Reviewed, UniProtKB/Swiss-Prot Q8GZ45 (SYTC_ARATH)

Names and origin

Protein names

Recommended name:
    Probable threonyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Ordered Locus Names:	At1g17960
ORF Names:	F2H15.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8GZ45-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8GZ45-2)

The sequence of this isoform differs from the canonical sequence as follows:
     169-186: KLPIRMAEFGVLHRNEDS → SVAYISFADIFFVLRYGM
     187-458: Missing.

Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Probable threonyl-tRNA synthetase, cytoplasmic		PRO_0000101123
Natural variations
	Alternative sequence	169 – 186	18	KLPIR…RNEDS → SVAYISFADIFFVLRYGM in isoform 2.		VSP_017125
	Alternative sequence	187 – 458	272	Missing in isoform 2.		VSP_017126

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: BBFBC833E5AE0608	FASTA	458	52,436
	10 20 30 40 50 60 MANNHHPKDE AYLSSVIPKR IRLFEEIQAE QLEQLQSRPH DPIKITLLPD GIEKEGRRWE 70 80 90 100 110 120 TSPMDIAVQI SKGLAKSALV SSVNHVLWDM NRPLEGDCSL EIFGFDSDQG RNTFWHSSAH 130 140 150 160 170 180 ILGQALEQEY GCKLCIGPCE PRDEGFYYDS LYYGELGLND NHFPNIEAKL PIRMAEFGVL 190 200 210 220 230 240 HRNEDSGALG GMTRVRRFVQ DDAHIFCRVD QVEEEVKGVL DFIDYVYRIF GFTYELTLST 250 260 270 280 290 300 RPKDHIGDLE TWAKAENDLE KALDDFGKPW VIKEGDGAFY GPKIDITVSD ARNRKFQCAT 310 320 330 340 350 360 IQLDFQLPAC FKLKYLSEKN EMEAPVMIHS AVLGSIERMF AILLEHYKGI WPFWLSPRQA 370 380 390 400 410 420 IVCSLSEDCS SYAKQVQKQI NEVGYYVDID ESDRSLRKKV ADARDAPYNY ILVVGQKEVA 430 440 450 TGQVTVRLRE DPEGRKDLPE MSIESLLDEF KFKTVNFL
	Isoform 2. Checksum: 8CA4DBDD4EF3E120	FASTA	186	21,211
	10 20 30 40 50 60 MANNHHPKDE AYLSSVIPKR IRLFEEIQAE QLEQLQSRPH DPIKITLLPD GIEKEGRRWE 70 80 90 100 110 120 TSPMDIAVQI SKGLAKSALV SSVNHVLWDM NRPLEGDCSL EIFGFDSDQG RNTFWHSSAH 130 140 150 160 170 180 ILGQALEQEY GCKLCIGPCE PRDEGFYYDS LYYGELGLND NHFPNIEASV AYISFADIFF VLRYGM

References

[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC034106 Genomic DNA. Translation: AAF97275.1. AK117218 mRNA. Translation: BAC41894.1.
IPI	IPI00541770. IPI00543069.
PIR	H86314.
RefSeq	NP_173238.1.
UniGene	At.41808
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q8GZ45.
Genome annotation databases
GeneID	838375.
GenomeReviews	Gene locus AT1G17960 in contig CT485782_GR.
KEGG	ath:AT1G17960.
NMPDR	fig|3702.1.peg.2128.
Organism-specific databases
GeneFarm	2473. 210.
TAIR	At1g17960.
Phylogenomic databases
eggNOG	KOG1637.
HOGENOM	HBG352811.
InParanoid	Q8GZ45.
OMA	MEFIREE.
PhylomeDB	Q8GZ45.
Enzyme and pathway databases
BRENDA	6.1.1.3. 302.
Gene expression databases
Genevestigator	Q8GZ45.
GermOnline	AT1G17960. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR012676. TGS-like. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYTC_ARATH

Accession

Primary (citable) accession number: Q8GZ45
Secondary accession number(s): Q9LMT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	February 9, 2010
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)