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Q8GYQ5

- MPK12_ARATH

UniProt

Q8GYQ5 - MPK12_ARATH

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Protein

Mitogen-activated protein kinase 12

Gene

MPK12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Negative regulator of the auxin transduction signaling pathway.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701ATPPROSITE-ProRule annotation
Active sitei167 – 1671Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 559ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: TAIR
  3. MAP kinase activity Source: TAIR

GO - Biological processi

  1. auxin-activated signaling pathway Source: UniProtKB-KW
  2. intracellular signal transduction Source: TAIR
  3. MAPK cascade Source: GOC
  4. response to auxin Source: TAIR
  5. response to indolebutyric acid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Auxin signaling pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G46070-MONOMER.
ARA:GQT-1132-MONOMER.
ReactomeiREACT_202229. ERK1 activation.
REACT_206508. ERK/MAPK targets.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_220111. Regulation of HSF1-mediated heat shock response.
REACT_245123. Signal transduction by L1.
REACT_247278. ISG15 antiviral mechanism.
REACT_251578. Signaling by FGFR.
REACT_268338. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 12 (EC:2.7.11.24)
Short name:
AtMPK12
Short name:
MAP kinase 12
Gene namesi
Name:MPK12
Ordered Locus Names:At2g46070
ORF Names:T3F17.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G46070.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Mitogen-activated protein kinase 12PRO_0000245812Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991PhosphothreonineBy similarity
Modified residuei201 – 2011PhosphotyrosineBy similarity
Modified residuei204 – 2041PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme (By similarity). Activated by auxin. Dephosphorylated and inactivated by IBR5.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8GYQ5.
PRIDEiQ8GYQ5.

Expressioni

Tissue specificityi

Expressed in seedlings, roots, stems, leaves, flowers and siliques, mostly around stomata.1 Publication

Gene expression databases

ExpressionAtlasiQ8GYQ5. baseline and differential.
GenevestigatoriQ8GYQ5.

Interactioni

Subunit structurei

Interacts with IBR5/SKIP33.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
IBR5Q84JU48EBI-2128461,EBI-604555
MKK1Q94A062EBI-2128461,EBI-994464
MKK6Q9FJV02EBI-2128461,EBI-1238868
MKK9Q9FX432EBI-2128461,EBI-2128545

Protein-protein interaction databases

BioGridi4550. 2 interactions.
IntActiQ8GYQ5. 6 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8GYQ5.
SMRiQ8GYQ5. Positions 6-370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 327287Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni210 – 372163IBR5 bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi199 – 2013TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ8GYQ5.
KOiK04371.
OMAiHAYLSPH.
PhylomeDBiQ8GYQ5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8GYQ5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGESSSGST EHCIKVVPTH GGRYVQYNVY GQLFEVSRKY VPPIRPIGRG
60 70 80 90 100
ACGIVCAAVN SVTGEKVAIK KIGNAFDNII DAKRTLREIK LLRHMDHENV
110 120 130 140 150
ITIKDIVRPP QRDIFNDVYI VYELMDTDLQ RILRSNQTLT SDQCRFLVYQ
160 170 180 190 200
LLRGLKYVHS ANILHRDLRP SNVLLNSKNE LKIGDFGLAR TTSDTDFMTE
210 220 230 240 250
YVVTRWYRAP ELLLNCSEYT AAIDIWSVGC ILGEIMTGQP LFPGKDYVHQ
260 270 280 290 300
LRLITELVGS PDNSSLGFLR SDNARRYVRQ LPRYPKQQFA ARFPKMPTTA
310 320 330 340 350
IDLLERMLVF DPNRRISVDE ALGHAYLSPH HDVAKEPVCS TPFSFDFEHP
360 370
SCTEEHIKEL IYKESVKFNP DH
Length:372
Mass (Da):42,475
Last modified:March 1, 2003 - v1
Checksum:i1A8FF099D1DC80B9
GO

Sequence cautioni

The sequence AAC62906.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005397 Genomic DNA. Translation: AAC62906.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC10639.1.
AK117449 mRNA. Translation: BAC42114.1.
BT024898 mRNA. Translation: ABD85169.1.
PIRiD84898.
RefSeqiNP_182131.2. NM_130170.3. [Q8GYQ5-1]
UniGeneiAt.36555.

Genome annotation databases

EnsemblPlantsiAT2G46070.1; AT2G46070.1; AT2G46070. [Q8GYQ5-1]
GeneIDi819215.
KEGGiath:AT2G46070.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005397 Genomic DNA. Translation: AAC62906.1 . Different initiation.
CP002685 Genomic DNA. Translation: AEC10639.1 .
AK117449 mRNA. Translation: BAC42114.1 .
BT024898 mRNA. Translation: ABD85169.1 .
PIRi D84898.
RefSeqi NP_182131.2. NM_130170.3. [Q8GYQ5-1 ]
UniGenei At.36555.

3D structure databases

ProteinModelPortali Q8GYQ5.
SMRi Q8GYQ5. Positions 6-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4550. 2 interactions.
IntActi Q8GYQ5. 6 interactions.

Proteomic databases

PaxDbi Q8GYQ5.
PRIDEi Q8GYQ5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G46070.1 ; AT2G46070.1 ; AT2G46070 . [Q8GYQ5-1 ]
GeneIDi 819215.
KEGGi ath:AT2G46070.

Organism-specific databases

GeneFarmi 856. 89.
TAIRi AT2G46070.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q8GYQ5.
KOi K04371.
OMAi HAYLSPH.
PhylomeDBi Q8GYQ5.

Enzyme and pathway databases

BioCyci ARA:AT2G46070-MONOMER.
ARA:GQT-1132-MONOMER.
Reactomei REACT_202229. ERK1 activation.
REACT_206508. ERK/MAPK targets.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_220111. Regulation of HSF1-mediated heat shock response.
REACT_245123. Signal transduction by L1.
REACT_247278. ISG15 antiviral mechanism.
REACT_251578. Signaling by FGFR.
REACT_268338. Signalling to ERK5.

Gene expression databases

ExpressionAtlasi Q8GYQ5. baseline and differential.
Genevestigatori Q8GYQ5.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: GENE FAMILY.
  7. "Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling."
    Lee J.S., Wang S., Sritubtim S., Chen J.-G., Ellis B.E.
    Plant J. 57:975-985(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ACTIVATION, INTERACTION WITH IBR5/SKIP33.

Entry informationi

Entry nameiMPK12_ARATH
AccessioniPrimary (citable) accession number: Q8GYQ5
Secondary accession number(s): O82361
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3