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Protein

Mitogen-activated protein kinase 12

Gene

MPK12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negative regulator of the auxin transduction signaling pathway.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70ATPPROSITE-ProRule annotation1
Active sitei167Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 55ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • MAP kinase activity Source: TAIR
  • protein tyrosine kinase activity Source: InterPro

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • intracellular signal transduction Source: TAIR
  • regulation of gene expression Source: GO_Central
  • response to auxin Source: TAIR
  • response to indolebutyric acid Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processAuxin signaling pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-198753 ERK/MAPK targets
R-ATH-198765 Signalling to ERK5
R-ATH-202670 ERKs are inactivated
R-ATH-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-ATH-881907 Gastrin-CREB signalling pathway via PKC and MAPK

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 12 (EC:2.7.11.24)
Short name:
AtMPK12
Short name:
MAP kinase 12
Gene namesi
Name:MPK12
Ordered Locus Names:At2g46070
ORF Names:T3F17.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G46070
TAIRilocus:2062897 AT2G46070

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458121 – 372Mitogen-activated protein kinase 12Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199PhosphothreonineBy similarity1
Modified residuei201PhosphotyrosineBy similarity1
Modified residuei204PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme (By similarity). Activated by auxin. Dephosphorylated and inactivated by IBR5.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8GYQ5
PRIDEiQ8GYQ5

PTM databases

iPTMnetiQ8GYQ5

Expressioni

Tissue specificityi

Expressed in seedlings, roots, stems, leaves, flowers and siliques, mostly around stomata.1 Publication

Gene expression databases

ExpressionAtlasiQ8GYQ5 baseline and differential
GenevisibleiQ8GYQ5 AT

Interactioni

Subunit structurei

Interacts with IBR5/SKIP33.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4550, 3 interactors
IntActiQ8GYQ5, 6 interactors
STRINGi3702.AT2G46070.1

Structurei

3D structure databases

ProteinModelPortaliQ8GYQ5
SMRiQ8GYQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 327Protein kinasePROSITE-ProRule annotationAdd BLAST287

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 372IBR5 bindingAdd BLAST163

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi199 – 201TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOGENOMiHOG000233024
InParanoidiQ8GYQ5
KOiK04464
OMAiLLSMGEY
OrthoDBiEOG09360BXQ
PhylomeDBiQ8GYQ5

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00219 TyrKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8GYQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGESSSGST EHCIKVVPTH GGRYVQYNVY GQLFEVSRKY VPPIRPIGRG
60 70 80 90 100
ACGIVCAAVN SVTGEKVAIK KIGNAFDNII DAKRTLREIK LLRHMDHENV
110 120 130 140 150
ITIKDIVRPP QRDIFNDVYI VYELMDTDLQ RILRSNQTLT SDQCRFLVYQ
160 170 180 190 200
LLRGLKYVHS ANILHRDLRP SNVLLNSKNE LKIGDFGLAR TTSDTDFMTE
210 220 230 240 250
YVVTRWYRAP ELLLNCSEYT AAIDIWSVGC ILGEIMTGQP LFPGKDYVHQ
260 270 280 290 300
LRLITELVGS PDNSSLGFLR SDNARRYVRQ LPRYPKQQFA ARFPKMPTTA
310 320 330 340 350
IDLLERMLVF DPNRRISVDE ALGHAYLSPH HDVAKEPVCS TPFSFDFEHP
360 370
SCTEEHIKEL IYKESVKFNP DH
Length:372
Mass (Da):42,475
Last modified:March 1, 2003 - v1
Checksum:i1A8FF099D1DC80B9
GO

Sequence cautioni

The sequence AAC62906 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005397 Genomic DNA Translation: AAC62906.1 Different initiation.
CP002685 Genomic DNA Translation: AEC10639.1
AK117449 mRNA Translation: BAC42114.1
BT024898 mRNA Translation: ABD85169.1
PIRiD84898
RefSeqiNP_001325006.1, NM_001337177.1
NP_182131.2, NM_130170.4 [Q8GYQ5-1]
UniGeneiAt.36555

Genome annotation databases

EnsemblPlantsiAT2G46070.1; AT2G46070.1; AT2G46070 [Q8GYQ5-1]
AT2G46070.3; AT2G46070.3; AT2G46070
GeneIDi819215
GrameneiAT2G46070.1; AT2G46070.1; AT2G46070 [Q8GYQ5-1]
AT2G46070.3; AT2G46070.3; AT2G46070
KEGGiath:AT2G46070

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMPK12_ARATH
AccessioniPrimary (citable) accession number: Q8GYQ5
Secondary accession number(s): O82361
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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