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Protein

Putative L-ascorbate peroxidase 6

Gene

APX6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a key role in hydrogen peroxide removal.By similarity

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei119 – 1191Transition state stabilizerPROSITE-ProRule annotation
Active sitei123 – 1231Proton acceptorPROSITE-ProRule annotation
Metal bindingi244 – 2441Iron (heme axial ligand)PROSITE-ProRule annotation

GO - Molecular functioni

  • heme binding Source: InterPro
  • L-ascorbate peroxidase activity Source: UniProtKB-EC
  • metal ion binding Source: UniProtKB-KW
  • peroxidase activity Source: TAIR

GO - Biological processi

  • hydrogen peroxide catabolic process Source: UniProtKB-KW
  • response to oxidative stress Source: InterPro
  • seed germination Source: TAIR
  • seed maturation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G32320-MONOMER.

Protein family/group databases

PeroxiBasei3952. AtAPx-R.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative L-ascorbate peroxidase 6 (EC:1.11.1.11)
Short name:
AtAPx08
Gene namesi
Name:APX6
Ordered Locus Names:At4g32320
ORF Names:F10M6.50, F8B4.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G32320.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Putative L-ascorbate peroxidase 6PRO_0000261325Add
BLAST

Proteomic databases

PaxDbiQ8GY91.
PRIDEiQ8GY91.

Expressioni

Gene expression databases

GenevisibleiQ8GY91. AT.

Interactioni

Protein-protein interaction databases

BioGridi14652. 1 interaction.
STRINGi3702.AT4G32320.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GY91.
SMRiQ8GY91. Positions 98-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IQ3Y. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ8GY91.
KOiK00434.
OMAiVDRPENT.
PhylomeDBiQ8GY91.

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GY91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTTASLVK TFLFRCDSFS SFKFKCKFES PAKTRLLSPA TEKHVVRSSR
60 70 80 90 100
AWRIRCLSDD PGSSHVFVAS RRKMVVLLST VQLLSHMLPQ NGNAAEIYPV
110 120 130 140 150
MQNEIRKVVT KGKAAGVLRL VFHDAGTFEL DDHSGGINGS IAYELERPEN
160 170 180 190 200
IGLKKSLKVL AKAKVKVDEI QPVSWADMIS VAGSEAVSIC GGPTIPVVLG
210 220 230 240 250
RLDSAQPDPE GKLPPETLSA SGLKECFKRK GFSTQELVAL SGAHTIGSKG
260 270 280 290 300
FGDPTVFDNA YYKILLEKPW TSTSKMTSMV GLPSDHALVQ DDECLRWVKR
310 320
YAEDQDKFFE DFTNAYIKLV NSGAKWNML
Length:329
Mass (Da):36,240
Last modified:March 1, 2003 - v1
Checksum:iBC50726AB7B689DD
GO

Sequence cautioni

The sequence CAA16959.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA22559.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79949.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021811 Genomic DNA. Translation: CAA16959.1. Sequence problems.
AL034567 Genomic DNA. Translation: CAA22559.1. Sequence problems.
AL161581 Genomic DNA. Translation: CAB79949.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86039.1.
AK117784 mRNA. Translation: BAC42431.1.
BT008349 mRNA. Translation: AAP37708.1.
PIRiT05342.
RefSeqiNP_194958.2. NM_119384.4.
UniGeneiAt.31673.

Genome annotation databases

EnsemblPlantsiAT4G32320.1; AT4G32320.1; AT4G32320.
GeneIDi829366.
GrameneiAT4G32320.1; AT4G32320.1; AT4G32320.
KEGGiath:AT4G32320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021811 Genomic DNA. Translation: CAA16959.1. Sequence problems.
AL034567 Genomic DNA. Translation: CAA22559.1. Sequence problems.
AL161581 Genomic DNA. Translation: CAB79949.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86039.1.
AK117784 mRNA. Translation: BAC42431.1.
BT008349 mRNA. Translation: AAP37708.1.
PIRiT05342.
RefSeqiNP_194958.2. NM_119384.4.
UniGeneiAt.31673.

3D structure databases

ProteinModelPortaliQ8GY91.
SMRiQ8GY91. Positions 98-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14652. 1 interaction.
STRINGi3702.AT4G32320.1.

Protein family/group databases

PeroxiBasei3952. AtAPx-R.

Proteomic databases

PaxDbiQ8GY91.
PRIDEiQ8GY91.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G32320.1; AT4G32320.1; AT4G32320.
GeneIDi829366.
GrameneiAT4G32320.1; AT4G32320.1; AT4G32320.
KEGGiath:AT4G32320.

Organism-specific databases

TAIRiAT4G32320.

Phylogenomic databases

eggNOGiENOG410IQ3Y. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ8GY91.
KOiK00434.
OMAiVDRPENT.
PhylomeDBiQ8GY91.

Enzyme and pathway databases

BioCyciARA:AT4G32320-MONOMER.

Miscellaneous databases

PROiQ8GY91.

Gene expression databases

GenevisibleiQ8GY91. AT.

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiAPX6_ARATH
AccessioniPrimary (citable) accession number: Q8GY91
Secondary accession number(s): O49360
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.