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Q8GZD5

- Q8GZD5_9ROSI

UniProt

Q8GZD5 - Q8GZD5_9ROSI

Protein

Xyloglucan endotransglucosylase/hydrolase

Gene

XET16A

Organism
Populus tremula x Populus tremuloides
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.UniRule annotation

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei107 – 1071NucleophileImported
    Sitei109 – 1091Important for catalytic activityImported
    Active sitei111 – 1111Proton donor/acceptorImported

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseUniRule annotation, GlycosyltransferaseImported, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradationUniRule annotation

    Enzyme and pathway databases

    BRENDAi2.4.1.207. 275963.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolaseUniRule annotation (EC:2.4.1.207UniRule annotation)
    Gene namesi
    Name:XET16AImported
    OrganismiPopulus tremula x Populus tremuloidesImported
    Taxonomic identifieri47664 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

    Subcellular locationi

    Secretedcell wall UniRule annotation. Secretedextracellular spaceapoplast UniRule annotation

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    ApoplastUniRule annotation, Cell wallUniRule annotation, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222 PotentialImportedAdd
    BLAST
    Chaini23 – 294272xyloglucan endotransglycosylaseImportedPRO_5000063345Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi115 – 1151N-linked (GlcNAc...)Imported

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.UniRule annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UMZX-ray1.80A/B23-294[»]
    1UN1X-ray2.10A/B23-294[»]
    ProteinModelPortaliQ8GZD5.
    SMRiQ8GZD5. Positions 28-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8GZD5.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni134 – 1363Galactose bindingImported

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.UniRule annotation

    Keywords - Domaini

    SignalUniRule annotationImported

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GZD5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAYPWTLF LGMLVMVSGT MGAALRKPVD VAFGRNYVPT WAFDHIKYFN    50
    GGNEIQLHLD KYTGTGFQSK GSYLFGHFSM QMKLVPGDSA GTVTAFYLSS 100
    QNSEHDEIDF EFLGNRTGQP YILQTNVFTG GKGDREQRIY LWFDPTKEFH 150
    YYSVLWNMYM IVFLVDDVPI RVFKNCKDLG VKFPFNQPMK IYSSLWNADD 200
    WATRGGLEKT DWSKAPFIAS YRSFHIDGCE ASVEAKFCAT QGARWWDQKE 250
    FQDLDAFQYR RLSWVRQKYT IYNYCTDRSR YPSMPPECKR DRDI 294
    Length:294
    Mass (Da):34,218
    Last modified:March 1, 2003 - v1
    Checksum:i168017205AF55A4D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF515607 mRNA. Translation: AAN87142.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF515607 mRNA. Translation: AAN87142.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UMZ X-ray 1.80 A/B 23-294 [» ]
    1UN1 X-ray 2.10 A/B 23-294 [» ]
    ProteinModelPortali Q8GZD5.
    SMRi Q8GZD5. Positions 28-294.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.4.1.207. 275963.

    Miscellaneous databases

    EvolutionaryTracei Q8GZD5.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues."
      Bourquin V., Nishikubo N., Abe H., Brumer H., Denman S., Eklund M., Christiernin M., Teeri T.T., Sundberg B., Mellerowicz E.J.
      Plant Cell 14:3073-3088(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. Denman S.E., Eklund M., Teeri T.T.
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding."
      Johansson P., Brumer H., Baumann M.J., Kallas A.M., Henriksson H., Denman S.E., Teeri T.T., Jones T.A.
      Plant Cell 16:874-886(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 23-294 IN COMPLEX WITH GALACTOSE, ACTIVE SITE, GLYCOSYLATION AT ASN-115.

    Entry informationi

    Entry nameiQ8GZD5_9ROSI
    AccessioniPrimary (citable) accession number: Q8GZD5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 1, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3