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Protein

Cysteine-rich receptor-like protein kinase 10

Gene

CRK10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei376ATPPROSITE-ProRule annotation1
Active sitei473Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi354 – 362ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine-rich receptor-like protein kinase 10 (EC:2.7.11.-)
Short name:
Cysteine-rich RLK10
Alternative name(s):
Receptor-like protein kinase 4
Gene namesi
Name:CRK10
Synonyms:RLK4
Ordered Locus Names:At4g23180
ORF Names:F21P8.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G23180.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini35 – 285ExtracellularSequence analysisAdd BLAST251
Transmembranei286 – 306HelicalSequence analysisAdd BLAST21
Topological domaini307 – 669CytoplasmicSequence analysisAdd BLAST363

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • plasmodesma Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Sequence analysisAdd BLAST34
ChainiPRO_000029505735 – 669Cysteine-rich receptor-like protein kinase 10Add BLAST635

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi49N-linked (GlcNAc...)Sequence analysis1
Glycosylationi53N-linked (GlcNAc...)Sequence analysis1
Glycosylationi71N-linked (GlcNAc...)Sequence analysis1
Glycosylationi80N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi96 ↔ 105PROSITE-ProRule annotation
Disulfide bondi108 ↔ 133PROSITE-ProRule annotation
Glycosylationi114N-linked (GlcNAc...)Sequence analysis1
Glycosylationi159N-linked (GlcNAc...)Sequence analysis1
Glycosylationi185N-linked (GlcNAc...)Sequence analysis1
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi209 ↔ 218PROSITE-ProRule annotation
Disulfide bondi221 ↔ 243PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8GYA4.
PRIDEiQ8GYA4.

PTM databases

iPTMnetiQ8GYA4.

Expressioni

Inductioni

By salicylic acid (SA) or by a bacterial pathogen infection. May be regulated by WRKY DNA-binding proteins at the transcriptional level.2 Publications

Gene expression databases

GenevisibleiQ8GYA4. AT.

Interactioni

Subunit structurei

Interacts with CRKIP1 (KAPP), CRKIP2 and CRKIP3, three kinase-associated type 2C proteins.1 Publication

Protein-protein interaction databases

BioGridi13706. 14 interactors.
IntActiQ8GYA4. 2 interactors.
STRINGi3702.AT4G23180.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GYA4.
SMRiQ8GYA4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 142Gnk2-homologous 1PROSITE-ProRule annotationAdd BLAST105
Domaini148 – 252Gnk2-homologous 2PROSITE-ProRule annotationAdd BLAST105
Domaini348 – 634Protein kinasePROSITE-ProRule annotationAdd BLAST287

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi259 – 276Pro-richPROSITE-ProRule annotationAdd BLAST18

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CRK subfamily.PROSITE-ProRule annotation
Contains 2 Gnk2-homologous domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJDF. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ8GYA4.
OMAiPIMALES.
OrthoDBiEOG09360DJ0.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR002902. GNK2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF01657. Stress-antifung. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51473. GNK2. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8GYA4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRNTDQESP IMSYYSSFFF LFLFSFLTSF RVSAQDPTYV YHTCQNTANY
60 70 80 90 100
TSNSTYNNNL KTLLASLSSR NASYSTGFQN ATVGQAPDRV TGLFNCRGDV
110 120 130 140 150
STEVCRRCVS FAVNDTLTRC PNQKEATLYY DECVLRYSNQ NILSTLITTG
160 170 180 190 200
GVILVNTRNV TSNQLDLLSD LVLPTLNQAA TVALNSSKKF GTRKNNFTAL
210 220 230 240 250
QSFYGLVQCT PDLTRQDCSR CLQLVINQIP TDRIGARIIN PSCTSRYEIY
260 270 280 290 300
AFYTESAVPP PPPPPSISTP PVSAPPRSGK DGNSKVLVIA IVVPIIVAVL
310 320 330 340 350
LFIAGYCFLT RRARKSYYTP SAFAGDDITT ADSLQLDYRT IQTATDDFVE
360 370 380 390 400
SNKIGQGGFG EVYKGTLSDG TEVAVKRLSK SSGQGEVEFK NEVVLVAKLQ
410 420 430 440 450
HRNLVRLLGF CLDGEERVLV YEYVPNKSLD YFLFDPAKKG QLDWTRRYKI
460 470 480 490 500
IGGVARGILY LHQDSRLTII HRDLKASNIL LDADMNPKIA DFGMARIFGL
510 520 530 540 550
DQTEENTSRI VGTYGYMSPE YAMHGQYSMK SDVYSFGVLV LEIISGKKNS
560 570 580 590 600
SFYQTDGAHD LVSYAWGLWS NGRPLELVDP AIVENCQRNE VVRCVHIGLL
610 620 630 640 650
CVQEDPAERP TLSTIVLMLT SNTVTLPVPR QPGLFFQSRI GKDPLDTDTT
660
SKSLLGSVDD ASITDIHPR
Length:669
Mass (Da):74,283
Last modified:May 31, 2011 - v3
Checksum:iE2CCD6AAC5F87BF7
GO
Isoform 2 (identifier: Q8GYA4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Note: No experimental confirmation available.
Show »
Length:658
Mass (Da):72,955
Checksum:iEFAC46D252902446
GO

