Q8GY87 (UBC26_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin-conjugating enzyme E2 26
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 26

Gene names

Name:	UBC26
Ordered Locus Names:	At1g53025
ORF Names:	F8L10.11, F8L10.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Probable ubiquitin-conjugating enzyme E2 26		PRO_0000345191
Regions
	Compositional bias	9 – 12	4	Poly-Pro
Sites
	Active site	357	1	Glycyl thioester intermediate By similarity
Experimental info
	Sequence conflict	118	1	K → E in BAC42437. Ref.4
	Sequence conflict	118	1	K → E in AAO64853. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q8GY87 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 91B327FE466403C6	FASTA	543	60,552
	10 20 30 40 50 60 MEPDVVEIPP PPLIASGSRT RKPRKAVPEV IDVESYEFRN VGVVKDNNVV DKKNKGKAIQ 70 80 90 100 110 120 VDSFSFNNVQ SHHHGSSLLN LETFQDYYGH KNIPFSEFAN QPIDVDDYSM YQDVLDPKDV 130 140 150 160 170 180 PAGAEVTVPW GLNSSSKGTA KSSISIMRSQ SMKGYGTVSL ATTNVPQLWD YTLPQQNQAI 190 200 210 220 230 240 YSSVSFSAVQ PQTPDVVMVT NPTPNPFSYD ASASSSHPIA AEPISSVQDS SNARKLKEEF 250 260 270 280 290 300 LRDFKRFDTV EDFSDHHYAS KGKSSKQHSK NWVKKVQADW KILENDLPEA ISVRACESRM 310 320 330 340 350 360 DLLRAVIIGA EGTPYHDGLF FFDIQFPDTY PSVPPNVHYH SGGLRINPNL YNCGKVCLSL 370 380 390 400 410 420 LGTWAGSARE KWLPNESTML QLLVSIQALI LNEKPYFNEP GYVQSAGTAS GESKSKVYSE 430 440 450 460 470 480 NVFLLSLKTM VYSIRRPPQH FEEYVQNHYF VRSHDIVKAC NAYKAGAPLG SMVKGGVQDL 490 500 510 520 530 540 EEARQSGSKK FKTDVASFMQ TVVDEFVKLG VKELAEKPEP PMSNANTENQ SKKKTRKRSR SSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ027039 mRNA. Translation: AAY44865.1. AC022520 Genomic DNA. Translation: AAF87864.1. CP002684 Genomic DNA. Translation: AEE32878.1. AK117792 mRNA. Translation: BAC42437.1. BT005918 mRNA. Translation: AAO64853.1. AK176042 mRNA. Translation: BAD43805.1.
IPI	IPI01020380.
PIR	G96570.
RefSeq	NP_001185207.1. NM_001198278.1.
UniGene	At.37482.
3D structure databases
HSSP	HSSP built from PDB template 1JAS based on UniProtKB P63146.
ProteinModelPortal	Q8GY87.
SMR	Q8GY87. Positions 236-465.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8GY87. 3 interactions.
STRING	3702.AT1G53020.1-P.
Proteomic databases
PaxDb	Q8GY87.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G53025.1; AT1G53025.1; AT1G53025.
GeneID	10723113.
KEGG	ath:AT1G53025.
Organism-specific databases
TAIR	At1g53025.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000237682.
KO	K10581.
OMA	WAGSARE.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q8GY87.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC26_ARATH

Accession

Primary (citable) accession number: Q8GY87
Secondary accession number(s): Q9LNM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program