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Protein

Transcription factor bHLH63

Gene

BHLH63

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds DNA to G box 5'-CACGTG-3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light.2 Publications

GO - Molecular functioni

  • DNA binding Source: TAIR
  • protein dimerization activity Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • flower development Source: UniProtKB-KW
  • positive regulation of flower development Source: TAIR
  • response to blue light Source: UniProtKB
  • transcription, DNA-templated Source: TAIR

Keywordsi

Molecular functionActivator, DNA-binding
Biological processFlowering, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor bHLH63
Alternative name(s):
Basic helix-loop-helix protein 63
Short name:
AtbHLH63
Short name:
bHLH 63
Protein CRYPTOCHROME INTERACTING BASIC-HELIX-LOOP-HELIX 1
Transcription factor EN 84
bHLH transcription factor bHLH063
Gene namesi
Name:BHLH63
Synonyms:CIB1, EN84
Ordered Locus Names:At4g34530
ORF Names:T4L20.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G34530.
TAIRilocus:2139484. AT4G34530.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Biotechnological usei

The blue light-mediated interaction between CRY2 and BHLH63/CIB1 is used to design an optogenetic control of target proteins or organelles.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003587581 – 335Transcription factor bHLH63Add BLAST335

Proteomic databases

PaxDbiQ8GY61.
PRIDEiQ8GY61.

Expressioni

Tissue specificityi

Expressed constitutively in roots, leaves, and stems.1 Publication

Inductioni

Accumulates strongly in response to blue light due to reduced preventing 26S proteasome-mediated degradation in an ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in the absence of blue light via 26S proteasome degradation (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer (Probable). Interacts with IBH1 (PubMed:23161888). Binds reversibly to CRY2 after blue light illumination (PubMed:18988809, PubMed:24780222, PubMed:24130508).Curated4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi14886. 6 interactors.
DIPiDIP-59356N.
IntActiQ8GY61. 36 interactors.
STRINGi3702.AT4G34530.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GY61.
SMRiQ8GY61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 228bHLHPROSITE-ProRule annotationAdd BLAST51

Phylogenomic databases

eggNOGiENOG410IGTK. Eukaryota.
ENOG410YGAM. LUCA.
HOGENOMiHOG000238007.
InParanoidiQ8GY61.
OMAiGYSHEMV.
OrthoDBiEOG09360J45.
PhylomeDBiQ8GY61.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
PfamiView protein in Pfam
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GY61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGAIGGDLL LNFPDMSVLE RQRAHLKYLN PTFDSPLAGF FADSSMITGG
60 70 80 90 100
EMDSYLSTAG LNLPMMYGET TVEGDSRLSI SPETTLGTGN FKKRKFDTET
110 120 130 140 150
KDCNEKKKKM TMNRDDLVEE GEEEKSKITE QNNGSTKSIK KMKHKAKKEE
160 170 180 190 200
NNFSNDSSKV TKELEKTDYI HVRARRGQAT DSHSIAERVR REKISERMKF
210 220 230 240 250
LQDLVPGCDK ITGKAGMLDE IINYVQSLQR QIEFLSMKLA IVNPRPDFDM
260 270 280 290 300
DDIFAKEVAS TPMTVVPSPE MVLSGYSHEM VHSGYSSEMV NSGYLHVNPM
310 320 330
QQVNTSSDPL SCFNNGEAPS MWDSHVQNLY GNLGV
Length:335
Mass (Da):37,540
Last modified:March 1, 2003 - v1
Checksum:i05F0CA14FC2B3C7A
GO

Sequence cautioni

The sequence CAA18832 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80170 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86L → F in AAM53299 (PubMed:14593172).Curated1
Sequence conflicti110M → V in AAM10952 (PubMed:12679534).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF488596 mRNA. Translation: AAM10952.1.
AL023094 Genomic DNA. Translation: CAA18832.1. Sequence problems.
AL161585 Genomic DNA. Translation: CAB80170.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86389.1.
AK117846 mRNA. Translation: BAC42487.1.
AY120741 mRNA. Translation: AAM53299.1.
BT005313 mRNA. Translation: AAO63377.1.
PIRiT05273.
RefSeqiNP_195179.2. NM_119618.3.
UniGeneiAt.48937.

Genome annotation databases

EnsemblPlantsiAT4G34530.1; AT4G34530.1; AT4G34530.
GeneIDi829604.
GrameneiAT4G34530.1; AT4G34530.1; AT4G34530.
KEGGiath:AT4G34530.

Similar proteinsi

Entry informationi

Entry nameiBH063_ARATH
AccessioniPrimary (citable) accession number: Q8GY61
Secondary accession number(s): O65678, Q8L851, Q8S3D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: March 1, 2003
Last modified: September 27, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names