ID   OPR2_ARATH              Reviewed;         374 AA.
AC   Q8GYB8; Q9ZR80;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 2.
DT   16-JUN-2009, entry version 51.
DE   RecName: Full=12-oxophytodienoate reductase 2;
DE            EC=1.3.1.42;
DE   AltName: Full=12-oxophytodienoate-10,11-reductase 2;
DE            Short=OPDA-reductase 2;
DE            Short=AtOPR2;
GN   Name=OPR2; OrderedLocusNames=At1g76690; ORFNames=F28O16.6;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, AND INDUCTION.
RC   STRAIN=cv. Columbia;
RX   MEDLINE=99266124; PubMed=10333582; DOI=10.1007/s004250050545;
RA   Biesgen C., Weiler E.W.;
RT   "Structure and regulation of OPR1 and OPR2, two closely related genes
RT   encoding 12-oxophytodienoic acid-10,11-reductases from Arabidopsis
RT   thaliana.";
RL   Planta 208:155-165(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21932900; PubMed=11910074; DOI=10.1126/science.1071006;
RA   Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA   Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M.,
RA   Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T.,
RA   Shibata K., Shinagawa A., Shinozaki K.;
RT   "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL   Science 296:141-145(2002).
RN   [4]
RP   SUBSTRATE SPECIFICITY.
RX   MEDLINE=20330991; PubMed=10872231; DOI=10.1007/s004250050706;
RA   Schaller F., Biesgen C., Muessig C., Altmann T., Weiler E.W.;
RT   "12-Oxophytodienoate reductase 3 (OPR3) is the isoenzyme involved in
RT   jasmonate biosynthesis.";
RL   Planta 210:979-984(2000).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   MEDLINE=22337585; PubMed=12445129;
RX   DOI=10.1046/j.1365-313X.2002.01449.x;
RA   Strassner J., Schaller F., Frick U.B., Howe G.A., Weiler E.W.,
RA   Amrhein N., Macheroux P., Schaller A.;
RT   "Characterization and cDNA-microarray expression analysis of 12-
RT   oxophytodienoate reductases reveals differential roles for
RT   octadecanoid biosynthesis in the local versus the systemic wound
RT   response.";
RL   Plant J. 32:585-601(2002).
CC   -!- FUNCTION: Specifically cleaves olefinic bonds in alpha,beta-
CC       unsaturated carbonyls and may be involved in detoxification or
CC       modification of these reactive compounds. May be involved in the
CC       biosynthesis or metabolism of oxylipin signaling molecules. In
CC       vitro, reduces 9R,13R-12-oxophyodienoic acid (9R,13R-OPDA) to
CC       9R,13R-OPC-8:0, but only poorly 9S,13S-OPDA, the natural precursor
CC       of jasmonic acid.
CC   -!- CATALYTIC ACTIVITY: 8-((1R,2R)-3-oxo-2-((Z)-pent-2-
CC       enyl)cyclopentyl)octanoate + NADP(+) = (15Z)-12-oxophyto-10,15-
CC       dienoate + NADPH.
CC   -!- COFACTOR: FMN (By similarity).
CC   -!- PATHWAY: Lipid metabolism; oxylipin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- TISSUE SPECIFICITY: Expressed at highest levels in roots and
CC       cotyledons, and at lower levels in leaves, shoots and flowers
CC       (sepals, petals, maturing siliques and developing pollen).
CC   -!- DEVELOPMENTAL STAGE: Expressed during late steps of pollen
CC       development.
CC   -!- INDUCTION: By wounding, locally and systemically, by cold and heat
CC       stresses, and by UV-C. Seems to not be influenced by UV-A and UV-
CC       B.
CC   -!- SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase
CC       family.
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DR   EMBL; U92460; AAC78441.1; -; Genomic_DNA.
DR   EMBL; AC010718; AAF04449.1; -; Genomic_DNA.
DR   EMBL; AK117738; BAC42387.1; -; mRNA.
DR   IPI; IPI00542803; -.
DR   PIR; C96795; C96795.
DR   RefSeq; NP_177795.1; -.
DR   UniGene; At.48382; -.
DR   HSSP; Q9XG54; 1ICP.
DR   SMR; Q8GYB8; 11-371.
DR   PRIDE; Q8GYB8; -.
DR   GeneID; 844002; -.
DR   GenomeReviews; CT485782_GR; AT1G76690.
DR   KEGG; ath:AT1G76690; -.
DR   NMPDR; fig|3702.1.peg.7196; -.
DR   GeneFarm; 4902; 471.
DR   TAIR; At1g76690; -.
DR   OMA; Q8GYB8; RISPSGE.
DR   BRENDA; 1.3.1.42; 302.
DR   GermOnline; AT1G76690; Arabidopsis thaliana.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016629; F:12-oxophytodienoate reductase activity; IEA:EC.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0031408; P:oxylipin biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009611; P:response to wounding; IEP:TAIR.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR001155; OxRdtase_FMN_N.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF00724; Oxidored_FMN; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Cytoplasm; Fatty acid biosynthesis; Flavoprotein;
KW   FMN; Lipid synthesis; NADP; Oxidoreductase; Oxylipin biosynthesis.
FT   CHAIN         1    374       12-oxophytodienoate reductase 2.
FT                                /FTId=PRO_0000194484.
FT   REGION      135    149       Substrate-binding loop (By similarity).
FT   REGION      140    141       Substrate-binding (By similarity).
FT   REGION      185    188       Substrate-binding (By similarity).
FT   ACT_SITE    190    190       Proton donor (By similarity).
FT   BINDING      35     35       FMN (By similarity).
FT   BINDING     108    108       FMN (By similarity).
FT   BINDING     237    237       FMN (By similarity).
FT   BINDING     329    329       FMN (By similarity).
FT   CONFLICT    174    174       A -> T (in Ref. 3; BAC42387).
SQ   SEQUENCE   374 AA;  41567 MW;  3168EDF4E857A883 CRC64;
     MEMVNAEAKQ SVPLLTPYKM GRFNLSHRVV LAPLTRQKSY GSVPQPHAIL YYSQRTSPGG
     FLIAEATGVS DTAQGYPDTP GIWTKEHVEA WKPIVDAVHA KGGIFFCQIW HVGRVSNRGF
     QPRRQAPISC TGKPIMPQMR ANGIDEARFT PPRRLSIEEI PGIVNDFRLA ARNAMEAGFD
     GVEIHGAHGY LIDQFMKDKV NDRTDEYGGS LQNRCKFALE VVDAVAKEIG PDRVGIRLSP
     FADYMESGDT NPEALGLYMV ESLNKYGILY CHMIEPRMKT VGEIAACSHT LMPMREAFKG
     TFISAGGFTR EDGNEAVAKG RTDLVAYGRW FLANPDLPKR FQLDAPLNKY NRSTFYTSDP
     VVGYTDYPSL ESTA
//