Sequence cautioni

The sequence AAK28315 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA18465 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79273 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti268S → C in AAN60348 (Ref. 1) Curated1
Sequence conflicti279 – 281GKD → EKE in AAK28315 (PubMed:11135117).Curated3
Sequence conflicti300L → R in AAK28315 (PubMed:11135117).Curated1
Sequence conflicti318Y → S in AAK28315 (PubMed:11135117).Curated1
Sequence conflicti478N → T in AAK28315 (PubMed:11135117).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0413021 – 11Missing in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083790 mRNA. Translation: AAN60348.1.
AL022347 Genomic DNA. Translation: CAA18465.1. Sequence problems.
AL161558 Genomic DNA. Translation: CAB79273.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84719.1.
AK117765 mRNA. Translation: BAC42412.1.
BT005954 mRNA. Translation: AAO64889.1.
AF224705 mRNA. Translation: AAK28315.1. Different initiation.
PIRiT04835.
RefSeqiNP_567679.2. NM_118447.3. [Q8GYA4-1]
UniGeneiAt.19078.

Genome annotation databases

EnsemblPlantsiAT4G23180.1; AT4G23180.1; AT4G23180. [Q8GYA4-1]
GeneIDi828417.
GrameneiAT4G23180.1; AT4G23180.1; AT4G23180.
KEGGiath:AT4G23180.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083790 mRNA. Translation: AAN60348.1.
AL022347 Genomic DNA. Translation: CAA18465.1. Sequence problems.
AL161558 Genomic DNA. Translation: CAB79273.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84719.1.
AK117765 mRNA. Translation: BAC42412.1.
BT005954 mRNA. Translation: AAO64889.1.
AF224705 mRNA. Translation: AAK28315.1. Different initiation.
PIRiT04835.
RefSeqiNP_567679.2. NM_118447.3. [Q8GYA4-1]
UniGeneiAt.19078.

3D structure databases

ProteinModelPortaliQ8GYA4.
SMRiQ8GYA4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13706. 14 interactors.
IntActiQ8GYA4. 2 interactors.
STRINGi3702.AT4G23180.1.

PTM databases

iPTMnetiQ8GYA4.

Proteomic databases

PaxDbiQ8GYA4.
PRIDEiQ8GYA4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G23180.1; AT4G23180.1; AT4G23180. [Q8GYA4-1]
GeneIDi828417.
GrameneiAT4G23180.1; AT4G23180.1; AT4G23180.
KEGGiath:AT4G23180.

Organism-specific databases

TAIRiAT4G23180.

Phylogenomic databases

eggNOGiENOG410IJDF. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ8GYA4.
OMAiPIMALES.
OrthoDBiEOG09360DJ0.

Miscellaneous databases

PROiQ8GYA4.

Gene expression databases

GenevisibleiQ8GYA4. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR002902. GNK2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF01657. Stress-antifung. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51473. GNK2. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRK10_ARATH
AccessioniPrimary (citable) accession number: Q8GYA4
Secondary accession number(s): O65470, Q8H785, Q9C5T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: May 31, 2011
Last modified: November 30, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.