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Protein

Mitogen-activated protein kinase 12

Gene

MPK12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of the auxin transduction signaling pathway.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70ATPPROSITE-ProRule annotation1
Active sitei167Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 55ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • MAP kinase activity Source: TAIR
  • protein tyrosine kinase activity Source: InterPro

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • intracellular signal transduction Source: TAIR
  • response to auxin Source: TAIR
  • response to indolebutyric acid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Auxin signaling pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 12 (EC:2.7.11.24)
Short name:
AtMPK12
Short name:
MAP kinase 12
Gene namesi
Name:MPK12
Ordered Locus Names:At2g46070
ORF Names:T3F17.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G46070.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458121 – 372Mitogen-activated protein kinase 12Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199PhosphothreonineBy similarity1
Modified residuei201PhosphotyrosineBy similarity1
Modified residuei204PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme (By similarity). Activated by auxin. Dephosphorylated and inactivated by IBR5.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8GYQ5.
PRIDEiQ8GYQ5.

Expressioni

Tissue specificityi

Expressed in seedlings, roots, stems, leaves, flowers and siliques, mostly around stomata.1 Publication

Gene expression databases

ExpressionAtlasiQ8GYQ5. baseline and differential.
GenevisibleiQ8GYQ5. AT.

Interactioni

Subunit structurei

Interacts with IBR5/SKIP33.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
IBR5Q84JU48EBI-2128461,EBI-604555
MKK1Q94A062EBI-2128461,EBI-994464
MKK6Q9FJV02EBI-2128461,EBI-1238868
MKK9Q9FX432EBI-2128461,EBI-2128545

Protein-protein interaction databases

BioGridi4550. 3 interactors.
IntActiQ8GYQ5. 6 interactors.
STRINGi3702.AT2G46070.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GYQ5.
SMRiQ8GYQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 327Protein kinasePROSITE-ProRule annotationAdd BLAST287

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 372IBR5 bindingAdd BLAST163

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi199 – 201TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ8GYQ5.
KOiK04464.
OMAiKQQFAAR.
OrthoDBiEOG09360BXQ.
PhylomeDBiQ8GYQ5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8GYQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGESSSGST EHCIKVVPTH GGRYVQYNVY GQLFEVSRKY VPPIRPIGRG
60 70 80 90 100
ACGIVCAAVN SVTGEKVAIK KIGNAFDNII DAKRTLREIK LLRHMDHENV
110 120 130 140 150
ITIKDIVRPP QRDIFNDVYI VYELMDTDLQ RILRSNQTLT SDQCRFLVYQ
160 170 180 190 200
LLRGLKYVHS ANILHRDLRP SNVLLNSKNE LKIGDFGLAR TTSDTDFMTE
210 220 230 240 250
YVVTRWYRAP ELLLNCSEYT AAIDIWSVGC ILGEIMTGQP LFPGKDYVHQ
260 270 280 290 300
LRLITELVGS PDNSSLGFLR SDNARRYVRQ LPRYPKQQFA ARFPKMPTTA
310 320 330 340 350
IDLLERMLVF DPNRRISVDE ALGHAYLSPH HDVAKEPVCS TPFSFDFEHP
360 370
SCTEEHIKEL IYKESVKFNP DH
Length:372
Mass (Da):42,475
Last modified:March 1, 2003 - v1
Checksum:i1A8FF099D1DC80B9
GO

Sequence cautioni

The sequence AAC62906 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005397 Genomic DNA. Translation: AAC62906.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC10639.1.
AK117449 mRNA. Translation: BAC42114.1.
BT024898 mRNA. Translation: ABD85169.1.
PIRiD84898.
RefSeqiNP_182131.2. NM_130170.4. [Q8GYQ5-1]
UniGeneiAt.36555.

Genome annotation databases

EnsemblPlantsiAT2G46070.1; AT2G46070.1; AT2G46070. [Q8GYQ5-1]
GeneIDi819215.
GrameneiAT2G46070.1; AT2G46070.1; AT2G46070.
KEGGiath:AT2G46070.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005397 Genomic DNA. Translation: AAC62906.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC10639.1.
AK117449 mRNA. Translation: BAC42114.1.
BT024898 mRNA. Translation: ABD85169.1.
PIRiD84898.
RefSeqiNP_182131.2. NM_130170.4. [Q8GYQ5-1]
UniGeneiAt.36555.

3D structure databases

ProteinModelPortaliQ8GYQ5.
SMRiQ8GYQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4550. 3 interactors.
IntActiQ8GYQ5. 6 interactors.
STRINGi3702.AT2G46070.1.

Proteomic databases

PaxDbiQ8GYQ5.
PRIDEiQ8GYQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G46070.1; AT2G46070.1; AT2G46070. [Q8GYQ5-1]
GeneIDi819215.
GrameneiAT2G46070.1; AT2G46070.1; AT2G46070.
KEGGiath:AT2G46070.

Organism-specific databases

TAIRiAT2G46070.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ8GYQ5.
KOiK04464.
OMAiKQQFAAR.
OrthoDBiEOG09360BXQ.
PhylomeDBiQ8GYQ5.

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ8GYQ5.

Gene expression databases

ExpressionAtlasiQ8GYQ5. baseline and differential.
GenevisibleiQ8GYQ5. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK12_ARATH
AccessioniPrimary (citable) accession number: Q8GYQ5
Secondary accession number(s): O82361
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.