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Protein

Xyloglucan endotransglucosylase/hydrolase

Gene

XET16A

Organism
Populus tremula x Populus tremuloides
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.UniRule annotation

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei107NucleophileCombined sources1
Sitei109Important for catalytic activityCombined sources1
Active sitei111Proton donor/acceptorCombined sources1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, GlycosyltransferaseImported, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradationUniRule annotation

Enzyme and pathway databases

BRENDAi2.4.1.207. 4980.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolaseUniRule annotation (EC:2.4.1.207UniRule annotation)
Gene namesi
Name:XET16AImported
OrganismiPopulus tremula x Populus tremuloidesImported
Taxonomic identifieri47664 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

Subcellular locationi

  • Secretedcell wall UniRule annotation
  • Secretedextracellular spaceapoplast UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

ApoplastUniRule annotation, Cell wallUniRule annotation, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22UniRule annotationAdd BLAST22
ChainiPRO_500514446023 – 294Xyloglucan endotransglucosylase/hydrolaseUniRule annotationAdd BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
GlycosylationiCAR_5005390029115N-linked (GlcNAc...)Combined sources1
Disulfide bondi229 ↔ 238Combined sources
Disulfide bondi275 ↔ 288Combined sources

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.UniRule annotation

Proteomic databases

PRIDEiQ8GZD5.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UMZX-ray1.80A/B23-294[»]
1UN1X-ray2.10A/B23-294[»]
ProteinModelPortaliQ8GZD5.
SMRiQ8GZD5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GZD5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 221GH16 (glycosyl hydrolase family 16)InterPro annotationAdd BLAST202

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 136Galactose bindingCombined sources3

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GZD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAYPWTLF LGMLVMVSGT MGAALRKPVD VAFGRNYVPT WAFDHIKYFN
60 70 80 90 100
GGNEIQLHLD KYTGTGFQSK GSYLFGHFSM QMKLVPGDSA GTVTAFYLSS
110 120 130 140 150
QNSEHDEIDF EFLGNRTGQP YILQTNVFTG GKGDREQRIY LWFDPTKEFH
160 170 180 190 200
YYSVLWNMYM IVFLVDDVPI RVFKNCKDLG VKFPFNQPMK IYSSLWNADD
210 220 230 240 250
WATRGGLEKT DWSKAPFIAS YRSFHIDGCE ASVEAKFCAT QGARWWDQKE
260 270 280 290
FQDLDAFQYR RLSWVRQKYT IYNYCTDRSR YPSMPPECKR DRDI
Length:294
Mass (Da):34,218
Last modified:March 1, 2003 - v1
Checksum:i168017205AF55A4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515607 mRNA. Translation: AAN87142.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515607 mRNA. Translation: AAN87142.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UMZX-ray1.80A/B23-294[»]
1UN1X-ray2.10A/B23-294[»]
ProteinModelPortaliQ8GZD5.
SMRiQ8GZD5.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEiQ8GZD5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.4.1.207. 4980.

Miscellaneous databases

EvolutionaryTraceiQ8GZD5.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ8GZD5_9ROSI
AccessioniPrimary (citable) accession number: Q8GZD5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.