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Protein

Probable ubiquitin-conjugating enzyme E2 26

Gene

UBC26

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei357 – 3571Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G53025-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin-conjugating enzyme E2 26 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 26
Gene namesi
Name:UBC26
Ordered Locus Names:At1g53025
ORF Names:F8L10.11, F8L10.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G53025.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 543543Probable ubiquitin-conjugating enzyme E2 26PRO_0000345191Add
BLAST

Proteomic databases

PaxDbiQ8GY87.

Interactioni

Protein-protein interaction databases

IntActiQ8GY87. 3 interactions.
STRINGi3702.AT1G53025.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GY87.
SMRiQ8GY87. Positions 236-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 124Poly-Pro

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000237682.
InParanoidiQ8GY87.
KOiK10581.
OMAiWAGSARE.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GY87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPDVVEIPP PPLIASGSRT RKPRKAVPEV IDVESYEFRN VGVVKDNNVV
60 70 80 90 100
DKKNKGKAIQ VDSFSFNNVQ SHHHGSSLLN LETFQDYYGH KNIPFSEFAN
110 120 130 140 150
QPIDVDDYSM YQDVLDPKDV PAGAEVTVPW GLNSSSKGTA KSSISIMRSQ
160 170 180 190 200
SMKGYGTVSL ATTNVPQLWD YTLPQQNQAI YSSVSFSAVQ PQTPDVVMVT
210 220 230 240 250
NPTPNPFSYD ASASSSHPIA AEPISSVQDS SNARKLKEEF LRDFKRFDTV
260 270 280 290 300
EDFSDHHYAS KGKSSKQHSK NWVKKVQADW KILENDLPEA ISVRACESRM
310 320 330 340 350
DLLRAVIIGA EGTPYHDGLF FFDIQFPDTY PSVPPNVHYH SGGLRINPNL
360 370 380 390 400
YNCGKVCLSL LGTWAGSARE KWLPNESTML QLLVSIQALI LNEKPYFNEP
410 420 430 440 450
GYVQSAGTAS GESKSKVYSE NVFLLSLKTM VYSIRRPPQH FEEYVQNHYF
460 470 480 490 500
VRSHDIVKAC NAYKAGAPLG SMVKGGVQDL EEARQSGSKK FKTDVASFMQ
510 520 530 540
TVVDEFVKLG VKELAEKPEP PMSNANTENQ SKKKTRKRSR SSR
Length:543
Mass (Da):60,552
Last modified:July 22, 2008 - v2
Checksum:i91B327FE466403C6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181K → E in BAC42437 (PubMed:11910074).Curated
Sequence conflicti118 – 1181K → E in AAO64853 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027039 mRNA. Translation: AAY44865.1.
AC022520 Genomic DNA. Translation: AAF87864.1.
CP002684 Genomic DNA. Translation: AEE32878.1.
AK117792 mRNA. Translation: BAC42437.1.
BT005918 mRNA. Translation: AAO64853.1.
AK176042 mRNA. Translation: BAD43805.1.
PIRiG96570.
RefSeqiNP_001185207.1. NM_001198278.1.
UniGeneiAt.37482.

Genome annotation databases

EnsemblPlantsiAT1G53025.1; AT1G53025.1; AT1G53025.
GeneIDi10723113.
KEGGiath:AT1G53025.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027039 mRNA. Translation: AAY44865.1.
AC022520 Genomic DNA. Translation: AAF87864.1.
CP002684 Genomic DNA. Translation: AEE32878.1.
AK117792 mRNA. Translation: BAC42437.1.
BT005918 mRNA. Translation: AAO64853.1.
AK176042 mRNA. Translation: BAD43805.1.
PIRiG96570.
RefSeqiNP_001185207.1. NM_001198278.1.
UniGeneiAt.37482.

3D structure databases

ProteinModelPortaliQ8GY87.
SMRiQ8GY87. Positions 236-465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8GY87. 3 interactions.
STRINGi3702.AT1G53025.1.

Proteomic databases

PaxDbiQ8GY87.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G53025.1; AT1G53025.1; AT1G53025.
GeneIDi10723113.
KEGGiath:AT1G53025.

Organism-specific databases

TAIRiAT1G53025.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000237682.
InParanoidiQ8GY87.
KOiK10581.
OMAiWAGSARE.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:AT1G53025-MONOMER.

Miscellaneous databases

PROiQ8GY87.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBC26_ARATH
AccessioniPrimary (citable) accession number: Q8GY87
Secondary accession number(s): Q9LNM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 22, 2008
Last modified: June 24, 2015
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.