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Q8GYQ5

- MPK12_ARATH

UniProt

Q8GYQ5 - MPK12_ARATH

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Protein
Mitogen-activated protein kinase 12
Gene
MPK12, At2g46070, T3F17.28
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Negative regulator of the auxin transduction signaling pathway.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701ATP By similarity
Active sitei167 – 1671Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 559ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR
  3. kinase activity Source: TAIR
  4. protein binding Source: UniProtKB
  5. protein tyrosine kinase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. MAPK cascade Source: GOC
  2. auxin-activated signaling pathway Source: UniProtKB-KW
  3. response to auxin Source: TAIR
  4. response to indolebutyric acid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Auxin signaling pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G46070-MONOMER.
ARA:GQT-1132-MONOMER.
ReactomeiREACT_190855. CREB phosphorylation through the activation of Ras.
REACT_190946. KSRP destabilizes mRNA.
REACT_202229. ERK1 activation.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_216613. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 12 (EC:2.7.11.24)
Short name:
AtMPK12
Short name:
MAP kinase 12
Gene namesi
Name:MPK12
Ordered Locus Names:At2g46070
ORF Names:T3F17.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G46070.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Mitogen-activated protein kinase 12
PRO_0000245812Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991Phosphothreonine By similarity
Modified residuei201 – 2011Phosphotyrosine By similarity
Modified residuei204 – 2041Phosphothreonine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme By similarity. Activated by auxin. Dephosphorylated and inactivated by IBR5.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8GYQ5.
PRIDEiQ8GYQ5.

Expressioni

Tissue specificityi

Expressed in seedlings, roots, stems, leaves, flowers and siliques, mostly around stomata.1 Publication

Gene expression databases

GenevestigatoriQ8GYQ5.

Interactioni

Subunit structurei

Interacts with IBR5/SKIP33.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
IBR5Q84JU48EBI-2128461,EBI-604555
MKK1Q94A062EBI-2128461,EBI-994464
MKK6Q9FJV02EBI-2128461,EBI-1238868
MKK9Q9FX432EBI-2128461,EBI-2128545

Protein-protein interaction databases

BioGridi4550. 2 interactions.
IntActiQ8GYQ5. 6 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8GYQ5.
SMRiQ8GYQ5. Positions 6-370.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 327287Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni210 – 372163IBR5 binding
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi199 – 2013TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ8GYQ5.
KOiK04371.
OMAiHAYLSPH.
PhylomeDBiQ8GYQ5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8GYQ5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSGESSSGST EHCIKVVPTH GGRYVQYNVY GQLFEVSRKY VPPIRPIGRG    50
ACGIVCAAVN SVTGEKVAIK KIGNAFDNII DAKRTLREIK LLRHMDHENV 100
ITIKDIVRPP QRDIFNDVYI VYELMDTDLQ RILRSNQTLT SDQCRFLVYQ 150
LLRGLKYVHS ANILHRDLRP SNVLLNSKNE LKIGDFGLAR TTSDTDFMTE 200
YVVTRWYRAP ELLLNCSEYT AAIDIWSVGC ILGEIMTGQP LFPGKDYVHQ 250
LRLITELVGS PDNSSLGFLR SDNARRYVRQ LPRYPKQQFA ARFPKMPTTA 300
IDLLERMLVF DPNRRISVDE ALGHAYLSPH HDVAKEPVCS TPFSFDFEHP 350
SCTEEHIKEL IYKESVKFNP DH 372
Length:372
Mass (Da):42,475
Last modified:March 1, 2003 - v1
Checksum:i1A8FF099D1DC80B9
GO

Sequence cautioni

The sequence AAC62906.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005397 Genomic DNA. Translation: AAC62906.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC10639.1.
AK117449 mRNA. Translation: BAC42114.1.
BT024898 mRNA. Translation: ABD85169.1.
PIRiD84898.
RefSeqiNP_182131.2. NM_130170.3. [Q8GYQ5-1]
UniGeneiAt.36555.

Genome annotation databases

EnsemblPlantsiAT2G46070.1; AT2G46070.1; AT2G46070. [Q8GYQ5-1]
GeneIDi819215.
KEGGiath:AT2G46070.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005397 Genomic DNA. Translation: AAC62906.1 . Different initiation.
CP002685 Genomic DNA. Translation: AEC10639.1 .
AK117449 mRNA. Translation: BAC42114.1 .
BT024898 mRNA. Translation: ABD85169.1 .
PIRi D84898.
RefSeqi NP_182131.2. NM_130170.3. [Q8GYQ5-1 ]
UniGenei At.36555.

3D structure databases

ProteinModelPortali Q8GYQ5.
SMRi Q8GYQ5. Positions 6-370.
ModBasei Search...

Protein-protein interaction databases

BioGridi 4550. 2 interactions.
IntActi Q8GYQ5. 6 interactions.

Proteomic databases

PaxDbi Q8GYQ5.
PRIDEi Q8GYQ5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G46070.1 ; AT2G46070.1 ; AT2G46070 . [Q8GYQ5-1 ]
GeneIDi 819215.
KEGGi ath:AT2G46070.

Organism-specific databases

GeneFarmi 856. 89.
TAIRi AT2G46070.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q8GYQ5.
KOi K04371.
OMAi HAYLSPH.
PhylomeDBi Q8GYQ5.

Enzyme and pathway databases

BioCyci ARA:AT2G46070-MONOMER.
ARA:GQT-1132-MONOMER.
Reactomei REACT_190855. CREB phosphorylation through the activation of Ras.
REACT_190946. KSRP destabilizes mRNA.
REACT_202229. ERK1 activation.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_216613. Signalling to ERK5.

Gene expression databases

Genevestigatori Q8GYQ5.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: GENE FAMILY.
  7. "Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling."
    Lee J.S., Wang S., Sritubtim S., Chen J.-G., Ellis B.E.
    Plant J. 57:975-985(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ACTIVATION, INTERACTION WITH IBR5/SKIP33.

Entry informationi

Entry nameiMPK12_ARATH
AccessioniPrimary (citable) accession number: Q8GYQ5
Secondary accession number(s): O82361
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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