Q8GZD4 (NAT3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleobase-ascorbate transporter 3
Short name=AtNAT3

Gene names

Name:	NAT3
Ordered Locus Names:	At2g26510
ORF Names:	T9J22.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed in the apical meristem 4 days after imbibition (DAI). Expressed in the major veins of rosette leaves and pedicels. Expressed in the root central cylinder, root meristems, root tips and lateral root primordia. Ref.5
Developmental stage	Expressed in gynoecium development, disappearing after pollination. Ref.5
Sequence similarities	Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 17317660PubMed 22923678. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8GZD4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Nucleobase-ascorbate transporter 3		PRO_0000270160
Regions
	Transmembrane	56 – 76	21	Helical; Potential
	Transmembrane	92 – 111	20	Helical; Potential
	Transmembrane	117 – 136	20	Helical; Potential
	Transmembrane	158 – 178	21	Helical; Potential
	Transmembrane	179 – 199	21	Helical; Potential
	Transmembrane	202 – 222	21	Helical; Potential
	Transmembrane	242 – 262	21	Helical; Potential
	Transmembrane	306 – 326	21	Helical; Potential
	Transmembrane	390 – 410	21	Helical; Potential
	Transmembrane	412 – 432	21	Helical; Potential
	Transmembrane	442 – 462	21	Helical; Potential
	Transmembrane	481 – 501	21	Helical; Potential
Experimental info
	Sequence conflict	166 – 168	3	NII → TSL in AAO13361. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 52A67E990C3AA2CC	FASTA	551	60,170
	10 20 30 40 50 60 MVETGHHHQH PPAPAAAGHP PVPSMAMARN MGTTWPPAEQ LHHLQYCIHS NPSWHETVVL 70 80 90 100 110 120 AFQHYIVMLG TTVLIANTLV SPMGGDPGDK ARVIQTILFM SGINTLLQTL IGTRLPTVMG 130 140 150 160 170 180 VSFAYVLPVL SIIRDYNNGQ FDSEKQRFRH TMRTVQGSLI ISSFVNIIIG YGQAWGNLIR 190 200 210 220 230 240 IFSPIIVVPV VSVVSLGLFL RGFPLLANCV EIGLPMLILL IITQQYLKHA FSRISMILER 250 260 270 280 290 300 YALLVCLAII WAFAAILTVS GAYNNVSTAT KQSCRTDRAF LMSSAPWIRI PYPFQWGTPI 310 320 330 340 350 360 FKASHVFGMF GAAIVASAES TGVFFAASRL AGATAPPAHV VSRSIGLQGI GVLLEGIFGS 370 380 390 400 410 420 ITGNTASVEN VGLLGLTRIG SRRVVQVSTF FMIFFSIFGK FGAFFASIPL PIFAGVYCIL 430 440 450 460 470 480 LGIVVAVGIS FIQFTDTNSM RNMYVIGVSL FLSLSIAQYF LANTSRAGYG PVRTAGGWFN 490 500 510 520 530 540 DILNTIFASA PLVATILATI LDNTLEARHA SDDARGIPWW KPFQHRNGDG RNDEFYSMPL 550 RINELMPTRF L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a putative membrane transporter cDNA of Arabidopsis thaliana." Park S., Thornburg R.W. Hangug Nonghwahag Hoeji 45:153-159(2002) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Identification and expression analysis of twelve members of the nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis thaliana." Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I. Plant Cell Physiol. 47:1381-1393(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF515778 mRNA. Translation: AAO13361.1. AC002505 Genomic DNA. Translation: AAC14499.1. CP002685 Genomic DNA. Translation: AEC07848.1. AF370518 mRNA. Translation: AAK43895.1. BT008902 mRNA. Translation: AAP68341.1.
IPI	IPI00525278.
PIR	T00984.
RefSeq	NP_180219.1. NM_128208.4.
UniGene	At.12385.
3D structure databases
ProteinModelPortal	Q8GZD4.
SMR	Q8GZD4. Positions 48-458.
ModBase	Search...
Proteomic databases
PaxDb	Q8GZD4.
PRIDE	Q8GZD4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G26510.1; AT2G26510.1; AT2G26510.
GeneID	817192.
KEGG	ath:AT2G26510.
Organism-specific databases
TAIR	At2g26510.
Phylogenomic databases
eggNOG	COG2233.
HOGENOM	HOG000038202.
InParanoid	Q8GZD4.
OMA	DAHAIVP.
PhylomeDB	Q8GZD4.
ProtClustDB	CLSN2683307.
Gene expression databases
ArrayExpress	Q8GZD4.
Genevestigator	Q8GZD4.
Family and domain databases
InterPro	IPR006043. Xant/urac/vitC. [Graphical view]
PANTHER	PTHR11119. PTHR11119. 1 hit.
Pfam	PF00860. Xan_ur_permease. 1 hit. [Graphical view]
PROSITE	PS01116. XANTH_URACIL_PERMASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAT3_ARATH

Accession

Primary (citable) accession number: Q8GZD4
Secondary accession number(s): O48718

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program