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Q8GYQ5

- MPK12_ARATH

UniProt

Q8GYQ5 - MPK12_ARATH

Protein

Mitogen-activated protein kinase 12

Gene

MPK12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Negative regulator of the auxin transduction signaling pathway.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei70 – 701ATPPROSITE-ProRule annotation
    Active sitei167 – 1671Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi47 – 559ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kinase activity Source: TAIR
    3. MAP kinase activity Source: TAIR
    4. protein binding Source: UniProtKB
    5. protein tyrosine kinase activity Source: InterPro

    GO - Biological processi

    1. auxin-activated signaling pathway Source: UniProtKB-KW
    2. MAPK cascade Source: GOC
    3. response to auxin Source: TAIR
    4. response to indolebutyric acid Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Auxin signaling pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G46070-MONOMER.
    ARA:GQT-1132-MONOMER.
    ReactomeiREACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 12 (EC:2.7.11.24)
    Short name:
    AtMPK12
    Short name:
    MAP kinase 12
    Gene namesi
    Name:MPK12
    Ordered Locus Names:At2g46070
    ORF Names:T3F17.28
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G46070.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Mitogen-activated protein kinase 12PRO_0000245812Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei199 – 1991PhosphothreonineBy similarity
    Modified residuei201 – 2011PhosphotyrosineBy similarity
    Modified residuei204 – 2041PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme By similarity. Activated by auxin. Dephosphorylated and inactivated by IBR5.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8GYQ5.
    PRIDEiQ8GYQ5.

    Expressioni

    Tissue specificityi

    Expressed in seedlings, roots, stems, leaves, flowers and siliques, mostly around stomata.1 Publication

    Gene expression databases

    GenevestigatoriQ8GYQ5.

    Interactioni

    Subunit structurei

    Interacts with IBR5/SKIP33.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IBR5Q84JU48EBI-2128461,EBI-604555
    MKK1Q94A062EBI-2128461,EBI-994464
    MKK6Q9FJV02EBI-2128461,EBI-1238868
    MKK9Q9FX432EBI-2128461,EBI-2128545

    Protein-protein interaction databases

    BioGridi4550. 2 interactions.
    IntActiQ8GYQ5. 6 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GYQ5.
    SMRiQ8GYQ5. Positions 6-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 327287Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni210 – 372163IBR5 bindingAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi199 – 2013TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ8GYQ5.
    KOiK04371.
    OMAiHAYLSPH.
    PhylomeDBiQ8GYQ5.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8GYQ5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSGESSSGST EHCIKVVPTH GGRYVQYNVY GQLFEVSRKY VPPIRPIGRG    50
    ACGIVCAAVN SVTGEKVAIK KIGNAFDNII DAKRTLREIK LLRHMDHENV 100
    ITIKDIVRPP QRDIFNDVYI VYELMDTDLQ RILRSNQTLT SDQCRFLVYQ 150
    LLRGLKYVHS ANILHRDLRP SNVLLNSKNE LKIGDFGLAR TTSDTDFMTE 200
    YVVTRWYRAP ELLLNCSEYT AAIDIWSVGC ILGEIMTGQP LFPGKDYVHQ 250
    LRLITELVGS PDNSSLGFLR SDNARRYVRQ LPRYPKQQFA ARFPKMPTTA 300
    IDLLERMLVF DPNRRISVDE ALGHAYLSPH HDVAKEPVCS TPFSFDFEHP 350
    SCTEEHIKEL IYKESVKFNP DH 372
    Length:372
    Mass (Da):42,475
    Last modified:March 1, 2003 - v1
    Checksum:i1A8FF099D1DC80B9
    GO

    Sequence cautioni

    The sequence AAC62906.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005397 Genomic DNA. Translation: AAC62906.1. Different initiation.
    CP002685 Genomic DNA. Translation: AEC10639.1.
    AK117449 mRNA. Translation: BAC42114.1.
    BT024898 mRNA. Translation: ABD85169.1.
    PIRiD84898.
    RefSeqiNP_182131.2. NM_130170.3. [Q8GYQ5-1]
    UniGeneiAt.36555.

    Genome annotation databases

    EnsemblPlantsiAT2G46070.1; AT2G46070.1; AT2G46070. [Q8GYQ5-1]
    GeneIDi819215.
    KEGGiath:AT2G46070.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005397 Genomic DNA. Translation: AAC62906.1 . Different initiation.
    CP002685 Genomic DNA. Translation: AEC10639.1 .
    AK117449 mRNA. Translation: BAC42114.1 .
    BT024898 mRNA. Translation: ABD85169.1 .
    PIRi D84898.
    RefSeqi NP_182131.2. NM_130170.3. [Q8GYQ5-1 ]
    UniGenei At.36555.

    3D structure databases

    ProteinModelPortali Q8GYQ5.
    SMRi Q8GYQ5. Positions 6-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4550. 2 interactions.
    IntActi Q8GYQ5. 6 interactions.

    Proteomic databases

    PaxDbi Q8GYQ5.
    PRIDEi Q8GYQ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G46070.1 ; AT2G46070.1 ; AT2G46070 . [Q8GYQ5-1 ]
    GeneIDi 819215.
    KEGGi ath:AT2G46070.

    Organism-specific databases

    GeneFarmi 856. 89.
    TAIRi AT2G46070.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q8GYQ5.
    KOi K04371.
    OMAi HAYLSPH.
    PhylomeDBi Q8GYQ5.

    Enzyme and pathway databases

    BioCyci ARA:AT2G46070-MONOMER.
    ARA:GQT-1132-MONOMER.
    Reactomei REACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Gene expression databases

    Genevestigatori Q8GYQ5.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Ecker J.R.
      Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. Cited for: GENE FAMILY.
    7. "Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling."
      Lee J.S., Wang S., Sritubtim S., Chen J.-G., Ellis B.E.
      Plant J. 57:975-985(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ACTIVATION, INTERACTION WITH IBR5/SKIP33.

    Entry informationi

    Entry nameiMPK12_ARATH
    AccessioniPrimary (citable) accession number: Q8GYQ5
    Secondary accession number(s): O82361
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3