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Q8GZD5

- Q8GZD5_9ROSI

UniProt

Q8GZD5 - Q8GZD5_9ROSI

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Protein

Xyloglucan endotransglucosylase/hydrolase

Gene

XET16A

Organism
Populus tremula x Populus tremuloides
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.UniRule annotation

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei107 – 1071NucleophileImported
Sitei109 – 1091Important for catalytic activityImported
Active sitei111 – 1111Proton donor/acceptorImported

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, GlycosyltransferaseImported, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradationUniRule annotation

Enzyme and pathway databases

BRENDAi2.4.1.207. 275963.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolaseUniRule annotation (EC:2.4.1.207UniRule annotation)
Gene namesi
Name:XET16AImported
OrganismiPopulus tremula x Populus tremuloidesImported
Taxonomic identifieri47664 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus

Subcellular locationi

Secretedcell wall UniRule annotation. Secretedextracellular spaceapoplast UniRule annotation

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

ApoplastUniRule annotation, Cell wallUniRule annotation, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 PotentialImportedAdd
BLAST
Chaini23 – 294272xyloglucan endotransglycosylaseImportedPRO_5000063345Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi115 – 1151N-linked (GlcNAc...)Imported

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UMZX-ray1.80A/B23-294[»]
1UN1X-ray2.10A/B23-294[»]
ProteinModelPortaliQ8GZD5.
SMRiQ8GZD5. Positions 28-294.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GZD5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1363Galactose bindingImported

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotationImported

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GZD5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAYPWTLF LGMLVMVSGT MGAALRKPVD VAFGRNYVPT WAFDHIKYFN
60 70 80 90 100
GGNEIQLHLD KYTGTGFQSK GSYLFGHFSM QMKLVPGDSA GTVTAFYLSS
110 120 130 140 150
QNSEHDEIDF EFLGNRTGQP YILQTNVFTG GKGDREQRIY LWFDPTKEFH
160 170 180 190 200
YYSVLWNMYM IVFLVDDVPI RVFKNCKDLG VKFPFNQPMK IYSSLWNADD
210 220 230 240 250
WATRGGLEKT DWSKAPFIAS YRSFHIDGCE ASVEAKFCAT QGARWWDQKE
260 270 280 290
FQDLDAFQYR RLSWVRQKYT IYNYCTDRSR YPSMPPECKR DRDI
Length:294
Mass (Da):34,218
Last modified:March 1, 2003 - v1
Checksum:i168017205AF55A4D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF515607 mRNA. Translation: AAN87142.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF515607 mRNA. Translation: AAN87142.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1UMZ X-ray 1.80 A/B 23-294 [» ]
1UN1 X-ray 2.10 A/B 23-294 [» ]
ProteinModelPortali Q8GZD5.
SMRi Q8GZD5. Positions 28-294.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.4.1.207. 275963.

Miscellaneous databases

EvolutionaryTracei Q8GZD5.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues."
    Bourquin V., Nishikubo N., Abe H., Brumer H., Denman S., Eklund M., Christiernin M., Teeri T.T., Sundberg B., Mellerowicz E.J.
    Plant Cell 14:3073-3088(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. Denman S.E., Eklund M., Teeri T.T.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding."
    Johansson P., Brumer H., Baumann M.J., Kallas A.M., Henriksson H., Denman S.E., Teeri T.T., Jones T.A.
    Plant Cell 16:874-886(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 23-294 IN COMPLEX WITH GALACTOSE, ACTIVE SITE, GLYCOSYLATION AT ASN-115.

Entry informationi

Entry nameiQ8GZD5_9ROSI
AccessioniPrimary (citable) accession number: Q8GZD5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3