucan endotransglycosylase EMBL AAN87142.1		PRO_5000063345
Regions
	Region	134 – 136	3	Galactose binding PDB 1UMZ
Sites
	Active site	107	1	Nucleophile PDB 1UN1
	Active site	111	1	Proton donor/acceptor PDB 1UN1
	Site	109	1	Important for catalytic activity PDB 1UN1
Amino acid modifications
	Glycosylation	115	1	N-linked (GlcNAc...) PDB 1UN1 PDB 1UMZ

Sequences

	Sequence		Length	Mass (Da)
	Q8GZD5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 168017205AF55A4D	FASTA	294	34,218
	10 20 30 40 50 60 MAAAYPWTLF LGMLVMVSGT MGAALRKPVD VAFGRNYVPT WAFDHIKYFN GGNEIQLHLD 70 80 90 100 110 120 KYTGTGFQSK GSYLFGHFSM QMKLVPGDSA GTVTAFYLSS QNSEHDEIDF EFLGNRTGQP 130 140 150 160 170 180 YILQTNVFTG GKGDREQRIY LWFDPTKEFH YYSVLWNMYM IVFLVDDVPI RVFKNCKDLG 190 200 210 220 230 240 VKFPFNQPMK IYSSLWNADD WATRGGLEKT DWSKAPFIAS YRSFHIDGCE ASVEAKFCAT 250 260 270 280 290 QGARWWDQKE FQDLDAFQYR RLSWVRQKYT IYNYCTDRSR YPSMPPECKR DRDI

References

[1]	"Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues." Bourquin V., Nishikubo N., Abe H., Brumer H., Denman S., Eklund M., Christiernin M., Teeri T.T., Sundberg B., Mellerowicz E.J. Plant Cell 14:3073-3088(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Denman S.E., Eklund M., Teeri T.T. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding." Johansson P., Brumer H., Baumann M.J., Kallas A.M., Henriksson H., Denman S.E., Teeri T.T., Jones T.A. Plant Cell 16:874-886(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 23-294 IN COMPLEX WITH GALACTOSE, ACTIVE SITE, GLYCOSYLATION AT ASN-115.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF515607 mRNA. Translation: AAN87142.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UMZ	X-ray	1.80	A/B	23-294	[»]
1UN1	X-ray	2.10	A/B	23-294	[»]

ProteinModelPortal

Q8GZD5.

SMR

Q8GZD5. Positions 28-294.

ModBase

Search...

Protein family/group databases

CAZy

GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

2.4.1.207. 275963.

Family and domain databases

Gene3D

2.60.120.200. 1 hit.

InterPro

IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]

Pfam

PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]

PIRSF

PIRSF005604. XET. 1 hit.

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

PROSITE

PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8GZD5.

Entry information

Entry name

Q8GZD5_9ROSI

Accession

Primary (citable) accession number: Q8GZD5

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2003
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)