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Q8GYQ5 (MPK12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 12

Short name=AtMPK12
Short name=MAP kinase 12
EC=2.7.11.24
Gene names
Name:MPK12
Ordered Locus Names:At2g46070
ORF Names:T3F17.28
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of the auxin transduction signaling pathway. Ref.7

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity.

Subunit structure

Interacts with IBR5/SKIP33. Ref.7

Subcellular location

Nucleus Ref.7.

Tissue specificity

Expressed in seedlings, roots, stems, leaves, flowers and siliques, mostly around stomata. Ref.7

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme By similarity. Activated by auxin. Dephosphorylated and inactivated by IBR5.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAC62906.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8GYQ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Mitogen-activated protein kinase 12
PRO_0000245812

Regions

Domain41 – 327287Protein kinase
Nucleotide binding47 – 559ATP By similarity
Region210 – 372163IBR5 binding
Motif199 – 2013TXY

Sites

Active site1671Proton acceptor By similarity
Binding site701ATP By similarity

Amino acid modifications

Modified residue1991Phosphothreonine By similarity
Modified residue2011Phosphotyrosine By similarity
Modified residue2041Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 1A8FF099D1DC80B9

FASTA37242,475
        10         20         30         40         50         60 
MSGESSSGST EHCIKVVPTH GGRYVQYNVY GQLFEVSRKY VPPIRPIGRG ACGIVCAAVN 

        70         80         90        100        110        120 
SVTGEKVAIK KIGNAFDNII DAKRTLREIK LLRHMDHENV ITIKDIVRPP QRDIFNDVYI 

       130        140        150        160        170        180 
VYELMDTDLQ RILRSNQTLT SDQCRFLVYQ LLRGLKYVHS ANILHRDLRP SNVLLNSKNE 

       190        200        210        220        230        240 
LKIGDFGLAR TTSDTDFMTE YVVTRWYRAP ELLLNCSEYT AAIDIWSVGC ILGEIMTGQP 

       250        260        270        280        290        300 
LFPGKDYVHQ LRLITELVGS PDNSSLGFLR SDNARRYVRQ LPRYPKQQFA ARFPKMPTTA 

       310        320        330        340        350        360 
IDLLERMLVF DPNRRISVDE ALGHAYLSPH HDVAKEPVCS TPFSFDFEHP SCTEEHIKEL 

       370 
IYKESVKFNP DH 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Shinn P., Chen H., Kim C.J., Ecker J.R.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[7]"Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling."
Lee J.S., Wang S., Sritubtim S., Chen J.-G., Ellis B.E.
Plant J. 57:975-985(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ACTIVATION, INTERACTION WITH IBR5/SKIP33.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC005397 Genomic DNA. Translation: AAC62906.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC10639.1.
AK117449 mRNA. Translation: BAC42114.1.
BT024898 mRNA. Translation: ABD85169.1.
PIRD84898.
RefSeqNP_182131.2. NM_130170.3. [Q8GYQ5-1]
UniGeneAt.36555.

3D structure databases

ProteinModelPortalQ8GYQ5.
SMRQ8GYQ5. Positions 6-370.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4550. 2 interactions.
IntActQ8GYQ5. 6 interactions.

Proteomic databases

PaxDbQ8GYQ5.
PRIDEQ8GYQ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G46070.1; AT2G46070.1; AT2G46070. [Q8GYQ5-1]
GeneID819215.
KEGGath:AT2G46070.

Organism-specific databases

GeneFarm856. 89.
TAIRAT2G46070.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ8GYQ5.
KOK04371.
OMAHAYLSPH.
PhylomeDBQ8GYQ5.

Enzyme and pathway databases

BioCycARA:AT2G46070-MONOMER.
ARA:GQT-1132-MONOMER.

Gene expression databases

GenevestigatorQ8GYQ5.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK12_ARATH
AccessionPrimary (citable) accession number: Q8GYQ5
Secondary accession number(s): O82361
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names